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P25558

- BUD3_YEAST

UniProt

P25558 - BUD3_YEAST

Protein

Bud site selection protein 3

Gene

BUD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Co-assembles with BUD4 at bud sites. BUD4 and BUD3 may cooperate to recognize a spatial landmark (the neck filaments) during mitosis and they subsequently become a landmark for establishing the axial budding pattern in G1.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. Rho guanyl-nucleotide exchange factor activity Source: InterPro

    GO - Biological processi

    1. axial cellular bud site selection Source: SGD

    Keywords - Biological processi

    Cell cycle

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29281-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bud site selection protein 3
    Gene namesi
    Name:BUD3
    Ordered Locus Names:YCL014W
    ORF Names:YCL012W, YCL013W, YCL12W, YCL13W, YCL14W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCL014w.
    SGDiS000000520. BUD3.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cellular bud neck contractile ring Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 16361636Bud site selection protein 3PRO_0000065016Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei766 – 7661Phosphoserine1 Publication
    Modified residuei1045 – 10451Phosphothreonine1 Publication
    Modified residuei1063 – 10631Phosphoserine2 Publications
    Modified residuei1075 – 10751Phosphoserine3 Publications
    Modified residuei1134 – 11341Phosphoserine2 Publications
    Modified residuei1149 – 11491Phosphoserine1 Publication
    Modified residuei1157 – 11571Phosphothreonine2 Publications
    Modified residuei1160 – 11601Phosphoserine2 Publications
    Modified residuei1228 – 12281Phosphoserine1 Publication
    Modified residuei1254 – 12541Phosphoserine2 Publications
    Modified residuei1257 – 12571Phosphoserine2 Publications
    Modified residuei1390 – 13901Phosphoserine1 Publication
    Modified residuei1412 – 14121Phosphoserine2 Publications
    Modified residuei1429 – 14291Phosphothreonine1 Publication
    Modified residuei1440 – 14401Phosphothreonine2 Publications
    Modified residuei1443 – 14431Phosphoserine2 Publications
    Modified residuei1501 – 15011Phosphoserine1 Publication
    Modified residuei1549 – 15491Phosphoserine1 Publication
    Modified residuei1589 – 15891Phosphoserine1 Publication
    Modified residuei1614 – 16141Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP25558.
    PaxDbiP25558.
    PeptideAtlasiP25558.

    Expressioni

    Gene expression databases

    GenevestigatoriP25558.

    Interactioni

    Subunit structurei

    Interacts with AXL2.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AXL2P389282EBI-3840,EBI-3397

    Protein-protein interaction databases

    BioGridi30969. 64 interactions.
    DIPiDIP-4969N.
    IntActiP25558. 7 interactions.
    MINTiMINT-522762.

    Structurei

    3D structure databases

    ProteinModelPortaliP25558.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the BUD3 family.Curated

    Phylogenomic databases

    eggNOGiNOG40287.
    HOGENOMiHOG000000894.
    OrthoDBiEOG72G1H3.

