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Protein

Protein PBN1

Gene

PBN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for proper folding and/or the stability of a subset of proteins in the endoplasmic reticulum. Aids the autocatalytic processing of PRB1. Component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase GPI14.3 Publications

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • GPI anchor biosynthetic process Source: SGD
  • mannosylation Source: GOC
  • protein processing Source: SGD
Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-29306-MONOMER.
BRENDAi3.4.21.48. 984.
UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PBN1
Alternative name(s):
Protease B non-derepressible protein 1
Gene namesi
Name:PBN1
Ordered Locus Names:YCL052C
ORF Names:YCL52C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL052C.
SGDiS000000557. PBN1.

Subcellular locationi

  • Endoplasmic reticulum membrane 1 Publication; Single-pass type III membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 385LumenalSequence analysisAdd BLAST385
Transmembranei386 – 405Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST20
Topological domaini406 – 416CytoplasmicSequence analysisAdd BLAST11

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • glycosylphosphatidylinositol-mannosyltransferase I complex Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000582431 – 416Protein PBN1Add BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...)Sequence analysis1
Glycosylationi85N-linked (GlcNAc...)Sequence analysis1
Glycosylationi120N-linked (GlcNAc...)Sequence analysis1
Glycosylationi212N-linked (GlcNAc...)Sequence analysis1
Glycosylationi365N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP25580.
PRIDEiP25580.

Interactioni

Protein-protein interaction databases

BioGridi30933. 32 interactors.
DIPiDIP-8257N.
IntActiP25580. 5 interactors.
MINTiMINT-1355074.

Structurei

3D structure databases

ProteinModelPortaliP25580.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGX family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000115465.
InParanoidiP25580.
KOiK07541.
OMAiSGLRCTW.
OrthoDBiEOG092C4TF1.

Family and domain databases

InterProiIPR013233. PIG-X/PBN1.
[Graphical view]
PfamiPF08320. PIG-X. 1 hit.
[Graphical view]
SMARTiSM00780. PIG-X. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25580-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTRHRVTVL YNAPEDIGNH MRQNDTHLTV RGGSGVVLQQ RWLLERTGSL
60 70 80 90 100
DKSFTRITWR PRADLARSLS VIENELSAGF SVYSNSSDVP ERFITNPVYN
110 120 130 140 150
SFHSEKFDIE QYLPPEVDLN LSWNPEDFTY DISVEPTQIQ IVEYRLLKQG
160 170 180 190 200
EEFTIARVKD EKLEVGVFFV DASDESDVDI GGIRCNWRMD DGKMERCQKT
210 220 230 240 250
SLLYKQGHIA YNHSTTTTSL YLNEPIGLHP KIMIDLTDFE ERPKCMYLMH
260 270 280 290 300
LQLPLELFID KFQSSPLLLF GEDDLELPEY SLRDKAWGSE SIFELKAGTM
310 320 330 340 350
NEVTLHTRYI EPSNNKGDKL EVSFDPEVIL ACDTGDNKVS RNPFYKKGLG
360 370 380 390 400
YESLFTDDTT FRHLNSTTLL VPIPRPDTKD YSKIKNGTLL CLLISIIYIF
410
SKVFGNNKKK RSVKRE
Length:416
Mass (Da):47,923
Last modified:May 1, 1992 - v1
Checksum:i65F7B920F0C2BBF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42392.1.
AY692756 Genomic DNA. Translation: AAT92775.1.
BK006937 Genomic DNA. Translation: DAA07433.1.
PIRiS19382.
RefSeqiNP_009878.1. NM_001178697.1.

Genome annotation databases

EnsemblFungiiCAA42392; CAA42392; CAA42392.
YCL052C; YCL052C; YCL052C.
GeneIDi850305.
KEGGisce:YCL052C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42392.1.
AY692756 Genomic DNA. Translation: AAT92775.1.
BK006937 Genomic DNA. Translation: DAA07433.1.
PIRiS19382.
RefSeqiNP_009878.1. NM_001178697.1.

3D structure databases

ProteinModelPortaliP25580.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30933. 32 interactors.
DIPiDIP-8257N.
IntActiP25580. 5 interactors.
MINTiMINT-1355074.

Proteomic databases

MaxQBiP25580.
PRIDEiP25580.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42392; CAA42392; CAA42392.
YCL052C; YCL052C; YCL052C.
GeneIDi850305.
KEGGisce:YCL052C.

Organism-specific databases

EuPathDBiFungiDB:YCL052C.
SGDiS000000557. PBN1.

Phylogenomic databases

HOGENOMiHOG000115465.
InParanoidiP25580.
KOiK07541.
OMAiSGLRCTW.
OrthoDBiEOG092C4TF1.

Enzyme and pathway databases

UniPathwayiUPA00196.
BioCyciYEAST:G3O-29306-MONOMER.
BRENDAi3.4.21.48. 984.

Miscellaneous databases

PROiP25580.

Family and domain databases

InterProiIPR013233. PIG-X/PBN1.
[Graphical view]
PfamiPF08320. PIG-X. 1 hit.
[Graphical view]
SMARTiSM00780. PIG-X. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPBN1_YEAST
AccessioniPrimary (citable) accession number: P25580
Secondary accession number(s): D6VQW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4930 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.