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Protein

Vacuolar basic amino acid transporter 3

Gene

VBA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transporter required for vacuolar uptake of histidine and lysine.1 Publication

GO - Molecular functioni

  • basic amino acid transmembrane transporter activity Source: SGD

GO - Biological processi

  • basic amino acid transmembrane transport Source: GOC
  • basic amino acid transport Source: SGD
  • transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29316-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar basic amino acid transporter 3
Gene namesi
Name:VBA3
Ordered Locus Names:YCL069W
ORF Names:YCL69W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL069W.
SGDiS000000574. VBA3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence analysis
Transmembranei10 – 3021HelicalSequence analysisAdd
BLAST
Topological domaini31 – 366VacuolarSequence analysis
Transmembranei37 – 5721HelicalSequence analysisAdd
BLAST
Topological domaini58 – 6710CytoplasmicSequence analysis
Transmembranei68 – 8821HelicalSequence analysisAdd
BLAST
Topological domaini89 – 13244VacuolarSequence analysisAdd
BLAST
Transmembranei133 – 15321HelicalSequence analysisAdd
BLAST
Topological domaini154 – 16310CytoplasmicSequence analysis
Transmembranei164 – 18421HelicalSequence analysisAdd
BLAST
Topological domaini185 – 20521VacuolarSequence analysisAdd
BLAST
Transmembranei206 – 22621HelicalSequence analysisAdd
BLAST
Topological domaini227 – 24822CytoplasmicSequence analysisAdd
BLAST
Transmembranei249 – 26921HelicalSequence analysisAdd
BLAST
Topological domaini270 – 2778VacuolarSequence analysis
Transmembranei278 – 29821HelicalSequence analysisAdd
BLAST
Topological domaini299 – 3068CytoplasmicSequence analysis
Transmembranei307 – 32721HelicalSequence analysisAdd
BLAST
Topological domaini328 – 41588VacuolarSequence analysisAdd
BLAST
Transmembranei416 – 43621HelicalSequence analysisAdd
BLAST
Topological domaini437 – 45822CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 458458Vacuolar basic amino acid transporter 3PRO_0000173424Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi195 – 1951N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

BioGridi30920. 12 interactions.
DIPiDIP-5032N.
IntActiP25594. 2 interactions.
MINTiMINT-543184.

Structurei

3D structure databases

ProteinModelPortaliP25594.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000247960.
InParanoidiP25594.
OMAiYVMIANI.
OrthoDBiEOG769ZTJ.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMLIVGRVV ASVGGSGLQT LCFVIGCTMV GERSRPLVIS ILSCAFAVAA
60 70 80 90 100
IVGPIIGGAF TTHVTWRWCF YINLPIGGLA IIMFLLTYKA ENKGILQQIK
110 120 130 140 150
DAIGTISSFT FSKFRHQVNF KRLMNGIIFK FDFFGFALCS AGLVLFLLGL
160 170 180 190 200
TFGGNKYSWN SGQVIAYLVL GVLLFIFSLV YDFFLFDKFN PEPDNISYRP
210 220 230 240 250
LLLRRLVAKP AIIIINMVTF LLCTGYNGQM IYSVQFFQLI FASSAWKAGL
260 270 280 290 300
HLIPIVITNV IAAIASGVIT KKLGLVKPLL IFGGVLGVIG AGLMTLMTNT
310 320 330 340 350
STKSTQIGVL LLPGFSLGFA LQASLMSAQL QITKDRPEAA MDFIEVTAFN
360 370 380 390 400
TFMKSLGTTL GGVLSTTVFS ASFHNKVSRA HLEPYEGKTV DDMILYRLQN
410 420 430 440 450
YDGSHSTIGN ILSDSIKNVF WMDLGFYALG FLFCSFSSNK KLIIPKKDET

PEDNLEDK
Length:458
Mass (Da):50,225
Last modified:May 1, 1992 - v1
Checksum:iE7B43188A5973E0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42398.1.
BK006937 Genomic DNA. Translation: DAA07419.1.
PIRiS19400.
RefSeqiNP_009864.1. NM_001178710.1.

Genome annotation databases

EnsemblFungiiCAA42398; CAA42398; CAA42398.
YCL069W; YCL069W; YCL069W.
GeneIDi850290.
KEGGisce:YCL069W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42398.1.
BK006937 Genomic DNA. Translation: DAA07419.1.
PIRiS19400.
RefSeqiNP_009864.1. NM_001178710.1.

3D structure databases

ProteinModelPortaliP25594.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30920. 12 interactions.
DIPiDIP-5032N.
IntActiP25594. 2 interactions.
MINTiMINT-543184.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42398; CAA42398; CAA42398.
YCL069W; YCL069W; YCL069W.
GeneIDi850290.
KEGGisce:YCL069W.

Organism-specific databases

EuPathDBiFungiDB:YCL069W.
SGDiS000000574. VBA3.

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000247960.
InParanoidiP25594.
OMAiYVMIANI.
OrthoDBiEOG769ZTJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-29316-MONOMER.

Miscellaneous databases

PROiP25594.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A family of basic amino acid transporters of the vacuolar membrane from Saccharomyces cerevisiae."
    Shimazu M., Sekito T., Akiyama K., Ohsumi Y., Kakinuma Y.
    J. Biol. Chem. 280:4851-4857(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiVBA3_YEAST
AccessioniPrimary (citable) accession number: P25594
Secondary accession number(s): D6VQV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 8, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.