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Protein

Bud site selection protein 3

Gene

BUD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Co-assembles with BUD4 at bud sites. BUD4 and BUD3 may cooperate to recognize a spatial landmark (the neck filaments) during mitosis and they subsequently become a landmark for establishing the axial budding pattern in G1.

GO - Molecular functioni

  • Rho guanyl-nucleotide exchange factor activity Source: SGD

GO - Biological processi

  • axial cellular bud site selection Source: SGD
  • cytogamy Source: SGD
  • positive regulation of GTPase activity Source: SGD
  • regulation of Rho protein signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Enzyme and pathway databases

BioCyciYEAST:G3O-29281-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bud site selection protein 3
Gene namesi
Name:BUD3
Ordered Locus Names:YCL014W
ORF Names:YCL012W, YCL013W, YCL12W, YCL13W, YCL14W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL014W.
SGDiS000000520. BUD3.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud neck contractile ring Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000650161 – 1636Bud site selection protein 3Add BLAST1636

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei766PhosphoserineCombined sources1
Cross-linki792Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki963Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei1045PhosphothreonineCombined sources1
Modified residuei1063PhosphoserineCombined sources1
Modified residuei1075PhosphoserineCombined sources1
Modified residuei1134PhosphoserineCombined sources1
Modified residuei1149PhosphoserineCombined sources1
Modified residuei1157PhosphothreonineCombined sources1
Modified residuei1160PhosphoserineCombined sources1
Modified residuei1228PhosphoserineCombined sources1
Modified residuei1254PhosphoserineCombined sources1
Modified residuei1257PhosphoserineCombined sources1
Modified residuei1390PhosphoserineCombined sources1
Modified residuei1412PhosphoserineCombined sources1
Modified residuei1429PhosphothreonineCombined sources1
Modified residuei1440PhosphothreonineCombined sources1
Modified residuei1443PhosphoserineCombined sources1
Modified residuei1501PhosphoserineCombined sources1
Modified residuei1549PhosphoserineCombined sources1
Modified residuei1589PhosphoserineCombined sources1
Modified residuei1614PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP25558.
PRIDEiP25558.

PTM databases

iPTMnetiP25558.

Interactioni

Subunit structurei

Interacts with AXL2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AXL2P389282EBI-3840,EBI-3397

Protein-protein interaction databases

BioGridi30969. 64 interactors.
DIPiDIP-4969N.
IntActiP25558. 7 interactors.
MINTiMINT-522762.

Structurei

3D structure databases

ProteinModelPortaliP25558.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BUD3 family.Curated

Phylogenomic databases

HOGENOMiHOG000000894.
InParanoidiP25558.
OMAiYTEMKIE.
OrthoDBiEOG092C2ME1.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
InterProiIPR021895. Bud3_N.
IPR000219. DH-domain.
[Graphical view]
PfamiPF12015. DUF3507. 1 hit.
[Graphical view]
SMARTiSM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.

Sequencei

Sequence statusi: Complete.

