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P25605

- ILV6_YEAST

UniProt

P25605 - ILV6_YEAST

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Protein

Acetolactate synthase small subunit, mitochondrial

Gene

ILV6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Stimulates activity of the acetolactate synthase catalytic subunit ILV2 seven- to tenfold and confers sensitivity to inhibition by valine and activation by ATP.1 Publication

Pathwayi

GO - Molecular functioni

  1. acetolactate synthase activity Source: SGD
  2. amino acid binding Source: InterPro
  3. enzyme regulator activity Source: SGD

GO - Biological processi

  1. branched-chain amino acid biosynthetic process Source: SGD
  2. isoleucine biosynthetic process Source: UniProtKB-UniPathway
  3. regulation of catalytic activity Source: GOC
  4. valine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YCL009C-MONOMER.
UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase small subunit, mitochondrial
Alternative name(s):
Acetohydroxy-acid synthase small subunit
Short name:
AHAS
Short name:
ALS
Gene namesi
Name:ILV6
Ordered Locus Names:YCL009C
ORF Names:YCL9C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCL009c.
SGDiS000000515. ILV6.

Subcellular locationi

GO - Cellular componenti

  1. acetolactate synthase complex Source: SGD
  2. mitochondrial nucleoid Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2424Mitochondrion1 PublicationAdd
BLAST
Chaini25 – 309285Acetolactate synthase small subunit, mitochondrialPRO_0000015637Add
BLAST

Proteomic databases

MaxQBiP25605.
PaxDbiP25605.
PeptideAtlasiP25605.

Expressioni

Gene expression databases

GenevestigatoriP25605.

Interactioni

Subunit structurei

The acetolactate synthase complex contains the catalytic regulatory subunit ILV2 and the regulatory small subunit ILV6.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself1EBI-9087,EBI-9087
SSA2P105921EBI-9087,EBI-8603

Protein-protein interaction databases

BioGridi30972. 53 interactions.
DIPiDIP-671N.
IntActiP25605. 18 interactions.
MINTiMINT-522376.
STRINGi4932.YCL009C.

Structurei

3D structure databases

ProteinModelPortaliP25605.
SMRiP25605. Positions 77-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini79 – 15981ACTPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi25 – 306Poly-Ser

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0440.
HOGENOMiHOG000046747.
InParanoidiP25605.
KOiK01653.
OMAiGMMALPR.
OrthoDBiEOG7CCC29.

Family and domain databases

InterProiIPR004789. Acetalactate_synth_ssu.
IPR019455. Acetolactate_synth_ssu_C.
IPR002912. ACT_dom.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF10369. ALS_ss_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00119. acolac_sm. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25605-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRSLLQSGH RRVVASSCAT MVRCSSSSTS ALAYKQMHRH ATRPPLPTLD
60 70 80 90 100
TPSWNANSAV SSIIYETPAP SRQPRKQHVL NCLVQNEPGV LSRVSGTLAA
110 120 130 140 150
RGFNIDSLVV CNTEVKDLSR MTIVLQGQDG VVEQARRQIE DLVPVYAVLD
160 170 180 190 200
YTNSEIIKRE LVMARISLLG TEYFEDLLLH HHTSTNAGAA DSQELVAEIR
210 220 230 240 250
EKQFHPANLP ASEVLRLKHE HLNDITNLTN NFGGRVVDIS ETSCIVELSA
260 270 280 290 300
KPTRISAFLK LVEPFGVLEC ARSGMMALPR TPLKTSTEEA ADEDEKISEI

VDISQLPPG
Length:309
Mass (Da):33,987
Last modified:July 15, 1998 - v2
Checksum:iBF36073D89BC0065
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42350.1.
BK006937 Genomic DNA. Translation: DAA07472.1.
PIRiS19411.
RefSeqiNP_009918.1. NM_001178658.1.

Genome annotation databases

EnsemblFungiiYCL009C; YCL009C; YCL009C.
GeneIDi850348.
KEGGisce:YCL009C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42350.1 .
BK006937 Genomic DNA. Translation: DAA07472.1 .
PIRi S19411.
RefSeqi NP_009918.1. NM_001178658.1.

3D structure databases

ProteinModelPortali P25605.
SMRi P25605. Positions 77-280.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 30972. 53 interactions.
DIPi DIP-671N.
IntActi P25605. 18 interactions.
MINTi MINT-522376.
STRINGi 4932.YCL009C.

Proteomic databases

MaxQBi P25605.
PaxDbi P25605.
PeptideAtlasi P25605.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCL009C ; YCL009C ; YCL009C .
GeneIDi 850348.
KEGGi sce:YCL009C.

Organism-specific databases

CYGDi YCL009c.
SGDi S000000515. ILV6.

Phylogenomic databases

eggNOGi COG0440.
HOGENOMi HOG000046747.
InParanoidi P25605.
KOi K01653.
OMAi GMMALPR.
OrthoDBi EOG7CCC29.

Enzyme and pathway databases

UniPathwayi UPA00047 ; UER00055 .
UPA00049 ; UER00059 .
BioCyci YEAST:YCL009C-MONOMER.

Miscellaneous databases

NextBioi 965806.

Gene expression databases

Genevestigatori P25605.

Family and domain databases

InterProi IPR004789. Acetalactate_synth_ssu.
IPR019455. Acetolactate_synth_ssu_C.
IPR002912. ACT_dom.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF10369. ALS_ss_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00119. acolac_sm. 1 hit.
PROSITEi PS51671. ACT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Gromadka R.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 300.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Comprehensive sequence analysis of the 182 predicted open reading frames of yeast chromosome III."
    Bork P., Ouzounis C., Sander C., Scharf M., Schneider R., Sonnhammer E.
    Protein Sci. 1:1677-1690(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIMILARITY TO ACETOLACTATE SYNTHASE SMALL SUBUNITS.
  5. "Two-dimensional protein map of Saccharomyces cerevisiae: construction of a gene-protein index."
    Boucherie H., Dujardin G., Kermorgant M., Monribot C., Slonimski P.P., Perrot M.
    Yeast 11:601-613(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-44.
    Strain: ATCC 204508 / S288c.
  6. "Functional analysis of YCL09C: evidence for a role as the regulatory subunit of acetolactate synthase."
    Cullin C., Baudin-Baillieu A., Guillemet E., Ozier-Kalogeropoulos O.
    Yeast 12:1511-1518(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "Expression, purification, characterization, and reconstitution of the large and small subunits of yeast acetohydroxyacid synthase."
    Pang S.S., Duggleby R.G.
    Biochemistry 38:5222-5231(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, RECONSTITUTION OF THE ACETOLACTATE SYNTHASE COMPLEX.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiILV6_YEAST
AccessioniPrimary (citable) accession number: P25605
Secondary accession number(s): D6VR03, P87009
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 15, 1998
Last modified: October 29, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3