SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P25596

- GEX1_YEAST

UniProt

P25596 - GEX1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Glutathione exchanger 1
Gene
GEX1, YCL073C, YCL70C/YCL71C/YCL73C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Proton/glutathione antiporter that imports glutathione from the vacuole and exports it through the plasma membrane. Involved in resistance to oxidative stress and modulation of the PKA pathway.1 Publication

GO - Molecular functioni

  1. solute:proton antiporter activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. glutathione transmembrane transport Source: SGD
  2. hydrogen ion transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29317-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione exchanger 1
Gene namesi
Name:GEX1
Ordered Locus Names:YCL073C
ORF Names:YCL70C/YCL71C/YCL73C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCL073c.
SGDiS000000575. GEX1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5858Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei59 – 7921Helical; Reviewed prediction
Add
BLAST
Topological domaini80 – 12041Extracellular Reviewed prediction
Add
BLAST
Transmembranei121 – 14121Helical; Reviewed prediction
Add
BLAST
Topological domaini142 – 15211Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei153 – 17321Helical; Reviewed prediction
Add
BLAST
Topological domaini174 – 18613Extracellular Reviewed prediction
Add
BLAST
Transmembranei187 – 20721Helical; Reviewed prediction
Add
BLAST
Topological domaini208 – 2169Cytoplasmic Reviewed prediction
Transmembranei217 – 23721Helical; Reviewed prediction
Add
BLAST
Topological domaini238 – 27538Extracellular Reviewed prediction
Add
BLAST
Transmembranei276 – 29621Helical; Reviewed prediction
Add
BLAST
Topological domaini297 – 30610Cytoplasmic Reviewed prediction
Transmembranei307 – 32721Helical; Reviewed prediction
Add
BLAST
Topological domaini328 – 34316Extracellular Reviewed prediction
Add
BLAST
Transmembranei344 – 36421Helical; Reviewed prediction
Add
BLAST
Topological domaini365 – 38319Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei384 – 40421Helical; Reviewed prediction
Add
BLAST
Topological domaini405 – 4073Extracellular Reviewed prediction
Transmembranei408 – 42821Helical; Reviewed prediction
Add
BLAST
Topological domaini429 – 44012Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei441 – 46121Helical; Reviewed prediction
Add
BLAST
Topological domaini462 – 54786Extracellular Reviewed prediction
Add
BLAST
Transmembranei548 – 56821Helical; Reviewed prediction
Add
BLAST
Topological domaini569 – 61547Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: SGD
  3. vacuolar membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 615615Glutathione exchanger 1
PRO_0000202557Add
BLAST

Expressioni

Inductioni

By iron depletion and by heat.2 Publications

Gene expression databases

GenevestigatoriP25596.

Interactioni

Protein-protein interaction databases

BioGridi30919. 2 interactions.
DIPiDIP-8068N.
IntActiP25596. 2 interactions.
MINTiMINT-1355979.

Structurei

3D structure databases

ProteinModelPortaliP25596.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000002930.
OMAiLEHETWR.
OrthoDBiEOG7PZS6Z.

Family and domain databases

InterProiIPR011701. MFS.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.

Sequencei

Sequence statusi: Complete.

P25596-1 [UniParc]FASTAAdd to Basket

« Hide

MSSSVVGASS NKKSGIRQSC EIIERERHSN DDTYSMTSTF FKLKENEIMS    50
AQFDSLKYKI LLISTAFVCG FGISLDYTLR STYTGYATNS YSEHSLLSTV 100
QVINAVVSVG SQVVYSRLSD HFGRLRLFLV ATIFYIMGTI IQSQATRLTM 150
YAAGSVFYNC GYVGTNLLLT LILSDFSSLK WRMFYQYASY WPYIIIPWIS 200
GNIITAANPQ KNWSWNIAMW AFIYPLSALP IIFLILYMKY KSSKTAEWRS 250
LKEQARKERT GGLFENLVFL FWKLDIVGIL LITVSLGCIL VPLTLANETS 300
QKWHNSKIIA TLVSGGCLFF IFLYWEAKFA KSPLLPFKLL SDRGIWAPLG 350
VTFFNFFTFF ISCDYLYPVL LVSMKESSTS AARIVNLPDF VAATASPFYS 400
LLVAKTRKLK LSVIGGCAAW MVCMGLFYKY RGGSGSHEGV IAASVIMGLS 450
GLLCSNSVIV ILQAMTTHSR MAVITGIQYT FSKLGAAIGA SVSGAIWTQT 500
MPNQLYKNLG NDTLAEIAYA SPYTFISDYP WGSPERDAVV ESYRYVQRII 550
MTVGLACTVP FFAFTMFMRD PELIDKATHE EFTEDGLVVL PDEENIFSQI 600
KALFRHNRSN KKLGC 615
Length:615
Mass (Da):68,921
Last modified:October 10, 2003 - v4
Checksum:iDB62B9A0DFD27F12
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAC42950.1.
BK006937 Genomic DNA. Translation: DAA07418.1.
PIRiS74277.
RefSeqiNP_009863.2. NM_001178711.1.

Genome annotation databases

EnsemblFungiiYCL073C; YCL073C; YCL073C.
GeneIDi850289.
KEGGisce:YCL073C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAC42950.1 .
BK006937 Genomic DNA. Translation: DAA07418.1 .
PIRi S74277.
RefSeqi NP_009863.2. NM_001178711.1.

3D structure databases

ProteinModelPortali P25596.
ModBasei Search...

Protein-protein interaction databases

BioGridi 30919. 2 interactions.
DIPi DIP-8068N.
IntActi P25596. 2 interactions.
MINTi MINT-1355979.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCL073C ; YCL073C ; YCL073C .
GeneIDi 850289.
KEGGi sce:YCL073C.

Organism-specific databases

CYGDi YCL073c.
SGDi S000000575. GEX1.

Phylogenomic databases

GeneTreei ENSGT00390000002930.
OMAi LEHETWR.
OrthoDBi EOG7PZS6Z.

Enzyme and pathway databases

BioCyci YEAST:G3O-29317-MONOMER.

Miscellaneous databases

NextBioi 965649.

Gene expression databases

Genevestigatori P25596.

Family and domain databases

InterProi IPR011701. MFS.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view ]
Pfami PF07690. MFS_1. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Subtelomeric duplications in Saccharomyces cerevisiae chromosomes III and XI: topology, arrangements, corrections of sequence and strain-specific polymorphism."
    Gromadka R., Gora M., Zielenkiewicz U., Slonimski P.P., Rytka J.
    Yeast 12:583-591(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. Valles G., Volckaerts G.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 605; 610 AND 612-613.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Response of genes associated with mitochondrial function to mild heat stress in yeast Saccharomyces cerevisiae."
    Sakaki K., Tashiro K., Kuhara S., Mihara K.
    J. Biochem. 134:373-384(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  7. "Gex1 is a yeast glutathione exchanger that interferes with pH and redox homeostasis."
    Dhaoui M., Auchere F., Blaiseau P.L., Lesuisse E., Landoulsi A., Camadro J.M., Haguenauer-Tsapis R., Belgareh-Touze N.
    Mol. Biol. Cell 22:2054-2067(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGEX1_YEAST
AccessioniPrimary (citable) accession number: P25596
Secondary accession number(s): D6VQU9
, P25597, P25599, P87002, Q8NIN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 10, 2003
Last modified: July 9, 2014
This is version 88 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi