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P25524

- CODA_ECOLI

UniProt

P25524 - CODA_ECOLI

Protein

Cytosine deaminase

Gene

codA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Cytosine + H2O = uracil + NH3.

    Cofactori

    Iron or another divalent metal ion.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi62 – 621Iron
    Metal bindingi64 – 641Iron

    GO - Molecular functioni

    1. cytosine deaminase activity Source: EcoCyc
    2. ferrous iron binding Source: EcoCyc
    3. identical protein binding Source: IntAct
    4. isoguanine deaminase activity Source: EcoCyc
    5. zinc ion binding Source: EcoCyc

    GO - Biological processi

    1. cytosine catabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cytosine metabolism

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:CYTDEAM-MONOMER.
    ECOL316407:JW0328-MONOMER.
    MetaCyc:CYTDEAM-MONOMER.
    RETL1328306-WGS:GSTH-6950-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosine deaminase (EC:3.5.4.1)
    Alternative name(s):
    Cytosine aminohydrolase
    Gene namesi
    Name:codA
    Ordered Locus Names:b0337, JW0328
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11326. codA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 427426Cytosine deaminasePRO_0000090002Add
    BLAST

    Proteomic databases

    PaxDbiP25524.
    PRIDEiP25524.

    Expressioni

    Gene expression databases

    GenevestigatoriP25524.

    Interactioni

    Subunit structurei

    Homotetramer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-559181,EBI-559181

    Protein-protein interaction databases

    DIPiDIP-9306N.
    IntActiP25524. 6 interactions.
    MINTiMINT-1307853.
    STRINGi511145.b0337.

    Structurei

    Secondary structure

    1
    427
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 136
    Beta strandi19 – 268
    Beta strandi29 – 3810
    Beta strandi46 – 483
    Beta strandi53 – 564
    Beta strandi58 – 636
    Turni65 – 695
    Beta strandi73 – 753
    Helixi82 – 9312
    Helixi98 – 11417
    Beta strandi117 – 1259
    Helixi132 – 14413
    Turni145 – 1473
    Beta strandi149 – 1557
    Beta strandi160 – 1634
    Helixi166 – 17510
    Beta strandi179 – 1813
    Helixi185 – 1873
    Beta strandi188 – 1903
    Helixi191 – 20818
    Beta strandi211 – 2166
    Helixi226 – 23712
    Helixi240 – 2423
    Beta strandi243 – 2475
    Helixi249 – 2535
    Helixi256 – 26914
    Beta strandi272 – 2754
    Helixi277 – 2837
    Turni284 – 2874
    Helixi299 – 3046
    Beta strandi309 – 3113
    Beta strandi316 – 3183
    Helixi328 – 33811
    Helixi344 – 3485
    Helixi349 – 3546
    Helixi356 – 3616
    Beta strandi368 – 3703
    Beta strandi373 – 3753
    Beta strandi377 – 3848
    Helixi385 – 3917
    Beta strandi396 – 4005
    Beta strandi403 – 4075
    Beta strandi413 – 42311

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K6WX-ray1.75A2-427[»]
    1K70X-ray1.80A2-427[»]
    1R9XX-ray1.58A2-427[»]
    1R9YX-ray1.57A2-427[»]
    1R9ZX-ray1.32A2-427[»]
    1RA0X-ray1.12A2-427[»]
    1RA5X-ray1.40A2-427[»]
    1RAKX-ray1.32A2-427[»]
    3G77X-ray1.80A5-427[»]
    3O7UX-ray1.71A1-427[»]
    3R0DX-ray1.50A1-427[»]
    3RN6X-ray2.26A1-427[»]
    ProteinModelPortaliP25524.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP25524.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG0402.
    HOGENOMiHOG000184778.
    KOiK01485.
    OMAiNNINVCF.
    OrthoDBiEOG6QG8F9.
    PhylomeDBiP25524.

    Family and domain databases

    Gene3Di2.30.40.10. 1 hit.
    InterProiIPR013108. Amidohydro_3.
    IPR011059. Metal-dep_hydrolase_composite.
    [Graphical view]
    PfamiPF07969. Amidohydro_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF51338. SSF51338. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25524-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNNALQTII NARLPGEEGL WQIHLQDGKI SAIDAQSGVM PITENSLDAE    50
    QGLVIPPFVE PHIHLDTTQT AGQPNWNQSG TLFEGIERWA ERKALLTHDD 100
    VKQRAWQTLK WQIANGIQHV RTHVDVSDAT LTALKAMLEV KQEVAPWIDL 150
    QIVAFPQEGI LSYPNGEALL EEALRLGADV VGAIPHFEFT REYGVESLHK 200
    TFALAQKYDR LIDVHCDEID DEQSRFVETV AALAHHEGMG ARVTASHTTA 250
    MHSYNGAYTS RLFRLLKMSG INFVANPLVN IHLQGRFDTY PKRRGITRVK 300
    EMLESGINVC FGHDDVFDPW YPLGTANMLQ VLHMGLHVCQ LMGYGQINDG 350
    LNLITHHSAR TLNLQDYGIA AGNSANLIIL PAENGFDALR RQVPVRYSVR 400
    GGKVIASTQP AQTTVYLEQP EAIDYKR 427
    Length:427
    Mass (Da):47,591
    Last modified:January 23, 2007 - v3
    Checksum:i9F91A2C46B3B1E42
    GO