P25558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKDLSSLYS EKKDKENDET LFNIKLSKSV VETTPLNGHS LFDDDKSLSD
60 70 80 90 100
WTDNVFTQSV FYHGSDDLIW GKFFVCVYKS PNSNKLNAII FDKLGTSCFE
110 120 130 140 150
SVDISSNSQY YPAIENLSPS DQESNVKKCI AVILLQRYPL LSPSDLSQIL
160 170 180 190 200
SNKSENCDYD PPYAGDLASS CQLITAVPPE DLGKRFFTSG LLQNRFVSST
210 220 230 240 250
LLDVIYENNE STIELNNRLV FHLGEQLEQL FNPVTEYSPE QTEYGYKAPE
260 270 280 290 300
DELPTESDDD LVKAICNELL QLQTNFTFNL VEFLQKFLIA LRVRVLNEEI
310 320 330 340 350
NGLSTTKLNR LFPPTIDEVT RINCIFLDSL KTAIPYGSLE VLKACSITIP
360 370 380 390 400
YFYKAYTRHE AATKNFSKDI KLFIRHFSNV IPEREVYTEM KIESIIKGPQ
410 420 430 440 450
EKLLKLKLII ERLWKSKKWR PKNQEMAKKC YNNIIDVIDS FGKLDSPLHS
460 470 480 490 500
YSTRVFTPSG KILTELAKCW PVELQYKWLK RRVVGVYDVV DLNDENKRNL
510 520 530 540 550
LVIFSDYVVF INILEAESYY TSDGSNRPLI SDILMNSLIN EVPLPSKIPK
560 570 580 590 600
LKVERHCYID EVLVSILDKS TLRFDRLKGK DSFSMVCKLS SAFISSSSVA
610 620 630 640 650
DLITKARILE KDTAFHLFKA SRSHFTLYST AHELCAYDSE KIKSKFALFL
660 670 680 690 700
NIPPSKEILE VNNLHLAFFA RFCSNDGRDN IVILDVLTKH DDKHIEVTSD
710 720 730 740 750
NIVFTIINQL AIEIPICFSS LNSSMAKDLL CVNENLIKNL EHQLEEVKHP
760 770 780 790 800
STDEHRAVNS KLSGASDFDA THEKKRSYGT ITTFRSYTSD LKDSPSGDNS
810 820 830 840 850
NVTKETKEIL PVKPTKKSSK KPREIQKKTK TNASKAEHIE KKKPNKGKGF
860 870 880 890 900
FGVLKNVFGS KSKSKPSPVQ RVPKKISQRH PKSPVKKPMT SEKKSSPKRA
910 920 930 940 950
VVSSPKIKKK STSFSTKESQ TAKSSLRAVE FKSDDLIGKP PDVGNGAHPQ
960 970 980 990 1000
ENTRISSVVR DTKYVSYNPS QPVTENTSNE KNVEPKADQS TKQDNISNFA
1010 1020 1030 1040 1050
DVEVSASSYP EKLDAETDDQ IIGKATNSSS VHGNKELPDL AEVTTANRVS
1060 1070 1080 1090 1100
TTSAGDQRID TQSEFLRAAD VENLSDDDEH RQNESRVFND DLFGDFIPKH
1110 1120 1130 1140 1150
YRNKQENINS SSNLFPEGKV PQEKGVSNEN TNISLKTNED ASTLTQKLSP
1160 1170 1180 1190 1200
QASKVLTENS NELKDTNNEG KDAKDIKLGD DYSDKETAKE ITKPKNFVEG
1210 1220 1230 1240 1250
ITERKEIFPT IPRLAPPASK INFQRSPSYI ELFQGMRVVL DKHDAHYNWK
1260 1270 1280 1290 1300
RLASQVSLSE GLKVNTEEDA AIINKSQDDA KAERMTQISE VIEYEMQQPI
1310 1320 1330 1340 1350
PTYLPKAHLD DSGIEKSDDK FFEIEEELKE ELKGSKTGNE DVGNNNPSNS
1360 1370 1380 1390 1400
IPKIEKPPAF KVIRTSPVRI IGRTFEDTRK YENGSPSDIS FTYDTHNNDE
1410 1420 1430 1440 1450
PDKRLMELKF PSQDEIPDDR FYTPAEEPTA EFPVEELPNT PRSINVTTSN
1460 1470 1480 1490 1500
NKSTDDKLSS GNIDQKPTEL LDDLEFSSFN IAFGNTSMST DNMKISSDLS
1510 1520 1530 1540 1550
SNKTVLGNAQ KVQESPSGPL IYVLPQSSTK HEKEGFLRKK QKDEPIWVSP
1560 1570 1580 1590 1600
SKIDFADLSR RTKALTPERN TVPLKNNDSR KYKYTGEGSI GNMTNMLLTK
1610 1620 1630
DASYAYLKDF VALSDDEDED GKQNCAVGGP EKLKFY
Length:1,636
Mass (Da):184,719
Last modified:November 1, 1995 - v2
Checksum:i9E4E46BA5C3A3F69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17580 Genomic DNA. Translation: AAA86315.1.
X59720 Genomic DNA. Translation: CAA42346.2.
BK006937 Genomic DNA. Translation: DAA07468.1.
PIRiS74285.
S74286.
RefSeqiNP_009914.2. NM_001178662.1.

Genome annotation databases

EnsemblFungiiCAA42346; CAA42346; CAA42346.
YCL014W; YCL014W; YCL014W.
GeneIDi850345.
KEGGisce:YCL014W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17580 Genomic DNA. Translation: AAA86315.1.
X59720 Genomic DNA. Translation: CAA42346.2.
BK006937 Genomic DNA. Translation: DAA07468.1.
PIRiS74285.
S74286.
RefSeqiNP_009914.2. NM_001178662.1.

3D structure databases

ProteinModelPortaliP25558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30969. 64 interactors.
DIPiDIP-4969N.
IntActiP25558. 7 interactors.
MINTiMINT-522762.

PTM databases

iPTMnetiP25558.

Proteomic databases

MaxQBiP25558.
PRIDEiP25558.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42346; CAA42346; CAA42346.
YCL014W; YCL014W; YCL014W.
GeneIDi850345.
KEGGisce:YCL014W.

Organism-specific databases

EuPathDBiFungiDB:YCL014W.
SGDiS000000520. BUD3.

Phylogenomic databases

HOGENOMiHOG000000894.
InParanoidiP25558.
OMAiYTEMKIE.
OrthoDBiEOG092C2ME1.

Enzyme and pathway databases

BioCyciYEAST:G3O-29281-MONOMER.

Miscellaneous databases

PROiP25558.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
InterProiIPR021895. Bud3_N.
IPR000219. DH-domain.
[Graphical view]
PfamiPF12015. DUF3507. 1 hit.
[Graphical view]
SMARTiSM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBUD3_YEAST
AccessioniPrimary (citable) accession number: P25558
Secondary accession number(s): D6VQZ9
, P25556, P25557, P87007
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.