P25560 (RER1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein RER1

Alternative name(s):
Retention of ER proteins 1

Gene names

Name:	RER1
Ordered Locus Names:	YCL001W
ORF Names:	YCL1W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment. Required for correct localization of SEC12, SEC71 and SEC63 in the endoplasmic reticulum. Ref.5
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein.
Miscellaneous	Present with 2840 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the RER1 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from physical interaction PubMed 11157978. Source: SGD protein retention in ER lumen Inferred from mutant phenotype PubMed 8707815. Source: SGD retrograde vesicle-mediated transport, Golgi to ER Inferred from mutant phenotype PubMed 11238450. Source: SGD
Cellular_component	COPI-coated vesicle Inferred from direct assay PubMed 11238450. Source: SGD ER to Golgi transport vesicle Inferred from direct assay PubMed 11157978. Source: SGD Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Protein RER1		PRO_0000207593
Regions
	Topological domain	1 – 61	61	Cytoplasmic Potential
	Transmembrane	62 – 82	21	Helical; Potential
	Topological domain	83 – 138	56	Lumenal Potential
	Transmembrane	139 – 161	23	Helical; Potential
	Topological domain	162 – 188	27	Cytoplasmic Potential
Experimental info
	Sequence conflict	109 – 125	17	RPFIR…WYNSI → LSIHQKTYQSSNSGITAF Ref.2
	Sequence conflict	136	1	L → V Ref.2
	Sequence conflict	141	1	D → S Ref.2
	Sequence conflict	145 – 188	44	FWPIL…SHSSN → IFAHSLFDCYFHYYCFF Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P25560 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: ACD0DAFA757C3622	FASTA	188	22,270
	10 20 30 40 50 60 MDYDSSDTMN GGSSNPLITK MNTMKLLYQH YLDKVTPHAK ERWAVLGGLL CLFMVRITMA 70 80 90 100 110 120 EGWYVICYGL GLFLLNQFLA FLTPKFDMSL QQDEENNELE AGEKSEEFRP FIRRLPEFKF 130 140 150 160 170 180 WYNSIRATVI SLLLSLFSIF DIPVFWPILL MYFILLFFLT MRRQIQHMIK YRYIPLDIGK KKYSHSSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Membrane protein retrieval from the Golgi apparatus to the endoplasmic reticulum (ER): characterization of the RER1 gene product as a component involved in ER localization of Sec12p." Sato K., Nishikawa S., Nakano A. Mol. Biol. Cell 6:1459-1477(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Identification of a gene required for membrane protein retention in the early secretory pathway." Nishikawa S., Nakano A. Proc. Natl. Acad. Sci. U.S.A. 90:8179-8183(1993) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Rer1p as common machinery for the endoplasmic reticulum localization of membrane proteins." Sato K., Sato M., Nakano A. Proc. Natl. Acad. Sci. U.S.A. 94:9693-9698(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D28552 Genomic DNA. Translation: BAA05906.1. X59720 Genomic DNA. Translation: CAA42336.1. BK006937 Genomic DNA. Translation: DAA07478.1.
PIR	S50158.
RefSeq	NP_009925.1. NM_001178651.1.
3D structure databases
ProteinModelPortal	P25560.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4478009.
STRING	4932.YCL001W.
Protein family/group databases
TCDB	9.B.82.1.1. endoplasmic reticulum retrieval Protein1 (Putative heavy metal Transporter) (Rer1) family.
Proteomic databases
PaxDb	P25560.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCL001W; YCL001W; YCL001W.
GeneID	850354.
KEGG	sce:YCL001W.
Organism-specific databases
CYGD	YCL001w.
SGD	S000000507. RER1.
Phylogenomic databases
eggNOG	COG5249.
GeneTree	ENSGT00510000047137.
HOGENOM	HOG000163668.
OMA	TRRSDEY.
OrthoDB	EOG4N8VF5.
Enzyme and pathway databases
BioCyc	YEAST:G3O-29275-MONOMER.
Gene expression databases
Genevestigator	P25560.
GermOnline	YCL001W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004932. Rer1. [Graphical view]
PANTHER	PTHR10743. PTHR10743. 1 hit.
Pfam	PF03248. Rer1. 1 hit. [Graphical view]
PIRSF	PIRSF016013. AtER_Rer1p. 1 hit.
ProtoNet	Search...
Other
NextBio	965821.

Entry information

Entry name

RER1_YEAST

Accession

Primary (citable) accession number: P25560
Secondary accession number(s): D6VR09

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program