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P25586

- KRR1_YEAST

UniProt

P25586 - KRR1_YEAST

Protein

KRR1 small subunit processome component

Gene

KRR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Essential for vegetative growth.4 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    2. ribosomal small subunit biogenesis Source: SGD
    3. rRNA processing Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29310-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    KRR1 small subunit processome component
    Alternative name(s):
    KRR-R motif-containing protein 1
    Ribosomal RNA assembly protein KRR1
    Gene namesi
    Name:KRR1
    Ordered Locus Names:YCL059C
    ORF Names:YCL59C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCL059c.
    SGDiS000000564. KRR1.

    Subcellular locationi

    Nucleusnucleolus 5 Publications

    GO - Cellular componenti

    1. nucleolus Source: SGD
    2. small-subunit processome Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi20 – 201K → E in temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with N-66; R-162 and A-261. 1 Publication
    Mutagenesisi45 – 451F → L in temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with S-95 and G-207. 1 Publication
    Mutagenesisi66 – 661K → N in temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; R-162 and A-261. 1 Publication
    Mutagenesisi95 – 951L → S in temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with L-45 and G-207. 1 Publication
    Mutagenesisi162 – 1621C → R in temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; N-66 and A-261. 1 Publication
    Mutagenesisi207 – 2071R → G in temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with L-45 and S-95. 1 Publication
    Mutagenesisi261 – 2611D → A in temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; N-66 and R-162. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 316316KRR1 small subunit processome componentPRO_0000202553Add
    BLAST

    Proteomic databases

    MaxQBiP25586.
    PaxDbiP25586.
    PeptideAtlasiP25586.

    Expressioni

    Gene expression databases

    GenevestigatoriP25586.

    Interactioni

    Subunit structurei

    Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Interacts with snoRNA U3. Interacts with MPP10, KRI1 and with ribosomal proteins RPS1A, RPS4A, RPS4B, RPS8A, RPS8B, RPS11A, RPS11B, RPS13, RPS24, RPS25, RPL4A, RPL7B, RPL8, RPL23, RPL25 and RPL28.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    KRI1P428462EBI-21773,EBI-28360

    Protein-protein interaction databases

    BioGridi30927. 143 interactions.
    DIPiDIP-1408N.
    IntActiP25586. 57 interactions.
    MINTiMINT-397054.
    STRINGi4932.YCL059C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25586.
    SMRiP25586. Positions 42-210.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini122 – 19271KHAdd
    BLAST

    Sequence similaritiesi

    Belongs to the KRR1 family.Curated
    Contains 1 KH domain.Curated

    Phylogenomic databases

    eggNOGiCOG1094.
    GeneTreeiENSGT00390000018775.
    HOGENOMiHOG000116208.
    KOiK06961.
    OMAiRDKPWDT.
    OrthoDBiEOG7DFXQ2.

    Family and domain databases

    Gene3Di3.30.1370.10. 1 hit.
    InterProiIPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR024166. rRNA_assembly_KRR1.
    [Graphical view]
    PANTHERiPTHR12581. PTHR12581. 1 hit.
    PIRSFiPIRSF006515. KRR1. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    [Graphical view]
    SUPFAMiSSF54791. SSF54791. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P25586-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSTHNRDKP WDTDDIDKWK IEEFKEEDNA SGQPFAEESS FMTLFPKYRE    50
    SYLKTIWNDV TRALDKHNIA CVLDLVEGSM TVKTTRKTYD PAIILKARDL 100
    IKLLARSVPF PQAVKILQDD MACDVIKIGN FVTNKERFVK RRQRLVGPNG 150
    NTLKALELLT KCYILVQGNT VSAMGPFKGL KEVRRVVEDC MKNIHPIYHI 200
    KELMIKRELA KRPELANEDW SRFLPMFKKR NVARKKPKKI RNVEKKVYTP 250
    FPPAQLPRKV DLEIESGEYF LSKREKQMKK LNEQKEKQME REIERQEERA 300
    KDFIAPEEEA YKPNQN 316
    Length:316
    Mass (Da):37,159
    Last modified:May 1, 1992 - v1
    Checksum:i7A7F964E2C7FD056
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42386.1.
    AY692923 Genomic DNA. Translation: AAT92942.1.
    BK006937 Genomic DNA. Translation: DAA07426.1.
    PIRiS19389.
    RefSeqiNP_009872.1. NM_001178703.1.

