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P25586

- KRR1_YEAST

UniProt

P25586 - KRR1_YEAST

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Protein

KRR1 small subunit processome component

Gene
KRR1, YCL059C, YCL59C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Essential for vegetative growth.4 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  2. ribosomal small subunit biogenesis Source: SGD
  3. rRNA processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29310-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
KRR1 small subunit processome component
Alternative name(s):
KRR-R motif-containing protein 1
Ribosomal RNA assembly protein KRR1
Gene namesi
Name:KRR1
Ordered Locus Names:YCL059C
ORF Names:YCL59C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCL059c.
SGDiS000000564. KRR1.

Subcellular locationi

Nucleusnucleolus 5 Publications

GO - Cellular componenti

  1. nucleolus Source: SGD
  2. small-subunit processome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi20 – 201K → E in temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with N-66; R-162 and A-261. 1 Publication
Mutagenesisi45 – 451F → L in temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with S-95 and G-207. 1 Publication
Mutagenesisi66 – 661K → N in temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; R-162 and A-261. 1 Publication
Mutagenesisi95 – 951L → S in temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with L-45 and G-207. 1 Publication
Mutagenesisi162 – 1621C → R in temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; N-66 and A-261. 1 Publication
Mutagenesisi207 – 2071R → G in temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with L-45 and S-95. 1 Publication
Mutagenesisi261 – 2611D → A in temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; N-66 and R-162. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316KRR1 small subunit processome componentPRO_0000202553Add
BLAST

Proteomic databases

MaxQBiP25586.
PaxDbiP25586.
PeptideAtlasiP25586.

Expressioni

Gene expression databases

GenevestigatoriP25586.

Interactioni

Subunit structurei

Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Interacts with snoRNA U3. Interacts with MPP10, KRI1 and with ribosomal proteins RPS1A, RPS4A, RPS4B, RPS8A, RPS8B, RPS11A, RPS11B, RPS13, RPS24, RPS25, RPL4A, RPL7B, RPL8, RPL23, RPL25 and RPL28.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KRI1P428462EBI-21773,EBI-28360

Protein-protein interaction databases

BioGridi30927. 143 interactions.
DIPiDIP-1408N.
IntActiP25586. 57 interactions.
MINTiMINT-397054.
STRINGi4932.YCL059C.

Structurei

3D structure databases

ProteinModelPortaliP25586.
SMRiP25586. Positions 42-210.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini122 – 19271KHAdd
BLAST

Sequence similaritiesi

Belongs to the KRR1 family.
Contains 1 KH domain.

Phylogenomic databases

eggNOGiCOG1094.
GeneTreeiENSGT00390000018775.
HOGENOMiHOG000116208.
KOiK06961.
OMAiRDKPWDT.
OrthoDBiEOG7DFXQ2.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR024166. rRNA_assembly_KRR1.
[Graphical view]
PANTHERiPTHR12581. PTHR12581. 1 hit.
PIRSFiPIRSF006515. KRR1. 1 hit.
SMARTiSM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.

Sequencei

Sequence statusi: Complete.

P25586-1 [UniParc]FASTAAdd to Basket

« Hide

MVSTHNRDKP WDTDDIDKWK IEEFKEEDNA SGQPFAEESS FMTLFPKYRE    50
SYLKTIWNDV TRALDKHNIA CVLDLVEGSM TVKTTRKTYD PAIILKARDL 100
IKLLARSVPF PQAVKILQDD MACDVIKIGN FVTNKERFVK RRQRLVGPNG 150
NTLKALELLT KCYILVQGNT VSAMGPFKGL KEVRRVVEDC MKNIHPIYHI 200
KELMIKRELA KRPELANEDW SRFLPMFKKR NVARKKPKKI RNVEKKVYTP 250
FPPAQLPRKV DLEIESGEYF LSKREKQMKK LNEQKEKQME REIERQEERA 300
KDFIAPEEEA YKPNQN 316
Length:316
Mass (Da):37,159
Last modified:May 1, 1992 - v1
Checksum:i7A7F964E2C7FD056
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42386.1.
AY692923 Genomic DNA. Translation: AAT92942.1.
BK006937 Genomic DNA. Translation: DAA07426.1.
PIRiS19389.
RefSeqiNP_009872.1. NM_001178703.1.

