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P25552 (GPPA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase

EC=3.6.1.40
Alternative name(s):
Guanosine pentaphosphate phosphohydrolase
pppGpp-5'-phosphohydrolase
Gene names
Name:gppA
Synonyms:gpp
Ordered Locus Names:b3779, JW5603
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities. HAMAP-Rule MF_01550

Catalytic activity

Guanosine 5'-triphosphate,3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate. HAMAP-Rule MF_01550

Pathway

Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2. HAMAP-Rule MF_01550

Sequence similarities

Belongs to the GppA/Ppx family. GppA subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase HAMAP-Rule MF_01550
PRO_0000194280

Experimental info

Sequence conflict861T → R in AAA67580. Ref.2
Sequence conflict4231V → L in AAA67580. Ref.2
Sequence conflict437 – 49458FASRR…PLEVH → VGQPSP in AAB59049. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P25552 [UniParc].

Last modified October 11, 2004. Version 3.
Checksum: C4DD8DC59432A5F9

FASTA49454,871
        10         20         30         40         50         60 
MGSTSSLYAA IDLGSNSFHM LVVREVAGSI QTLTRIKRKV RLAAGLNSEN ALSNEAMERG 

        70         80         90        100        110        120 
WQCLRLFAER LQDIPPSQIR VVATATLRLA VNAGDFIAKA QEILGCPVQV ISGEEEARLI 

       130        140        150        160        170        180 
YQGVAHTTGG ADQRLVVDIG GASTELVTGT GAQTTSLFSL SMGCVTWLER YFADRNLGQE 

       190        200        210        220        230        240 
NFDAAEKAAR EVLRPVADEL RYHGWKVCVG ASGTVQALQE IMMAQGMDER ITLEKLQQLK 

       250        260        270        280        290        300 
QRAIHCGRLE ELEIDGLTLE RALVFPSGLA ILIAIFTELN IQCMTLAGGA LREGLVYGML 

       310        320        330        340        350        360 
HLAVEQDIRS RTLRNIQRRF MIDIDQAQRV AKVAANFFDQ VENEWHLEAI SRDLLISACQ 

       370        380        390        400        410        420 
LHEIGLSVDF KQAPQHAAYL VRNLDLPGFT PAQKKLLATL LLNQTNPVDL SSLHQQNAVP 

       430        440        450        460        470        480 
PRVAEQLCRL LRLAIIFASR RRDDLVPEMT LQANHELLTL TLPQGWLTQH PLGKEIIAQE 

       490 
SQWQSYVHWP LEVH 

« Hide

References

« Hide 'large scale' references
[1]"rhlB, a new Escherichia coli K-12 gene with an RNA helicase-like protein sequence motif, one of at least five such possible genes in a prokaryote."
Kalman M., Murphy H., Cashel M.
New Biol. 3:886-895(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005."
Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L.
Nucleic Acids Res. 34:1-9(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 86 AND 423.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Exopolyphosphate phosphatase and guanosine pentaphosphate phosphatase belong to the sugar kinase/actin/hsp 70 superfamily."
Reizer J., Reizer A., Saier M.H. Jr., Bork B., Sander C.
Trends Biochem. Sci. 18:247-248(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY TO PPX.
[7]"Guanosine pentaphosphate phosphohydrolase of Escherichia coli is a long-chain exopolyphosphatase."
Keasling J.D., Bertsch L., Kornberg A.
Proc. Natl. Acad. Sci. U.S.A. 90:7029-7033(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M83316 Genomic DNA. Translation: AAB59049.1.
M87049 Genomic DNA. Translation: AAA67580.1.
U00096 Genomic DNA. Translation: AAT48210.1.
AP009048 Genomic DNA. Translation: BAE77519.1.
PIRA48285.
RefSeqYP_026252.1. NC_000913.3.
YP_491660.1. NC_007779.1.

3D structure databases

ProteinModelPortalP25552.
SMRP25552. Positions 8-493.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING511145.b3779.

Proteomic databases

PaxDbP25552.
PRIDEP25552.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT48210; AAT48210; b3779.
BAE77519; BAE77519; BAE77519.
GeneID12934325.
948291.
KEGGecj:Y75_p3396.
eco:b3779.
PATRIC32123049. VBIEscCol129921_3894.

Organism-specific databases

EchoBASEEB0408.
EcoGeneEG10413. gpp.

Phylogenomic databases

eggNOGCOG0248.
HOGENOMHOG000258672.
KOK01524.
OMAGLHIEYK.
OrthoDBEOG6M6JN6.
PhylomeDBP25552.

Enzyme and pathway databases

BioCycEcoCyc:PPPGPPHYDRO-MONOMER.
ECOL316407:JW5603-MONOMER.
MetaCyc:PPPGPPHYDRO-MONOMER.
UniPathwayUPA00908; UER00884.

Gene expression databases

GenevestigatorP25552.

Family and domain databases

HAMAPMF_01550. GppA.
InterProIPR023709. Guo-5TP_3DP_PyrP.
IPR003695. Ppx_GppA.
[Graphical view]
PfamPF02541. Ppx-GppA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP25552.

Entry information

Entry nameGPPA_ECOLI
AccessionPrimary (citable) accession number: P25552
Secondary accession number(s): Q2M887, Q6BF04
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 11, 2004
Last modified: May 14, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene