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Protein

Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease

Gene

STP22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association to the ESCRT-0 complex. Required for vacuolar targeting of temperature-sensitive plasma membrane proteins STE2 and CAN1.3 Publications

GO - Molecular functioni

  • ubiquitin binding Source: SGD

GO - Biological processi

  • ATP export Source: SGD
  • cellular protein modification process Source: InterPro
  • late endosome to vacuole transport Source: SGD
  • negative regulation of protein polyubiquitination Source: SGD
  • protein targeting to membrane Source: UniProtKB
  • protein targeting to vacuole Source: UniProtKB
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29278-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease
Alternative name(s):
ESCRT-I complex subunit VPS23
Vacuolar protein sorting-associated protein 23
Gene namesi
Name:STP22
Synonyms:VPS23
Ordered Locus Names:YCL008C
ORF Names:YCL8C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL008C.
SGDiS000000514. STP22.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic side of plasma membrane Source: SGD
  • endosome Source: UniProtKB
  • ESCRT I complex Source: SGD
  • late endosome membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi85M → T: No interaction of the ESCRT-I complex with ubiquitin. 1 Publication1
Mutagenesisi254M → D: Defective in ESCRT-I cargo sorting; reduces MVB12 localization to MVBs; abolishes interaction with MVB12; reduces interaction with SRN2. 1 Publication1
Mutagenesisi286L → D: Defective in ESCRT-I cargo sorting. 1 Publication1
Mutagenesisi345L → D: Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVBs). 1 Publication1
Mutagenesisi371F → D: Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVBs). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000826051 – 385Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permeaseAdd BLAST385

Proteomic databases

MaxQBiP25604.
PRIDEiP25604.

Interactioni

Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of STP22, VPS28, SRN2 and MVB12 in a 1:1:1:1 stoechiometry. Interacts with HSE1 and VPS27. Interactis with MVB12 and SRN2.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HOF1Q050802EBI-411625,EBI-5412
HSE1P387533EBI-411625,EBI-1382
LSB3P436033EBI-411625,EBI-22980
MVB12P429396EBI-411625,EBI-23478
PEX13P806672EBI-411625,EBI-13206
SRN2Q991765EBI-411625,EBI-18076
VPS27P403437EBI-411625,EBI-20380
VPS28Q027672EBI-411625,EBI-20387
YSC84P327932EBI-411625,EBI-24460

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi30973. 158 interactors.
DIPiDIP-5827N.
IntActiP25604. 10 interactors.
MINTiMINT-468287.

Structurei

Secondary structure

1385
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 21Combined sources11
Turni22 – 24Combined sources3
Helixi28 – 41Combined sources14
Beta strandi45 – 53Combined sources9
Beta strandi59 – 70Combined sources12
Beta strandi73 – 76Combined sources4
Beta strandi80 – 86Combined sources7
Turni89 – 93Combined sources5
Beta strandi97 – 100Combined sources4
Helixi102 – 104Combined sources3
Turni107 – 109Combined sources3
Helixi116 – 119Combined sources4
Beta strandi124 – 126Combined sources3
Helixi129 – 132Combined sources4
Helixi136 – 138Combined sources3
Helixi141 – 150Combined sources10
Helixi219 – 246Combined sources28
Helixi248 – 251Combined sources4
Helixi254 – 289Combined sources36
Helixi291 – 303Combined sources13
Helixi314 – 316Combined sources3
Helixi323 – 351Combined sources29
Helixi356 – 381Combined sources26
Turni382 – 384Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UZXX-ray1.85A1-161[»]
2CAZX-ray3.60A/D305-385[»]
2F66X-ray2.80A/D322-385[»]
2F6MX-ray2.10A/C322-385[»]
2P22X-ray2.70A215-385[»]
3R3QX-ray1.45A1-160[»]
3R42X-ray1.87A1-160[»]
ProteinModelPortaliP25604.
SMRiP25604.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25604.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 161UEVPROSITE-ProRule annotationAdd BLAST150
Domaini322 – 385SBPROSITE-ProRule annotationAdd BLAST64

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili272 – 300Sequence analysisAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi155 – 201Pro-richAdd BLAST47

Domaini

The UEV domain is required for the interaction of the complex with ubiquitin.

