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P25596 (GEX1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione exchanger 1
Gene names
Name:GEX1
Ordered Locus Names:YCL073C
ORF Names:YCL70C/YCL71C/YCL73C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length615 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Proton/glutathione antiporter that imports glutathione from the vacuole and exports it through the plasma membrane. Involved in resistance to oxidative stress and modulation of the PKA pathway. Ref.7

Subcellular location

Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein Ref.7.

Induction

By iron depletion and by heat. Ref.5 Ref.7

Sequence similarities

Belongs to the major facilitator superfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 615615Glutathione exchanger 1
PRO_0000202557

Regions

Topological domain1 – 5858Cytoplasmic Potential
Transmembrane59 – 7921Helical; Potential
Topological domain80 – 12041Extracellular Potential
Transmembrane121 – 14121Helical; Potential
Topological domain142 – 15211Cytoplasmic Potential
Transmembrane153 – 17321Helical; Potential
Topological domain174 – 18613Extracellular Potential
Transmembrane187 – 20721Helical; Potential
Topological domain208 – 2169Cytoplasmic Potential
Transmembrane217 – 23721Helical; Potential
Topological domain238 – 27538Extracellular Potential
Transmembrane276 – 29621Helical; Potential
Topological domain297 – 30610Cytoplasmic Potential
Transmembrane307 – 32721Helical; Potential
Topological domain328 – 34316Extracellular Potential
Transmembrane344 – 36421Helical; Potential
Topological domain365 – 38319Cytoplasmic Potential
Transmembrane384 – 40421Helical; Potential
Topological domain405 – 4073Extracellular Potential
Transmembrane408 – 42821Helical; Potential
Topological domain429 – 44012Cytoplasmic Potential
Transmembrane441 – 46121Helical; Potential
Topological domain462 – 54786Extracellular Potential
Transmembrane548 – 56821Helical; Potential
Topological domain569 – 61547Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P25596 [UniParc].

Last modified October 10, 2003. Version 4.
Checksum: DB62B9A0DFD27F12

FASTA61568,921
        10         20         30         40         50         60 
MSSSVVGASS NKKSGIRQSC EIIERERHSN DDTYSMTSTF FKLKENEIMS AQFDSLKYKI 

        70         80         90        100        110        120 
LLISTAFVCG FGISLDYTLR STYTGYATNS YSEHSLLSTV QVINAVVSVG SQVVYSRLSD 

       130        140        150        160        170        180 
HFGRLRLFLV ATIFYIMGTI IQSQATRLTM YAAGSVFYNC GYVGTNLLLT LILSDFSSLK 

       190        200        210        220        230        240 
WRMFYQYASY WPYIIIPWIS GNIITAANPQ KNWSWNIAMW AFIYPLSALP IIFLILYMKY 

       250        260        270        280        290        300 
KSSKTAEWRS LKEQARKERT GGLFENLVFL FWKLDIVGIL LITVSLGCIL VPLTLANETS 

       310        320        330        340        350        360 
QKWHNSKIIA TLVSGGCLFF IFLYWEAKFA KSPLLPFKLL SDRGIWAPLG VTFFNFFTFF 

       370        380        390        400        410        420 
ISCDYLYPVL LVSMKESSTS AARIVNLPDF VAATASPFYS LLVAKTRKLK LSVIGGCAAW 

       430        440        450        460        470        480 
MVCMGLFYKY RGGSGSHEGV IAASVIMGLS GLLCSNSVIV ILQAMTTHSR MAVITGIQYT 

       490        500        510        520        530        540 
FSKLGAAIGA SVSGAIWTQT MPNQLYKNLG NDTLAEIAYA SPYTFISDYP WGSPERDAVV 

       550        560        570        580        590        600 
ESYRYVQRII MTVGLACTVP FFAFTMFMRD PELIDKATHE EFTEDGLVVL PDEENIFSQI 

       610 
KALFRHNRSN KKLGC 

« Hide

References

« Hide 'large scale' references
[1]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Subtelomeric duplications in Saccharomyces cerevisiae chromosomes III and XI: topology, arrangements, corrections of sequence and strain-specific polymorphism."
Gromadka R., Gora M., Zielenkiewicz U., Slonimski P.P., Rytka J.
Yeast 12:583-591(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[3]Valles G., Volckaerts G.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 605; 610 AND 612-613.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Response of genes associated with mitochondrial function to mild heat stress in yeast Saccharomyces cerevisiae."
Sakaki K., Tashiro K., Kuhara S., Mihara K.
J. Biochem. 134:373-384(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[7]"Gex1 is a yeast glutathione exchanger that interferes with pH and redox homeostasis."
Dhaoui M., Auchere F., Blaiseau P.L., Lesuisse E., Landoulsi A., Camadro J.M., Haguenauer-Tsapis R., Belgareh-Touze N.
Mol. Biol. Cell 22:2054-2067(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59720 Genomic DNA. Translation: CAC42950.1.
BK006937 Genomic DNA. Translation: DAA07418.1.
PIRS74277.
RefSeqNP_009863.2. NM_001178711.1.

3D structure databases

ProteinModelPortalP25596.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid30919. 2 interactions.
DIPDIP-8068N.
IntActP25596. 2 interactions.
MINTMINT-1355979.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCL073C; YCL073C; YCL073C.
GeneID850289.
KEGGsce:YCL073C.

Organism-specific databases

CYGDYCL073c.
SGDS000000575. GEX1.

Phylogenomic databases

GeneTreeENSGT00390000002930.
OMALEHETWR.
OrthoDBEOG7PZS6Z.

Enzyme and pathway databases

BioCycYEAST:G3O-29317-MONOMER.

Gene expression databases

GenevestigatorP25596.

Family and domain databases

InterProIPR011701. MFS.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 3 hits.
ProtoNetSearch...

Other

NextBio965649.

Entry information

Entry nameGEX1_YEAST
AccessionPrimary (citable) accession number: P25596
Secondary accession number(s): D6VQU9 expand/collapse secondary AC list , P25597, P25599, P87002, Q8NIN5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 10, 2003
Last modified: April 16, 2014
This is version 85 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families