Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P25552

- GPPA_ECOLI

UniProt

P25552 - GPPA_ECOLI

Protein

Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase

Gene

gppA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 3 (11 Oct 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities.

    Catalytic activityi

    Guanosine 5'-triphosphate,3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate.

    Pathwayi

    GO - Molecular functioni

    1. guanosine-5'-triphosphate,3'-diphosphate diphosphatase activity Source: EcoCyc

    GO - Biological processi

    1. guanosine pentaphosphate catabolic process Source: InterPro
    2. guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway
    3. nucleobase-containing small molecule interconversion Source: EcoCyc
    4. phosphorus metabolic process Source: EcoCyc
    5. response to starvation Source: EcoCyc

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:PPPGPPHYDRO-MONOMER.
    ECOL316407:JW5603-MONOMER.
    MetaCyc:PPPGPPHYDRO-MONOMER.
    UniPathwayiUPA00908; UER00884.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (EC:3.6.1.40)
    Alternative name(s):
    Guanosine pentaphosphate phosphohydrolase
    pppGpp-5'-phosphohydrolase
    Gene namesi
    Name:gppA
    Synonyms:gpp
    Ordered Locus Names:b3779, JW5603
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10413. gpp.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatasePRO_0000194280Add
    BLAST

    Proteomic databases

    PaxDbiP25552.
    PRIDEiP25552.

    Expressioni

    Gene expression databases

    GenevestigatoriP25552.

    Interactioni

    Protein-protein interaction databases

    STRINGi511145.b3779.

    Structurei

    3D structure databases

    ProteinModelPortaliP25552.
    SMRiP25552. Positions 8-493.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GppA/Ppx family. GppA subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0248.
    HOGENOMiHOG000258672.
    KOiK01524.
    OMAiGLHIEYK.
    OrthoDBiEOG6M6JN6.
    PhylomeDBiP25552.

    Family and domain databases

    HAMAPiMF_01550. GppA.
    InterProiIPR023709. Guo-5TP_3DP_PyrP.
    IPR003695. Ppx_GppA.
    [Graphical view]
    PfamiPF02541. Ppx-GppA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25552-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSTSSLYAA IDLGSNSFHM LVVREVAGSI QTLTRIKRKV RLAAGLNSEN    50
    ALSNEAMERG WQCLRLFAER LQDIPPSQIR VVATATLRLA VNAGDFIAKA 100
    QEILGCPVQV ISGEEEARLI YQGVAHTTGG ADQRLVVDIG GASTELVTGT 150
    GAQTTSLFSL SMGCVTWLER YFADRNLGQE NFDAAEKAAR EVLRPVADEL 200
    RYHGWKVCVG ASGTVQALQE IMMAQGMDER ITLEKLQQLK QRAIHCGRLE 250
    ELEIDGLTLE RALVFPSGLA ILIAIFTELN IQCMTLAGGA LREGLVYGML 300
    HLAVEQDIRS RTLRNIQRRF MIDIDQAQRV AKVAANFFDQ VENEWHLEAI 350
    SRDLLISACQ LHEIGLSVDF KQAPQHAAYL VRNLDLPGFT PAQKKLLATL 400
    LLNQTNPVDL SSLHQQNAVP PRVAEQLCRL LRLAIIFASR RRDDLVPEMT 450
    LQANHELLTL TLPQGWLTQH PLGKEIIAQE SQWQSYVHWP LEVH 494
    Length:494
    Mass (Da):54,871
    Last modified:October 11, 2004 - v3
    Checksum:iC4DD8DC59432A5F9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti86 – 861T → R in AAA67580. (PubMed:1379743)Curated
    Sequence conflicti423 – 4231V → L in AAA67580. (PubMed:1379743)Curated
    Sequence conflicti437 – 49458FASRR…PLEVH → VGQPSP in AAB59049. (PubMed:1931833)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M83316 Genomic DNA. Translation: AAB59049.1.
    M87049 Genomic DNA. Translation: AAA67580.1.
    U00096 Genomic DNA. Translation: AAT48210.1.
    AP009048 Genomic DNA. Translation: BAE77519.1.
    PIRiA48285.
    RefSeqiYP_026252.1. NC_000913.3.
    YP_491660.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAT48210; AAT48210; b3779.
    BAE77519; BAE77519; BAE77519.
    GeneIDi12934325.
    948291.
    KEGGiecj:Y75_p3396.
    eco:b3779.
    PATRICi32123049. VBIEscCol129921_3894.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M83316 Genomic DNA. Translation: AAB59049.1 .
    M87049 Genomic DNA. Translation: AAA67580.1 .
    U00096 Genomic DNA. Translation: AAT48210.1 .
    AP009048 Genomic DNA. Translation: BAE77519.1 .
    PIRi A48285.
    RefSeqi YP_026252.1. NC_000913.3.
    YP_491660.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P25552.
    SMRi P25552. Positions 8-493.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 511145.b3779.

    Proteomic databases

    PaxDbi P25552.
    PRIDEi P25552.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT48210 ; AAT48210 ; b3779 .
    BAE77519 ; BAE77519 ; BAE77519 .
    GeneIDi 12934325.
    948291.
    KEGGi ecj:Y75_p3396.
    eco:b3779.
    PATRICi 32123049. VBIEscCol129921_3894.

    Organism-specific databases

    EchoBASEi EB0408.
    EcoGenei EG10413. gpp.

    Phylogenomic databases

    eggNOGi COG0248.
    HOGENOMi HOG000258672.
    KOi K01524.
    OMAi GLHIEYK.
    OrthoDBi EOG6M6JN6.
    PhylomeDBi P25552.

    Enzyme and pathway databases

    UniPathwayi UPA00908 ; UER00884 .
    BioCyci EcoCyc:PPPGPPHYDRO-MONOMER.
    ECOL316407:JW5603-MONOMER.
    MetaCyc:PPPGPPHYDRO-MONOMER.

    Miscellaneous databases

    PROi P25552.

    Gene expression databases

    Genevestigatori P25552.

    Family and domain databases

    HAMAPi MF_01550. GppA.
    InterProi IPR023709. Guo-5TP_3DP_PyrP.
    IPR003695. Ppx_GppA.
    [Graphical view ]
    Pfami PF02541. Ppx-GppA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "rhlB, a new Escherichia coli K-12 gene with an RNA helicase-like protein sequence motif, one of at least five such possible genes in a prokaryote."
      Kalman M., Murphy H., Cashel M.
      New Biol. 3:886-895(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
      Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
      Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: SEQUENCE REVISION TO 86 AND 423.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Exopolyphosphate phosphatase and guanosine pentaphosphate phosphatase belong to the sugar kinase/actin/hsp 70 superfamily."
      Reizer J., Reizer A., Saier M.H. Jr., Bork B., Sander C.
      Trends Biochem. Sci. 18:247-248(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: SIMILARITY TO PPX.
    7. "Guanosine pentaphosphate phosphohydrolase of Escherichia coli is a long-chain exopolyphosphatase."
      Keasling J.D., Bertsch L., Kornberg A.
      Proc. Natl. Acad. Sci. U.S.A. 90:7029-7033(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiGPPA_ECOLI
    AccessioniPrimary (citable) accession number: P25552
    Secondary accession number(s): Q2M887, Q6BF04
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 102 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3