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P25596

- GEX1_YEAST

UniProt

P25596 - GEX1_YEAST

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Protein

Glutathione exchanger 1

Gene

GEX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Proton/glutathione antiporter that imports glutathione from the vacuole and exports it through the plasma membrane. Involved in resistance to oxidative stress and modulation of the PKA pathway.1 Publication

GO - Molecular functioni

  1. solute:proton antiporter activity Source: SGD

GO - Biological processi

  1. glutathione transmembrane transport Source: SGD
  2. hydrogen ion transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29317-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione exchanger 1
Gene namesi
Name:GEX1
Ordered Locus Names:YCL073C
ORF Names:YCL70C/YCL71C/YCL73C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCL073c.
SGDiS000000575. GEX1.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: SGD
  3. vacuolar membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 615615Glutathione exchanger 1PRO_0000202557Add
BLAST

Expressioni

Inductioni

By iron depletion and by heat.2 Publications

Gene expression databases

GenevestigatoriP25596.

Interactioni

Protein-protein interaction databases

BioGridi30919. 2 interactions.
DIPiDIP-8068N.
IntActiP25596. 2 interactions.
MINTiMINT-1355979.

Structurei

3D structure databases

ProteinModelPortaliP25596.
SMRiP25596. Positions 185-216.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5858CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini80 – 12041ExtracellularSequence AnalysisAdd
BLAST
Topological domaini142 – 15211CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini174 – 18613ExtracellularSequence AnalysisAdd
BLAST
Topological domaini208 – 2169CytoplasmicSequence Analysis
Topological domaini238 – 27538ExtracellularSequence AnalysisAdd
BLAST
Topological domaini297 – 30610CytoplasmicSequence Analysis
Topological domaini328 – 34316ExtracellularSequence AnalysisAdd
BLAST
Topological domaini365 – 38319CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini405 – 4073ExtracellularSequence Analysis
Topological domaini429 – 44012CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini462 – 54786ExtracellularSequence AnalysisAdd
BLAST
Topological domaini569 – 61547CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei59 – 7921HelicalSequence AnalysisAdd
BLAST
Transmembranei121 – 14121HelicalSequence AnalysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence AnalysisAdd
BLAST
Transmembranei187 – 20721HelicalSequence AnalysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence AnalysisAdd
BLAST
Transmembranei276 – 29621HelicalSequence AnalysisAdd
BLAST
Transmembranei307 – 32721HelicalSequence AnalysisAdd
BLAST
Transmembranei344 – 36421HelicalSequence AnalysisAdd
BLAST
Transmembranei384 – 40421HelicalSequence AnalysisAdd
BLAST
Transmembranei408 – 42821HelicalSequence AnalysisAdd
BLAST
Transmembranei441 – 46121HelicalSequence AnalysisAdd
BLAST
Transmembranei548 – 56821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000002930.
InParanoidiP25596.
OMAiLEHETWR.
OrthoDBiEOG7PZS6Z.

Family and domain databases

InterProiIPR011701. MFS.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.

Sequencei

Sequence statusi: Complete.

P25596-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSSVVGASS NKKSGIRQSC EIIERERHSN DDTYSMTSTF FKLKENEIMS
60 70 80 90 100
AQFDSLKYKI LLISTAFVCG FGISLDYTLR STYTGYATNS YSEHSLLSTV
110 120 130 140 150
QVINAVVSVG SQVVYSRLSD HFGRLRLFLV ATIFYIMGTI IQSQATRLTM
160 170 180 190 200
YAAGSVFYNC GYVGTNLLLT LILSDFSSLK WRMFYQYASY WPYIIIPWIS
210 220 230 240 250
GNIITAANPQ KNWSWNIAMW AFIYPLSALP IIFLILYMKY KSSKTAEWRS
260 270 280 290 300
LKEQARKERT GGLFENLVFL FWKLDIVGIL LITVSLGCIL VPLTLANETS
310 320 330 340 350
QKWHNSKIIA TLVSGGCLFF IFLYWEAKFA KSPLLPFKLL SDRGIWAPLG
360 370 380 390 400
VTFFNFFTFF ISCDYLYPVL LVSMKESSTS AARIVNLPDF VAATASPFYS
410 420 430 440 450
LLVAKTRKLK LSVIGGCAAW MVCMGLFYKY RGGSGSHEGV IAASVIMGLS
460 470 480 490 500
GLLCSNSVIV ILQAMTTHSR MAVITGIQYT FSKLGAAIGA SVSGAIWTQT
510 520 530 540 550
MPNQLYKNLG NDTLAEIAYA SPYTFISDYP WGSPERDAVV ESYRYVQRII
560 570 580 590 600
MTVGLACTVP FFAFTMFMRD PELIDKATHE EFTEDGLVVL PDEENIFSQI
610
KALFRHNRSN KKLGC
Length:615
Mass (Da):68,921
Last modified:October 10, 2003 - v4
Checksum:iDB62B9A0DFD27F12
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAC42950.1.
BK006937 Genomic DNA. Translation: DAA07418.1.
PIRiS74277.
RefSeqiNP_009863.2. NM_001178711.1.

Genome annotation databases

EnsemblFungiiYCL073C; YCL073C; YCL073C.
GeneIDi850289.
KEGGisce:YCL073C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAC42950.1 .
BK006937 Genomic DNA. Translation: DAA07418.1 .
PIRi S74277.
RefSeqi NP_009863.2. NM_001178711.1.

3D structure databases

ProteinModelPortali P25596.
SMRi P25596. Positions 185-216.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 30919. 2 interactions.
DIPi DIP-8068N.
IntActi P25596. 2 interactions.
MINTi MINT-1355979.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCL073C ; YCL073C ; YCL073C .
GeneIDi 850289.
KEGGi sce:YCL073C.

Organism-specific databases

CYGDi YCL073c.
SGDi S000000575. GEX1.

Phylogenomic databases

GeneTreei ENSGT00390000002930.
InParanoidi P25596.
OMAi LEHETWR.
OrthoDBi EOG7PZS6Z.

Enzyme and pathway databases

BioCyci YEAST:G3O-29317-MONOMER.

Miscellaneous databases

NextBioi 965649.

Gene expression databases

Genevestigatori P25596.

Family and domain databases

InterProi IPR011701. MFS.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view ]
Pfami PF07690. MFS_1. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Subtelomeric duplications in Saccharomyces cerevisiae chromosomes III and XI: topology, arrangements, corrections of sequence and strain-specific polymorphism."
    Gromadka R., Gora M., Zielenkiewicz U., Slonimski P.P., Rytka J.
    Yeast 12:583-591(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. Valles G., Volckaerts G.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 605; 610 AND 612-613.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Response of genes associated with mitochondrial function to mild heat stress in yeast Saccharomyces cerevisiae."
    Sakaki K., Tashiro K., Kuhara S., Mihara K.
    J. Biochem. 134:373-384(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  7. "Gex1 is a yeast glutathione exchanger that interferes with pH and redox homeostasis."
    Dhaoui M., Auchere F., Blaiseau P.L., Lesuisse E., Landoulsi A., Camadro J.M., Haguenauer-Tsapis R., Belgareh-Touze N.
    Mol. Biol. Cell 22:2054-2067(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGEX1_YEAST
AccessioniPrimary (citable) accession number: P25596
Secondary accession number(s): D6VQU9
, P25597, P25599, P87002, Q8NIN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 10, 2003
Last modified: October 29, 2014
This is version 90 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3