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Protein

GTPase HflX

Gene

hflX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis. In vitro, also exhibits ATPase activity.UniRule annotation3 Publications

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Intrinsic GTPase activity is very slow and can be stimulated by the presence of 50S ribosomal subunits or 70S ribosomes. GTPase activity is inhibited by ATP.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi211MagnesiumUniRule annotation1
Metal bindingi231MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi204 – 211GTPUniRule annotation8
Nucleotide bindingi229 – 233GTPUniRule annotation5
Nucleotide bindingi251 – 254GTPUniRule annotation4
Nucleotide bindingi317 – 320GTPUniRule annotation4
Nucleotide bindingi343 – 345GTPUniRule annotation3

GO - Molecular functioni

  • ATP binding Source: EcoliWiki
  • GTP binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • ribosome binding Source: EcoCyc
  • rRNA binding Source: EcoCyc

GO - Biological processi

  • response to heat Source: EcoliWiki

Keywordsi

LigandATP-binding, GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10437-MONOMER.
MetaCyc:EG10437-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase HflXUniRule annotation
Alternative name(s):
GTP-binding protein HflXUniRule annotation
Gene namesi
Name:hflXUniRule annotation
Ordered Locus Names:b4173, JW4131
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10437. hflX.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

  • Note: May associate with membranes.

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Disruption does not affect lambda lysogeny or the transposition frequency of transposable elements.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224631 – 426GTPase HflXAdd BLAST426

Proteomic databases

PaxDbiP25519.
PRIDEiP25519.

Interactioni

Subunit structurei

Monomer. Associates with the 50S ribosomal subunit. This interaction occurs in the presence of GTP, GDP, ATP or ADP, but not in their absence.UniRule annotation2 Publications

Protein-protein interaction databases

BioGridi4261252. 360 interactors.
DIPiDIP-9895N.
IntActiP25519. 1 interactor.
STRINGi316385.ECDH10B_4368.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5ADYelectron microscopy4.5061-426[»]
ProteinModelPortaliP25519.
SMRiP25519.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini198 – 365Hflx-type GUniRule annotationAdd BLAST168

Domaini

Full-length protein is required for specific association with the 50S ribosomal subunit.1 Publication

Sequence similaritiesi

Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1N. Bacteria.
COG2262. LUCA.
HOGENOMiHOG000260368.
InParanoidiP25519.
KOiK03665.
PhylomeDBiP25519.

Family and domain databases

CDDicd01878. HflX. 1 hit.
HAMAPiMF_00900. GTPase_HflX. 1 hit.
InterProiView protein in InterPro
IPR009022. EFG_III-V.
IPR030394. G_HFLX_dom.
IPR032305. GTP-bd_M.
IPR006073. GTP_binding_domain.
IPR016496. GTPase_HflX.
IPR025121. GTPase_HflX_N.
IPR027417. P-loop_NTPase.
PANTHERiPTHR10229. PTHR10229. 1 hit.
PfamiView protein in Pfam
PF16360. GTP-bdg_M. 1 hit.
PF13167. GTP-bdg_N. 1 hit.
PF01926. MMR_HSR1. 1 hit.
PIRSFiPIRSF006809. GTP-binding_hflX_prd. 1 hit.
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 1 hit.
TIGRFAMsiTIGR03156. GTP_HflX. 1 hit.
PROSITEiView protein in PROSITE
PS51705. G_HFLX. 1 hit.

Sequencei

Sequence statusi: Complete.

P25519-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDRYDAGEQ AVLVHIYFTQ DKDMEDLQEF ESLVSSAGVE ALQVITGSRK
60 70 80 90 100
APHPKYFVGE GKAVEIAEAV KATGASVVLF DHALSPAQER NLERLCECRV
110 120 130 140 150
IDRTGLILDI FAQRARTHEG KLQVELAQLR HLATRLVRGW THLERQKGGI
160 170 180 190 200
GLRGPGETQL ETDRRLLRNR IVQIQSRLER VEKQREQGRQ SRIKADVPTV
210 220 230 240 250
SLVGYTNAGK STLFNRITEA RVYAADQLFA TLDPTLRRID VADVGETVLA
260 270 280 290 300
DTVGFIRHLP HDLVAAFKAT LQETRQATLL LHVIDAADVR VQENIEAVNT
310 320 330 340 350
VLEEIDAHEI PTLLVMNKID MLEDFEPRID RDEENKPNRV WLSAQTGAGI
360 370 380 390 400
PQLFQALTER LSGEVAQHTL RLPPQEGRLR SRFYQLQAIE KEWMEEDGSV
410 420
SLQVRMPIVD WRRLCKQEPA LIDYLI
Length:426
Mass (Da):48,327
Last modified:February 1, 1994 - v3
Checksum:i8D0A5BE92EE32591
GO

Sequence cautioni

The sequence BAA00645 differs from that shown. Reason: Frameshift at position 18.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53H → R in BAA00645 (PubMed:2020545).Curated1
Sequence conflicti56Y → S in BAA00645 (PubMed:2020545).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00005 Unassigned DNA. Translation: AAC43398.1.
U14003 Genomic DNA. Translation: AAA97069.1.
U00096 Genomic DNA. Translation: AAC77130.1.
AP009048 Genomic DNA. Translation: BAE78174.1.
D00743 Genomic DNA. Translation: BAA00645.1. Frameshift.
PIRiS56398.
RefSeqiNP_418594.1. NC_000913.3.
WP_000460362.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77130; AAC77130; b4173.
BAE78174; BAE78174; BAE78174.
GeneIDi948688.
KEGGiecj:JW4131.
eco:b4173.
PATRICifig|1411691.4.peg.2528.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiHFLX_ECOLI
AccessioniPrimary (citable) accession number: P25519
Secondary accession number(s): Q2M6D2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1994
Last modified: July 5, 2017
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families