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Protein

GTPase HflX

Gene

hflX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis. In vitro, also exhibits ATPase activity.UniRule annotation3 Publications

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Intrinsic GTPase activity is very slow and can be stimulated by the presence of 50S ribosomal subunits or 70S ribosomes. GTPase activity is inhibited by ATP.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi211MagnesiumUniRule annotation1
Metal bindingi231MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi204 – 211GTPUniRule annotation8
Nucleotide bindingi229 – 233GTPUniRule annotation5
Nucleotide bindingi251 – 254GTPUniRule annotation4
Nucleotide bindingi317 – 320GTPUniRule annotation4
Nucleotide bindingi343 – 345GTPUniRule annotation3

GO - Molecular functioni

  • ATPase activity Source: EcoCyc
  • ATP binding Source: EcoliWiki
  • GTPase activity Source: EcoCyc
  • GTP binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • ribosomal large subunit binding Source: EcoCyc
  • ribosome binding Source: EcoCyc
  • rRNA binding Source: EcoCyc

GO - Biological processi

  • peptidyl-serine autophosphorylation Source: EcoCyc
  • rescue of stalled ribosome Source: EcoCyc
  • response to heat Source: EcoCyc
  • ribosome disassembly Source: EcoCyc

Keywordsi

LigandATP-binding, GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10437-MONOMER
MetaCyc:EG10437-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase HflXUniRule annotation
Alternative name(s):
GTP-binding protein HflXUniRule annotation
Gene namesi
Name:hflXUniRule annotation
Ordered Locus Names:b4173, JW4131
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10437 hflX

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication
  • Note: May associate with membranes.

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Disruption does not affect lambda lysogeny or the transposition frequency of transposable elements.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224631 – 426GTPase HflXAdd BLAST426

Proteomic databases

PaxDbiP25519
PRIDEiP25519

Interactioni

Subunit structurei

Monomer. Associates with the 50S ribosomal subunit. This interaction occurs in the presence of GTP, GDP, ATP or ADP, but not in their absence.UniRule annotation2 Publications

Protein-protein interaction databases

BioGridi4261252, 369 interactors
DIPiDIP-9895N
IntActiP25519, 1 interactor
STRINGi316385.ECDH10B_4368

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5ADYelectron microscopy4.5061-426[»]
ProteinModelPortaliP25519
SMRiP25519
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini198 – 365Hflx-type GUniRule annotationAdd BLAST168

Domaini

Full-length protein is required for specific association with the 50S ribosomal subunit.1 Publication

Sequence similaritiesi

Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1N Bacteria
COG2262 LUCA
HOGENOMiHOG000260368
InParanoidiP25519
KOiK03665
OMAiHPATYIG
PhylomeDBiP25519

Family and domain databases

CDDicd01878 HflX, 1 hit
HAMAPiMF_00900 GTPase_HflX, 1 hit
InterProiView protein in InterPro
IPR035647 EFG_III/V
IPR030394 G_HFLX_dom
IPR032305 GTP-bd_M
IPR006073 GTP_binding_domain
IPR016496 GTPase_HflX
IPR025121 GTPase_HflX_N
IPR027417 P-loop_NTPase
PANTHERiPTHR10229 PTHR10229, 1 hit
PfamiView protein in Pfam
PF16360 GTP-bdg_M, 1 hit
PF13167 GTP-bdg_N, 1 hit
PF01926 MMR_HSR1, 1 hit
PIRSFiPIRSF006809 GTP-binding_hflX_prd, 1 hit
PRINTSiPR00326 GTP1OBG
SUPFAMiSSF52540 SSF52540, 1 hit
SSF54980 SSF54980, 1 hit
TIGRFAMsiTIGR03156 GTP_HflX, 1 hit
PROSITEiView protein in PROSITE
PS51705 G_HFLX, 1 hit

Sequencei

Sequence statusi: Complete.

P25519-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDRYDAGEQ AVLVHIYFTQ DKDMEDLQEF ESLVSSAGVE ALQVITGSRK
60 70 80 90 100
APHPKYFVGE GKAVEIAEAV KATGASVVLF DHALSPAQER NLERLCECRV
110 120 130 140 150
IDRTGLILDI FAQRARTHEG KLQVELAQLR HLATRLVRGW THLERQKGGI
160 170 180 190 200
GLRGPGETQL ETDRRLLRNR IVQIQSRLER VEKQREQGRQ SRIKADVPTV
210 220 230 240 250
SLVGYTNAGK STLFNRITEA RVYAADQLFA TLDPTLRRID VADVGETVLA
260 270 280 290 300
DTVGFIRHLP HDLVAAFKAT LQETRQATLL LHVIDAADVR VQENIEAVNT
310 320 330 340 350
VLEEIDAHEI PTLLVMNKID MLEDFEPRID RDEENKPNRV WLSAQTGAGI
360 370 380 390 400
PQLFQALTER LSGEVAQHTL RLPPQEGRLR SRFYQLQAIE KEWMEEDGSV
410 420
SLQVRMPIVD WRRLCKQEPA LIDYLI
Length:426
Mass (Da):48,327
Last modified:February 1, 1994 - v3
Checksum:i8D0A5BE92EE32591
GO

Sequence cautioni

The sequence BAA00645 differs from that shown. Reason: Frameshift at position 18.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53H → R in BAA00645 (PubMed:2020545).Curated1
Sequence conflicti56Y → S in BAA00645 (PubMed:2020545).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00005 Unassigned DNA Translation: AAC43398.1
U14003 Genomic DNA Translation: AAA97069.1
U00096 Genomic DNA Translation: AAC77130.1
AP009048 Genomic DNA Translation: BAE78174.1
D00743 Genomic DNA Translation: BAA00645.1 Frameshift.
PIRiS56398
RefSeqiNP_418594.1, NC_000913.3
WP_000460362.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77130; AAC77130; b4173
BAE78174; BAE78174; BAE78174
GeneIDi948688
KEGGiecj:JW4131
eco:b4173
PATRICifig|1411691.4.peg.2528

Similar proteinsi

Entry informationi

Entry nameiHFLX_ECOLI
AccessioniPrimary (citable) accession number: P25519
Secondary accession number(s): Q2M6D2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 139 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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