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Protein

2-octaprenyl-6-methoxyphenol hydroxylase

Gene

ubiH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Is likely an oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinol.1 Publication

Cofactori

FADBy similarity

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • response to light stimulus Source: EcoliWiki
  • response to oxidative stress Source: EcoliWiki
  • ubiquinone biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processUbiquinone biosynthesis
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:OCTAPRENYL-METHOXYPHENOL-OH-MONOMER
MetaCyc:OCTAPRENYL-METHOXYPHENOL-OH-MONOMER
BRENDAi1.14.99.B5 2026
UniPathwayiUPA00232

Names & Taxonomyi

Protein namesi
Recommended name:
2-octaprenyl-6-methoxyphenol hydroxylase (EC:1.14.13.-)
Gene namesi
Name:ubiH
Synonyms:visB
Ordered Locus Names:b2907, JW2875
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11324 ubiH

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are photosensitive and are not able to grow with a nonfermentable source of carbon. They also fail to produce ubiquinone, and accumulate 2-octaprenylphenol and 2-octaprenyl-6-methoxyphenol.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002075791 – 3922-octaprenyl-6-methoxyphenol hydroxylaseAdd BLAST392

Proteomic databases

PaxDbiP25534
PRIDEiP25534

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
hldDP679102EBI-559377,EBI-543760

Protein-protein interaction databases

BioGridi4262345, 372 interactors
DIPiDIP-11071N
IntActiP25534, 13 interactors
STRINGi316385.ECDH10B_3081

Structurei

3D structure databases

ProteinModelPortaliP25534
SMRiP25534
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UbiH/COQ6 family.Curated

Phylogenomic databases

eggNOGiENOG4105D0A Bacteria
COG0654 LUCA
HOGENOMiHOG000255770
InParanoidiP25534
KOiK03185
OMAiGNAAHSL
PhylomeDBiP25534

Family and domain databases

Gene3Di3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR002938 FAD-bd
IPR036188 FAD/NAD-bd_sf
IPR018168 Ubi_Hdrlase_CS
IPR011295 UbiH
IPR010971 UbiH/COQ6
PfamiView protein in Pfam
PF01494 FAD_binding_3, 1 hit
SUPFAMiSSF51905 SSF51905, 2 hits
TIGRFAMsiTIGR01988 Ubi-OHases, 1 hit
TIGR01984 UbiH, 1 hit
PROSITEiView protein in PROSITE
PS01304 UBIH, 1 hit

Sequencei

Sequence statusi: Complete.

P25534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVIIVGGGM AGATLALAIS RLSHGALPVH LIEATAPESH AHPGFDGRAI
60 70 80 90 100
ALAAGTCQQL ARIGVWQSLA DCATAITTVH VSDRGHAGFV TLAAEDYQLA
110 120 130 140 150
ALGQVVELHN VGQRLFALLR KAPGVTLHCP DRVANVARTQ SHVEVTLESG
160 170 180 190 200
ETLTGRVLVA ADGTHSALAT ACGVDWQQEP YEQLAVIANV ATSVAHEGRA
210 220 230 240 250
FERFTQHGPL AMLPMSDGRC SLVWCHPLER REEVLSWSDE KFCRELQSAF
260 270 280 290 300
GWRLGKITHA GKRSAYPLAL THAARSITHR TVLVGNAAQT LHPIAGQGFN
310 320 330 340 350
LGMRDVMSLA ETLTQAQERG EDMGDYGVLC RYQQRRQSDR EATIGVTDSL
360 370 380 390
VHLFANRWAP LVVGRNIGLM TMELFTPARD VLAQRTLGWV AR
Length:392
Mass (Da):42,288
Last modified:August 29, 2003 - v2
Checksum:i3CCE10DAA00A7FC5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti382L → V in BAA14326 (PubMed:1339425).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90281 Genomic DNA Translation: BAA14326.1
U28377 Genomic DNA Translation: AAA69075.1
U00096 Genomic DNA Translation: AAC75945.1
AP009048 Genomic DNA Translation: BAE76972.1
PIRiC65075
RefSeqiNP_417383.1, NC_000913.3
WP_000111195.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75945; AAC75945; b2907
BAE76972; BAE76972; BAE76972
GeneIDi947388
KEGGiecj:JW2875
eco:b2907
PATRICifig|1411691.4.peg.3825

Similar proteinsi

Entry informationi

Entry nameiUBIH_ECOLI
AccessioniPrimary (citable) accession number: P25534
Secondary accession number(s): Q2M9T4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: August 29, 2003
Last modified: March 28, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health