Lactaldehyde dehydrogenase - Escherichia coli (strain K12)

Names and origin

Protein names

Recommended name:
    Lactaldehyde dehydrogenase
    EC=1.2.1.22
Alternative name(s):
    Glycolaldehyde dehydrogenase
    EC=1.2.1.21
    Aldehyde dehydrogenase A

Gene names

Name:	aldA
Synonyms:	ald
Ordered Locus Names:	b1415, JW1412

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Acts on lactaldehyde as well as other aldehydes.
Catalytic activity	(S)-lactaldehyde + NAD⁺ + H₂O = (S)-lactate + NADH. Glycolaldehyde + NAD⁺ + H₂O = glycolate + NADH.
Subunit structure	Homotetramer.
Induction	By growth on fucose, rhamnose, arabinose and amino acids such as glutamate.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

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Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycolaldehyde dehydrogenase activity Inferred from electronic annotation. Source: EC lactaldehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

Removed Ref.1 Ref.5

Chain

2 – 479

478

Lactaldehyde dehydrogenase

PRO_0000056564

Regions

Nucleotide binding

207 – 213

7

NAD By similarity

Sites

Active site

251

1

By similarity

Active site

285

1

By similarity

Secondary structure

1

.

479

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P25553-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: DA7819EA0C05C32F
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FASTA

479

52,273

        10         20         30         40         50         60 
MSVPVQHPMY IDGQFVTWRG DAWIDVVNPA TEAVISRIPD GQAEDARKAI DAAERAQPEW 

        70         80         90        100        110        120 
EALPAIERAS WLRKISAGIR ERASEISALI VEEGGKIQQL AEVEVAFTAD YIDYMAEWAR 

       130        140        150        160        170        180 
RYEGEIIQSD RPGENILLFK RALGVTTGIL PWNFPFFLIA RKMAPALLTG NTIVIKPSEF 

       190        200        210        220        230        240 
TPNNAIAFAK IVDEIGLPRG VFNLVLGRGE TVGQELAGNP KVAMVSMTGS VSAGEKIMAT 

       250        260        270        280        290        300 
AAKNITKVCL ELGGKAPAIV MDDADLELAV KAIVDSRVIN SGQVCNCAER VYVQKGIYDQ 

       310        320        330        340        350        360 
FVNRLGEAMQ AVQFGNPAER NDIAMGPLIN AAALERVEQK VARAVEEGAR VAFGGKAVEG 

       370        380        390        400        410        420 
KGYYYPPTLL LDVRQEMSIM HEETFGPVLP VVAFDTLEDA ISMANDSDYG LTSSIYTQNL 

       430        440        450        460        470 
NVAMKAIKGL KFGETYINRE NFEAMQGFHA GWRKSGIGGA DGKHGLHEYL QTQVVYLQS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and DNA sequencing of the Escherichia coli K-12 ald gene encoding aldehyde dehydrogenase." Hidalgo E., Chen Y.-M., Lin E.C.C., Aguilar J. J. Bacteriol. 173:6118-6123(1991) [PubMed: 1917845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-12. Strain: K12 / EMG2.
[6]	"Identification of lactaldehyde dehydrogenase and glycolaldehyde dehydrogenase as functions of the same protein in Escherichia coli." Caballero E., Baldoma L., Ros J., Boronat A., Aguilar J. J. Biol. Chem. 258:7788-7792(1983) [PubMed: 6345530] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Involvement of lactaldehyde dehydrogenase in several metabolic pathways of Escherichia coli K12." Baldoma L., Aguilar J. J. Biol. Chem. 262:13991-13996(1987) [PubMed: 3308886] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

M64541 Genomic DNA. Translation: AAA23427.1.
U00096 Genomic DNA. Translation: AAC74497.1.
AP009048 Genomic DNA. Translation: BAA15032.1.

PIR

A38165.

RefSeq

AP_002040.1.
NP_415933.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HG2	X-ray	2.20	A	2-478	[»]
2ILU	X-ray	2.70	A	2-478	[»]
2IMP	X-ray	2.10	A	2-478	[»]
2OPX	X-ray	2.53	A	1-479	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:9081N.

2-D gel databases

SWISS-2DPAGE

P25553.

Genome annotation databases

GeneID

945672.

GenomeReviews

Gene locus JW1412 in contig AP009048_GR.
Gene locus b1415 in contig U00096_GR.

KEGG

ecj:JW1412.
eco:b1415.

Organism-specific databases

EchoBASE

EB0034.

EcoGene

EG10035. aldA.

CMR

Search...

Phylogenomic databases

HOGENOM

P25553.

OMA

P25553. GENILVF.

Enzyme and pathway databases

BioCyc

EcoCyc:LACTALDDEHYDROG-MON.
MetaCyc:LACTALDDEHYDROG-MON.

BRENDA

1.2.1.22. 246.

Family and domain databases

InterPro

IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH.
[Graphical view]

Gene3D

G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.

PANTHER

PTHR11699. Aldehyde_dehyd. 1 hit.

Pfam

PF00171. Aldedh. 1 hit.
[Graphical view]

PROSITE

PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

ALDA_ECOLI

Accession

Primary (citable) accession number: P25553

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families