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Protein

KRR1 small subunit processome component

Gene

KRR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Essential for vegetative growth.4 Publications

Miscellaneous

Present with 4340 molecules/cell in log phase SD medium.1 Publication
Cold-sensitive mutant KRR1-21 is a deletion of amino acids 234-239.

GO - Molecular functioni

GO - Biological processi

  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal small subunit biogenesis Source: SGD
  • rRNA methylation Source: Reactome
  • rRNA processing Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-29310-MONOMER
ReactomeiR-SCE-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
KRR1 small subunit processome component
Alternative name(s):
KRR-R motif-containing protein 1
Ribosomal RNA assembly protein KRR1
Gene namesi
Name:KRR1
Ordered Locus Names:YCL059C
ORF Names:YCL59C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL059C
SGDiS000000564 KRR1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi20K → E in temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with N-66; R-162 and A-261. 1 Publication1
Mutagenesisi45F → L in temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with S-95 and G-207. 1 Publication1
Mutagenesisi66K → N in temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; R-162 and A-261. 1 Publication1
Mutagenesisi95L → S in temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with L-45 and G-207. 1 Publication1
Mutagenesisi162C → R in temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; N-66 and A-261. 1 Publication1
Mutagenesisi207R → G in temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with L-45 and S-95. 1 Publication1
Mutagenesisi261D → A in temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; N-66 and R-162. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002025531 – 316KRR1 small subunit processome componentAdd BLAST316

Proteomic databases

MaxQBiP25586
PaxDbiP25586
PRIDEiP25586

Interactioni

Subunit structurei

Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Interacts with snoRNA U3. Interacts with MPP10, KRI1 and with ribosomal proteins RPS1A, RPS4A, RPS4B, RPS8A, RPS8B, RPS11A, RPS11B, RPS13, RPS24, RPS25, RPL4A, RPL7B, RPL8, RPL23, RPL25 and RPL28.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi30927, 408 interactors
DIPiDIP-1408N
IntActiP25586, 77 interactors
MINTiP25586
STRINGi4932.YCL059C

Structurei

Secondary structure

1316
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi40 – 44Combined sources5
Helixi47 – 49Combined sources3
Helixi50 – 65Combined sources16
Turni66 – 68Combined sources3
Beta strandi70 – 74Combined sources5
Turni75 – 78Combined sources4
Beta strandi79 – 83Combined sources5
Helixi93 – 105Combined sources13
Helixi110 – 113Combined sources4
Helixi114 – 117Combined sources4
Beta strandi118 – 120Combined sources3
Beta strandi122 – 127Combined sources6
Beta strandi133 – 135Combined sources3
Helixi136 – 146Combined sources11
Helixi148 – 150Combined sources3
Helixi151 – 160Combined sources10
Beta strandi163 – 167Combined sources5
Beta strandi170 – 175Combined sources6
Helixi177 – 191Combined sources15
Helixi197 – 209Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QMFX-ray2.80B/D32-222[»]
5WLCelectron microscopy3.80NK1-316[»]
5WYJelectron microscopy8.70K11-316[»]
5WYKelectron microscopy4.50K11-316[»]
ProteinModelPortaliP25586
SMRiP25586
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini122 – 192KHAdd BLAST71

Sequence similaritiesi

Belongs to the KRR1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000018775
HOGENOMiHOG000116208
InParanoidiP25586
KOiK06961
OMAiETMNNVH
OrthoDBiEOG092C3ET2

Family and domain databases

Gene3Di3.30.1370.10, 2 hits
InterProiView protein in InterPro
IPR004087 KH_dom
IPR036612 KH_dom_type_1_sf
IPR024166 rRNA_assembly_KRR1
PANTHERiPTHR12581 PTHR12581, 1 hit
PIRSFiPIRSF006515 KRR1, 1 hit
SMARTiView protein in SMART
SM00322 KH, 1 hit
SUPFAMiSSF54791 SSF54791, 1 hit

Sequencei

Sequence statusi: Complete.

P25586-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSTHNRDKP WDTDDIDKWK IEEFKEEDNA SGQPFAEESS FMTLFPKYRE
60 70 80 90 100
SYLKTIWNDV TRALDKHNIA CVLDLVEGSM TVKTTRKTYD PAIILKARDL
110 120 130 140 150
IKLLARSVPF PQAVKILQDD MACDVIKIGN FVTNKERFVK RRQRLVGPNG
160 170 180 190 200
NTLKALELLT KCYILVQGNT VSAMGPFKGL KEVRRVVEDC MKNIHPIYHI
210 220 230 240 250
KELMIKRELA KRPELANEDW SRFLPMFKKR NVARKKPKKI RNVEKKVYTP
260 270 280 290 300
FPPAQLPRKV DLEIESGEYF LSKREKQMKK LNEQKEKQME REIERQEERA
310
KDFIAPEEEA YKPNQN
Length:316
Mass (Da):37,159
Last modified:May 1, 1992 - v1
Checksum:i7A7F964E2C7FD056
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA Translation: CAA42386.1
AY692923 Genomic DNA Translation: AAT92942.1
BK006937 Genomic DNA Translation: DAA07426.1
PIRiS19389
RefSeqiNP_009872.1, NM_001178703.1

Genome annotation databases

EnsemblFungiiCAA42386; CAA42386; CAA42386
YCL059C; YCL059C; YCL059C
GeneIDi850298
KEGGisce:YCL059C

Similar proteinsi

Entry informationi

Entry nameiKRR1_YEAST
AccessioniPrimary (citable) accession number: P25586
Secondary accession number(s): D6VQV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

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