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P25568

- ATG22_YEAST

UniProt

P25568 - ATG22_YEAST

Protein

Autophagy-related protein 22

Gene

ATG22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Vacuolar effluxer which mediate the efflux of leucine and other amino acids resulting from autophagic degradation. The release of autophagic amino acids allows the maintenance of protein synthesis and viability during nitrogen starvation.3 Publications

    GO - Biological processi

    1. amino acid transmembrane export from vacuole Source: SGD
    2. autophagy Source: UniProtKB-KW

    Keywords - Biological processi

    Amino-acid transport, Autophagy, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29297-MONOMER.

    Protein family/group databases

    TCDBi2.A.1.24.1. the major facilitator superfamily (mfs).
    9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Autophagy-related protein 22
    Gene namesi
    Name:ATG22
    Synonyms:AUT4
    Ordered Locus Names:YCL038C
    ORF Names:YCL38C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCL038c.
    SGDiS000000543. ATG22.

    Subcellular locationi

    Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Vacuole and punctate structures.

    GO - Cellular componenti

    1. integral component of fungal-type vacuolar membrane Source: SGD

    Keywords - Cellular componenti

    Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 528528Autophagy-related protein 22PRO_0000207629Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei278 – 2781Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP25568.
    PaxDbiP25568.
    PeptideAtlasiP25568.

    Expressioni

    Gene expression databases

    GenevestigatoriP25568.

    Interactioni

    Protein-protein interaction databases

    BioGridi30945. 18 interactions.
    DIPiDIP-4976N.
    IntActiP25568. 3 interactions.
    MINTiMINT-478200.
    STRINGi4932.YCL038C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25568.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9898CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini120 – 13011VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini152 – 1532CytoplasmicSequence Analysis
    Topological domaini175 – 21036VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini232 – 24110CytoplasmicSequence Analysis
    Topological domaini263 – 31856VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini340 – 35213CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini374 – 38815VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini410 – 4178CytoplasmicSequence Analysis
    Topological domaini439 – 48547VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini507 – 52822CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei99 – 11921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei131 – 15121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei154 – 17421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei211 – 23121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei242 – 26221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei319 – 33921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei353 – 37321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei389 – 40921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei418 – 43821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei486 – 50621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATG22 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2270.
    HOGENOMiHOG000034115.
    KOiK06902.
    OMAiIRPAFWF.
    OrthoDBiEOG71GB3P.

    Family and domain databases

    InterProiIPR024671. Atg22-like.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view]
    PfamiPF11700. ATG22. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P25568-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYGTINDMN ESVTNYRIKK AQNNIKGWYA YSFSSEPFVV SAVSTYIPLL    50
    LQQFASINGV KVHDHSIPCL SETGSDSDKC VLGLFNNRIF VDTSSFALYV 100
    FSLSVLFQTI IVISVSGIVD LWGSVKFKGR ILVWFGIVGA LSTVAISKLN 150
    DTQIYSLAGL YIVANGCFGV INVVGNSLLP IFVKDSLKCQ SQGAYEPDKV 200
    DSLTTVISGR GASLGYSSAL IVQIVSMFLV ASKKGSKQDV QVAVLFVGIW 250
    WFVWQLPMIW LIDDVTIPIR VDDSTLASAR SPYPGEQDAL GQLNWKNYLS 300
    YGWVSLFESF KHARLLKDVM IFLIAWFIIS DSITTINSTA VLFSKAELHM 350
    STLNLIMISV LTVVNAMLGA FMIPQFLATK FRWTSSQTLM YIIIWASFIP 400
    FYGILGFFFN AFGLKHKFEM FLLAIWYGLS LGGLSAVSRS VFSLIVPPGK 450
    ESTFFSMFSI TDKGSSILGP FLVGLLTDKT HNIRYSFYFF FLLLMLSLPV 500
    LNCLDVKRGR REAEELSQVL PESERRLD 528
    Length:528
    Mass (Da):58,844
    Last modified:May 1, 1992 - v1
    Checksum:iFC26CAFD581A66DB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42378.1.
    BK006937 Genomic DNA. Translation: DAA07446.1.
    PIRiS19366.
    RefSeqiNP_009892.1. NM_001178683.1.

    Genome annotation databases

    EnsemblFungiiYCL038C; YCL038C; YCL038C.
    GeneIDi850319.
    KEGGisce:YCL038C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42378.1 .
    BK006937 Genomic DNA. Translation: DAA07446.1 .
    PIRi S19366.
    RefSeqi NP_009892.1. NM_001178683.1.

    3D structure databases

    ProteinModelPortali P25568.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30945. 18 interactions.
    DIPi DIP-4976N.
    IntActi P25568. 3 interactions.
    MINTi MINT-478200.
    STRINGi 4932.YCL038C.

    Protein family/group databases

    TCDBi 2.A.1.24.1. the major facilitator superfamily (mfs).
    9.A.15.1.1. the autophagy-related phagophore-formation transporter (apt) family.

    Proteomic databases

    MaxQBi P25568.
    PaxDbi P25568.
    PeptideAtlasi P25568.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCL038C ; YCL038C ; YCL038C .
    GeneIDi 850319.
    KEGGi sce:YCL038C.

    Organism-specific databases

    CYGDi YCL038c.
    SGDi S000000543. ATG22.

    Phylogenomic databases

    eggNOGi COG2270.
    HOGENOMi HOG000034115.
    KOi K06902.
    OMAi IRPAFWF.
    OrthoDBi EOG71GB3P.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29297-MONOMER.

    Miscellaneous databases

    NextBioi 965727.

    Gene expression databases

    Genevestigatori P25568.

    Family and domain databases

    InterProi IPR024671. Atg22-like.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view ]
    Pfami PF11700. ATG22. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway."
      Harding T.M., Hefner-Gravink A., Thumm M., Klionsky D.J.
      J. Biol. Chem. 271:17621-17624(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "The breakdown of autophagic vesicles inside the vacuole depends on Aut4p."
      Suriapranata I., Epple U.D., Bernreuther D., Bredschneider M., Sovarasteanu K., Thumm M.
      J. Cell Sci. 113:4025-4033(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: NOMENCLATURE.
    6. "Atg22 recycles amino acids to link the degradative and recycling functions of autophagy."
      Yang Z., Huang J., Geng J., Nair U., Klionsky D.J.
      Mol. Biol. Cell 17:5094-5104(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiATG22_YEAST
    AccessioniPrimary (citable) accession number: P25568
    Secondary accession number(s): D6VQX7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3