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Protein

Acetolactate synthase small subunit, mitochondrial

Gene

ILV6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates activity of the acetolactate synthase catalytic subunit ILV2 seven- to tenfold and confers sensitivity to inhibition by valine and activation by ATP.1 Publication

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit, mitochondrial (ILV6), Acetolactate synthase catalytic subunit, mitochondrial (ILV2)
  2. Ketol-acid reductoisomerase, mitochondrial (ILV5)
  3. Dihydroxy-acid dehydratase, mitochondrial (ILV3)
  4. Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Branched-chain-amino-acid aminotransferase, cytosolic (BAT2)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit, mitochondrial (ILV6), Acetolactate synthase catalytic subunit, mitochondrial (ILV2)
  2. Ketol-acid reductoisomerase, mitochondrial (ILV5)
  3. Dihydroxy-acid dehydratase, mitochondrial (ILV3)
  4. Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Branched-chain-amino-acid aminotransferase, cytosolic (BAT2)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • acetolactate synthase activity Source: SGD
  • amino acid binding Source: InterPro
  • enzyme regulator activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YCL009C-MONOMER.
UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase small subunit, mitochondrial
Alternative name(s):
Acetohydroxy-acid synthase small subunit
Short name:
AHAS
Short name:
ALS
Gene namesi
Name:ILV6
Ordered Locus Names:YCL009C
ORF Names:YCL9C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL009C.
SGDiS000000515. ILV6.

Subcellular locationi

GO - Cellular componenti

  • acetolactate synthase complex Source: SGD
  • mitochondrial nucleoid Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2424Mitochondrion1 PublicationAdd
BLAST
Chaini25 – 309285Acetolactate synthase small subunit, mitochondrialPRO_0000015637Add
BLAST

Proteomic databases

MaxQBiP25605.

Interactioni

Subunit structurei

The acetolactate synthase complex contains the catalytic regulatory subunit ILV2 and the regulatory small subunit ILV6.

Protein-protein interaction databases

BioGridi30972. 54 interactions.
DIPiDIP-671N.
IntActiP25605. 18 interactions.
MINTiMINT-522376.

Structurei

3D structure databases

ProteinModelPortaliP25605.
SMRiP25605. Positions 77-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini79 – 15981ACTPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi25 – 306Poly-Ser

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000046747.
InParanoidiP25605.
KOiK01653.
OMAiHKHEHLK.
OrthoDBiEOG092C3P3Q.

Family and domain databases

InterProiIPR004789. Acetalactate_synth_ssu.
IPR019455. Acetolactate_synth_ssu_C.
IPR002912. ACT_dom.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF10369. ALS_ss_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00119. acolac_sm. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRSLLQSGH RRVVASSCAT MVRCSSSSTS ALAYKQMHRH ATRPPLPTLD
60 70 80 90 100
TPSWNANSAV SSIIYETPAP SRQPRKQHVL NCLVQNEPGV LSRVSGTLAA
110 120 130 140 150
RGFNIDSLVV CNTEVKDLSR MTIVLQGQDG VVEQARRQIE DLVPVYAVLD
160 170 180 190 200
YTNSEIIKRE LVMARISLLG TEYFEDLLLH HHTSTNAGAA DSQELVAEIR
210 220 230 240 250
EKQFHPANLP ASEVLRLKHE HLNDITNLTN NFGGRVVDIS ETSCIVELSA
260 270 280 290 300
KPTRISAFLK LVEPFGVLEC ARSGMMALPR TPLKTSTEEA ADEDEKISEI

VDISQLPPG
Length:309
Mass (Da):33,987
Last modified:July 15, 1998 - v2
Checksum:iBF36073D89BC0065
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42350.1.
BK006937 Genomic DNA. Translation: DAA07472.1.
PIRiS19411.
RefSeqiNP_009918.1. NM_001178658.1.

Genome annotation databases

EnsemblFungiiCAA42350; CAA42350; CAA42350.
YCL009C; YCL009C; YCL009C.
GeneIDi850348.
KEGGisce:YCL009C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42350.1.
BK006937 Genomic DNA. Translation: DAA07472.1.
PIRiS19411.
RefSeqiNP_009918.1. NM_001178658.1.

3D structure databases

ProteinModelPortaliP25605.
SMRiP25605. Positions 77-280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30972. 54 interactions.
DIPiDIP-671N.
IntActiP25605. 18 interactions.
MINTiMINT-522376.

Proteomic databases

MaxQBiP25605.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42350; CAA42350; CAA42350.
YCL009C; YCL009C; YCL009C.
GeneIDi850348.
KEGGisce:YCL009C.

Organism-specific databases

EuPathDBiFungiDB:YCL009C.
SGDiS000000515. ILV6.

Phylogenomic databases

HOGENOMiHOG000046747.
InParanoidiP25605.
KOiK01653.
OMAiHKHEHLK.
OrthoDBiEOG092C3P3Q.

Enzyme and pathway databases

UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.
BioCyciYEAST:YCL009C-MONOMER.

Miscellaneous databases

PROiP25605.

Family and domain databases

InterProiIPR004789. Acetalactate_synth_ssu.
IPR019455. Acetolactate_synth_ssu_C.
IPR002912. ACT_dom.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF10369. ALS_ss_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00119. acolac_sm. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiILV6_YEAST
AccessioniPrimary (citable) accession number: P25605
Secondary accession number(s): D6VR03, P87009
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 15, 1998
Last modified: September 7, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.