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P25605

- ILV6_YEAST

UniProt

P25605 - ILV6_YEAST

Protein

Acetolactate synthase small subunit, mitochondrial

Gene

ILV6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Stimulates activity of the acetolactate synthase catalytic subunit ILV2 seven- to tenfold and confers sensitivity to inhibition by valine and activation by ATP.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. acetolactate synthase activity Source: SGD
    2. amino acid binding Source: InterPro
    3. enzyme regulator activity Source: SGD

    GO - Biological processi

    1. branched-chain amino acid biosynthetic process Source: SGD
    2. isoleucine biosynthetic process Source: UniProtKB-UniPathway
    3. regulation of catalytic activity Source: GOC
    4. valine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:YCL009C-MONOMER.
    UniPathwayiUPA00047; UER00055.
    UPA00049; UER00059.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetolactate synthase small subunit, mitochondrial
    Alternative name(s):
    Acetohydroxy-acid synthase small subunit
    Short name:
    AHAS
    Short name:
    ALS
    Gene namesi
    Name:ILV6
    Ordered Locus Names:YCL009C
    ORF Names:YCL9C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCL009c.
    SGDiS000000515. ILV6.

    Subcellular locationi

    GO - Cellular componenti

    1. acetolactate synthase complex Source: SGD
    2. mitochondrial nucleoid Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2424Mitochondrion1 PublicationAdd
    BLAST
    Chaini25 – 309285Acetolactate synthase small subunit, mitochondrialPRO_0000015637Add
    BLAST

    Proteomic databases

    MaxQBiP25605.
    PaxDbiP25605.
    PeptideAtlasiP25605.

    Expressioni

    Gene expression databases

    GenevestigatoriP25605.

    Interactioni

    Subunit structurei

    The acetolactate synthase complex contains the catalytic regulatory subunit ILV2 and the regulatory small subunit ILV6.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-9087,EBI-9087
    SSA2P105921EBI-9087,EBI-8603

    Protein-protein interaction databases

    BioGridi30972. 52 interactions.
    DIPiDIP-671N.
    IntActiP25605. 18 interactions.
    MINTiMINT-522376.
    STRINGi4932.YCL009C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25605.
    SMRiP25605. Positions 77-280.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini79 – 15981ACTPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi25 – 306Poly-Ser

    Sequence similaritiesi

    Contains 1 ACT domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0440.
    HOGENOMiHOG000046747.
    KOiK01653.
    OMAiGMMALPR.
    OrthoDBiEOG7CCC29.

    Family and domain databases

    InterProiIPR004789. Acetalactate_synth_ssu.
    IPR019455. Acetolactate_synth_ssu_C.
    IPR002912. ACT_dom.
    [Graphical view]
    PfamiPF01842. ACT. 1 hit.
    PF10369. ALS_ss_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00119. acolac_sm. 1 hit.
    PROSITEiPS51671. ACT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25605-1 [UniParc]FASTAAdd to Basket

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    MLRSLLQSGH RRVVASSCAT MVRCSSSSTS ALAYKQMHRH ATRPPLPTLD    50
    TPSWNANSAV SSIIYETPAP SRQPRKQHVL NCLVQNEPGV LSRVSGTLAA 100
    RGFNIDSLVV CNTEVKDLSR MTIVLQGQDG VVEQARRQIE DLVPVYAVLD 150
    YTNSEIIKRE LVMARISLLG TEYFEDLLLH HHTSTNAGAA DSQELVAEIR 200
    EKQFHPANLP ASEVLRLKHE HLNDITNLTN NFGGRVVDIS ETSCIVELSA 250
    KPTRISAFLK LVEPFGVLEC ARSGMMALPR TPLKTSTEEA ADEDEKISEI 300
    VDISQLPPG 309
    Length:309
    Mass (Da):33,987
    Last modified:July 15, 1998 - v2
    Checksum:iBF36073D89BC0065
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42350.1.
    BK006937 Genomic DNA. Translation: DAA07472.1.
    PIRiS19411.
    RefSeqiNP_009918.1. NM_001178658.1.

    Genome annotation databases

    EnsemblFungiiYCL009C; YCL009C; YCL009C.
    GeneIDi850348.
    KEGGisce:YCL009C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42350.1 .
    BK006937 Genomic DNA. Translation: DAA07472.1 .
    PIRi S19411.
    RefSeqi NP_009918.1. NM_001178658.1.

    3D structure databases

    ProteinModelPortali P25605.
    SMRi P25605. Positions 77-280.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30972. 52 interactions.
    DIPi DIP-671N.
    IntActi P25605. 18 interactions.
    MINTi MINT-522376.
    STRINGi 4932.YCL009C.

    Proteomic databases

    MaxQBi P25605.
    PaxDbi P25605.
    PeptideAtlasi P25605.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCL009C ; YCL009C ; YCL009C .
    GeneIDi 850348.
    KEGGi sce:YCL009C.

    Organism-specific databases

    CYGDi YCL009c.
    SGDi S000000515. ILV6.

    Phylogenomic databases

    eggNOGi COG0440.
    HOGENOMi HOG000046747.
    KOi K01653.
    OMAi GMMALPR.
    OrthoDBi EOG7CCC29.

    Enzyme and pathway databases

    UniPathwayi UPA00047 ; UER00055 .
    UPA00049 ; UER00059 .
    BioCyci YEAST:YCL009C-MONOMER.

    Miscellaneous databases

    NextBioi 965806.

    Gene expression databases

    Genevestigatori P25605.

    Family and domain databases

    InterProi IPR004789. Acetalactate_synth_ssu.
    IPR019455. Acetolactate_synth_ssu_C.
    IPR002912. ACT_dom.
    [Graphical view ]
    Pfami PF01842. ACT. 1 hit.
    PF10369. ALS_ss_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00119. acolac_sm. 1 hit.
    PROSITEi PS51671. ACT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Gromadka R.
      Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 300.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Comprehensive sequence analysis of the 182 predicted open reading frames of yeast chromosome III."
      Bork P., Ouzounis C., Sander C., Scharf M., Schneider R., Sonnhammer E.
      Protein Sci. 1:1677-1690(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: SIMILARITY TO ACETOLACTATE SYNTHASE SMALL SUBUNITS.
    5. "Two-dimensional protein map of Saccharomyces cerevisiae: construction of a gene-protein index."
      Boucherie H., Dujardin G., Kermorgant M., Monribot C., Slonimski P.P., Perrot M.
      Yeast 11:601-613(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-44.
      Strain: ATCC 204508 / S288c.
    6. "Functional analysis of YCL09C: evidence for a role as the regulatory subunit of acetolactate synthase."
      Cullin C., Baudin-Baillieu A., Guillemet E., Ozier-Kalogeropoulos O.
      Yeast 12:1511-1518(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. "Expression, purification, characterization, and reconstitution of the large and small subunits of yeast acetohydroxyacid synthase."
      Pang S.S., Duggleby R.G.
      Biochemistry 38:5222-5231(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, RECONSTITUTION OF THE ACETOLACTATE SYNTHASE COMPLEX.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiILV6_YEAST
    AccessioniPrimary (citable) accession number: P25605
    Secondary accession number(s): D6VR03, P87009
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 15800 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3