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Protein

V-type proton ATPase subunit c

Gene

VMA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. It is an electrogenic proton pump that generates a proton motive force of 180 mv, inside positive and acidic, in the vacuolar membrane vesicles.

Miscellaneous

Present with 8450 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei137Essential for proton translocation1

GO - Molecular functioni

GO - Biological processi

  • ATP hydrolysis coupled proton transport Source: InterPro
  • cellular copper ion homeostasis Source: SGD
  • cellular iron ion homeostasis Source: SGD
  • endocytosis Source: SGD
  • protein targeting to vacuole Source: SGD
  • proton transport Source: SGD
  • vacuolar acidification Source: SGD
  • vacuole organization Source: SGD

Keywordsi

Biological processHydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30150-MONOMER.
ReactomeiR-SCE-1222556. ROS, RNS production in phagocytes.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-77387. Insulin receptor recycling.
R-SCE-917977. Transferrin endocytosis and recycling.

Protein family/group databases

TCDBi3.A.2.2.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase subunit c
Short name:
V-ATPase subunit c
Alternative name(s):
Guanine nucleotide exchange factor 2
V-ATPase 16 kDa proteolipid subunit 1
Vacuolar proton pump c subunit
Gene namesi
Name:VMA3
Synonyms:CLS7, CUP5, GEF2
Ordered Locus Names:YEL027W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL027W.
SGDiS000000753. VMA3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8VacuolarSequence analysis8
Transmembranei9 – 31HelicalSequence analysisAdd BLAST23
Topological domaini32 – 53CytoplasmicSequence analysisAdd BLAST22
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Topological domaini75 – 90VacuolarSequence analysisAdd BLAST16
Transmembranei91 – 112HelicalSequence analysisAdd BLAST22
Topological domaini113 – 124CytoplasmicSequence analysisAdd BLAST12
Transmembranei125 – 150HelicalSequence analysisAdd BLAST26
Topological domaini151 – 160VacuolarSequence analysis10

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi137E → D: Partial inactivation. 1 Publication1
Mutagenesisi137E → Q, V or K: Inactivation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000717621 – 160V-type proton ATPase subunit cAdd BLAST160

Proteomic databases

MaxQBiP25515.
PRIDEiP25515.
TopDownProteomicsiP25515.

Interactioni

Subunit structurei

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d and e). The proteolipid components c, c' and c'' are present as a hexameric ring that forms the proton-conducting pore.

Binary interactionsi

WithEntry#Exp.IntActNotes
UBE2IP632793EBI-7739233,EBI-80168From Homo sapiens.

Protein-protein interaction databases

BioGridi36702. 385 interactors.
DIPiDIP-8123N.
IntActiP25515. 8 interactors.
MINTiMINT-1353979.
STRINGi4932.YEL027W.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J9Telectron microscopy6.90R/S/T/U/V/W/X/Y/Z/a1-160[»]
3J9Uelectron microscopy7.60R/S/T/U/V/W/X/Y/Z/a1-160[»]
3J9Velectron microscopy8.30R/S/T/U/V/W/X/Y/Z/a1-160[»]
5TJ5electron microscopy3.90E/F/G/H/I/J/M/N9-158[»]
5VOXelectron microscopy6.80T/U/V/W/X/Y/Z/a1-160[»]
5VOYelectron microscopy7.90T/U/V/W/X/Y/Z/a1-160[»]
ProteinModelPortaliP25515.
SMRiP25515.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074873.
HOGENOMiHOG000056520.
InParanoidiP25515.
KOiK02155.
OMAiTCFGASY.
OrthoDBiEOG092C5DS5.

Family and domain databases

InterProiView protein in InterPro
IPR002379. ATPase_proteolipid_c-like_dom.
IPR000245. ATPase_proteolipid_csu.
IPR011555. ATPase_proteolipid_su_C_euk.
PfamiView protein in Pfam
PF00137. ATP-synt_C. 2 hits.
PRINTSiPR00122. VACATPASE.
SUPFAMiSSF81333. SSF81333. 2 hits.
TIGRFAMsiTIGR01100. V_ATP_synt_C. 1 hit.

Sequencei

Sequence statusi: Complete.

P25515-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELCPVYAP FFGAIGCASA IIFTSLGAAY GTAKSGVGIC ATCVLRPDLL
60 70 80 90 100
FKNIVPVIMA GIIAIYGLVV SVLVCYSLGQ KQALYTGFIQ LGAGLSVGLS
110 120 130 140 150
GLAAGFAIGI VGDAGVRGSS QQPRLFVGMI LILIFAEVLG LYGLIVALLL
160
NSRATQDVVC
Length:160
Mass (Da):16,351
Last modified:May 1, 1992 - v1
Checksum:iFB8A142FFA1FA964
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15155 mRNA. Translation: CAA33249.1.
U18530 Genomic DNA. Translation: AAB64504.1.
AY558342 Genomic DNA. Translation: AAS56668.1.
BK006939 Genomic DNA. Translation: DAA07625.1.
PIRiS22257. PXBYL6.
RefSeqiNP_010887.3. NM_001178842.3.

Genome annotation databases

EnsemblFungiiYEL027W; YEL027W; YEL027W.
GeneIDi856686.
KEGGisce:YEL027W.

Similar proteinsi

Entry informationi

Entry nameiVATL1_YEAST
AccessioniPrimary (citable) accession number: P25515
Secondary accession number(s): D3DLM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 27, 2017
This is version 168 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names