P25580 (PBN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein PBN1

Alternative name(s):
Protease B non-derepressible protein 1

Gene names

Name:	PBN1
Ordered Locus Names:	YCL052C
ORF Names:	YCL52C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for proper folding and/or the stability of a subset of proteins in the endoplasmic reticulum. Aids the autocatalytic processing of PRB1. Component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase GPI14. Ref.5 Ref.7 Ref.8
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type III membrane protein Ref.5.
Post-translational modification	N-glycosylated. Ref.5
Miscellaneous	Present with 4930 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the PIGX family.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER-associated protein catabolic process Inferred from mutant phenotype Ref.8. Source: SGD GPI anchor biosynthetic process Inferred from mutant phenotype Ref.7. Source: SGD protein processing Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.5. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Protein PBN1		PRO_0000058243
Regions
	Topological domain	1 – 385	385	Lumenal Potential
	Transmembrane	386 – 405	20	Helical; Signal-anchor for type III membrane protein; Potential
	Topological domain	406 – 416	11	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	120	1	N-linked (GlcNAc...) Potential
	Glycosylation	212	1	N-linked (GlcNAc...) Potential
	Glycosylation	365	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P25580 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 65F7B920F0C2BBF0	FASTA	416	47,923
	10 20 30 40 50 60 MVTRHRVTVL YNAPEDIGNH MRQNDTHLTV RGGSGVVLQQ RWLLERTGSL DKSFTRITWR 70 80 90 100 110 120 PRADLARSLS VIENELSAGF SVYSNSSDVP ERFITNPVYN SFHSEKFDIE QYLPPEVDLN 130 140 150 160 170 180 LSWNPEDFTY DISVEPTQIQ IVEYRLLKQG EEFTIARVKD EKLEVGVFFV DASDESDVDI 190 200 210 220 230 240 GGIRCNWRMD DGKMERCQKT SLLYKQGHIA YNHSTTTTSL YLNEPIGLHP KIMIDLTDFE 250 260 270 280 290 300 ERPKCMYLMH LQLPLELFID KFQSSPLLLF GEDDLELPEY SLRDKAWGSE SIFELKAGTM 310 320 330 340 350 360 NEVTLHTRYI EPSNNKGDKL EVSFDPEVIL ACDTGDNKVS RNPFYKKGLG YESLFTDDTT 370 380 390 400 410 FRHLNSTTLL VPIPRPDTKD YSKIKNGTLL CLLISIIYIF SKVFGNNKKK RSVKRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Multiple copies of PBS2, MHP1 or LRE1 produce glucanase resistance and other cell wall effects in Saccharomyces cerevisiae." Lai M.H., Silverman S.J., Gaughran J.P., Kirsch D.R. Yeast 13:199-213(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"The PBN1 gene of Saccharomyces cerevisiae: an essential gene that is required for the post-translational processing of the protease B precursor." Naik R.R., Jones E.W. Genetics 149:1277-1292(1998) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, FUNCTION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Mammalian PIG-X and yeast Pbn1p are the essential components of glycosylphosphatidylinositol-mannosyltransferase I." Ashida H., Hong Y., Murakami Y., Shishioh N., Sugimoto N., Kim Y.U., Maeda Y., Kinoshita T. Mol. Biol. Cell 16:1439-1448(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Pbn1p: an essential endoplasmic reticulum membrane protein required for protein processing in the endoplasmic reticulum of budding yeast." Subramanian S., Woolford C.A., Drill E., Lu M., Jones E.W. Proc. Natl. Acad. Sci. U.S.A. 103:939-944(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59720 Genomic DNA. Translation: CAA42392.1. AY692756 Genomic DNA. Translation: AAT92775.1. BK006937 Genomic DNA. Translation: DAA07433.1.
PIR	S19382.
RefSeq	NP_009878.1. NM_001178697.1.
3D structure databases
ProteinModelPortal	P25580.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8257N.
MINT	MINT-1355074.
STRING	4932.YCL052C.
Proteomic databases
PaxDb	P25580.
PeptideAtlas	P25580.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCL052C; YCL052C; YCL052C.
GeneID	850305.
KEGG	sce:YCL052C.
Organism-specific databases
CYGD	YCL052c.
SGD	S000000557. PBN1.
Phylogenomic databases
eggNOG	NOG86258.
HOGENOM	HOG000115465.
KO	K07541.
OMA	SGLRCTW.
OrthoDB	EOG42RHHD.
Enzyme and pathway databases
BRENDA	3.4.21.48. 984.
UniPathway	UPA00196.
Gene expression databases
Genevestigator	P25580.
GermOnline	YCL052C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013233. PIG-X/PBN1. [Graphical view]
Pfam	PF08320. PIG-X. 1 hit. [Graphical view]
SMART	SM00780. PIG-X. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	965691.

Entry information

Entry name

PBN1_YEAST

Accession

Primary (citable) accession number: P25580
Secondary accession number(s): D6VQW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program