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P25596

- GEX1_YEAST

UniProt

P25596 - GEX1_YEAST

Protein

Glutathione exchanger 1

Gene

GEX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 4 (10 Oct 2003)
      Previous versions | rss
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    Functioni

    Proton/glutathione antiporter that imports glutathione from the vacuole and exports it through the plasma membrane. Involved in resistance to oxidative stress and modulation of the PKA pathway.1 Publication

    GO - Molecular functioni

    1. solute:proton antiporter activity Source: SGD

    GO - Biological processi

    1. glutathione transmembrane transport Source: SGD
    2. hydrogen ion transmembrane transport Source: GOC

    Keywords - Biological processi

    Antiport, Ion transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29317-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione exchanger 1
    Gene namesi
    Name:GEX1
    Ordered Locus Names:YCL073C
    ORF Names:YCL70C/YCL71C/YCL73C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCL073c.
    SGDiS000000575. GEX1.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: SGD
    3. vacuolar membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 615615Glutathione exchanger 1PRO_0000202557Add
    BLAST

    Expressioni

    Inductioni

    By iron depletion and by heat.2 Publications

    Gene expression databases

    GenevestigatoriP25596.

    Interactioni

    Protein-protein interaction databases

    BioGridi30919. 2 interactions.
    DIPiDIP-8068N.
    IntActiP25596. 2 interactions.
    MINTiMINT-1355979.

    Structurei

    3D structure databases

    ProteinModelPortaliP25596.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5858CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini80 – 12041ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini142 – 15211CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini174 – 18613ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini208 – 2169CytoplasmicSequence Analysis
    Topological domaini238 – 27538ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini297 – 30610CytoplasmicSequence Analysis
    Topological domaini328 – 34316ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini365 – 38319CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini405 – 4073ExtracellularSequence Analysis
    Topological domaini429 – 44012CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini462 – 54786ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini569 – 61547CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei59 – 7921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei121 – 14121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei153 – 17321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei187 – 20721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei217 – 23721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei276 – 29621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei307 – 32721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei344 – 36421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei384 – 40421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei408 – 42821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei441 – 46121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei548 – 56821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the major facilitator superfamily.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00390000002930.
    OMAiLEHETWR.
    OrthoDBiEOG7PZS6Z.

    Family and domain databases

    InterProiIPR011701. MFS.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    P25596-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSVVGASS NKKSGIRQSC EIIERERHSN DDTYSMTSTF FKLKENEIMS    50
    AQFDSLKYKI LLISTAFVCG FGISLDYTLR STYTGYATNS YSEHSLLSTV 100
    QVINAVVSVG SQVVYSRLSD HFGRLRLFLV ATIFYIMGTI IQSQATRLTM 150
    YAAGSVFYNC GYVGTNLLLT LILSDFSSLK WRMFYQYASY WPYIIIPWIS 200
    GNIITAANPQ KNWSWNIAMW AFIYPLSALP IIFLILYMKY KSSKTAEWRS 250
    LKEQARKERT GGLFENLVFL FWKLDIVGIL LITVSLGCIL VPLTLANETS 300
    QKWHNSKIIA TLVSGGCLFF IFLYWEAKFA KSPLLPFKLL SDRGIWAPLG 350
    VTFFNFFTFF ISCDYLYPVL LVSMKESSTS AARIVNLPDF VAATASPFYS 400
    LLVAKTRKLK LSVIGGCAAW MVCMGLFYKY RGGSGSHEGV IAASVIMGLS 450
    GLLCSNSVIV ILQAMTTHSR MAVITGIQYT FSKLGAAIGA SVSGAIWTQT 500
    MPNQLYKNLG NDTLAEIAYA SPYTFISDYP WGSPERDAVV ESYRYVQRII 550
    MTVGLACTVP FFAFTMFMRD PELIDKATHE EFTEDGLVVL PDEENIFSQI 600
    KALFRHNRSN KKLGC 615
    Length:615
    Mass (Da):68,921
    Last modified:October 10, 2003 - v4
    Checksum:iDB62B9A0DFD27F12
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAC42950.1.
    BK006937 Genomic DNA. Translation: DAA07418.1.
    PIRiS74277.
    RefSeqiNP_009863.2. NM_001178711.1.

    Genome annotation databases

    EnsemblFungiiYCL073C; YCL073C; YCL073C.
    GeneIDi850289.
    KEGGisce:YCL073C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAC42950.1 .
    BK006937 Genomic DNA. Translation: DAA07418.1 .
    PIRi S74277.
    RefSeqi NP_009863.2. NM_001178711.1.

    3D structure databases

    ProteinModelPortali P25596.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30919. 2 interactions.
    DIPi DIP-8068N.
    IntActi P25596. 2 interactions.
    MINTi MINT-1355979.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCL073C ; YCL073C ; YCL073C .
    GeneIDi 850289.
    KEGGi sce:YCL073C.

    Organism-specific databases

    CYGDi YCL073c.
    SGDi S000000575. GEX1.

    Phylogenomic databases

    GeneTreei ENSGT00390000002930.
    OMAi LEHETWR.
    OrthoDBi EOG7PZS6Z.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29317-MONOMER.

    Miscellaneous databases

    NextBioi 965649.

    Gene expression databases

    Genevestigatori P25596.

    Family and domain databases

    InterProi IPR011701. MFS.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Subtelomeric duplications in Saccharomyces cerevisiae chromosomes III and XI: topology, arrangements, corrections of sequence and strain-specific polymorphism."
      Gromadka R., Gora M., Zielenkiewicz U., Slonimski P.P., Rytka J.
      Yeast 12:583-591(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
    3. Valles G., Volckaerts G.
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 605; 610 AND 612-613.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Response of genes associated with mitochondrial function to mild heat stress in yeast Saccharomyces cerevisiae."
      Sakaki K., Tashiro K., Kuhara S., Mihara K.
      J. Biochem. 134:373-384(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    7. "Gex1 is a yeast glutathione exchanger that interferes with pH and redox homeostasis."
      Dhaoui M., Auchere F., Blaiseau P.L., Lesuisse E., Landoulsi A., Camadro J.M., Haguenauer-Tsapis R., Belgareh-Touze N.
      Mol. Biol. Cell 22:2054-2067(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiGEX1_YEAST
    AccessioniPrimary (citable) accession number: P25596
    Secondary accession number(s): D6VQU9
    , P25597, P25599, P87002, Q8NIN5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: October 10, 2003
    Last modified: October 1, 2014
    This is version 89 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3