P25569 (GID7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-induced degradation protein 7

Gene names

Name:	GID7
Synonyms:	MOH2
Ordered Locus Names:	YCL039W
ORF Names:	YCL311, YCL39W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	745 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a role in the negative regulation of gluconeogenesis. Required for proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase). Ref.4
Subcellular location	Nucleus. Cytoplasm Ref.5.
Miscellaneous	Present with 1200 molecules/cell in log phase SD medium.
Sequence similarities	Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of gluconeogenesis Inferred from mutant phenotype Ref.4. Source: SGD proteasomal ubiquitin-dependent protein catabolic process Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	GID complex Inferred from direct assay PubMed 18508925. Source: SGD cytoplasm Inferred from direct assay Ref.5. Source: SGD nucleus Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GID8	P40208	6	EBI-21727,EBI-27276

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 745	745	Glucose-induced degradation protein 7		PRO_0000051006
Regions
	Repeat	167 – 209	43	WD 1
	Repeat	322 – 365	44	WD 2
	Repeat	369 – 408	40	WD 3
	Repeat	540 – 579	40	WD 4
	Repeat	613 – 652	40	WD 5
	Repeat	657 – 696	40	WD 6
	Repeat	710 – 745	36	WD 7
Amino acid modifications
	Modified residue	291	1	Phosphoserine Ref.8
	Modified residue	293	1	Phosphoserine Ref.8
	Modified residue	294	1	Phosphoserine Ref.7
	Modified residue	444	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P25569 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: 558D538AD27E3B71	FASTA	745	84,517
	10 20 30 40 50 60 MSHTNKIAYV LNNDTEETAS PSSVGCFDKK QLTKLLIHTL KELGYDSAAN QLLLESGGYQ 70 80 90 100 110 120 NESNHIQTFF KLIKTGQFHL INWQIVCSLP LAHSSPLRSE WLQRLLIPTP TPATTSLFDH 130 140 150 160 170 180 MLLQLQYLQQ LMSSVNSSTC SDAEIATLRN YVEIMILVNR QIFLEFFHPV TNSASHKGPH 190 200 210 220 230 240 TALPVLYLRK ILKNFIEIWD SLLVSNDQFL NEENIFNPET TLRELSTYLT NPKLTAQLNL 250 260 270 280 290 300 ERDHLIDAIS KYIDPNELVP KGRLLHLLKQ AIKYQQSQDI FNIIDPDDDA SFSSPPHRIN 310 320 330 340 350 360 LLQDNFSHDL TVTFQEWKTI QDTTDEIWFL TFSPNGKYLA SATSESSRGY FITVYDVEQD 370 380 390 400 410 420 FKIYKTCVSL SQSVLYLMFS PDSRYLVACP FSEDVTIYDM NATSLPDASA TDSFLLYPST 430 440 450 460 470 480 RLSPMDSFKL DTTTYPDDTE SSASSSSRPA NANSNQSRVW CCDAFHTAER AGWMVVGSPD 490 500 510 520 530 540 REAIVHSLTT KESLFSLKGR TCIALGHDEN ISGRKSIDPA KVLYKPTSSN GNWQYVEDDE 550 560 570 580 590 600 TFPRVHDVKI SYDDKYVLLM THQGVIDVYD FSGFPSKEEL SKQTVDPKNF LIPRIARLDV 610 620 630 640 650 660 GKNMTCISLP LNTTHQGFHR QQISESQHLV LVSLQDNELQ MWDYKENILI QKYFGQKQQH 670 680 690 700 710 720 FIIRSCFAYG NKLVMSGSED GKIYIWDRIR GNLVSVLSGH STVMSNSTKP MGKNCNVVAS 730 740 NPADKEMFAS GGDDGKIKIW KISRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of a 9,543 bp segment on the left arm of chromosome III reveals five open reading frames including glucokinase and the protein disulfide isomerase." Scherens B., Messenguy F., Gigot D., Dubois E. Yeast 8:577-586(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways." Regelmann J., Schuele T., Josupeit F.S., Horak J., Rose M., Entian K.-D., Thumm M., Wolf D.H. Mol. Biol. Cell 14:1652-1663(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294 AND SER-444, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-291 AND SER-293, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59720 Genomic DNA. Translation: CAA42377.1. BK006937 Genomic DNA. Translation: DAA07445.1.
PIR	S74281.
RefSeq	NP_009891.1. NM_001178684.1.
3D structure databases
ProteinModelPortal	P25569.
SMR	P25569. Positions 310-401, 622-741.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4496N.
IntAct	P25569. 12 interactions.
MINT	MINT-572840.
STRING	4932.YCL039W.
Proteomic databases
PaxDb	P25569.
PeptideAtlas	P25569.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCL039W; YCL039W; YCL039W.
GeneID	850318.
KEGG	sce:YCL039W.
Organism-specific databases
CYGD	YCL039w.
SGD	S000000544. GID7.
Phylogenomic databases
eggNOG	NOG281318.
GeneTree	ENSGT00530000063585.
HOGENOM	HOG000057092.
OMA	NPNDLVP.
OrthoDB	EOG43R6WJ.
Gene expression databases
Genevestigator	P25569.
GermOnline	YCL039W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 3 hits.
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 2 hits. [Graphical view]
SMART	SM00320. WD40. 5 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 2 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	965724.

Entry information

Entry name

GID7_YEAST

Accession

Primary (citable) accession number: P25569
Secondary accession number(s): D6VQX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	September 5, 2006
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program