    Family and domain databases

    Gene3Di1.20.900.10. 1 hit.
    InterProiIPR000219. DH-domain.
    IPR021895. DUF3507.
    [Graphical view]
    PfamiPF12015. DUF3507. 1 hit.
    [Graphical view]
    SMARTiSM00325. RhoGEF. 1 hit.
    [Graphical view]
    SUPFAMiSSF48065. SSF48065. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P25558-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKDLSSLYS EKKDKENDET LFNIKLSKSV VETTPLNGHS LFDDDKSLSD     50
    WTDNVFTQSV FYHGSDDLIW GKFFVCVYKS PNSNKLNAII FDKLGTSCFE 100
    SVDISSNSQY YPAIENLSPS DQESNVKKCI AVILLQRYPL LSPSDLSQIL 150
    SNKSENCDYD PPYAGDLASS CQLITAVPPE DLGKRFFTSG LLQNRFVSST 200
    LLDVIYENNE STIELNNRLV FHLGEQLEQL FNPVTEYSPE QTEYGYKAPE 250
    DELPTESDDD LVKAICNELL QLQTNFTFNL VEFLQKFLIA LRVRVLNEEI 300
    NGLSTTKLNR LFPPTIDEVT RINCIFLDSL KTAIPYGSLE VLKACSITIP 350
    YFYKAYTRHE AATKNFSKDI KLFIRHFSNV IPEREVYTEM KIESIIKGPQ 400
    EKLLKLKLII ERLWKSKKWR PKNQEMAKKC YNNIIDVIDS FGKLDSPLHS 450
    YSTRVFTPSG KILTELAKCW PVELQYKWLK RRVVGVYDVV DLNDENKRNL 500
    LVIFSDYVVF INILEAESYY TSDGSNRPLI SDILMNSLIN EVPLPSKIPK 550
    LKVERHCYID EVLVSILDKS TLRFDRLKGK DSFSMVCKLS SAFISSSSVA 600
    DLITKARILE KDTAFHLFKA SRSHFTLYST AHELCAYDSE KIKSKFALFL 650
    NIPPSKEILE VNNLHLAFFA RFCSNDGRDN IVILDVLTKH DDKHIEVTSD 700
    NIVFTIINQL AIEIPICFSS LNSSMAKDLL CVNENLIKNL EHQLEEVKHP 750
    STDEHRAVNS KLSGASDFDA THEKKRSYGT ITTFRSYTSD LKDSPSGDNS 800
    NVTKETKEIL PVKPTKKSSK KPREIQKKTK TNASKAEHIE KKKPNKGKGF 850
    FGVLKNVFGS KSKSKPSPVQ RVPKKISQRH PKSPVKKPMT SEKKSSPKRA 900
    VVSSPKIKKK STSFSTKESQ TAKSSLRAVE FKSDDLIGKP PDVGNGAHPQ 950
    ENTRISSVVR DTKYVSYNPS QPVTENTSNE KNVEPKADQS TKQDNISNFA 1000
    DVEVSASSYP EKLDAETDDQ IIGKATNSSS VHGNKELPDL AEVTTANRVS 1050
    TTSAGDQRID TQSEFLRAAD VENLSDDDEH RQNESRVFND DLFGDFIPKH 1100
    YRNKQENINS SSNLFPEGKV PQEKGVSNEN TNISLKTNED ASTLTQKLSP 1150
    QASKVLTENS NELKDTNNEG KDAKDIKLGD DYSDKETAKE ITKPKNFVEG 1200
    ITERKEIFPT IPRLAPPASK INFQRSPSYI ELFQGMRVVL DKHDAHYNWK 1250
    RLASQVSLSE GLKVNTEEDA AIINKSQDDA KAERMTQISE VIEYEMQQPI 1300
    PTYLPKAHLD DSGIEKSDDK FFEIEEELKE ELKGSKTGNE DVGNNNPSNS 1350
    IPKIEKPPAF KVIRTSPVRI IGRTFEDTRK YENGSPSDIS FTYDTHNNDE 1400
    PDKRLMELKF PSQDEIPDDR FYTPAEEPTA EFPVEELPNT PRSINVTTSN 1450
    NKSTDDKLSS GNIDQKPTEL LDDLEFSSFN IAFGNTSMST DNMKISSDLS 1500
    SNKTVLGNAQ KVQESPSGPL IYVLPQSSTK HEKEGFLRKK QKDEPIWVSP 1550
    SKIDFADLSR RTKALTPERN TVPLKNNDSR KYKYTGEGSI GNMTNMLLTK 1600
    DASYAYLKDF VALSDDEDED GKQNCAVGGP EKLKFY 1636
    Length:1,636
    Mass (Da):184,719
    Last modified:November 1, 1995 - v2
    Checksum:i9E4E46BA5C3A3F69
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17580 Genomic DNA. Translation: AAA86315.1.
    X59720 Genomic DNA. Translation: CAA42346.2.
    BK006937 Genomic DNA. Translation: DAA07468.1.
    PIRiS74285.
    S74286.
    RefSeqiNP_009914.2. NM_001178662.1.

    Genome annotation databases

    EnsemblFungiiYCL014W; YCL014W; YCL014W.
    GeneIDi850345.
    KEGGisce:YCL014W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17580 Genomic DNA. Translation: AAA86315.1 .
    X59720 Genomic DNA. Translation: CAA42346.2 .
    BK006937 Genomic DNA. Translation: DAA07468.1 .
    PIRi S74285.
    S74286.
    RefSeqi NP_009914.2. NM_001178662.1.

    3D structure databases

    ProteinModelPortali P25558.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30969. 64 interactions.
    DIPi DIP-4969N.
    IntActi P25558. 7 interactions.
    MINTi MINT-522762.

    Proteomic databases

    MaxQBi P25558.
    PaxDbi P25558.
    PeptideAtlasi P25558.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCL014W ; YCL014W ; YCL014W .
    GeneIDi 850345.
    KEGGi sce:YCL014W.

    Organism-specific databases

    CYGDi YCL014w.
    SGDi S000000520. BUD3.

    Phylogenomic databases

    eggNOGi NOG40287.
    HOGENOMi HOG000000894.
    OrthoDBi EOG72G1H3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29281-MONOMER.

    Miscellaneous databases

    NextBioi 965797.

    Gene expression databases

    Genevestigatori P25558.

    Family and domain databases

    Gene3Di 1.20.900.10. 1 hit.
    InterProi IPR000219. DH-domain.
    IPR021895. DUF3507.
    [Graphical view ]
    Pfami PF12015. DUF3507. 1 hit.
    [Graphical view ]
    SMARTi SM00325. RhoGEF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48065. SSF48065. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Role of Bud3p in producing the axial budding pattern of yeast."
      Chant J., Mischke M., Mitchell E., Herskowitz I., Pringle J.R.
      J. Cell Biol. 129:767-778(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Gromadka R.
      Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    4. Valles G., Volckaerts G.
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1075 AND SER-1614, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle."
      Gao X.D., Sperber L.M., Kane S.A., Tong Z., Tong A.H., Boone C., Bi E.
      Mol. Biol. Cell 18:2542-2560(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AXL2.
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1063; SER-1075; SER-1134; SER-1149; THR-1157; SER-1160; SER-1254; SER-1257; SER-1412; THR-1440; SER-1443 AND SER-1614, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-766; THR-1045; SER-1063; SER-1075; SER-1134; THR-1157; SER-1160; SER-1228; SER-1254; SER-1257; SER-1390; SER-1412; THR-1429; THR-1440; SER-1443; SER-1501; SER-1549; SER-1589 AND SER-1614, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBUD3_YEAST
    AccessioniPrimary (citable) accession number: P25558
    Secondary accession number(s): D6VQZ9
    , P25556, P25557, P87007
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3