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Protein

Anaerobic sulfatase-maturating enzyme homolog AslB

Gene

aslB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity
  • iron-sulfur clusterCuratedNote: May bind at least one other iron-sulfur cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi21 – 211Iron-sulfur 1 (4Fe-4S-S-AdoMet)Sequence analysis
Metal bindingi25 – 251Iron-sulfur 1 (4Fe-4S-S-AdoMet)Sequence analysis
Metal bindingi28 – 281Iron-sulfur 1 (4Fe-4S-S-AdoMet)Sequence analysis
Metal bindingi276 – 2761Iron-sulfurSequence analysis
Metal bindingi282 – 2821Iron-sulfurSequence analysis
Metal bindingi297 – 2971Iron-sulfurSequence analysis
Metal bindingi339 – 3391Iron-sulfurSequence analysis
Metal bindingi342 – 3421Iron-sulfurSequence analysis
Metal bindingi348 – 3481Iron-sulfurSequence analysis
Metal bindingi352 – 3521Iron-sulfurSequence analysis

GO - Molecular functioni

GO - Biological processi

  • protein maturation Source: EcoCyc
Complete GO annotation...

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10090-MONOMER.
ECOL316407:JW5594-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaerobic sulfatase-maturating enzyme homolog AslB
Short name:
AnSME homolog
Gene namesi
Name:aslB
Synonyms:atsB
Ordered Locus Names:b3800, JW5594
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10090. aslB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Anaerobic sulfatase-maturating enzyme homolog AslBPRO_0000134459Add
BLAST

Proteomic databases

PaxDbiP25550.
PRIDEiP25550.

Interactioni

Protein-protein interaction databases

BioGridi4259322. 5 interactions.
STRINGi511145.b3800.

Structurei

3D structure databases

ProteinModelPortaliP25550.
SMRiP25550. Positions 12-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C4X. Bacteria.
COG0641. LUCA.
HOGENOMiHOG000000300.
InParanoidiP25550.
KOiK06871.
OMAiEHNGDLY.
OrthoDBiEOG60PH7N.
PhylomeDBiP25550.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR023885. 4Fe4S-binding_SPASM_dom.
IPR013785. Aldolase_TIM.
IPR007197. rSAM.
IPR023867. Sulphatase_maturase_rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
PF13186. SPASM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR04085. rSAM_more_4Fe4S. 1 hit.
TIGR03942. sulfatase_rSAM. 1 hit.

Sequencei

Sequence statusi: Complete.

P25550-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQQVPTRAF HVMAKPSGSD CNLNCDYCFY LEKQSLYREK PVTHMDDDTL
60 70 80 90 100
EAYVRHYIAA SEPQNEVAFT WQGGEPTLLG LAFYRRAVAL QAKYGAGRKI
110 120 130 140 150
SNSFQTNGVL LDDEWCAFLA EHHFLVGLSL DGPPEIHNQY RVTKGGRPTH
160 170 180 190 200
KLVMRALTLL QKHHVDYNVL VCVNRTSAQQ PLQVYDFLCD AGVEFIQFIP
210 220 230 240 250
VVERLADETT ARDGLKLHAP GDIQGELTEW SVRPEEFGEF LVAIFDHWIK
260 270 280 290 300
RDVGKIFVMN IEWAFANFVG APGAVCHHQP TCGRSVIVEH NGDVYACDHY
310 320 330 340 350
VYPQYRLGNM HQQTIAEMID SPQQQAFGED KFKQLPAQCR SCNVLKACWG
360 370 380 390 400
GCPKHRFMLD ASGKPGLNYL CAGYQRYFRH LPPYLKAMAD LLAHGRPASD
410
IMHAHLLVVS K
Length:411
Mass (Da):46,513
Last modified:October 11, 2004 - v4
Checksum:iE819FE44BE9501AA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti116 – 1172CA → WP in AAA67596 (PubMed:1379743).Curated
Sequence conflicti391 – 3911L → C in AAA67596 (PubMed:1379743).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90498 Genomic DNA. Translation: AAC32035.1.
M87049 Genomic DNA. Translation: AAA67596.1.
U00096 Genomic DNA. Translation: AAT48217.1.
AP009048 Genomic DNA. Translation: BAE77501.1.
PIRiA65184.
RefSeqiWP_000941517.1. NZ_LN832404.1.
YP_026259.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48217; AAT48217; b3800.
BAE77501; BAE77501; BAE77501.
GeneIDi949013.
KEGGiecj:JW5594.
eco:b3800.
PATRICi32123097. VBIEscCol129921_3914.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90498 Genomic DNA. Translation: AAC32035.1.
M87049 Genomic DNA. Translation: AAA67596.1.
U00096 Genomic DNA. Translation: AAT48217.1.
AP009048 Genomic DNA. Translation: BAE77501.1.
PIRiA65184.
RefSeqiWP_000941517.1. NZ_LN832404.1.
YP_026259.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP25550.
SMRiP25550. Positions 12-390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259322. 5 interactions.
STRINGi511145.b3800.

Proteomic databases

PaxDbiP25550.
PRIDEiP25550.

Protocols and materials databases

DNASUi949013.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48217; AAT48217; b3800.
BAE77501; BAE77501; BAE77501.
GeneIDi949013.
KEGGiecj:JW5594.
eco:b3800.
PATRICi32123097. VBIEscCol129921_3914.

Organism-specific databases

EchoBASEiEB0088.
EcoGeneiEG10090. aslB.

Phylogenomic databases

eggNOGiENOG4105C4X. Bacteria.
COG0641. LUCA.
HOGENOMiHOG000000300.
InParanoidiP25550.
KOiK06871.
OMAiEHNGDLY.
OrthoDBiEOG60PH7N.
PhylomeDBiP25550.

Enzyme and pathway databases

BioCyciEcoCyc:EG10090-MONOMER.
ECOL316407:JW5594-MONOMER.

Miscellaneous databases

PROiP25550.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR023885. 4Fe4S-binding_SPASM_dom.
IPR013785. Aldolase_TIM.
IPR007197. rSAM.
IPR023867. Sulphatase_maturase_rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
PF13186. SPASM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR04085. rSAM_more_4Fe4S. 1 hit.
TIGR03942. sulfatase_rSAM. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Murphy H.R., Kalman M., Cashel M.
    Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 391.
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: SEQUENCE REVISION TO 116 AND 117.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Radical SAM, a novel protein superfamily linking unresolved steps in familiar biosynthetic pathways with radical mechanisms: functional characterization using new analysis and information visualization methods."
    Sofia H.J., Chen G., Hetzler B.G., Reyes-Spindola J.F., Miller N.E.
    Nucleic Acids Res. 29:1097-1106(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: MEMBER OF THE RADICAL SAM SUPERFAMILY.
  7. "First evidences for a third sulfatase maturation system in prokaryotes from E. coli aslB and ydeM deletion mutants."
    Benjdia A., Deho G., Rabot S., Berteau O.
    FEBS Lett. 581:1009-1014(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NON-INVOLVEMENT IN SULFATASE MATURATION.
    Strain: K12 / BW25113.

Entry informationi

Entry nameiASLB_ECOLI
AccessioniPrimary (citable) accession number: P25550
Secondary accession number(s): Q2M8A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 11, 2004
Last modified: January 20, 2016
This is version 113 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Despite its homology to the anaerobic sulfatase-maturating enzymes, it is not involved in Cys-type sulfatase maturation in vivo.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.