P25549 (ASLA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arylsulfatase
Short name=AS
EC=3.1.6.1

Alternative name(s):
Aryl-sulfate sulphohydrolase

Gene names

Name:	aslA
Synonyms:	atsA
Ordered Locus Names:	b3801, JW3773

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A phenol sulfate + H2O = a phenol + sulfate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Periplasm Potential.
Post-translational modification	The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity By similarity.
Sequence similarities	Belongs to the sulfatase family.
Sequence caution	The sequence AAC32036.1 differs from that shown. Reason: Frameshift at position 462.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arylsulfatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 551	527	Arylsulfatase		PRO_0000033446
Sites
	Active site	190	1	Potential
	Metal binding	94	1	Calcium By similarity
	Metal binding	95	1	Calcium By similarity
	Metal binding	136	1	Calcium; via 3-oxoalanine By similarity
	Metal binding	356	1	Calcium By similarity
	Metal binding	357	1	Calcium By similarity
Amino acid modifications
	Modified residue	136	1	3-oxoalanine (Ser) By similarity
Experimental info
	Sequence conflict	191	1	M → I in AAC32036. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P25549 [UniParc]. Last modified August 1, 1992. Version 2. Checksum: A04C8BBE5E63E988	FASTA	551	60,718
	10 20 30 40 50 60 MEFSFSPKRL VVAVAAALPL MASAADTPST ATARKGFAGY DHPNQYLVKP ATTIADNMMP 70 80 90 100 110 120 VMQHPAQDKE TQQKLAELEK KTGKKPNVVV FLLDDVGWMD VGFNGGGVAV GNPTPDIDAV 130 140 150 160 170 180 ASQGLILTSA YSQPSSSPTR ATILTGQYSI HHGILMPPMY GQPGGLQGLT TLPQLLHDQG 190 200 210 220 230 240 YVTQAIGKWH MGENKESQPQ NVGFDDFRGF NSVSDMYTEW RDVHVNPEVA LSPDRSEYIK 250 260 270 280 290 300 QLPFSKDDVH AVRGGEQQAI ADITPKYMED LDQRWMDYGV KFLDKMAKSD KPFFLYYGTR 310 320 330 340 350 360 GCHFDNYPNA KYAGSSPART SYGDCMVEMN DVFANLYKTL EKNGQLDNTL IVFTSDNGPE 370 380 390 400 410 420 AEVPPHGRTP FRGAKGSTWE GGVRVPTFVY WKGMIQPRKS DGIVDLADLF PTALDLAGHP 430 440 450 460 470 480 GAKVANLVPK TTFIDGVDQT SFFLGTNGQS NRKAEHYFLN GKLAAVRMDE FKYHVLIQQP 490 500 510 520 530 540 YAYTQSGYQG GFTGTVMQTA GSSVFNLYTD PQESDSIGVR HIPMGVPLQT EMHAYMEILK 550 KYPPRAQIKS D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the gppB locus as two convergent arylsulfatase-like genes, aslA and aslB, capable of suppressing a guanosine pentaphosphate phosphatase missense mutation in Escherichia coli." Murphy H.R., Kalman M., Cashel M. Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R. Science 257:771-778(1992) [PubMed: 1379743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M90498 Genomic DNA. Translation: AAC32036.1. Frameshift. M87049 Genomic DNA. Translation: AAA67597.1. U00096 Genomic DNA. Translation: AAC76804.1. AP009048 Genomic DNA. Translation: BAE77500.1.
PIR	S30691.
RefSeq	NP_418245.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P25549.
SMR	P25549. Positions 84-541.
ModBase	Search...
Protein-protein interaction databases
IntAct	P25549. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000559; EBESCP00000000559; EBESCG00000000468. EBESCT00000017339; EBESCP00000016630; EBESCG00000016395.
GeneID	949015.
GenomeReviews	Gene locus JW3773 in contig AP009048_GR. Gene locus b3801 in contig U00096_GR.
KEGG	ecj:JW3773. eco:b3801.
PATRIC	32123099. VBIEscCol129921_3915.
Organism-specific databases
EchoBASE	EB0087.
EcoGene	EG10089. aslA.
Phylogenomic databases
eggNOG	COG3119.
GeneTree	EBGT00050000009139.
HOGENOM	HBG374065.
OMA	PTRATIM.
PhylomeDB	P25549.
ProtClustDB	CLSK880785.
Enzyme and pathway databases
BioCyc	EcoCyc:ARYLSULFAT-MONOMER. MetaCyc:ARYLSULFAT-MONOMER.
Gene expression databases
Genevestigator	P25549.
Family and domain databases
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR017850. Alkaline_phosphatase_core. IPR000917. Sulfatase. IPR024607. Sulfatase_CS. [Graphical view]
Gene3D	G3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
KO	K01130.
Pfam	PF00884. Sulfatase. 1 hit. [Graphical view]
SUPFAM	SSF53649. Alkaline_phosphatase_core. 1 hit.
PROSITE	PS00523. SULFATASE_1. 1 hit. PS00149. SULFATASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASLA_ECOLI

Accession

Primary (citable) accession number: P25549
Secondary accession number(s): Q2M8A6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 25, 2012
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program