Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P25524

- CODA_ECOLI

UniProt

P25524 - CODA_ECOLI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Cytosine deaminase

Gene

codA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Cytosine + H2O = uracil + NH3.

Cofactori

Iron or another divalent metal ion.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Iron
Metal bindingi64 – 641Iron

GO - Molecular functioni

  1. cytosine deaminase activity Source: EcoCyc
  2. ferrous iron binding Source: EcoCyc
  3. identical protein binding Source: IntAct
  4. isoguanine deaminase activity Source: EcoCyc
  5. zinc ion binding Source: EcoCyc

GO - Biological processi

  1. cytosine catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cytosine metabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:CYTDEAM-MONOMER.
ECOL316407:JW0328-MONOMER.
MetaCyc:CYTDEAM-MONOMER.
RETL1328306-WGS:GSTH-6950-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosine deaminase (EC:3.5.4.1)
Alternative name(s):
Cytosine aminohydrolase
Gene namesi
Name:codA
Ordered Locus Names:b0337, JW0328
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11326. codA.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 427426Cytosine deaminasePRO_0000090002Add
BLAST

Proteomic databases

PaxDbiP25524.
PRIDEiP25524.

Expressioni

Gene expression databases

GenevestigatoriP25524.

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-559181,EBI-559181

Protein-protein interaction databases

DIPiDIP-9306N.
IntActiP25524. 6 interactions.
MINTiMINT-1307853.
STRINGi511145.b0337.

Structurei

Secondary structure

1
427
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 136
Beta strandi19 – 268
Beta strandi29 – 3810
Beta strandi46 – 483
Beta strandi53 – 564
Beta strandi58 – 636
Turni65 – 695
Beta strandi73 – 753
Helixi82 – 9312
Helixi98 – 11417
Beta strandi117 – 1259
Helixi132 – 14413
Turni145 – 1473
Beta strandi149 – 1557
Beta strandi160 – 1634
Helixi166 – 17510
Beta strandi179 – 1813
Helixi185 – 1873
Beta strandi188 – 1903
Helixi191 – 20818
Beta strandi211 – 2166
Helixi226 – 23712
Helixi240 – 2423
Beta strandi243 – 2475
Helixi249 – 2535
Helixi256 – 26914
Beta strandi272 – 2754
Helixi277 – 2837
Turni284 – 2874
Helixi299 – 3046
Beta strandi309 – 3113
Beta strandi316 – 3183
Helixi328 – 33811
Helixi344 – 3485
Helixi349 – 3546
Helixi356 – 3616
Beta strandi368 – 3703
Beta strandi373 – 3753
Beta strandi377 – 3848
Helixi385 – 3917
Beta strandi396 – 4005
Beta strandi403 – 4075
Beta strandi413 – 42311

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K6WX-ray1.75A2-427[»]
1K70X-ray1.80A2-427[»]
1R9XX-ray1.58A2-427[»]
1R9YX-ray1.57A2-427[»]
1R9ZX-ray1.32A2-427[»]
1RA0X-ray1.12A2-427[»]
1RA5X-ray1.40A2-427[»]
1RAKX-ray1.32A2-427[»]
3G77X-ray1.80A5-427[»]
3O7UX-ray1.71A1-427[»]
3R0DX-ray1.50A1-427[»]
3RN6X-ray2.26A1-427[»]
ProteinModelPortaliP25524.
SMRiP25524. Positions 5-427.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25524.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG0402.
HOGENOMiHOG000184778.
InParanoidiP25524.
KOiK01485.
OMAiNNINVCF.
OrthoDBiEOG6QG8F9.
PhylomeDBiP25524.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
InterProiIPR013108. Amidohydro_3.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PfamiPF07969. Amidohydro_3. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25524-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNNALQTII NARLPGEEGL WQIHLQDGKI SAIDAQSGVM PITENSLDAE
60 70 80 90 100
QGLVIPPFVE PHIHLDTTQT AGQPNWNQSG TLFEGIERWA ERKALLTHDD
110 120 130 140 150
VKQRAWQTLK WQIANGIQHV RTHVDVSDAT LTALKAMLEV KQEVAPWIDL
160 170 180 190 200
QIVAFPQEGI LSYPNGEALL EEALRLGADV VGAIPHFEFT REYGVESLHK
210 220 230 240 250
TFALAQKYDR LIDVHCDEID DEQSRFVETV AALAHHEGMG ARVTASHTTA
260 270 280 290 300
MHSYNGAYTS RLFRLLKMSG INFVANPLVN IHLQGRFDTY PKRRGITRVK
310 320 330 340 350
EMLESGINVC FGHDDVFDPW YPLGTANMLQ VLHMGLHVCQ LMGYGQINDG
360 370 380 390 400
LNLITHHSAR TLNLQDYGIA AGNSANLIIL PAENGFDALR RQVPVRYSVR
410 420
GGKVIASTQP AQTTVYLEQP EAIDYKR
Length:427
Mass (Da):47,591
Last modified:January 23, 2007 - v3
Checksum:i9F91A2C46B3B1E42
GO

