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Protein

Glutathione exchanger 1

Gene

GEX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Proton/glutathione antiporter that imports glutathione from the vacuole and exports it through the plasma membrane. Involved in resistance to oxidative stress and modulation of the PKA pathway.1 Publication

GO - Molecular functioni

  • siderophore transmembrane transporter activity Source: GO_Central
  • solute:proton antiporter activity Source: SGD

GO - Biological processi

  • cellular iron ion homeostasis Source: GO_Central
  • glutathione transmembrane transport Source: SGD
  • siderophore transmembrane transport Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29317-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione exchanger 1
Gene namesi
Name:GEX1
Ordered Locus Names:YCL073C
ORF Names:YCL70C/YCL71C/YCL73C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL073C.
SGDiS000000575. GEX1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 58CytoplasmicSequence analysisAdd BLAST58
Transmembranei59 – 79HelicalSequence analysisAdd BLAST21
Topological domaini80 – 120ExtracellularSequence analysisAdd BLAST41
Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
Topological domaini142 – 152CytoplasmicSequence analysisAdd BLAST11
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 186ExtracellularSequence analysisAdd BLAST13
Transmembranei187 – 207HelicalSequence analysisAdd BLAST21
Topological domaini208 – 216CytoplasmicSequence analysis9
Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
Topological domaini238 – 275ExtracellularSequence analysisAdd BLAST38
Transmembranei276 – 296HelicalSequence analysisAdd BLAST21
Topological domaini297 – 306CytoplasmicSequence analysis10
Transmembranei307 – 327HelicalSequence analysisAdd BLAST21
Topological domaini328 – 343ExtracellularSequence analysisAdd BLAST16
Transmembranei344 – 364HelicalSequence analysisAdd BLAST21
Topological domaini365 – 383CytoplasmicSequence analysisAdd BLAST19
Transmembranei384 – 404HelicalSequence analysisAdd BLAST21
Topological domaini405 – 407ExtracellularSequence analysis3
Transmembranei408 – 428HelicalSequence analysisAdd BLAST21
Topological domaini429 – 440CytoplasmicSequence analysisAdd BLAST12
Transmembranei441 – 461HelicalSequence analysisAdd BLAST21
Topological domaini462 – 547ExtracellularSequence analysisAdd BLAST86
Transmembranei548 – 568HelicalSequence analysisAdd BLAST21
Topological domaini569 – 615CytoplasmicSequence analysisAdd BLAST47

GO - Cellular componenti

  • endosome Source: GO_Central
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
  • vacuolar membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002025571 – 615Glutathione exchanger 1Add BLAST615

Proteomic databases

PRIDEiP25596.

PTM databases

iPTMnetiP25596.

Expressioni

Inductioni

By iron depletion and by heat.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi30919. 3 interactors.
DIPiDIP-8068N.
IntActiP25596. 2 interactors.
MINTiMINT-1355979.

Structurei

3D structure databases

ProteinModelPortaliP25596.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000002930.
InParanoidiP25596.
OMAiLEHETWR.
OrthoDBiEOG092C2P9I.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.

Sequencei

Sequence statusi: Complete.

P25596-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSVVGASS NKKSGIRQSC EIIERERHSN DDTYSMTSTF FKLKENEIMS
60 70 80 90 100
AQFDSLKYKI LLISTAFVCG FGISLDYTLR STYTGYATNS YSEHSLLSTV
110 120 130 140 150
QVINAVVSVG SQVVYSRLSD HFGRLRLFLV ATIFYIMGTI IQSQATRLTM
160 170 180 190 200
YAAGSVFYNC GYVGTNLLLT LILSDFSSLK WRMFYQYASY WPYIIIPWIS
210 220 230 240 250
GNIITAANPQ KNWSWNIAMW AFIYPLSALP IIFLILYMKY KSSKTAEWRS
260 270 280 290 300
LKEQARKERT GGLFENLVFL FWKLDIVGIL LITVSLGCIL VPLTLANETS
310 320 330 340 350
QKWHNSKIIA TLVSGGCLFF IFLYWEAKFA KSPLLPFKLL SDRGIWAPLG
360 370 380 390 400
VTFFNFFTFF ISCDYLYPVL LVSMKESSTS AARIVNLPDF VAATASPFYS
410 420 430 440 450
LLVAKTRKLK LSVIGGCAAW MVCMGLFYKY RGGSGSHEGV IAASVIMGLS
460 470 480 490 500
GLLCSNSVIV ILQAMTTHSR MAVITGIQYT FSKLGAAIGA SVSGAIWTQT
510 520 530 540 550
MPNQLYKNLG NDTLAEIAYA SPYTFISDYP WGSPERDAVV ESYRYVQRII
560 570 580 590 600
MTVGLACTVP FFAFTMFMRD PELIDKATHE EFTEDGLVVL PDEENIFSQI
610
KALFRHNRSN KKLGC
Length:615
Mass (Da):68,921
Last modified:October 10, 2003 - v4
Checksum:iDB62B9A0DFD27F12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42950.1.
BK006937 Genomic DNA. Translation: DAA07418.1.
PIRiS74277.
RefSeqiNP_009863.2. NM_001178711.1.

Genome annotation databases

EnsemblFungiiCAC42950; CAC42950; CAC42950.
YCL073C; YCL073C; YCL073C.
GeneIDi850289.
KEGGisce:YCL073C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42950.1.
BK006937 Genomic DNA. Translation: DAA07418.1.
PIRiS74277.
RefSeqiNP_009863.2. NM_001178711.1.

3D structure databases

ProteinModelPortaliP25596.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30919. 3 interactors.
DIPiDIP-8068N.
IntActiP25596. 2 interactors.
MINTiMINT-1355979.

PTM databases

iPTMnetiP25596.

Proteomic databases

PRIDEiP25596.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAC42950; CAC42950; CAC42950.
YCL073C; YCL073C; YCL073C.
GeneIDi850289.
KEGGisce:YCL073C.

Organism-specific databases

EuPathDBiFungiDB:YCL073C.
SGDiS000000575. GEX1.

Phylogenomic databases

GeneTreeiENSGT00390000002930.
InParanoidiP25596.
OMAiLEHETWR.
OrthoDBiEOG092C2P9I.

Enzyme and pathway databases

BioCyciYEAST:G3O-29317-MONOMER.

Miscellaneous databases

PROiP25596.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGEX1_YEAST
AccessioniPrimary (citable) accession number: P25596
Secondary accession number(s): D6VQU9
, P25597, P25599, P87002, Q8NIN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 10, 2003
Last modified: November 2, 2016
This is version 107 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.