Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proline utilization trans-activator

Gene

PUT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Positive activator of the proline utilization pathway. Binds to the promoters of PUT1 and PUT2 genes. Recognizes and binds to the DNA sequence 5'-CGG-N(10)-CCG-3'.

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Zinc 11
Metal bindingi34Zinc 21
Metal bindingi37Zinc 11
Metal bindingi44Zinc 11
Metal bindingi50Zinc 11
Metal bindingi50Zinc 21
Metal bindingi53Zinc 21
Metal bindingi60Zinc 21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi34 – 60Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd BLAST27

GO - Molecular functioni

GO - Biological processi

  • positive regulation of proline catabolic process to glutamate Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • proline metabolic process Source: UniProtKB-KW
  • transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD

Keywordsi

Molecular functionActivator, DNA-binding
Biological processProline metabolism, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31824-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Proline utilization trans-activator
Gene namesi
Name:PUT3
Ordered Locus Names:YKL015W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL015W
SGDiS000001498 PUT3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149681 – 979Proline utilization trans-activatorAdd BLAST979

Proteomic databases

MaxQBiP25502
PaxDbiP25502
PRIDEiP25502

PTM databases

iPTMnetiP25502

Interactioni

Subunit structurei

Binds DNA as a homodimer.

Protein-protein interaction databases

BioGridi34117, 103 interactors
DIPiDIP-1425N
IntActiP25502, 5 interactors
MINTiP25502
STRINGi4932.YKL015W

Structurei

Secondary structure

1979
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 40Combined sources6
Beta strandi47 – 49Combined sources3
Helixi51 – 55Combined sources5
Beta strandi68 – 72Combined sources5
Helixi73 – 96Combined sources24

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AJYNMR-A/B31-100[»]
1ZMEX-ray2.50C/D31-100[»]
ProteinModelPortaliP25502
SMRiP25502
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25502

Family & Domainsi

Phylogenomic databases

GeneTreeiENSGT00530000068025
HOGENOMiHOG000248496
InParanoidiP25502
KOiK09248
OMAiLILGWHV
OrthoDBiEOG092C14SA

Family and domain databases

CDDicd00067 GAL4, 1 hit
Gene3Di4.10.240.10, 1 hit
InterProiView protein in InterPro
IPR007219 Transcription_factor_dom_fun
IPR001138 Zn2-C6_fun-type_DNA-bd
IPR036864 Zn2-C6_fun-type_DNA-bd_sf
PfamiView protein in Pfam
PF04082 Fungal_trans, 1 hit
PF00172 Zn_clus, 1 hit
SMARTiView protein in SMART
SM00906 Fungal_trans, 1 hit
SM00066 GAL4, 1 hit
SUPFAMiSSF57701 SSF57701, 1 hit
PROSITEiView protein in PROSITE
PS00463 ZN2_CY6_FUNGAL_1, 1 hit
PS50048 ZN2_CY6_FUNGAL_2, 1 hit

Sequencei

Sequence statusi: Complete.

P25502-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTDQGSRHS IQSKQPAYVN KQPQKRQQRS SVACLSCRKR HIKCPGGNPC
60 70 80 90 100
QKCVTSNAIC EYLEPSKKIV VSTKYLQQLQ KDLNDKTEEN NRLKALLLER
110 120 130 140 150
PVSVRGKDNS DDDERHINNA PSSDTLEVSS APAAPIFDLM SNSNTASDND
160 170 180 190 200
NDDDNSNRIT NNRSYDHSLE KYYKKAISIF KQPANANGEN GNGANGHEDD
210 220 230 240 250
DEDDEEISTN FAQRSGRLIE SHNGFHYFVG SSSMTLFGLE IQSLVTKYIS
260 270 280 290 300
VKNFRPLPIN TKNKILNSNL NPAISSFINS NNYLFSSYNF LNPISTIVNL
310 320 330 340 350
NSINDNLSPL MFKIILKSDT DGSSGQEEVI QFQLPSYNYT KLLIDCFINY
360 370 380 390 400
NDGCFYFFNE GLVKCGINKL YLENKWLYYD NTKKALDNEN DPILQAVWFC
410 420 430 440 450
KILLILAVGE MYLGSINNEM LKNYSNQPKL PGSKFFQMGS KIFNCLFSSE
460 470 480 490 500
RLENVTKKGG IEVLLLYAFF LQVADYTLAS YFYFGQALRT CLILGLHVDS
510 520 530 540 550
QSDTLSRYEI EHHRRLWWTV YMFERMLSSK AGLPLSFTDY TISTALPADI
560 570 580 590 600
DDETIEEKNS HYVFRKAELI SNCVTIVKIN AQILSKLYQR QPETNIIITL
610 620 630 640 650
KVVIKQLLEW RNNLSDSLQV DFTQKDEDFK ISRLSTNMFT EYFQGINLAV
660 670 680 690 700
RPLLFHFASI QLKRFKTSNT FVNLQNYSAT ISSLLTCSLH ASVNTIRSLW
710 720 730 740 750
SLLQNSMLAM FSYMDREYLF TSSCTLLLFN TAFGIHEQTL YHLDHSLEIF
760 770 780 790 800
TQMRNLGNIP AGLRRAQLLT LMANLDFHGI MNDLITKYND ILKFDSMNCE
810 820 830 840 850
NDNIVEDSNE PKRETEKCKP HKDGDRIDPS IIDCDKSNTN TNMIKNESIS
860 870 880 890 900
NIVSILPEGA KPTLTDYSNG NNDVNDINVN NSEPSTFFDI ITASLENSYQ
910 920 930 940 950
TTLTEKGSQV MEKNMDQLDS VHNLNDDDLQ QLLEDLGNID HSDEKLWKEI
960 970
TDQAMWLGNT MDPTAAAGSE IDFTDYLGP
Length:979
Mass (Da):111,414
Last modified:May 1, 1992 - v1
Checksum:i59FA19EFC79EE0C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55384 Genomic DNA Translation: CAA39055.1
X74152 Genomic DNA Translation: CAA52267.1
Z28015 Genomic DNA Translation: CAA81850.1
BK006944 Genomic DNA Translation: DAA09141.1
PIRiA39792
RefSeqiNP_012910.1, NM_001179581.1

Genome annotation databases

EnsemblFungiiYKL015W; YKL015W; YKL015W
GeneIDi853854
KEGGisce:YKL015W

Similar proteinsi

Entry informationi

Entry nameiPUT3_YEAST
AccessioniPrimary (citable) accession number: P25502
Secondary accession number(s): D6VXS1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health