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P25552

- GPPA_ECOLI

UniProt

P25552 - GPPA_ECOLI

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Protein
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
Gene
gppA, gpp, b3779, JW5603
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities.UniRule annotation

Catalytic activityi

Guanosine 5'-triphosphate,3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. guanosine-5'-triphosphate,3'-diphosphate diphosphatase activity Source: EcoCyc

GO - Biological processi

  1. guanosine pentaphosphate catabolic process Source: InterPro
  2. guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway
  3. nucleobase-containing small molecule interconversion Source: EcoCyc
  4. phosphorus metabolic process Source: EcoCyc
  5. response to starvation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:PPPGPPHYDRO-MONOMER.
ECOL316407:JW5603-MONOMER.
MetaCyc:PPPGPPHYDRO-MONOMER.
UniPathwayiUPA00908; UER00884.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (EC:3.6.1.40)
Alternative name(s):
Guanosine pentaphosphate phosphohydrolase
pppGpp-5'-phosphohydrolase
Gene namesi
Name:gppA
Synonyms:gpp
Ordered Locus Names:b3779, JW5603
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10413. gpp.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Guanosine-5'-triphosphate,3'-diphosphate pyrophosphataseUniRule annotation
PRO_0000194280Add
BLAST

Proteomic databases

PaxDbiP25552.
PRIDEiP25552.

Expressioni

Gene expression databases

GenevestigatoriP25552.

Interactioni

Protein-protein interaction databases

STRINGi511145.b3779.

Structurei

3D structure databases

ProteinModelPortaliP25552.
SMRiP25552. Positions 8-493.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0248.
HOGENOMiHOG000258672.
KOiK01524.
OMAiGLHIEYK.
OrthoDBiEOG6M6JN6.
PhylomeDBiP25552.

Family and domain databases

HAMAPiMF_01550. GppA.
InterProiIPR023709. Guo-5TP_3DP_PyrP.
IPR003695. Ppx_GppA.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25552-1 [UniParc]FASTAAdd to Basket

« Hide

MGSTSSLYAA IDLGSNSFHM LVVREVAGSI QTLTRIKRKV RLAAGLNSEN    50
ALSNEAMERG WQCLRLFAER LQDIPPSQIR VVATATLRLA VNAGDFIAKA 100
QEILGCPVQV ISGEEEARLI YQGVAHTTGG ADQRLVVDIG GASTELVTGT 150
GAQTTSLFSL SMGCVTWLER YFADRNLGQE NFDAAEKAAR EVLRPVADEL 200
RYHGWKVCVG ASGTVQALQE IMMAQGMDER ITLEKLQQLK QRAIHCGRLE 250
ELEIDGLTLE RALVFPSGLA ILIAIFTELN IQCMTLAGGA LREGLVYGML 300
HLAVEQDIRS RTLRNIQRRF MIDIDQAQRV AKVAANFFDQ VENEWHLEAI 350
SRDLLISACQ LHEIGLSVDF KQAPQHAAYL VRNLDLPGFT PAQKKLLATL 400
LLNQTNPVDL SSLHQQNAVP PRVAEQLCRL LRLAIIFASR RRDDLVPEMT 450
LQANHELLTL TLPQGWLTQH PLGKEIIAQE SQWQSYVHWP LEVH 494
Length:494
Mass (Da):54,871
Last modified:October 11, 2004 - v3
Checksum:iC4DD8DC59432A5F9
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti86 – 861T → R in AAA67580. 1 Publication
Sequence conflicti423 – 4231V → L in AAA67580. 1 Publication
Sequence conflicti437 – 49458FASRR…PLEVH → VGQPSP in AAB59049. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M83316 Genomic DNA. Translation: AAB59049.1.
M87049 Genomic DNA. Translation: AAA67580.1.
U00096 Genomic DNA. Translation: AAT48210.1.
AP009048 Genomic DNA. Translation: BAE77519.1.
PIRiA48285.
RefSeqiYP_026252.1. NC_000913.3.
YP_491660.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAT48210; AAT48210; b3779.
BAE77519; BAE77519; BAE77519.
GeneIDi12934325.
948291.
KEGGiecj:Y75_p3396.
eco:b3779.
PATRICi32123049. VBIEscCol129921_3894.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M83316 Genomic DNA. Translation: AAB59049.1 .
M87049 Genomic DNA. Translation: AAA67580.1 .
U00096 Genomic DNA. Translation: AAT48210.1 .
AP009048 Genomic DNA. Translation: BAE77519.1 .
PIRi A48285.
RefSeqi YP_026252.1. NC_000913.3.
YP_491660.1. NC_007779.1.

3D structure databases

ProteinModelPortali P25552.
SMRi P25552. Positions 8-493.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 511145.b3779.

Proteomic databases

PaxDbi P25552.
PRIDEi P25552.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT48210 ; AAT48210 ; b3779 .
BAE77519 ; BAE77519 ; BAE77519 .
GeneIDi 12934325.
948291.
KEGGi ecj:Y75_p3396.
eco:b3779.
PATRICi 32123049. VBIEscCol129921_3894.

Organism-specific databases

EchoBASEi EB0408.
EcoGenei EG10413. gpp.

Phylogenomic databases

eggNOGi COG0248.
HOGENOMi HOG000258672.
KOi K01524.
OMAi GLHIEYK.
OrthoDBi EOG6M6JN6.
PhylomeDBi P25552.

Enzyme and pathway databases

UniPathwayi UPA00908 ; UER00884 .
BioCyci EcoCyc:PPPGPPHYDRO-MONOMER.
ECOL316407:JW5603-MONOMER.
MetaCyc:PPPGPPHYDRO-MONOMER.

Miscellaneous databases

PROi P25552.

Gene expression databases

Genevestigatori P25552.

Family and domain databases

HAMAPi MF_01550. GppA.
InterProi IPR023709. Guo-5TP_3DP_PyrP.
IPR003695. Ppx_GppA.
[Graphical view ]
Pfami PF02541. Ppx-GppA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "rhlB, a new Escherichia coli K-12 gene with an RNA helicase-like protein sequence motif, one of at least five such possible genes in a prokaryote."
    Kalman M., Murphy H., Cashel M.
    New Biol. 3:886-895(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: SEQUENCE REVISION TO 86 AND 423.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Exopolyphosphate phosphatase and guanosine pentaphosphate phosphatase belong to the sugar kinase/actin/hsp 70 superfamily."
    Reizer J., Reizer A., Saier M.H. Jr., Bork B., Sander C.
    Trends Biochem. Sci. 18:247-248(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIMILARITY TO PPX.
  7. "Guanosine pentaphosphate phosphohydrolase of Escherichia coli is a long-chain exopolyphosphatase."
    Keasling J.D., Bertsch L., Kornberg A.
    Proc. Natl. Acad. Sci. U.S.A. 90:7029-7033(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiGPPA_ECOLI
AccessioniPrimary (citable) accession number: P25552
Secondary accession number(s): Q2M887, Q6BF04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 11, 2004
Last modified: May 14, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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