Sequence cautioni

The sequence AAB18061.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti13 – 131R → W in strain: SO5076.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63656 Genomic DNA. Translation: CAA45196.1.
S56903 Genomic DNA. Translation: AAB25761.2.
U73857 Genomic DNA. Translation: AAB18061.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73440.1.
AP009048 Genomic DNA. Translation: BAE76119.1.
PIRiS22662.
RefSeqiNP_414871.1. NC_000913.3.
YP_488631.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73440; AAC73440; b0337.
BAE76119; BAE76119; BAE76119.
GeneIDi12930819.
944996.
KEGGiecj:Y75_p0326.
eco:b0337.
PATRICi32115805. VBIEscCol129921_0344.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63656 Genomic DNA. Translation: CAA45196.1 .
S56903 Genomic DNA. Translation: AAB25761.2 .
U73857 Genomic DNA. Translation: AAB18061.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC73440.1 .
AP009048 Genomic DNA. Translation: BAE76119.1 .
PIRi S22662.
RefSeqi NP_414871.1. NC_000913.3.
YP_488631.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K6W X-ray 1.75 A 2-427 [» ]
1K70 X-ray 1.80 A 2-427 [» ]
1R9X X-ray 1.58 A 2-427 [» ]
1R9Y X-ray 1.57 A 2-427 [» ]
1R9Z X-ray 1.32 A 2-427 [» ]
1RA0 X-ray 1.12 A 2-427 [» ]
1RA5 X-ray 1.40 A 2-427 [» ]
1RAK X-ray 1.32 A 2-427 [» ]
3G77 X-ray 1.80 A 5-427 [» ]
3O7U X-ray 1.71 A 1-427 [» ]
3R0D X-ray 1.50 A 1-427 [» ]
3RN6 X-ray 2.26 A 1-427 [» ]
ProteinModelPortali P25524.
SMRi P25524. Positions 5-427.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9306N.
IntActi P25524. 6 interactions.
MINTi MINT-1307853.
STRINGi 511145.b0337.

Proteomic databases

PaxDbi P25524.
PRIDEi P25524.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73440 ; AAC73440 ; b0337 .
BAE76119 ; BAE76119 ; BAE76119 .
GeneIDi 12930819.
944996.
KEGGi ecj:Y75_p0326.
eco:b0337.
PATRICi 32115805. VBIEscCol129921_0344.

Organism-specific databases

EchoBASEi EB1302.
EcoGenei EG11326. codA.

Phylogenomic databases

eggNOGi COG0402.
HOGENOMi HOG000184778.
InParanoidi P25524.
KOi K01485.
OMAi NNINVCF.
OrthoDBi EOG6QG8F9.
PhylomeDBi P25524.

Enzyme and pathway databases

BioCyci EcoCyc:CYTDEAM-MONOMER.
ECOL316407:JW0328-MONOMER.
MetaCyc:CYTDEAM-MONOMER.
RETL1328306-WGS:GSTH-6950-MONOMER.

Miscellaneous databases

EvolutionaryTracei P25524.
PROi P25524.

Gene expression databases

Genevestigatori P25524.

Family and domain databases

Gene3Di 2.30.40.10. 1 hit.
InterProi IPR013108. Amidohydro_3.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view ]
Pfami PF07969. Amidohydro_3. 1 hit.
[Graphical view ]
SUPFAMi SSF51338. SSF51338. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the Escherichia coli codBA operon encoding cytosine permease and cytosine deaminase."
    Danielsen S., Kilstrup M., Barilla K., Jochimsen B., Neuhard J.
    Mol. Microbiol. 6:1335-1344(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20.
    Strain: CSH01.
  2. "A first step in the development of gene therapy for colorectal carcinoma: cloning, sequencing, and expression of Escherichia coli cytosine deaminase."
    Austin E.A., Huber B.E.
    Mol. Pharmacol. 43:380-387(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "The structure of Escherichia coli cytosine deaminase."
    Ireton G.C., McDermott G., Black M.E., Stoddard B.L.
    J. Mol. Biol. 315:687-697(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).

Entry informationi

Entry nameiCODA_ECOLI
AccessioniPrimary (citable) accession number: P25524
Secondary accession number(s): Q2MC87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3