P25555 (GBP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Single-strand telomeric DNA-binding protein GBP2
Short name=G-strand-binding protein 2

Alternative name(s):
RAP1 localization factor 6

Gene names

Name:	GBP2
Synonyms:	RLF6
Ordered Locus Names:	YCL011C
ORF Names:	YCL11C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds single-stranded telomeric sequences of the type (TG[1-3])n in vitro. Also binds to RNA. Influences the localization of RAP1 in the nuclei. Involved in modulating telomere length.
Subcellular location	Nucleus Potential. Chromosome › telomere.
Miscellaneous	Present with 2540 molecules/cell in log phase SD medium.
Sequence similarities	Contains 3 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Cellular component	Chromosome Nucleus Telomere
Domain	Repeat
Ligand	DNA-binding RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	poly(A)+ mRNA export from nucleus Inferred from genetic interaction PubMed 12634846. Source: SGD telomere maintenance Inferred from direct assay Ref.6. Source: SGD
Cellular_component	chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic stress granule Inferred from direct assay PubMed 18981231. Source: SGD nucleus Inferred from direct assay PubMed 12634846. Source: SGD
Molecular_function	RNA binding Inferred from direct assay PubMed 12634846. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro telomeric DNA binding Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Single-strand telomeric DNA-binding protein GBP2		PRO_0000081596
Regions
	Domain	122 – 198	77	RRM 1
	Domain	219 – 296	78	RRM 2
	Domain	349 – 426	78	RRM 3
Amino acid modifications
	Modified residue	24	1	Phosphoserine Ref.8
	Modified residue	130	1	Phosphothreonine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P25555 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 5341D7A0E07F208C	FASTA	427	48,729
	10 20 30 40 50 60 MERELGMYGN DRSRSRSPVR RRLSDDRDRY DDYNDSSSNN GNGSRRQRRD RGSRFNDRYD 70 80 90 100 110 120 QSYGGSRYHD DRNWPPRRGG RGRGGSRSFR GGRGGGRGRT LGPIVERDLE RQFDATKRNF 130 140 150 160 170 180 ENSIFVRNLT FDCTPEDLKE LFGTVGEVVE ADIITSKGHH RGMGTVEFTK NESVQDAISK 190 200 210 220 230 240 FDGALFMDRK LMVRQDNPPP EAAKEFSKKA TREEIDNGFE VFIINLPYSM NWQSLKDMFK 250 260 270 280 290 300 ECGHVLRADV ELDFNGFSRG FGSVIYPTED EMIRAIDTFN GMEVEGRVLE VREGRFNKRK 310 320 330 340 350 360 NNDRYNQRRE DLEDTRGTEP GLAQDAAVHI DETAAKFTEG VNPGGDRNCF IYCSNLPFST 370 380 390 400 410 420 ARSDLFDLFG PIGKINNAEL KPQENGQPTG VAVVEYENLV DADFCIQKLN NYNYGGCSLQ ISYARRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Isolation and characterization of two Saccharomyces cerevisiae genes that encode proteins that bind to (TG1-3)n single strand telomeric DNA in vitro." Lin J.-J., Zakian V.A. Nucleic Acids Res. 22:4906-4913(1994) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"A class of single-stranded telomeric DNA-binding proteins required for Rap1p localization in yeast nuclei." Konkel L.C., Enomoto S., Chamberlain E., McCune-Zierath P., Iyadurai S.J.P., Berman J. Proc. Natl. Acad. Sci. U.S.A. 92:5558-5562(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Exposure of single-stranded telomeric DNA causes G2/M cell cycle arrest in Saccharomyces cerevisiae." Pang T.-L., Wang C.-Y., Hsu C.-L., Chen M.-Y., Lin J.-J. J. Biol. Chem. 278:9318-9321(2003) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-130, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59720 Genomic DNA. Translation: CAA42348.1. AY692807 Genomic DNA. Translation: AAT92826.1. BK006937 Genomic DNA. Translation: DAA07470.1.
PIR	S19338.
RefSeq	NP_009916.1. NM_001178660.1.
3D structure databases
ProteinModelPortal	P25555.
SMR	P25555. Positions 122-296, 346-427.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2735N.
IntAct	P25555. 50 interactions.
MINT	MINT-481140.
STRING	4932.YCL011C.
Proteomic databases
PaxDb	P25555.
PeptideAtlas	P25555.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCL011C; YCL011C; YCL011C.
GeneID	850346.
KEGG	sce:YCL011C.
Organism-specific databases
CYGD	YCL011c.
SGD	S000000517. GBP2.
Phylogenomic databases
eggNOG	COG0724.
GeneTree	ENSGT00410000025635.
HOGENOM	HOG000111155.
OMA	ATHENTE.
OrthoDB	EOG4F21C2.
Gene expression databases
Genevestigator	P25555.
GermOnline	YCL011C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.70.330. 3 hits.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00076. RRM_1. 3 hits. [Graphical view]
SMART	SM00360. RRM. 3 hits. [Graphical view]
PROSITE	PS50102. RRM. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	965800.

Entry information

Entry name

GBP2_YEAST

Accession

Primary (citable) accession number: P25555
Secondary accession number(s): D6VR01

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 1, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program