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P25580

- PBN1_YEAST

UniProt

P25580 - PBN1_YEAST

Protein

Protein PBN1

Gene

PBN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Required for proper folding and/or the stability of a subset of proteins in the endoplasmic reticulum. Aids the autocatalytic processing of PRB1. Component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase GPI14.3 Publications

    Pathwayi

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
    2. GPI anchor biosynthetic process Source: SGD
    3. mannosylation Source: GOC
    4. protein processing Source: SGD

    Keywords - Biological processi

    GPI-anchor biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29306-MONOMER.
    BRENDAi3.4.21.48. 984.
    UniPathwayiUPA00196.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein PBN1
    Alternative name(s):
    Protease B non-derepressible protein 1
    Gene namesi
    Name:PBN1
    Ordered Locus Names:YCL052C
    ORF Names:YCL52C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCL052c.
    SGDiS000000557. PBN1.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Single-pass type III membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 416416Protein PBN1PRO_0000058243Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi212 – 2121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP25580.
    PaxDbiP25580.
    PeptideAtlasiP25580.

    Expressioni

    Gene expression databases

    GenevestigatoriP25580.

    Interactioni

    Protein-protein interaction databases

    BioGridi30933. 31 interactions.
    DIPiDIP-8257N.
    IntActiP25580. 5 interactions.
    MINTiMINT-1355074.
    STRINGi4932.YCL052C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25580.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 385385LumenalSequence AnalysisAdd
    BLAST
    Topological domaini406 – 41611CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei386 – 40520Helical; Signal-anchor for type III membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PIGX family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG86258.
    HOGENOMiHOG000115465.
    KOiK07541.
    OMAiSGLRCTW.
    OrthoDBiEOG783N68.

    Family and domain databases

    InterProiIPR013233. PIG-X/PBN1.
    [Graphical view]
    PfamiPF08320. PIG-X. 1 hit.
    [Graphical view]
    SMARTiSM00780. PIG-X. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25580-1 [UniParc]FASTAAdd to Basket

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    MVTRHRVTVL YNAPEDIGNH MRQNDTHLTV RGGSGVVLQQ RWLLERTGSL    50
    DKSFTRITWR PRADLARSLS VIENELSAGF SVYSNSSDVP ERFITNPVYN 100
    SFHSEKFDIE QYLPPEVDLN LSWNPEDFTY DISVEPTQIQ IVEYRLLKQG 150
    EEFTIARVKD EKLEVGVFFV DASDESDVDI GGIRCNWRMD DGKMERCQKT 200
    SLLYKQGHIA YNHSTTTTSL YLNEPIGLHP KIMIDLTDFE ERPKCMYLMH 250
    LQLPLELFID KFQSSPLLLF GEDDLELPEY SLRDKAWGSE SIFELKAGTM 300
    NEVTLHTRYI EPSNNKGDKL EVSFDPEVIL ACDTGDNKVS RNPFYKKGLG 350
    YESLFTDDTT FRHLNSTTLL VPIPRPDTKD YSKIKNGTLL CLLISIIYIF 400
    SKVFGNNKKK RSVKRE 416
    Length:416
    Mass (Da):47,923
    Last modified:May 1, 1992 - v1
    Checksum:i65F7B920F0C2BBF0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42392.1.
    AY692756 Genomic DNA. Translation: AAT92775.1.
    BK006937 Genomic DNA. Translation: DAA07433.1.
    PIRiS19382.
    RefSeqiNP_009878.1. NM_001178697.1.

    Genome annotation databases

    EnsemblFungiiYCL052C; YCL052C; YCL052C.
    GeneIDi850305.
    KEGGisce:YCL052C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42392.1 .
    AY692756 Genomic DNA. Translation: AAT92775.1 .
    BK006937 Genomic DNA. Translation: DAA07433.1 .
    PIRi S19382.
    RefSeqi NP_009878.1. NM_001178697.1.

    3D structure databases

    ProteinModelPortali P25580.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30933. 31 interactions.
    DIPi DIP-8257N.
    IntActi P25580. 5 interactions.
    MINTi MINT-1355074.
    STRINGi 4932.YCL052C.

    Proteomic databases

    MaxQBi P25580.
    PaxDbi P25580.
    PeptideAtlasi P25580.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCL052C ; YCL052C ; YCL052C .
    GeneIDi 850305.
    KEGGi sce:YCL052C.

    Organism-specific databases

    CYGDi YCL052c.
    SGDi S000000557. PBN1.

    Phylogenomic databases

    eggNOGi NOG86258.
    HOGENOMi HOG000115465.
    KOi K07541.
    OMAi SGLRCTW.
    OrthoDBi EOG783N68.

    Enzyme and pathway databases

    UniPathwayi UPA00196 .
    BioCyci YEAST:G3O-29306-MONOMER.
    BRENDAi 3.4.21.48. 984.

    Miscellaneous databases

    NextBioi 965691.

    Gene expression databases

    Genevestigatori P25580.

    Family and domain databases

    InterProi IPR013233. PIG-X/PBN1.
    [Graphical view ]
    Pfami PF08320. PIG-X. 1 hit.
    [Graphical view ]
    SMARTi SM00780. PIG-X. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Multiple copies of PBS2, MHP1 or LRE1 produce glucanase resistance and other cell wall effects in Saccharomyces cerevisiae."
      Lai M.H., Silverman S.J., Gaughran J.P., Kirsch D.R.
      Yeast 13:199-213(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "The PBN1 gene of Saccharomyces cerevisiae: an essential gene that is required for the post-translational processing of the protease B precursor."
      Naik R.R., Jones E.W.
      Genetics 149:1277-1292(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, FUNCTION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Mammalian PIG-X and yeast Pbn1p are the essential components of glycosylphosphatidylinositol-mannosyltransferase I."
      Ashida H., Hong Y., Murakami Y., Shishioh N., Sugimoto N., Kim Y.U., Maeda Y., Kinoshita T.
      Mol. Biol. Cell 16:1439-1448(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Pbn1p: an essential endoplasmic reticulum membrane protein required for protein processing in the endoplasmic reticulum of budding yeast."
      Subramanian S., Woolford C.A., Drill E., Lu M., Jones E.W.
      Proc. Natl. Acad. Sci. U.S.A. 103:939-944(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPBN1_YEAST
    AccessioniPrimary (citable) accession number: P25580
    Secondary accession number(s): D6VQW4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4930 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3