Genome annotation databases

EnsemblFungiiYCL059C; YCL059C; YCL059C.
GeneIDi850298.
KEGGisce:YCL059C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42386.1 .
AY692923 Genomic DNA. Translation: AAT92942.1 .
BK006937 Genomic DNA. Translation: DAA07426.1 .
PIRi S19389.
RefSeqi NP_009872.1. NM_001178703.1.

3D structure databases

ProteinModelPortali P25586.
SMRi P25586. Positions 42-210.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 30927. 143 interactions.
DIPi DIP-1408N.
IntActi P25586. 57 interactions.
MINTi MINT-397054.
STRINGi 4932.YCL059C.

Proteomic databases

MaxQBi P25586.
PaxDbi P25586.
PeptideAtlasi P25586.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCL059C ; YCL059C ; YCL059C .
GeneIDi 850298.
KEGGi sce:YCL059C.

Organism-specific databases

CYGDi YCL059c.
SGDi S000000564. KRR1.

Phylogenomic databases

eggNOGi COG1094.
GeneTreei ENSGT00390000018775.
HOGENOMi HOG000116208.
KOi K06961.
OMAi RDKPWDT.
OrthoDBi EOG7DFXQ2.

Enzyme and pathway databases

BioCyci YEAST:G3O-29310-MONOMER.

Miscellaneous databases

NextBioi 965673.
PROi P25586.

Gene expression databases

Genevestigatori P25586.

Family and domain databases

Gene3Di 3.30.1370.10. 1 hit.
InterProi IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR024166. rRNA_assembly_KRR1.
[Graphical view ]
PANTHERi PTHR12581. PTHR12581. 1 hit.
PIRSFi PIRSF006515. KRR1. 1 hit.
SMARTi SM00322. KH. 1 hit.
[Graphical view ]
SUPFAMi SSF54791. SSF54791. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "A novel cross-phylum family of proteins comprises a KRR1 (YCL059c) gene which is essential for viability of Saccharomyces cerevisiae cells."
    Gromadka R., Kaniak A., Slonimski P.P., Rytka J.
    Gene 171:27-32(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. "The KRR1 gene encodes a protein required for 18S rRNA synthesis and 40S ribosomal subunit assembly in Saccharomyces cerevisiae."
    Gromadka R., Rytka J.
    Acta Biochim. Pol. 47:993-1005(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Yeast Krr1p physically and functionally interacts with a novel essential Kri1p, and both proteins are required for 40S ribosome biogenesis in the nucleolus."
    Sasaki T., Toh-e A., Kikuchi Y.
    Mol. Cell. Biol. 20:7971-7979(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KRI1, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-20; PHE-45; LYS-66; LEU-95; CYS-162; ARG-207 AND ASP-261.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Functional and physical interactions of Krr1p, a Saccharomyces cerevisiae nucleolar protein."
    Gromadka R., Karkusiewicz I., Rempola B., Rytka J.
    Acta Biochim. Pol. 51:173-187(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RIBOSOMAL PROTEINS AND KRI1, SUBCELLULAR LOCATION, MUTANT KRR1-21.
  10. "The small-subunit processome is a ribosome assembly intermediate."
    Bernstein K.A., Gallagher J.E.G., Mitchell B.M., Granneman S., Baserga S.J.
    Eukaryot. Cell 3:1619-1626(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiKRR1_YEAST
AccessioniPrimary (citable) accession number: P25586
Secondary accession number(s): D6VQV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4340 molecules/cell in log phase SD medium.
Cold-sensitive mutant KRR1-21 is a deletion of amino acids 234-239.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi