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Protein

Single-strand telomeric DNA-binding protein GBP2

Gene

GBP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds single-stranded telomeric sequences of the type (TG[1-3])n in vitro. Also binds to RNA. Influences the localization of RAP1 in the nuclei. Involved in modulating telomere length.

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. RNA binding Source: SGD
  3. telomeric DNA binding Source: SGD

GO - Biological processi

  1. nuclear mRNA surveillance Source: SGD
  2. poly(A)+ mRNA export from nucleus Source: SGD
  3. telomere maintenance Source: SGD
Complete GO annotation...

Keywords - Ligandi

DNA-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29280-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Single-strand telomeric DNA-binding protein GBP2
Short name:
G-strand-binding protein 2
Alternative name(s):
RAP1 localization factor 6
Gene namesi
Name:GBP2
Synonyms:RLF6
Ordered Locus Names:YCL011C
ORF Names:YCL11C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome III

Organism-specific databases

CYGDiYCL011c.
SGDiS000000517. GBP2.

Subcellular locationi

GO - Cellular componenti

  1. chromosome, telomeric region Source: UniProtKB-SubCell
  2. cytoplasmic stress granule Source: SGD
  3. cytosol Source: SGD
  4. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Single-strand telomeric DNA-binding protein GBP2PRO_0000081596Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei130 – 1301Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25555.
PaxDbiP25555.
PeptideAtlasiP25555.

Expressioni

Gene expression databases

GenevestigatoriP25555.

Interactioni

Protein-protein interaction databases

BioGridi30970. 124 interactions.
DIPiDIP-2735N.
IntActiP25555. 46 interactions.
MINTiMINT-481140.
STRINGi4932.YCL011C.

Structurei

3D structure databases

SMRiP25555. Positions 122-300, 347-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini122 – 19877RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini219 – 29678RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini349 – 42678RRM 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00410000025635.
HOGENOMiHOG000111155.
InParanoidiP25555.
OMAiREIFVRQ.
OrthoDBiEOG76TB3G.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25555-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERELGMYGN DRSRSRSPVR RRLSDDRDRY DDYNDSSSNN GNGSRRQRRD
60 70 80 90 100
RGSRFNDRYD QSYGGSRYHD DRNWPPRRGG RGRGGSRSFR GGRGGGRGRT
110 120 130 140 150
LGPIVERDLE RQFDATKRNF ENSIFVRNLT FDCTPEDLKE LFGTVGEVVE
160 170 180 190 200
ADIITSKGHH RGMGTVEFTK NESVQDAISK FDGALFMDRK LMVRQDNPPP
210 220 230 240 250
EAAKEFSKKA TREEIDNGFE VFIINLPYSM NWQSLKDMFK ECGHVLRADV
260 270 280 290 300
ELDFNGFSRG FGSVIYPTED EMIRAIDTFN GMEVEGRVLE VREGRFNKRK
310 320 330 340 350
NNDRYNQRRE DLEDTRGTEP GLAQDAAVHI DETAAKFTEG VNPGGDRNCF
360 370 380 390 400
IYCSNLPFST ARSDLFDLFG PIGKINNAEL KPQENGQPTG VAVVEYENLV
410 420
DADFCIQKLN NYNYGGCSLQ ISYARRD
Length:427
Mass (Da):48,729
Last modified:May 1, 1992 - v1
Checksum:i5341D7A0E07F208C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42348.1.
AY692807 Genomic DNA. Translation: AAT92826.1.
BK006937 Genomic DNA. Translation: DAA07470.1.
PIRiS19338.
RefSeqiNP_009916.1. NM_001178660.1.

Genome annotation databases

EnsemblFungiiYCL011C; YCL011C; YCL011C.
GeneIDi850346.
KEGGisce:YCL011C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42348.1.
AY692807 Genomic DNA. Translation: AAT92826.1.
BK006937 Genomic DNA. Translation: DAA07470.1.
PIRiS19338.
RefSeqiNP_009916.1. NM_001178660.1.

3D structure databases

SMRiP25555. Positions 122-300, 347-427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30970. 124 interactions.
DIPiDIP-2735N.
IntActiP25555. 46 interactions.
MINTiMINT-481140.
STRINGi4932.YCL011C.

Proteomic databases

MaxQBiP25555.
PaxDbiP25555.
PeptideAtlasiP25555.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYCL011C; YCL011C; YCL011C.
GeneIDi850346.
KEGGisce:YCL011C.

Organism-specific databases

CYGDiYCL011c.
SGDiS000000517. GBP2.

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00410000025635.
HOGENOMiHOG000111155.
InParanoidiP25555.
OMAiREIFVRQ.
OrthoDBiEOG76TB3G.

Enzyme and pathway databases

BioCyciYEAST:G3O-29280-MONOMER.

Miscellaneous databases

NextBioi965800.

Gene expression databases

GenevestigatoriP25555.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Isolation and characterization of two Saccharomyces cerevisiae genes that encode proteins that bind to (TG1-3)n single strand telomeric DNA in vitro."
    Lin J.-J., Zakian V.A.
    Nucleic Acids Res. 22:4906-4913(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "A class of single-stranded telomeric DNA-binding proteins required for Rap1p localization in yeast nuclei."
    Konkel L.C., Enomoto S., Chamberlain E., McCune-Zierath P., Iyadurai S.J.P., Berman J.
    Proc. Natl. Acad. Sci. U.S.A. 92:5558-5562(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Exposure of single-stranded telomeric DNA causes G2/M cell cycle arrest in Saccharomyces cerevisiae."
    Pang T.-L., Wang C.-Y., Hsu C.-L., Chen M.-Y., Lin J.-J.
    J. Biol. Chem. 278:9318-9321(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-130, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGBP2_YEAST
AccessioniPrimary (citable) accession number: P25555
Secondary accession number(s): D6VR01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 1, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2540 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.