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Protein

2-octaprenyl-6-methoxyphenol hydroxylase

Gene

ubiH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Is likely an oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinol.1 Publication

Cofactori

FADBy similarity

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • response to light stimulus Source: EcoliWiki
  • response to oxidative stress Source: EcoliWiki
  • secondary metabolite biosynthetic process Source: GO_Central
  • ubiquinone biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Ubiquinone biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:OCTAPRENYL-METHOXYPHENOL-OH-MONOMER.
ECOL316407:JW2875-MONOMER.
MetaCyc:OCTAPRENYL-METHOXYPHENOL-OH-MONOMER.
BRENDAi1.14.99.B5. 2026.
UniPathwayiUPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
2-octaprenyl-6-methoxyphenol hydroxylase (EC:1.14.13.-)
Gene namesi
Name:ubiH
Synonyms:visB
Ordered Locus Names:b2907, JW2875
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11324. ubiH.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are photosensitive and are not able to grow with a nonfermentable source of carbon. They also fail to produce ubiquinone, and accumulate 2-octaprenylphenol and 2-octaprenyl-6-methoxyphenol.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3923922-octaprenyl-6-methoxyphenol hydroxylasePRO_0000207579Add
BLAST

Proteomic databases

PaxDbiP25534.

Interactioni

Protein-protein interaction databases

BioGridi4262345. 372 interactions.
DIPiDIP-11071N.
IntActiP25534. 13 interactions.
MINTiMINT-1289084.
STRINGi511145.b2907.

Structurei

3D structure databases

ProteinModelPortaliP25534.
SMRiP25534. Positions 1-337.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UbiH/COQ6 family.Curated

Phylogenomic databases

eggNOGiENOG4105D0A. Bacteria.
COG0654. LUCA.
HOGENOMiHOG000255770.
InParanoidiP25534.
KOiK03185.
OMAiGIVNQTP.
OrthoDBiEOG6G20HJ.
PhylomeDBiP25534.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
IPR018168. Ubi_Hdrlase_CS.
IPR011295. UbiH.
IPR010971. UbiH/COQ6.
[Graphical view]
PfamiPF01494. FAD_binding_3. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR01988. Ubi-OHases. 1 hit.
TIGR01984. UbiH. 1 hit.
PROSITEiPS01304. UBIH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVIIVGGGM AGATLALAIS RLSHGALPVH LIEATAPESH AHPGFDGRAI
60 70 80 90 100
ALAAGTCQQL ARIGVWQSLA DCATAITTVH VSDRGHAGFV TLAAEDYQLA
110 120 130 140 150
ALGQVVELHN VGQRLFALLR KAPGVTLHCP DRVANVARTQ SHVEVTLESG
160 170 180 190 200
ETLTGRVLVA ADGTHSALAT ACGVDWQQEP YEQLAVIANV ATSVAHEGRA
210 220 230 240 250
FERFTQHGPL AMLPMSDGRC SLVWCHPLER REEVLSWSDE KFCRELQSAF
260 270 280 290 300
GWRLGKITHA GKRSAYPLAL THAARSITHR TVLVGNAAQT LHPIAGQGFN
310 320 330 340 350
LGMRDVMSLA ETLTQAQERG EDMGDYGVLC RYQQRRQSDR EATIGVTDSL
360 370 380 390
VHLFANRWAP LVVGRNIGLM TMELFTPARD VLAQRTLGWV AR
Length:392
Mass (Da):42,288
Last modified:August 29, 2003 - v2
Checksum:i3CCE10DAA00A7FC5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti382 – 3821L → V in BAA14326 (PubMed:1339425).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90281 Genomic DNA. Translation: BAA14326.1.
U28377 Genomic DNA. Translation: AAA69075.1.
U00096 Genomic DNA. Translation: AAC75945.1.
AP009048 Genomic DNA. Translation: BAE76972.1.
PIRiC65075.
RefSeqiNP_417383.1. NC_000913.3.
WP_000111195.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75945; AAC75945; b2907.
BAE76972; BAE76972; BAE76972.
GeneIDi947388.
KEGGiecj:JW2875.
eco:b2907.
PATRICi32121228. VBIEscCol129921_3002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90281 Genomic DNA. Translation: BAA14326.1.
U28377 Genomic DNA. Translation: AAA69075.1.
U00096 Genomic DNA. Translation: AAC75945.1.
AP009048 Genomic DNA. Translation: BAE76972.1.
PIRiC65075.
RefSeqiNP_417383.1. NC_000913.3.
WP_000111195.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP25534.
SMRiP25534. Positions 1-337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262345. 372 interactions.
DIPiDIP-11071N.
IntActiP25534. 13 interactions.
MINTiMINT-1289084.
STRINGi511145.b2907.

Proteomic databases

PaxDbiP25534.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75945; AAC75945; b2907.
BAE76972; BAE76972; BAE76972.
GeneIDi947388.
KEGGiecj:JW2875.
eco:b2907.
PATRICi32121228. VBIEscCol129921_3002.

Organism-specific databases

EchoBASEiEB1300.
EcoGeneiEG11324. ubiH.

Phylogenomic databases

eggNOGiENOG4105D0A. Bacteria.
COG0654. LUCA.
HOGENOMiHOG000255770.
InParanoidiP25534.
KOiK03185.
OMAiGIVNQTP.
OrthoDBiEOG6G20HJ.
PhylomeDBiP25534.

Enzyme and pathway databases

UniPathwayiUPA00232.
BioCyciEcoCyc:OCTAPRENYL-METHOXYPHENOL-OH-MONOMER.
ECOL316407:JW2875-MONOMER.
MetaCyc:OCTAPRENYL-METHOXYPHENOL-OH-MONOMER.
BRENDAi1.14.99.B5. 2026.

Miscellaneous databases

PROiP25534.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
IPR018168. Ubi_Hdrlase_CS.
IPR011295. UbiH.
IPR010971. UbiH/COQ6.
[Graphical view]
PfamiPF01494. FAD_binding_3. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR01988. Ubi-OHases. 1 hit.
TIGR01984. UbiH. 1 hit.
PROSITEiPS01304. UBIH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a light-sensitive mutant of Escherichia coli K-12 with a mutation in a gene that is required for the biosynthesis of ubiquinone."
    Nakahigashi K., Miyamoto K., Nishimura K., Inokuchi H.
    J. Bacteriol. 174:7352-7359(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Pathway for ubiquinone biosynthesis in Escherichia coli K-12: gene-enzyme relationships and intermediates."
    Young I.G., Stroobant P., Macdonald C.G., Gibson F.
    J. Bacteriol. 114:42-52(1973) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN UBIQUINONE BIOSYNTHESIS, DISRUPTION PHENOTYPE.
    Strain: K12.

Entry informationi

Entry nameiUBIH_ECOLI
AccessioniPrimary (citable) accession number: P25534
Secondary accession number(s): Q2M9T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: August 29, 2003
Last modified: January 20, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.