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Protein

Autophagy-related protein 22

Gene

ATG22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Vacuolar effluxer which mediate the efflux of leucine and other amino acids resulting from autophagic degradation. The release of autophagic amino acids allows the maintenance of protein synthesis and viability during nitrogen starvation.3 Publications

GO - Biological processi

  • amino acid transmembrane export from vacuole Source: SGD
  • autophagy Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Autophagy, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29297-MONOMER.

Protein family/group databases

TCDBi2.A.1.24.1. the major facilitator superfamily (mfs).
9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 22
Gene namesi
Name:ATG22
Synonyms:AUT4
Ordered Locus Names:YCL038C
ORF Names:YCL38C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome III

Organism-specific databases

CYGDiYCL038c.
EuPathDBiFungiDB:YCL038C.
SGDiS000000543. ATG22.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9898CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei99 – 11921HelicalSequence AnalysisAdd
BLAST
Topological domaini120 – 13011VacuolarSequence AnalysisAdd
BLAST
Transmembranei131 – 15121HelicalSequence AnalysisAdd
BLAST
Topological domaini152 – 1532CytoplasmicSequence Analysis
Transmembranei154 – 17421HelicalSequence AnalysisAdd
BLAST
Topological domaini175 – 21036VacuolarSequence AnalysisAdd
BLAST
Transmembranei211 – 23121HelicalSequence AnalysisAdd
BLAST
Topological domaini232 – 24110CytoplasmicSequence Analysis
Transmembranei242 – 26221HelicalSequence AnalysisAdd
BLAST
Topological domaini263 – 31856VacuolarSequence AnalysisAdd
BLAST
Transmembranei319 – 33921HelicalSequence AnalysisAdd
BLAST
Topological domaini340 – 35213CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei353 – 37321HelicalSequence AnalysisAdd
BLAST
Topological domaini374 – 38815VacuolarSequence AnalysisAdd
BLAST
Transmembranei389 – 40921HelicalSequence AnalysisAdd
BLAST
Topological domaini410 – 4178CytoplasmicSequence Analysis
Transmembranei418 – 43821HelicalSequence AnalysisAdd
BLAST
Topological domaini439 – 48547VacuolarSequence AnalysisAdd
BLAST
Transmembranei486 – 50621HelicalSequence AnalysisAdd
BLAST
Topological domaini507 – 52822CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of fungal-type vacuolar membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 528528Autophagy-related protein 22PRO_0000207629Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei278 – 2781Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25568.
PaxDbiP25568.
PeptideAtlasiP25568.

Interactioni

Protein-protein interaction databases

BioGridi30945. 17 interactions.
DIPiDIP-4976N.
IntActiP25568. 3 interactions.
MINTiMINT-478200.

Structurei

3D structure databases

ProteinModelPortaliP25568.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG22 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2270.
HOGENOMiHOG000034115.
InParanoidiP25568.
KOiK06902.
OMAiIRPAFWF.
OrthoDBiEOG71GB3P.

Family and domain databases

InterProiIPR024671. Atg22-like.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF11700. ATG22. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.

Sequencei

Sequence statusi: Complete.

P25568-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYGTINDMN ESVTNYRIKK AQNNIKGWYA YSFSSEPFVV SAVSTYIPLL
60 70 80 90 100
LQQFASINGV KVHDHSIPCL SETGSDSDKC VLGLFNNRIF VDTSSFALYV
110 120 130 140 150
FSLSVLFQTI IVISVSGIVD LWGSVKFKGR ILVWFGIVGA LSTVAISKLN
160 170 180 190 200
DTQIYSLAGL YIVANGCFGV INVVGNSLLP IFVKDSLKCQ SQGAYEPDKV
210 220 230 240 250
DSLTTVISGR GASLGYSSAL IVQIVSMFLV ASKKGSKQDV QVAVLFVGIW
260 270 280 290 300
WFVWQLPMIW LIDDVTIPIR VDDSTLASAR SPYPGEQDAL GQLNWKNYLS
310 320 330 340 350
YGWVSLFESF KHARLLKDVM IFLIAWFIIS DSITTINSTA VLFSKAELHM
360 370 380 390 400
STLNLIMISV LTVVNAMLGA FMIPQFLATK FRWTSSQTLM YIIIWASFIP
410 420 430 440 450
FYGILGFFFN AFGLKHKFEM FLLAIWYGLS LGGLSAVSRS VFSLIVPPGK
460 470 480 490 500
ESTFFSMFSI TDKGSSILGP FLVGLLTDKT HNIRYSFYFF FLLLMLSLPV
510 520
LNCLDVKRGR REAEELSQVL PESERRLD
Length:528
Mass (Da):58,844
Last modified:May 1, 1992 - v1
Checksum:iFC26CAFD581A66DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42378.1.
BK006937 Genomic DNA. Translation: DAA07446.1.
PIRiS19366.
RefSeqiNP_009892.1. NM_001178683.1.

Genome annotation databases

EnsemblFungiiYCL038C; YCL038C; YCL038C.
GeneIDi850319.
KEGGisce:YCL038C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42378.1.
BK006937 Genomic DNA. Translation: DAA07446.1.
PIRiS19366.
RefSeqiNP_009892.1. NM_001178683.1.

3D structure databases

ProteinModelPortaliP25568.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30945. 17 interactions.
DIPiDIP-4976N.
IntActiP25568. 3 interactions.
MINTiMINT-478200.

Protein family/group databases

TCDBi2.A.1.24.1. the major facilitator superfamily (mfs).
9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

Proteomic databases

MaxQBiP25568.
PaxDbiP25568.
PeptideAtlasiP25568.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYCL038C; YCL038C; YCL038C.
GeneIDi850319.
KEGGisce:YCL038C.

Organism-specific databases

CYGDiYCL038c.
EuPathDBiFungiDB:YCL038C.
SGDiS000000543. ATG22.

Phylogenomic databases

eggNOGiCOG2270.
HOGENOMiHOG000034115.
InParanoidiP25568.
KOiK06902.
OMAiIRPAFWF.
OrthoDBiEOG71GB3P.

Enzyme and pathway databases

BioCyciYEAST:G3O-29297-MONOMER.

Miscellaneous databases

NextBioi965727.
PROiP25568.

Family and domain databases

InterProiIPR024671. Atg22-like.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF11700. ATG22. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway."
    Harding T.M., Hefner-Gravink A., Thumm M., Klionsky D.J.
    J. Biol. Chem. 271:17621-17624(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "The breakdown of autophagic vesicles inside the vacuole depends on Aut4p."
    Suriapranata I., Epple U.D., Bernreuther D., Bredschneider M., Sovarasteanu K., Thumm M.
    J. Cell Sci. 113:4025-4033(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: NOMENCLATURE.
  6. "Atg22 recycles amino acids to link the degradative and recycling functions of autophagy."
    Yang Z., Huang J., Geng J., Nair U., Klionsky D.J.
    Mol. Biol. Cell 17:5094-5104(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiATG22_YEAST
AccessioniPrimary (citable) accession number: P25568
Secondary accession number(s): D6VQX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: July 22, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.