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.PROSITE-ProRule annotation
Contains 1 SB (steadiness box) domain.PROSITE-ProRule annotation
Contains 1 UEV (ubiquitin E2 variant) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00530000064004.
HOGENOMiHOG000057121.
InParanoidiP25604.
KOiK12183.
OMAiWHIQRIT.
OrthoDBiEOG092C36Y6.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR017916. SB_dom.
IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR008883. UEV_N.
[Graphical view]
PfamiPF05743. UEV. 1 hit.
PF09454. Vps23_core. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS51312. SB. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
PS51322. UEV. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25604-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSANGKISVP EAVVNWLFKV IQPIYNDGRT TFHDSLALLD NFHSLRPRTR
60 70 80 90 100
VFTHSDGTPQ LLLSIYGTIS TGEDGSSPHS IPVIMWVPSM YPVKPPFISI
110 120 130 140 150
NLENFDMNTI SSSLPIQEYI DSNGWIALPI LHCWDPAAMN LIMVVQELMS
160 170 180 190 200
LLHEPPQDQA PSLPPKPNTQ LQQEQNTPPL PPKPKSPHLK PPLPPPPPPQ
210 220 230 240 250
PASNALDLMD MDNTDISPTN HHEMLQNLQT VVNELYREDV DYVADKILTR
260 270 280 290 300
QTVMQESIAR FHEIIAIDKN HLRAVEQAIE QTMHSLNAQI DVLTANRAKV
310 320 330 340 350
QQFSSTSHVD DEDVNSIAVA KTDGLNQLYN LVAQDYALTD TIECLSRMLH
360 370 380
RGTIPLDTFV KQGRELARQQ FLVRWHIQRI TSPLS
Length:385
Mass (Da):43,330
Last modified:October 24, 2003 - v3
Checksum:iFEDE3BE79F063BCE
GO

Sequence cautioni

The sequence CAC42964 differs from that shown. Reason: Frameshift at position 294.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004731 Genomic DNA. Translation: AAB62820.1.
X59720 Genomic DNA. Translation: CAC42964.1. Frameshift.
AY260880 Genomic DNA. Translation: AAP21748.1.
BK006937 Genomic DNA. Translation: DAA07473.1.
PIRiS74288.
RefSeqiNP_009919.3. NM_001178657.1.

Genome annotation databases

EnsemblFungiiCAC42964; CAC42964; CAC42964.
YCL008C; YCL008C; YCL008C.
GeneIDi850349.
KEGGisce:YCL008C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004731 Genomic DNA. Translation: AAB62820.1.
X59720 Genomic DNA. Translation: CAC42964.1. Frameshift.
AY260880 Genomic DNA. Translation: AAP21748.1.
BK006937 Genomic DNA. Translation: DAA07473.1.
PIRiS74288.
RefSeqiNP_009919.3. NM_001178657.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UZXX-ray1.85A1-161[»]
2CAZX-ray3.60A/D305-385[»]
2F66X-ray2.80A/D322-385[»]
2F6MX-ray2.10A/C322-385[»]
2P22X-ray2.70A215-385[»]
3R3QX-ray1.45A1-160[»]
3R42X-ray1.87A1-160[»]
ProteinModelPortaliP25604.
SMRiP25604.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30973. 158 interactors.
DIPiDIP-5827N.
IntActiP25604. 10 interactors.
MINTiMINT-468287.

Proteomic databases

MaxQBiP25604.
PRIDEiP25604.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAC42964; CAC42964; CAC42964.
YCL008C; YCL008C; YCL008C.
GeneIDi850349.
KEGGisce:YCL008C.

Organism-specific databases

EuPathDBiFungiDB:YCL008C.
SGDiS000000514. STP22.

Phylogenomic databases

GeneTreeiENSGT00530000064004.
HOGENOMiHOG000057121.
InParanoidiP25604.
KOiK12183.
OMAiWHIQRIT.
OrthoDBiEOG092C36Y6.

Enzyme and pathway databases

BioCyciYEAST:G3O-29278-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP25604.
PROiP25604.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR017916. SB_dom.
IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR008883. UEV_N.
[Graphical view]
PfamiPF05743. UEV. 1 hit.
PF09454. Vps23_core. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS51312. SB. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
PS51322. UEV. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTP22_YEAST
AccessioniPrimary (citable) accession number: P25604
Secondary accession number(s): D6VR04
, P87010, P87279, Q86ZT3, Q8NIM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 24, 2003
Last modified: November 2, 2016
This is version 152 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1360 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.