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Reviewed, UniProtKB/Swiss-Prot P25524 (CODA_ECOLI)

Last modified June 16, 2009. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cytosine deaminase
    EC=3.5.4.1
Alternative name(s):
    Cytosine aminohydrolase
Gene names
Name: codA
Ordered Locus Names: b0337, JW0328
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

Cytosine + H2O = uracil + NH3.

Cofactor

Iron or another divalent metal ion.

Subunit structure

Homotetramer.

Ontologies

Keywords
   Biological processCytosine metabolism
   LigandIron
Metal-binding
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processcytosine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytosol

Inferred from direct assay. Source: UniProtKB

   Molecular functioncytosine deaminase activity

Inferred from electronic annotation. Source: EC

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 427426Cytosine deaminase
PRO_0000090002

Sites

Metal binding621Iron
Metal binding641Iron

Natural variations

Natural variant131R → W in strain: SO5076.

Secondary structure

........................................................................... 427
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P25524-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 9F91A2C46B3B1E42

FASTA42747,591
        10         20         30         40         50         60 
MSNNALQTII NARLPGEEGL WQIHLQDGKI SAIDAQSGVM PITENSLDAE QGLVIPPFVE 

        70         80         90        100        110        120 
PHIHLDTTQT AGQPNWNQSG TLFEGIERWA ERKALLTHDD VKQRAWQTLK WQIANGIQHV 

       130        140        150        160        170        180 
RTHVDVSDAT LTALKAMLEV KQEVAPWIDL QIVAFPQEGI LSYPNGEALL EEALRLGADV 

       190        200        210        220        230        240 
VGAIPHFEFT REYGVESLHK TFALAQKYDR LIDVHCDEID DEQSRFVETV AALAHHEGMG 

       250        260        270        280        290        300 
ARVTASHTTA MHSYNGAYTS RLFRLLKMSG INFVANPLVN IHLQGRFDTY PKRRGITRVK 

       310        320        330        340        350        360 
EMLESGINVC FGHDDVFDPW YPLGTANMLQ VLHMGLHVCQ LMGYGQINDG LNLITHHSAR 

       370        380        390        400        410        420 
TLNLQDYGIA AGNSANLIIL PAENGFDALR RQVPVRYSVR GGKVIASTQP AQTTVYLEQP 


EAIDYKR 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the Escherichia coli codBA operon encoding cytosine permease and cytosine deaminase."
Danielsen S., Kilstrup M., Barilla K., Jochimsen B., Neuhard J.
Mol. Microbiol. 6:1335-1344(1992) [PubMed: 1640834] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20.
Strain: CSH01.
[2]"A first step in the development of gene therapy for colorectal carcinoma: cloning, sequencing, and expression of Escherichia coli cytosine deaminase."
Austin E.A., Huber B.E.
Mol. Pharmacol. 43:380-387(1993) [PubMed: 8450832] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"The structure of Escherichia coli cytosine deaminase."
Ireton G.C., McDermott G., Black M.E., Stoddard B.L.
J. Mol. Biol. 315:687-697(2002) [PubMed: 11812140] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

X63656 Genomic DNA. Translation: CAA45196.1.
S56903 Genomic DNA. Translation: AAB25761.2.
U73857 Genomic DNA. Translation: AAB18061.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73440.1.
AP009048 Genomic DNA. Translation: BAE76119.1.
PIRS22662.
RefSeqAP_000989.1.
NP_414871.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1K6WX-ray1.75A2-427[»]
1K70X-ray1.80A2-427[»]
1R9XX-ray1.58A3-426[»]
1R9YX-ray1.57A3-426[»]
1R9ZX-ray1.32A3-426[»]
1RA0X-ray1.12A3-426[»]
1RA5X-ray1.40A3-426[»]
1RAKX-ray1.32A3-426[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:9306N.

Genome annotation databases

GeneID944996.
GenomeReviewsGene locus JW0328 in contig AP009048_GR.
Gene locus b0337 in contig U00096_GR.
KEGGecj:JW0328.
eco:b0337.

Organism-specific databases

EchoBASEEB1302.
EcoGeneEG11326. codA.
CMRSearch...

Phylogenomic databases

HOGENOMP25524.
OMAP25524. AKQTLKW.

Enzyme and pathway databases

BioCycEcoCyc:CYTDEAM-MON.
MetaCyc:CYTDEAM-MON.

Family and domain databases

InterProIPR013108. Amidohydro_3.
[Graphical view]
PfamPF07969. Amidohydro_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCODA_ECOLI
AccessionPrimary (citable) accession number: P25524
Secondary accession number(s): Q2MC87
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents