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Protein

Arylsulfatase

Gene

aslA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

A phenol sulfate + H2O = a phenol + sulfate.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi94 – 941CalciumBy similarity
Metal bindingi95 – 951CalciumBy similarity
Metal bindingi136 – 1361Calcium; via 3-oxoalanineBy similarity
Active sitei190 – 1901Sequence analysis
Metal bindingi356 – 3561CalciumBy similarity
Metal bindingi357 – 3571CalciumBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:ARYLSULFAT-MONOMER.
ECOL316407:JW3773-MONOMER.
MetaCyc:ARYLSULFAT-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Arylsulfatase (EC:3.1.6.1)
Short name:
AS
Alternative name(s):
Aryl-sulfate sulphohydrolase
Gene namesi
Name:aslA
Synonyms:atsA
Ordered Locus Names:b3801, JW3773
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10089. aslA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 551527ArylsulfatasePRO_0000033446Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei136 – 13613-oxoalanine (Ser)By similarity

Post-translational modificationi

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity

Proteomic databases

PaxDbiP25549.

Interactioni

Protein-protein interaction databases

BioGridi4262605. 5 interactions.
IntActiP25549. 1 interaction.
STRINGi511145.b3801.

Structurei

3D structure databases

ProteinModelPortaliP25549.
SMRiP25549. Positions 84-547.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105ENH. Bacteria.
COG3119. LUCA.
HOGENOMiHOG000126460.
InParanoidiP25549.
KOiK01130.
OMAiETHILTP.
OrthoDBiEOG6SBSZ8.
PhylomeDBiP25549.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25549-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFSFSPKRL VVAVAAALPL MASAADTPST ATARKGFAGY DHPNQYLVKP
60 70 80 90 100
ATTIADNMMP VMQHPAQDKE TQQKLAELEK KTGKKPNVVV FLLDDVGWMD
110 120 130 140 150
VGFNGGGVAV GNPTPDIDAV ASQGLILTSA YSQPSSSPTR ATILTGQYSI
160 170 180 190 200
HHGILMPPMY GQPGGLQGLT TLPQLLHDQG YVTQAIGKWH MGENKESQPQ
210 220 230 240 250
NVGFDDFRGF NSVSDMYTEW RDVHVNPEVA LSPDRSEYIK QLPFSKDDVH
260 270 280 290 300
AVRGGEQQAI ADITPKYMED LDQRWMDYGV KFLDKMAKSD KPFFLYYGTR
310 320 330 340 350
GCHFDNYPNA KYAGSSPART SYGDCMVEMN DVFANLYKTL EKNGQLDNTL
360 370 380 390 400
IVFTSDNGPE AEVPPHGRTP FRGAKGSTWE GGVRVPTFVY WKGMIQPRKS
410 420 430 440 450
DGIVDLADLF PTALDLAGHP GAKVANLVPK TTFIDGVDQT SFFLGTNGQS
460 470 480 490 500
NRKAEHYFLN GKLAAVRMDE FKYHVLIQQP YAYTQSGYQG GFTGTVMQTA
510 520 530 540 550
GSSVFNLYTD PQESDSIGVR HIPMGVPLQT EMHAYMEILK KYPPRAQIKS

D
Length:551
Mass (Da):60,718
Last modified:August 1, 1992 - v2
Checksum:iA04C8BBE5E63E988
GO

Sequence cautioni

The sequence AAC32036.1 differs from that shown. Reason: Frameshift at position 462. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti191 – 1911M → I in AAC32036 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90498 Genomic DNA. Translation: AAC32036.1. Frameshift.
M87049 Genomic DNA. Translation: AAA67597.1.
U00096 Genomic DNA. Translation: AAC76804.1.
AP009048 Genomic DNA. Translation: BAE77500.1.
PIRiS30691.
RefSeqiNP_418245.1. NC_000913.3.
WP_000395863.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76804; AAC76804; b3801.
BAE77500; BAE77500; BAE77500.
GeneIDi949015.
KEGGiecj:JW3773.
eco:b3801.
PATRICi32123099. VBIEscCol129921_3915.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90498 Genomic DNA. Translation: AAC32036.1. Frameshift.
M87049 Genomic DNA. Translation: AAA67597.1.
U00096 Genomic DNA. Translation: AAC76804.1.
AP009048 Genomic DNA. Translation: BAE77500.1.
PIRiS30691.
RefSeqiNP_418245.1. NC_000913.3.
WP_000395863.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP25549.
SMRiP25549. Positions 84-547.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262605. 5 interactions.
IntActiP25549. 1 interaction.
STRINGi511145.b3801.

Proteomic databases

PaxDbiP25549.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76804; AAC76804; b3801.
BAE77500; BAE77500; BAE77500.
GeneIDi949015.
KEGGiecj:JW3773.
eco:b3801.
PATRICi32123099. VBIEscCol129921_3915.

Organism-specific databases

EchoBASEiEB0087.
EcoGeneiEG10089. aslA.

Phylogenomic databases

eggNOGiENOG4105ENH. Bacteria.
COG3119. LUCA.
HOGENOMiHOG000126460.
InParanoidiP25549.
KOiK01130.
OMAiETHILTP.
OrthoDBiEOG6SBSZ8.
PhylomeDBiP25549.

Enzyme and pathway databases

BioCyciEcoCyc:ARYLSULFAT-MONOMER.
ECOL316407:JW3773-MONOMER.
MetaCyc:ARYLSULFAT-MONOMER.

Miscellaneous databases

PROiP25549.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the gppB locus as two convergent arylsulfatase-like genes, aslA and aslB, capable of suppressing a guanosine pentaphosphate phosphatase missense mutation in Escherichia coli."
    Murphy H.R., Kalman M., Cashel M.
    Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiASLA_ECOLI
AccessioniPrimary (citable) accession number: P25549
Secondary accession number(s): Q2M8A6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: August 1, 1992
Last modified: January 20, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.