    Genome annotation databases

    EnsemblFungiiYCL059C; YCL059C; YCL059C.
    GeneIDi850298.
    KEGGisce:YCL059C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42386.1 .
    AY692923 Genomic DNA. Translation: AAT92942.1 .
    BK006937 Genomic DNA. Translation: DAA07426.1 .
    PIRi S19389.
    RefSeqi NP_009872.1. NM_001178703.1.

    3D structure databases

    ProteinModelPortali P25586.
    SMRi P25586. Positions 42-210.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30927. 143 interactions.
    DIPi DIP-1408N.
    IntActi P25586. 57 interactions.
    MINTi MINT-397054.
    STRINGi 4932.YCL059C.

    Proteomic databases

    MaxQBi P25586.
    PaxDbi P25586.
    PeptideAtlasi P25586.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCL059C ; YCL059C ; YCL059C .
    GeneIDi 850298.
    KEGGi sce:YCL059C.

    Organism-specific databases

    CYGDi YCL059c.
    SGDi S000000564. KRR1.

    Phylogenomic databases

    eggNOGi COG1094.
    GeneTreei ENSGT00390000018775.
    HOGENOMi HOG000116208.
    KOi K06961.
    OMAi RDKPWDT.
    OrthoDBi EOG7DFXQ2.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29310-MONOMER.

    Miscellaneous databases

    NextBioi 965673.
    PROi P25586.

    Gene expression databases

    Genevestigatori P25586.

    Family and domain databases

    Gene3Di 3.30.1370.10. 1 hit.
    InterProi IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR024166. rRNA_assembly_KRR1.
    [Graphical view ]
    PANTHERi PTHR12581. PTHR12581. 1 hit.
    PIRSFi PIRSF006515. KRR1. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54791. SSF54791. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "A novel cross-phylum family of proteins comprises a KRR1 (YCL059c) gene which is essential for viability of Saccharomyces cerevisiae cells."
      Gromadka R., Kaniak A., Slonimski P.P., Rytka J.
      Gene 171:27-32(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    5. "The KRR1 gene encodes a protein required for 18S rRNA synthesis and 40S ribosomal subunit assembly in Saccharomyces cerevisiae."
      Gromadka R., Rytka J.
      Acta Biochim. Pol. 47:993-1005(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "Yeast Krr1p physically and functionally interacts with a novel essential Kri1p, and both proteins are required for 40S ribosome biogenesis in the nucleolus."
      Sasaki T., Toh-e A., Kikuchi Y.
      Mol. Cell. Biol. 20:7971-7979(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH KRI1, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-20; PHE-45; LYS-66; LEU-95; CYS-162; ARG-207 AND ASP-261.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Functional and physical interactions of Krr1p, a Saccharomyces cerevisiae nucleolar protein."
      Gromadka R., Karkusiewicz I., Rempola B., Rytka J.
      Acta Biochim. Pol. 51:173-187(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RIBOSOMAL PROTEINS AND KRI1, SUBCELLULAR LOCATION, MUTANT KRR1-21.
    10. "The small-subunit processome is a ribosome assembly intermediate."
      Bernstein K.A., Gallagher J.E.G., Mitchell B.M., Granneman S., Baserga S.J.
      Eukaryot. Cell 3:1619-1626(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiKRR1_YEAST
    AccessioniPrimary (citable) accession number: P25586
    Secondary accession number(s): D6VQV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4340 molecules/cell in log phase SD medium.1 Publication
    Cold-sensitive mutant KRR1-21 is a deletion of amino acids 234-239.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3