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P25524 (CODA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytosine deaminase
EC=3.5.4.1
Alternative name(s):
Cytosine aminohydrolase
Gene names
Name:codA
Ordered Locus Names:b0337, JW0328
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Cytosine + H2O = uracil + NH3.

Cofactor

Iron or another divalent metal ion.

Subunit structure

Homotetramer.

Sequence caution

The sequence AAB18061.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 427426Cytosine deaminase
PRO_0000090002

Sites

Metal binding621Iron
Metal binding641Iron

Natural variations

Natural variant131R → W in strain: SO5076.

Secondary structure

1 ................................................................................ 427
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P25524 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 9F91A2C46B3B1E42

FASTA42747,591
        10         20         30         40         50         60 
MSNNALQTII NARLPGEEGL WQIHLQDGKI SAIDAQSGVM PITENSLDAE QGLVIPPFVE 

        70         80         90        100        110        120 
PHIHLDTTQT AGQPNWNQSG TLFEGIERWA ERKALLTHDD VKQRAWQTLK WQIANGIQHV 

       130        140        150        160        170        180 
RTHVDVSDAT LTALKAMLEV KQEVAPWIDL QIVAFPQEGI LSYPNGEALL EEALRLGADV 

       190        200        210        220        230        240 
VGAIPHFEFT REYGVESLHK TFALAQKYDR LIDVHCDEID DEQSRFVETV AALAHHEGMG 

       250        260        270        280        290        300 
ARVTASHTTA MHSYNGAYTS RLFRLLKMSG INFVANPLVN IHLQGRFDTY PKRRGITRVK 

       310        320        330        340        350        360 
EMLESGINVC FGHDDVFDPW YPLGTANMLQ VLHMGLHVCQ LMGYGQINDG LNLITHHSAR 

       370        380        390        400        410        420 
TLNLQDYGIA AGNSANLIIL PAENGFDALR RQVPVRYSVR GGKVIASTQP AQTTVYLEQP 


EAIDYKR

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the Escherichia coli codBA operon encoding cytosine permease and cytosine deaminase."
Danielsen S., Kilstrup M., Barilla K., Jochimsen B., Neuhard J.
Mol. Microbiol. 6:1335-1344(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20.
Strain: CSH01.
[2]"A first step in the development of gene therapy for colorectal carcinoma: cloning, sequencing, and expression of Escherichia coli cytosine deaminase."
Austin E.A., Huber B.E.
Mol. Pharmacol. 43:380-387(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"The structure of Escherichia coli cytosine deaminase."
Ireton G.C., McDermott G., Black M.E., Stoddard B.L.
J. Mol. Biol. 315:687-697(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X63656 Genomic DNA. Translation: CAA45196.1.
S56903 Genomic DNA. Translation: AAB25761.2.
U73857 Genomic DNA. Translation: AAB18061.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73440.1.
AP009048 Genomic DNA. Translation: BAE76119.1.
PIRS22662.
RefSeqNP_414871.1. NC_000913.2.
YP_488631.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K6WX-ray1.75A2-427[»]
1K70X-ray1.80A2-427[»]
1R9XX-ray1.58A3-426[»]
1R9YX-ray1.57A3-426[»]
1R9ZX-ray1.32A3-426[»]
1RA0X-ray1.12A3-426[»]
1RA5X-ray1.40A3-426[»]
1RAKX-ray1.32A3-426[»]
3G77X-ray1.80A5-427[»]
3O7UX-ray1.71A1-427[»]
3R0DX-ray1.50A1-427[»]
3RN6X-ray2.26A1-427[»]
ProteinModelPortalP25524.
SMRP25524. Positions 5-427.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-9306N.
IntActP25524. 6 interactions.
MINTMINT-1307853.
STRING511145.b0337.

Proteomic databases

PaxDbP25524.
PRIDEP25524.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73440; AAC73440; b0337.
BAE76119; BAE76119; BAE76119.
GeneID12930819.
944996.
KEGGecj:Y75_p0326.
eco:b0337.
PATRIC32115805. VBIEscCol129921_0344.

Organism-specific databases

EchoBASEEB1302.
EcoGeneEG11326. codA.

Phylogenomic databases

eggNOGCOG0402.
HOGENOMHOG000184778.
KOK01485.
OMAAKQTLKW.
ProtClustDBPRK09230.

Enzyme and pathway databases

BioCycEcoCyc:CYTDEAM-MONOMER.
ECOL316407:JW0328-MONOMER.
MetaCyc:CYTDEAM-MONOMER.

Gene expression databases

GenevestigatorP25524.

Family and domain databases

InterProIPR013108. Amidohydro_3.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PfamPF07969. Amidohydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51338. Metalo_hydrolase. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP25524.

Entry information

Entry nameCODA_ECOLI
AccessionPrimary (citable) accession number: P25524
Secondary accession number(s): Q2MC87
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: May 1, 2013
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents