Lactaldehyde dehydrogenase - Escherichia coli (strain K12)

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P25553 (ALDA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Lactaldehyde dehydrogenase
EC=1.2.1.22

Alternative name(s):
Aldehyde dehydrogenase A
Glycolaldehyde dehydrogenase
EC=1.2.1.21

Gene names

Name:	aldA
Synonyms:	ald
Ordered Locus Names:	b1415, JW1412

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts on lactaldehyde as well as other aldehydes.
Catalytic activity	(S)-lactaldehyde + NAD⁺ + H₂O = (S)-lactate + NADH. Glycolaldehyde + NAD⁺ + H₂O = glycolate + NADH.
Subunit structure	Homotetramer.
Induction	By growth on fucose, rhamnose, arabinose and amino acids such as glutamate.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	D-arabinose catabolic process Non-traceable author statement. Source: EcoCyc L-fucose catabolic process Inferred from expression pattern. Source: EcoCyc rhamnose catabolic process Inferred from mutant phenotype. Source: EcoCyc
Molecular function	glycolaldehyde dehydrogenase activity Inferred from direct assay Ref.7. Source: EcoCyc lactaldehyde dehydrogenase activity Inferred from direct assay Ref.7. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.1 Ref.5

Chain

2 – 479

478

Lactaldehyde dehydrogenase

PRO_0000056564

Regions

Nucleotide binding

207 – 213

NAD By similarity

Sites

Active site

251

By similarity

Active site

285

By similarity

Secondary structure

479

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P25553 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: DA7819EA0C05C32F
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FASTA

479

52,273

        10         20         30         40         50         60 
MSVPVQHPMY IDGQFVTWRG DAWIDVVNPA TEAVISRIPD GQAEDARKAI DAAERAQPEW 

        70         80         90        100        110        120 
EALPAIERAS WLRKISAGIR ERASEISALI VEEGGKIQQL AEVEVAFTAD YIDYMAEWAR 

       130        140        150        160        170        180 
RYEGEIIQSD RPGENILLFK RALGVTTGIL PWNFPFFLIA RKMAPALLTG NTIVIKPSEF 

       190        200        210        220        230        240 
TPNNAIAFAK IVDEIGLPRG VFNLVLGRGE TVGQELAGNP KVAMVSMTGS VSAGEKIMAT 

       250        260        270        280        290        300 
AAKNITKVCL ELGGKAPAIV MDDADLELAV KAIVDSRVIN SGQVCNCAER VYVQKGIYDQ 

       310        320        330        340        350        360 
FVNRLGEAMQ AVQFGNPAER NDIAMGPLIN AAALERVEQK VARAVEEGAR VAFGGKAVEG 

       370        380        390        400        410        420 
KGYYYPPTLL LDVRQEMSIM HEETFGPVLP VVAFDTLEDA ISMANDSDYG LTSSIYTQNL 

       430        440        450        460        470 
NVAMKAIKGL KFGETYINRE NFEAMQGFHA GWRKSGIGGA DGKHGLHEYL QTQVVYLQS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and DNA sequencing of the Escherichia coli K-12 ald gene encoding aldehyde dehydrogenase." Hidalgo E., Chen Y.-M., Lin E.C.C., Aguilar J. J. Bacteriol. 173:6118-6123(1991) [PubMed: 1917845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-12. Strain: K12 / EMG2.
[6]	"Identification of lactaldehyde dehydrogenase and glycolaldehyde dehydrogenase as functions of the same protein in Escherichia coli." Caballero E., Baldoma L., Ros J., Boronat A., Aguilar J. J. Biol. Chem. 258:7788-7792(1983) [PubMed: 6345530] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Involvement of lactaldehyde dehydrogenase in several metabolic pathways of Escherichia coli K12." Baldoma L., Aguilar J. J. Biol. Chem. 262:13991-13996(1987) [PubMed: 3308886] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M64541 Genomic DNA. Translation: AAA23427.1.
U00096 Genomic DNA. Translation: AAC74497.1.
AP009048 Genomic DNA. Translation: BAA15032.1.

PIR

A38165.

RefSeq

NP_415933.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HG2	X-ray	2.20	A	2-478	[»]
2ILU	X-ray	2.70	A	2-478	[»]
2IMP	X-ray	2.10	A	2-478	[»]
2OPX	X-ray	2.53	A	1-479	[»]

ProteinModelPortal

P25553.

SMR

P25553. Positions 3-479.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-9081N.

IntAct

P25553. 5 interactions.

MINT

MINT-1313045.

2D gel databases

SWISS-2DPAGE

P25553.

Proteomic databases

PRIDE

P25553.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000003609; EBESCP00000003609; EBESCG00000002944.
EBESCT00000018440; EBESCP00000017731; EBESCG00000017494.

GeneID

945672.

GenomeReviews

Gene locus JW1412 in contig AP009048_GR.
Gene locus b1415 in contig U00096_GR.

KEGG

ecj:JW1412.
eco:b1415.

PATRIC

32118116. VBIEscCol129921_1478.

Organism-specific databases

EchoBASE

EB0034.

EcoGene

EG10035. aldA.

Phylogenomic databases

eggNOG

COG1012.

GeneTree

EBGT00050000009175.

HOGENOM

HBG752218.

OMA

GENILVF.

PhylomeDB

P25553.

ProtClustDB

PRK10090.

Enzyme and pathway databases

BioCyc

EcoCyc:LACTALDDEHYDROG-MONOMER.
MetaCyc:LACTALDDEHYDROG-MONOMER.

BRENDA

1.2.1.22. 2026.

Gene expression databases

Genevestigator

P25553.

Family and domain databases

InterPro

IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]

Gene3D

G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit.
G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.

K07248.

Pfam

PF00171. Aldedh. 1 hit.
[Graphical view]

SUPFAM

SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.

PROSITE

PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ALDA_ECOLI

Accession

Primary (citable) accession number: P25553

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents