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Protein

Nicotinamide mononucleotide adenylyltransferase

Gene

POF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Involved in the salvage pathway for NAD+ biosynthesis via NMN (PubMed:24759102). Involved in the filamentation pathway. Suppresses the filamentation defect of a KSS1 deletion (PubMed:21460040).2 Publications

Catalytic activityi

ATP + nicotinamide ribonucleotide = diphosphate + NAD+.1 Publication

Kineticsi

  1. KM=2.26 mM for nicotinamide mononucleotide1 Publication
  1. Vmax=119 nmol/h/mg enzyme1 Publication

pH dependencei

Optimum pH is 10.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi34 – 363ATPBy similarity
Nucleotide bindingi138 – 1403ATPBy similarity
Nucleotide bindingi224 – 2274ATPBy similarity

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • nicotinamide-nucleotide adenylyltransferase activity Source: SGD

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • invasive growth in response to glucose limitation Source: SGD
  • NAD biosynthesis via nicotinamide riboside salvage pathway Source: SGD
  • pseudohyphal growth Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29302-MONOMER.
UniPathwayiUPA00253; UER00600.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide mononucleotide adenylyltransferase1 Publication (EC:2.7.7.11 Publication)
Short name:
NMN adenylyltransferase
Short name:
NMNAT
Alternative name(s):
NMN-specific adenylyltransferase1 Publication
Promoter of filamentation protein 11 Publication
Gene namesi
Name:POF11 Publication
Ordered Locus Names:YCL047CImported
ORF Names:YCL47C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome III

Organism-specific databases

CYGDiYCL047c.
EuPathDBiFungiDB:YCL047C.
SGDiS000000552. POF1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Lowers cellular NAD+ levels and decreases the efficiency of nicotinamide riboside (NR) utilization, resistance to oxidative stress, and NR-induced life span extension (PubMed:24759102). Highly sensitive to heat shock and higher sensitivity to ER stress agents than wild-type cells (PubMed:22204397).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258Nicotinamide mononucleotide adenylyltransferasePRO_0000202549Add
BLAST

Proteomic databases

MaxQBiP25576.
PaxDbiP25576.
PeptideAtlasiP25576.

Expressioni

Inductioni

Expression is slightly induced in late log phase.1 Publication

Gene expression databases

GenevestigatoriP25576.

Interactioni

Subunit structurei

Interacts with KSS1 (PubMed:21460040). Interacts with UBC7 (PubMed:22204397).2 Publications

Protein-protein interaction databases

BioGridi30938. 6 interactions.
IntActiP25576. 3 interactions.
MINTiMINT-2492497.
STRINGi4932.YCL047C.

Structurei

3D structure databases

ProteinModelPortaliP25576.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG77130.
InParanoidiP25576.
OMAiTNHAKFV.
OrthoDBiEOG7BGHZG.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25576-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKTFEQFRK SNLLFQVLKG PQHLECQKLF VLDSSFNPPH LAHFQLLSQT
60 70 80 90 100
IKNFKLKDTR SHVLLLLAVN NADKLPKPAS FPTRLEMMCL FADYLQEKLP
110 120 130 140 150
QSVVSVGLTV FSKFIDKDKI LHEQFVKGCS ADIGYLVGFD TIARIFDEKY
160 170 180 190 200
YHPLKISDVM ESFMSGSQLY CLARGDCHLS AESQLRYASD ILEGKFEPVI
210 220 230 240 250
PREWGARIHV MQNDYPALRN VSSSEIRNKL KNGQVESLKD ELPLCIYDYL

INNKTIFD
Length:258
Mass (Da):29,673
Last modified:May 1, 1992 - v1
Checksum:i1A55281FC19854C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42369.1.
BK006937 Genomic DNA. Translation: DAA07439.1.
PIRiS19376.
RefSeqiNP_009883.1. NM_001178692.1.

Genome annotation databases

EnsemblFungiiYCL047C; YCL047C; YCL047C.
GeneIDi850310.
KEGGisce:YCL047C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42369.1.
BK006937 Genomic DNA. Translation: DAA07439.1.
PIRiS19376.
RefSeqiNP_009883.1. NM_001178692.1.

3D structure databases

ProteinModelPortaliP25576.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30938. 6 interactions.
IntActiP25576. 3 interactions.
MINTiMINT-2492497.
STRINGi4932.YCL047C.

Proteomic databases

MaxQBiP25576.
PaxDbiP25576.
PeptideAtlasiP25576.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYCL047C; YCL047C; YCL047C.
GeneIDi850310.
KEGGisce:YCL047C.

Organism-specific databases

CYGDiYCL047c.
EuPathDBiFungiDB:YCL047C.
SGDiS000000552. POF1.

Phylogenomic databases

eggNOGiNOG77130.
InParanoidiP25576.
OMAiTNHAKFV.
OrthoDBiEOG7BGHZG.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00600.
BioCyciYEAST:G3O-29302-MONOMER.

Miscellaneous databases

NextBioi965706.
PROiP25576.

Gene expression databases

GenevestigatoriP25576.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  4. "The promoter of filamentation (POF1) protein from Saccharomyces cerevisiae is an ATPase involved in the protein quality control process."
    Costa I.M., Nasser T.H., Demasi M., Nascimento R.M., Netto L.E., Miyamoto S., Prado F.M., Monteiro G.
    BMC Microbiol. 11:268-268(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBC7, DISRUPTION PHENOTYPE.
  5. "Diverse protein kinase interactions identified by protein microarrays reveal novel connections between cellular processes."
    Fasolo J., Sboner A., Sun M.G., Yu H., Chen R., Sharon D., Kim P.M., Gerstein M., Snyder M.
    Genes Dev. 25:767-778(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KSS1.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "YCL047C/POF1 is a novel nicotinamide mononucleotide adenylyltransferase (NMNAT) in Saccharomyces cerevisiae."
    Kato M., Lin S.J.
    J. Biol. Chem. 289:15577-15587(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, INDUCTION.
    Strain: ATCC 201389 / BY4742.

Entry informationi

Entry nameiPOF1_YEAST
AccessioniPrimary (citable) accession number: P25576
Secondary accession number(s): D6VQX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 29, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 830 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.