Reviewed, UniProtKB/Swiss-Prot P25583 (KAR4_YEAST)

Names and origin

Protein names

Recommended name:
    Karyogamy protein KAR4

Gene names

Name:	KAR4
Ordered Locus Names:	YCL055W
ORF Names:	YCL55W, YCL432

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May assist STE12 in the pheromone-dependent expression of KAR3 and CIK1. Also required for meiosis.
Subcellular location	Nucleus.
Miscellaneous	Present with 1690 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the MT-A70-like family.

Ontologies

Keywords
Biological process	Karyogamy Meiosis
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G1 phase of mitotic cell cycle Ref.3 Inferred from expression pattern. Source: SGD karyogamy during conjugation with cellular fusion Ref.3 Inferred from mutant phenotype. Source: SGD meiosis Ref.3 Inferred from mutant phenotype. Source: SGD negative regulation of transcription from RNA polymerase II promoter by pheromones Inferred from expression pattern. Source: SGD nucleobase, nucleoside, nucleotide and nucleic acid metabolic process Inferred from electronic annotation. Source: InterPro positive regulation of transcription from RNA polymerase II promoter by pheromones Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct transcription factor activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAF40	P53829	1	EBI-9509,EBI-28306
IME4	P41833	1	EBI-9509,EBI-9210
MTC2	P53185	1	EBI-9509,EBI-11537

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Karyogamy protein KAR4		PRO_0000207635
Amino acid modifications
	Modified residue	292	1	Phosphoserine Ref.5
	Modified residue	301	1	Phosphoserine Ref.5
	Modified residue	329	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P25583-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: B54A4E2E6563EC81	FASTA	335	38,672
	10 20 30 40 50 60 MAFQDPTYDQ NKSRHINNSH LQGPNQETIE MKSKHVSFKP SRDFHTNDYS NNYIHGKSLP 70 80 90 100 110 120 QQHVTNIENR VDGYPKLQKL FQAKAKQINQ FATTPFGCKI GIDSIVPTLN HWIQNENLTF 130 140 150 160 170 180 DVVMIGCLTE NQFIYPILTQ LPLDRLISKP GFLFIWANSQ KINELTKLLN NEIWAKKFRR 190 200 210 220 230 240 SEELVFVPID KKSPFYPGLD QDDETLMEKM QWHCWMCITG TVRRSTDGHL IHCNVDTDLS 250 260 270 280 290 300 IETKDTTNGA VPSHLYRIAE NFSTATRRLH IIPARTGYET PVKVRPGWVI VSPDVMLDNF 310 320 330 SPKRYKEEIA NLGSNIPLKN EIELLRPRSP VQKAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of D10B, a BamHI fragment on the small-ring chromosome III of Saccharomyces cerevisiae." Defoor E., Debrabandere R., Keyers B., Voet M., Volckaert G. Yeast 8:681-687(1992) [PubMed: 1441748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed: 1574125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Kar4p, a karyogamy-specific component of the yeast pheromone response pathway." Kurihara L.J., Stewart B.G., Gammie A.E., Rose M.D. Mol. Cell. Biol. 16:3990-4002(1996) [PubMed: 8754797] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-292; SER-301 AND SER-329, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59720 Genomic DNA. Translation: CAA42390.1.
PIR	S19385.
RefSeq	NP_009876.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1401N.
IntAct	P25583. 7 interactions.
STRING	P25583.
Proteomic databases
PeptideAtlas	P25583.
PRIDE	P25583.
Genome annotation databases
Ensembl	YCL055W; YCL055W; YCL055W; Saccharomyces cerevisiae. [Genome view]
GeneID	850303.
KEGG	sce:YCL055W.
NMPDR	fig|4932.3.peg.596.
Organism-specific databases
CYGD	YCL055w.
SGD	S000000560. KAR4.
Phylogenomic databases
HOGENOM	P25583.
OMA	CITGTVR
OrthoDB	EOG9576PX
Gene expression databases
ArrayExpress	P25583.
Genevestigator	P25583.
GermOnline	YCL055W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007757. MT-A70. [Graphical view]
Pfam	PF05063. MT-A70. 1 hit. [Graphical view]
PROSITE	PS51143. MT_A70. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	965685.

Entry information

Entry name

KAR4_YEAST

Accession

Primary (citable) accession number: P25583

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	November 24, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)