    Sequence cautioni

    The sequence AAB18061.1 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti13 – 131R → W in strain: SO5076.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63656 Genomic DNA. Translation: CAA45196.1.
    S56903 Genomic DNA. Translation: AAB25761.2.
    U73857 Genomic DNA. Translation: AAB18061.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC73440.1.
    AP009048 Genomic DNA. Translation: BAE76119.1.
    PIRiS22662.
    RefSeqiNP_414871.1. NC_000913.3.
    YP_488631.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73440; AAC73440; b0337.
    BAE76119; BAE76119; BAE76119.
    GeneIDi12930819.
    944996.
    KEGGiecj:Y75_p0326.
    eco:b0337.
    PATRICi32115805. VBIEscCol129921_0344.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63656 Genomic DNA. Translation: CAA45196.1 .
    S56903 Genomic DNA. Translation: AAB25761.2 .
    U73857 Genomic DNA. Translation: AAB18061.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC73440.1 .
    AP009048 Genomic DNA. Translation: BAE76119.1 .
    PIRi S22662.
    RefSeqi NP_414871.1. NC_000913.3.
    YP_488631.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K6W X-ray 1.75 A 2-427 [» ]
    1K70 X-ray 1.80 A 2-427 [» ]
    1R9X X-ray 1.58 A 2-427 [» ]
    1R9Y X-ray 1.57 A 2-427 [» ]
    1R9Z X-ray 1.32 A 2-427 [» ]
    1RA0 X-ray 1.12 A 2-427 [» ]
    1RA5 X-ray 1.40 A 2-427 [» ]
    1RAK X-ray 1.32 A 2-427 [» ]
    3G77 X-ray 1.80 A 5-427 [» ]
    3O7U X-ray 1.71 A 1-427 [» ]
    3R0D X-ray 1.50 A 1-427 [» ]
    3RN6 X-ray 2.26 A 1-427 [» ]
    ProteinModelPortali P25524.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9306N.
    IntActi P25524. 6 interactions.
    MINTi MINT-1307853.
    STRINGi 511145.b0337.

    Proteomic databases

    PaxDbi P25524.
    PRIDEi P25524.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73440 ; AAC73440 ; b0337 .
    BAE76119 ; BAE76119 ; BAE76119 .
    GeneIDi 12930819.
    944996.
    KEGGi ecj:Y75_p0326.
    eco:b0337.
    PATRICi 32115805. VBIEscCol129921_0344.

    Organism-specific databases

    EchoBASEi EB1302.
    EcoGenei EG11326. codA.

    Phylogenomic databases

    eggNOGi COG0402.
    HOGENOMi HOG000184778.
    KOi K01485.
    OMAi NNINVCF.
    OrthoDBi EOG6QG8F9.
    PhylomeDBi P25524.

    Enzyme and pathway databases

    BioCyci EcoCyc:CYTDEAM-MONOMER.
    ECOL316407:JW0328-MONOMER.
    MetaCyc:CYTDEAM-MONOMER.
    RETL1328306-WGS:GSTH-6950-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P25524.
    PROi P25524.

    Gene expression databases

    Genevestigatori P25524.

    Family and domain databases

    Gene3Di 2.30.40.10. 1 hit.
    InterProi IPR013108. Amidohydro_3.
    IPR011059. Metal-dep_hydrolase_composite.
    [Graphical view ]
    Pfami PF07969. Amidohydro_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51338. SSF51338. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the Escherichia coli codBA operon encoding cytosine permease and cytosine deaminase."
      Danielsen S., Kilstrup M., Barilla K., Jochimsen B., Neuhard J.
      Mol. Microbiol. 6:1335-1344(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20.
      Strain: CSH01.
    2. "A first step in the development of gene therapy for colorectal carcinoma: cloning, sequencing, and expression of Escherichia coli cytosine deaminase."
      Austin E.A., Huber B.E.
      Mol. Pharmacol. 43:380-387(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "The structure of Escherichia coli cytosine deaminase."
      Ireton G.C., McDermott G., Black M.E., Stoddard B.L.
      J. Mol. Biol. 315:687-697(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).

    Entry informationi

    Entry nameiCODA_ECOLI
    AccessioniPrimary (citable) accession number: P25524
    Secondary accession number(s): Q2MC87
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 123 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3