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P25605 (ILV6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acetolactate synthase small subunit, mitochondrial
Alternative name(s):
Acetohydroxy-acid synthase small subunit
Short name=AHAS
Short name=ALS
Gene names
Name:ILV6
Ordered Locus Names:YCL009C
ORF Names:YCL9C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stimulates activity of the acetolactate synthase catalytic subunit ILV2 seven- to tenfold and confers sensitivity to inhibition by valine and activation by ATP. Ref.7

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.

Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.

Subunit structure

The acetolactate synthase complex contains the catalytic regulatory subunit ILV2 and the regulatory small subunit ILV6. Ref.4

Subcellular location

Mitochondrion.

Miscellaneous

Present with 15800 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the acetolactate synthase small subunit family.

Contains 1 ACT domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-9087,EBI-9087
SSA2P105921EBI-9087,EBI-8603

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2424Mitochondrion Ref.5
Chain25 – 309285Acetolactate synthase small subunit, mitochondrial
PRO_0000015637

Regions

Domain79 – 15981ACT
Compositional bias25 – 306Poly-Ser

Sequences

Sequence LengthMass (Da)Tools
P25605 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: BF36073D89BC0065

FASTA30933,987
        10         20         30         40         50         60 
MLRSLLQSGH RRVVASSCAT MVRCSSSSTS ALAYKQMHRH ATRPPLPTLD TPSWNANSAV 

        70         80         90        100        110        120 
SSIIYETPAP SRQPRKQHVL NCLVQNEPGV LSRVSGTLAA RGFNIDSLVV CNTEVKDLSR 

       130        140        150        160        170        180 
MTIVLQGQDG VVEQARRQIE DLVPVYAVLD YTNSEIIKRE LVMARISLLG TEYFEDLLLH 

       190        200        210        220        230        240 
HHTSTNAGAA DSQELVAEIR EKQFHPANLP ASEVLRLKHE HLNDITNLTN NFGGRVVDIS 

       250        260        270        280        290        300 
ETSCIVELSA KPTRISAFLK LVEPFGVLEC ARSGMMALPR TPLKTSTEEA ADEDEKISEI 


VDISQLPPG 

« Hide

References

« Hide 'large scale' references
[1]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Gromadka R.
Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 300.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Comprehensive sequence analysis of the 182 predicted open reading frames of yeast chromosome III."
Bork P., Ouzounis C., Sander C., Scharf M., Schneider R., Sonnhammer E.
Protein Sci. 1:1677-1690(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY TO ACETOLACTATE SYNTHASE SMALL SUBUNITS.
[5]"Two-dimensional protein map of Saccharomyces cerevisiae: construction of a gene-protein index."
Boucherie H., Dujardin G., Kermorgant M., Monribot C., Slonimski P.P., Perrot M.
Yeast 11:601-613(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-44.
Strain: ATCC 204508 / S288c.
[6]"Functional analysis of YCL09C: evidence for a role as the regulatory subunit of acetolactate synthase."
Cullin C., Baudin-Baillieu A., Guillemet E., Ozier-Kalogeropoulos O.
Yeast 12:1511-1518(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[7]"Expression, purification, characterization, and reconstitution of the large and small subunits of yeast acetohydroxyacid synthase."
Pang S.S., Duggleby R.G.
Biochemistry 38:5222-5231(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, RECONSTITUTION OF THE ACETOLACTATE SYNTHASE COMPLEX.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59720 Genomic DNA. Translation: CAA42350.1.
BK006937 Genomic DNA. Translation: DAA07472.1.
PIRS19411.
RefSeqNP_009918.1. NM_001178658.1.

3D structure databases

ProteinModelPortalP25605.
SMRP25605. Positions 77-280.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid30972. 52 interactions.
DIPDIP-671N.
IntActP25605. 17 interactions.
MINTMINT-522376.
STRING4932.YCL009C.

Proteomic databases

PaxDbP25605.
PeptideAtlasP25605.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCL009C; YCL009C; YCL009C.
GeneID850348.
KEGGsce:YCL009C.

Organism-specific databases

CYGDYCL009c.
SGDS000000515. ILV6.

Phylogenomic databases

eggNOGCOG0440.
HOGENOMHOG000046747.
KOK01653.
OMAGMMALPR.
OrthoDBEOG7CCC29.

Enzyme and pathway databases

BioCycYEAST:YCL009C-MONOMER.
UniPathwayUPA00047; UER00055.
UPA00049; UER00059.

Gene expression databases

GenevestigatorP25605.

Family and domain databases

InterProIPR004789. Acetalactate_synth_ssu.
IPR019455. Acetolactate_synth_ssu_C.
IPR002912. ACT_dom.
[Graphical view]
PfamPF01842. ACT. 1 hit.
PF10369. ALS_ss_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR00119. acolac_sm. 1 hit.
PROSITEPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio965806.

Entry information

Entry nameILV6_YEAST
AccessionPrimary (citable) accession number: P25605
Secondary accession number(s): D6VR03, P87009
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 15, 1998
Last modified: April 16, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways