Reviewed, UniProtKB/Swiss-Prot P25552 (GPPA_ECOLI)

Names and origin

Protein names

Recommended name:
    Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
    EC=3.6.1.40
Alternative name(s):
    Guanosine pentaphosphate phosphohydrolase
    pppGpp-5'-phosphohydrolase

Gene names

Name:	gppA
Synonyms:	gpp
Ordered Locus Names:	b3779, JW5603

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities. HAMAP MF_01550
Catalytic activity	Guanosine 5'-triphosphate,3'-diphosphate + H2O = guanosine 5'-diphosphate,3'-diphosphate + phosphate. HAMAP MF_01550
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2. HAMAP MF_01550
Sequence similarities	Belongs to the gppA/ppx family. GppA subfamily.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	guanosine-5'-triphosphate,3'-diphosphate diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase HAMAP MF_01550		PRO_0000194280
Experimental info
	Sequence conflict	86	1	T → R in AAA67580. Ref.2
	Sequence conflict	423	1	V → L in AAA67580. Ref.2
	Sequence conflict	437 – 494	58	FASRR…PLEVH → VGQPSP in AAB59049. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P25552-1 [UniParc]. Last modified October 11, 2004. Version 3. Checksum: C4DD8DC59432A5F9	FASTA	494	54,871
	10 20 30 40 50 60 MGSTSSLYAA IDLGSNSFHM LVVREVAGSI QTLTRIKRKV RLAAGLNSEN ALSNEAMERG 70 80 90 100 110 120 WQCLRLFAER LQDIPPSQIR VVATATLRLA VNAGDFIAKA QEILGCPVQV ISGEEEARLI 130 140 150 160 170 180 YQGVAHTTGG ADQRLVVDIG GASTELVTGT GAQTTSLFSL SMGCVTWLER YFADRNLGQE 190 200 210 220 230 240 NFDAAEKAAR EVLRPVADEL RYHGWKVCVG ASGTVQALQE IMMAQGMDER ITLEKLQQLK 250 260 270 280 290 300 QRAIHCGRLE ELEIDGLTLE RALVFPSGLA ILIAIFTELN IQCMTLAGGA LREGLVYGML 310 320 330 340 350 360 HLAVEQDIRS RTLRNIQRRF MIDIDQAQRV AKVAANFFDQ VENEWHLEAI SRDLLISACQ 370 380 390 400 410 420 LHEIGLSVDF KQAPQHAAYL VRNLDLPGFT PAQKKLLATL LLNQTNPVDL SSLHQQNAVP 430 440 450 460 470 480 PRVAEQLCRL LRLAIIFASR RRDDLVPEMT LQANHELLTL TLPQGWLTQH PLGKEIIAQE 490 SQWQSYVHWP LEVH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"rhlB, a new Escherichia coli K-12 gene with an RNA helicase-like protein sequence motif, one of at least five such possible genes in a prokaryote." Kalman M., Murphy H., Cashel M. New Biol. 3:886-895(1991) [PubMed: 1931833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R. Science 257:771-778(1992) [PubMed: 1379743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed: 16397293] [Abstract] Cited for: SEQUENCE REVISION TO 86 AND 423.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Exopolyphosphate phosphatase and guanosine pentaphosphate phosphatase belong to the sugar kinase/actin/hsp 70 superfamily." Reizer J., Reizer A., Saier M.H. Jr., Bork B., Sander C. Trends Biochem. Sci. 18:247-248(1993) [PubMed: 8212131] [Abstract] Cited for: SIMILARITY TO PPX.
[7]	"Guanosine pentaphosphate phosphohydrolase of Escherichia coli is a long-chain exopolyphosphatase." Keasling J.D., Bertsch L., Kornberg A. Proc. Natl. Acad. Sci. U.S.A. 90:7029-7033(1993) [PubMed: 8394006] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M83316 Genomic DNA. Translation: AAB59049.1. M87049 Genomic DNA. Translation: AAA67580.1. U00096 Genomic DNA. Translation: AAT48210.1. AP009048 Genomic DNA. Translation: BAE77519.1.
PIR	A48285.
RefSeq	AP_004018.1. YP_026252.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	948291.
GenomeReviews	Gene locus JW5603 in contig AP009048_GR. Gene locus b3779 in contig U00096_GR.
KEGG	ecj:JW5603. eco:b3779.
Organism-specific databases
EchoBASE	EB0408.
EcoGene	EG10413. gpp.
CMR	Search...
Phylogenomic databases
HOGENOM	P25552.
OMA	P25552. QYTDLGW.
Enzyme and pathway databases
BioCyc	EcoCyc:PPPGPPHYDRO-MON. MetaCyc:PPPGPPHYDRO-MON.
Family and domain databases
HAMAP	MF_01550. [Tree]
InterPro	IPR003695. Ppx_GppA. [Graphical view]
Pfam	PF02541. Ppx-GppA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPPA_ECOLI

Accession

Primary (citable) accession number: P25552
Secondary accession number(s): Q2M887, Q6BF04

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)