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P25580

- PBN1_YEAST

UniProt

P25580 - PBN1_YEAST

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Protein

Protein PBN1

Gene
PBN1, YCL052C, YCL52C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Required for proper folding and/or the stability of a subset of proteins in the endoplasmic reticulum. Aids the autocatalytic processing of PRB1. Component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase GPI14.3 Publications

Pathwayi

GO - Biological processi

  1. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  2. GPI anchor biosynthetic process Source: SGD
  3. mannosylation Source: GOC
  4. protein processing Source: SGD
Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-29306-MONOMER.
BRENDAi3.4.21.48. 984.
UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PBN1
Alternative name(s):
Protease B non-derepressible protein 1
Gene namesi
Name:PBN1
Ordered Locus Names:YCL052C
ORF Names:YCL52C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCL052c.
SGDiS000000557. PBN1.

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass type III membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 385385Lumenal Reviewed predictionAdd
BLAST
Transmembranei386 – 40520Helical; Signal-anchor for type III membrane protein; Reviewed predictionAdd
BLAST
Topological domaini406 – 41611Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416Protein PBN1PRO_0000058243Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...) Reviewed prediction
Glycosylationi85 – 851N-linked (GlcNAc...) Reviewed prediction
Glycosylationi120 – 1201N-linked (GlcNAc...) Reviewed prediction
Glycosylationi212 – 2121N-linked (GlcNAc...) Reviewed prediction
Glycosylationi365 – 3651N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP25580.
PaxDbiP25580.
PeptideAtlasiP25580.

Expressioni

Gene expression databases

GenevestigatoriP25580.

Interactioni

Protein-protein interaction databases

BioGridi30933. 31 interactions.
DIPiDIP-8257N.
IntActiP25580. 5 interactions.
MINTiMINT-1355074.
STRINGi4932.YCL052C.

Structurei

3D structure databases

ProteinModelPortaliP25580.

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGX family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG86258.
HOGENOMiHOG000115465.
KOiK07541.
OMAiSGLRCTW.
OrthoDBiEOG783N68.

Family and domain databases

InterProiIPR013233. PIG-X/PBN1.
[Graphical view]
PfamiPF08320. PIG-X. 1 hit.
[Graphical view]
SMARTiSM00780. PIG-X. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25580-1 [UniParc]FASTAAdd to Basket

« Hide

MVTRHRVTVL YNAPEDIGNH MRQNDTHLTV RGGSGVVLQQ RWLLERTGSL    50
DKSFTRITWR PRADLARSLS VIENELSAGF SVYSNSSDVP ERFITNPVYN 100
SFHSEKFDIE QYLPPEVDLN LSWNPEDFTY DISVEPTQIQ IVEYRLLKQG 150
EEFTIARVKD EKLEVGVFFV DASDESDVDI GGIRCNWRMD DGKMERCQKT 200
SLLYKQGHIA YNHSTTTTSL YLNEPIGLHP KIMIDLTDFE ERPKCMYLMH 250
LQLPLELFID KFQSSPLLLF GEDDLELPEY SLRDKAWGSE SIFELKAGTM 300
NEVTLHTRYI EPSNNKGDKL EVSFDPEVIL ACDTGDNKVS RNPFYKKGLG 350
YESLFTDDTT FRHLNSTTLL VPIPRPDTKD YSKIKNGTLL CLLISIIYIF 400
SKVFGNNKKK RSVKRE 416
Length:416
Mass (Da):47,923
Last modified:May 1, 1992 - v1
Checksum:i65F7B920F0C2BBF0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42392.1.
AY692756 Genomic DNA. Translation: AAT92775.1.
BK006937 Genomic DNA. Translation: DAA07433.1.
PIRiS19382.
RefSeqiNP_009878.1. NM_001178697.1.

Genome annotation databases

EnsemblFungiiYCL052C; YCL052C; YCL052C.
GeneIDi850305.
KEGGisce:YCL052C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42392.1 .
AY692756 Genomic DNA. Translation: AAT92775.1 .
BK006937 Genomic DNA. Translation: DAA07433.1 .
PIRi S19382.
RefSeqi NP_009878.1. NM_001178697.1.

3D structure databases

ProteinModelPortali P25580.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 30933. 31 interactions.
DIPi DIP-8257N.
IntActi P25580. 5 interactions.
MINTi MINT-1355074.
STRINGi 4932.YCL052C.

Proteomic databases

MaxQBi P25580.
PaxDbi P25580.
PeptideAtlasi P25580.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCL052C ; YCL052C ; YCL052C .
GeneIDi 850305.
KEGGi sce:YCL052C.

Organism-specific databases

CYGDi YCL052c.
SGDi S000000557. PBN1.

Phylogenomic databases

eggNOGi NOG86258.
HOGENOMi HOG000115465.
KOi K07541.
OMAi SGLRCTW.
OrthoDBi EOG783N68.

Enzyme and pathway databases

UniPathwayi UPA00196 .
BioCyci YEAST:G3O-29306-MONOMER.
BRENDAi 3.4.21.48. 984.

Miscellaneous databases

NextBioi 965691.

Gene expression databases

Genevestigatori P25580.

Family and domain databases

InterProi IPR013233. PIG-X/PBN1.
[Graphical view ]
Pfami PF08320. PIG-X. 1 hit.
[Graphical view ]
SMARTi SM00780. PIG-X. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Multiple copies of PBS2, MHP1 or LRE1 produce glucanase resistance and other cell wall effects in Saccharomyces cerevisiae."
    Lai M.H., Silverman S.J., Gaughran J.P., Kirsch D.R.
    Yeast 13:199-213(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "The PBN1 gene of Saccharomyces cerevisiae: an essential gene that is required for the post-translational processing of the protease B precursor."
    Naik R.R., Jones E.W.
    Genetics 149:1277-1292(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, FUNCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Mammalian PIG-X and yeast Pbn1p are the essential components of glycosylphosphatidylinositol-mannosyltransferase I."
    Ashida H., Hong Y., Murakami Y., Shishioh N., Sugimoto N., Kim Y.U., Maeda Y., Kinoshita T.
    Mol. Biol. Cell 16:1439-1448(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Pbn1p: an essential endoplasmic reticulum membrane protein required for protein processing in the endoplasmic reticulum of budding yeast."
    Subramanian S., Woolford C.A., Drill E., Lu M., Jones E.W.
    Proc. Natl. Acad. Sci. U.S.A. 103:939-944(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPBN1_YEAST
AccessioniPrimary (citable) accession number: P25580
Secondary accession number(s): D6VQW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 14, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4930 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

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