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Protein

Karyogamy protein KAR4

Gene

KAR4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May assist STE12 in the pheromone-dependent expression of KAR3 and CIK1. Also required for meiosis.

GO - Molecular functioni

GO - Biological processi

  • karyogamy involved in conjugation with cellular fusion Source: SGD
  • meiotic nuclear division Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter by pheromones Source: SGD
  • nucleobase-containing compound metabolic process Source: InterPro
  • positive regulation of transcription from RNA polymerase II promoter by pheromones Source: SGD
Complete GO annotation...

Keywords - Biological processi

Karyogamy, Meiosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29307-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Karyogamy protein KAR4
Gene namesi
Name:KAR4
Ordered Locus Names:YCL055W
ORF Names:YCL432, YCL55W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome III

Organism-specific databases

CYGDiYCL055w.
EuPathDBiFungiDB:YCL055W.
SGDiS000000560. KAR4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Karyogamy protein KAR4PRO_0000207635Add
BLAST

Proteomic databases

MaxQBiP25583.
PaxDbiP25583.
PeptideAtlasiP25583.

Expressioni

Gene expression databases

GenevestigatoriP25583.

Interactioni

Protein-protein interaction databases

BioGridi30931. 31 interactions.
DIPiDIP-1401N.
IntActiP25583. 5 interactions.
MINTiMINT-399602.
STRINGi4932.YCL055W.

Structurei

3D structure databases

ProteinModelPortaliP25583.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MT-A70-like family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG297307.
GeneTreeiENSGT00550000075003.
HOGENOMiHOG000247875.
InParanoidiP25583.
OMAiTRRLHII.
OrthoDBiEOG7NSBBS.

Family and domain databases

InterProiIPR007757. MT-A70-like.
[Graphical view]
PfamiPF05063. MT-A70. 1 hit.
[Graphical view]
PROSITEiPS51143. MT_A70. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFQDPTYDQ NKSRHINNSH LQGPNQETIE MKSKHVSFKP SRDFHTNDYS
60 70 80 90 100
NNYIHGKSLP QQHVTNIENR VDGYPKLQKL FQAKAKQINQ FATTPFGCKI
110 120 130 140 150
GIDSIVPTLN HWIQNENLTF DVVMIGCLTE NQFIYPILTQ LPLDRLISKP
160 170 180 190 200
GFLFIWANSQ KINELTKLLN NEIWAKKFRR SEELVFVPID KKSPFYPGLD
210 220 230 240 250
QDDETLMEKM QWHCWMCITG TVRRSTDGHL IHCNVDTDLS IETKDTTNGA
260 270 280 290 300
VPSHLYRIAE NFSTATRRLH IIPARTGYET PVKVRPGWVI VSPDVMLDNF
310 320 330
SPKRYKEEIA NLGSNIPLKN EIELLRPRSP VQKAQ
Length:335
Mass (Da):38,672
Last modified:May 1, 1992 - v1
Checksum:iB54A4E2E6563EC81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42390.1.
BK006937 Genomic DNA. Translation: DAA07431.1.
PIRiS19385.
RefSeqiNP_009876.1. NM_001178699.1.

Genome annotation databases

EnsemblFungiiYCL055W; YCL055W; YCL055W.
GeneIDi850303.
KEGGisce:YCL055W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42390.1.
BK006937 Genomic DNA. Translation: DAA07431.1.
PIRiS19385.
RefSeqiNP_009876.1. NM_001178699.1.

3D structure databases

ProteinModelPortaliP25583.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30931. 31 interactions.
DIPiDIP-1401N.
IntActiP25583. 5 interactions.
MINTiMINT-399602.
STRINGi4932.YCL055W.

Proteomic databases

MaxQBiP25583.
PaxDbiP25583.
PeptideAtlasiP25583.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYCL055W; YCL055W; YCL055W.
GeneIDi850303.
KEGGisce:YCL055W.

Organism-specific databases

CYGDiYCL055w.
EuPathDBiFungiDB:YCL055W.
SGDiS000000560. KAR4.

Phylogenomic databases

eggNOGiNOG297307.
GeneTreeiENSGT00550000075003.
HOGENOMiHOG000247875.
InParanoidiP25583.
OMAiTRRLHII.
OrthoDBiEOG7NSBBS.

Enzyme and pathway databases

BioCyciYEAST:G3O-29307-MONOMER.

Miscellaneous databases

NextBioi965685.
PROiP25583.

Gene expression databases

GenevestigatoriP25583.

Family and domain databases

InterProiIPR007757. MT-A70-like.
[Graphical view]
PfamiPF05063. MT-A70. 1 hit.
[Graphical view]
PROSITEiPS51143. MT_A70. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of D10B, a BamHI fragment on the small-ring chromosome III of Saccharomyces cerevisiae."
    Defoor E., Debrabandere R., Keyers B., Voet M., Volckaert G.
    Yeast 8:681-687(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Kar4p, a karyogamy-specific component of the yeast pheromone response pathway."
    Kurihara L.J., Stewart B.G., Gammie A.E., Rose M.D.
    Mol. Cell. Biol. 16:3990-4002(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKAR4_YEAST
AccessioniPrimary (citable) accession number: P25583
Secondary accession number(s): D6VQW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 29, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1690 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.