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Protein

Peptide methionine sulfoxide reductase 2

Gene

MXR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Methionine-R-sulfoxide reductase which catalyzes the reduction of methionine sulfoxide (MetSO) to methionine in proteins. Plays a protective role against oxidative stress by restoring activity to proteins that have been inactivated by methionine oxidation. Protects iron-sulfur clusters from oxidative inactivation along with MXR1. Involved in the regulation of lifespan.2 Publications

Miscellaneous

Present with 799 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi79ZincPROSITE-ProRule annotation1
Metal bindingi82ZincPROSITE-ProRule annotation1
Metal bindingi128ZincPROSITE-ProRule annotation1
Metal bindingi131ZincPROSITE-ProRule annotation1
Active sitei157NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • peptide-methionine (R)-S-oxide reductase activity Source: SGD

GO - Biological processi

  • cellular response to oxidative stress Source: SGD
  • protein repair Source: InterPro

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29293-MONOMER
ReactomeiR-SCE-5676934 Protein repair

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide methionine sulfoxide reductase 2 (EC:1.8.4.12)
Gene namesi
Name:MXR2
Synonyms:MSRB
Ordered Locus Names:YCL033C
ORF Names:YCL33C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL033C
SGDiS000000538 MXR2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001403261 – 168Peptide methionine sulfoxide reductase 2Add BLAST168

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi97 ↔ 157Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP25566
PaxDbiP25566
PRIDEiP25566

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi30950, 39 interactors
DIPiDIP-4861N
IntActiP25566, 7 interactors
STRINGi4932.YCL033C

Structurei

3D structure databases

ProteinModelPortaliP25566
SMRiP25566
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 168MsrBPROSITE-ProRule annotationAdd BLAST129

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

GeneTreeiENSGT00510000046802
InParanoidiP25566
KOiK07305
OMAiYKASHKF
OrthoDBiEOG092C584K

Family and domain databases

InterProiView protein in InterPro
IPR028427 Met_Sox_Rdtase
IPR002579 Met_Sox_Rdtase_MsrB
IPR011057 Mss4-like_sf
PANTHERiPTHR10173 PTHR10173, 1 hit
PfamiView protein in Pfam
PF01641 SelR, 1 hit
SUPFAMiSSF51316 SSF51316, 1 hit
TIGRFAMsiTIGR00357 TIGR00357, 1 hit
PROSITEiView protein in PROSITE
PS51790 MSRB, 1 hit

Sequencei

Sequence statusi: Complete.

P25566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKWSRLYVI TVRRTFPGRR NIVLTQYWNK SKKMSDESND VKWNDALTPL
60 70 80 90 100
QLMVLRDKAT ERPNTGAYLH TNESGVYHCA NCDRPLYSSK AKFDARCGWP
110 120 130 140 150
AFYEEVSPGA ITYHRDNSLM PARVEICCAR CGGHLGHVFE GEGWKQLLNL
160
PKDTRHCVNS ASLNLKKD
Length:168
Mass (Da):19,279
Last modified:May 1, 1992 - v1
Checksum:iC79BE75DC35D82F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA Translation: CAA42383.1
BK006937 Genomic DNA Translation: DAA07451.1
PIRiS19361
RefSeqiNP_009897.1, NM_001178678.1

Genome annotation databases

EnsemblFungiiCAA42383; CAA42383; CAA42383
YCL033C; YCL033C; YCL033C
GeneIDi850324
KEGGisce:YCL033C

Similar proteinsi

Entry informationi

Entry nameiMXR2_YEAST
AccessioniPrimary (citable) accession number: P25566
Secondary accession number(s): D6VQY2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: March 28, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health