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Protein

Proline utilization trans-activator

Gene

PUT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Positive activator of the proline utilization pathway. Binds to the promoters of PUT1 and PUT2 genes. Recognizes and binds to the DNA sequence 5'-CGG-N(10)-CCG-3'.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Zinc 1
Metal bindingi34 – 341Zinc 2
Metal bindingi37 – 371Zinc 1
Metal bindingi44 – 441Zinc 1
Metal bindingi50 – 501Zinc 1
Metal bindingi50 – 501Zinc 2
Metal bindingi53 – 531Zinc 2
Metal bindingi60 – 601Zinc 2

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi34 – 6027Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • positive regulation of proline catabolic process to glutamate Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • proline metabolic process Source: UniProtKB-KW
  • transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Proline metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31824-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline utilization trans-activator
Gene namesi
Name:PUT3
Ordered Locus Names:YKL015W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL015W.
SGDiS000001498. PUT3.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 979979Proline utilization trans-activatorPRO_0000114968Add
BLAST

Proteomic databases

MaxQBiP25502.

PTM databases

iPTMnetiP25502.

Interactioni

Subunit structurei

Binds DNA as a homodimer.

Protein-protein interaction databases

BioGridi34117. 33 interactions.
DIPiDIP-1425N.
IntActiP25502. 5 interactions.
MINTiMINT-394993.

Structurei

Secondary structure

1
979
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi35 – 406Combined sources
Beta strandi47 – 493Combined sources
Helixi51 – 555Combined sources
Beta strandi68 – 725Combined sources
Helixi73 – 9624Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AJYNMR-A/B31-100[»]
1ZMEX-ray2.50C/D31-100[»]
ProteinModelPortaliP25502.
SMRiP25502. Positions 31-100.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25502.

Family & Domainsi

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000068025.
HOGENOMiHOG000248496.
InParanoidiP25502.
KOiK09248.
OMAiCEYVEAD.
OrthoDBiEOG7WT48X.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR007219. Transcription_factor_dom_fun.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF04082. Fungal_trans. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00906. Fungal_trans. 1 hit.
SM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25502-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTDQGSRHS IQSKQPAYVN KQPQKRQQRS SVACLSCRKR HIKCPGGNPC
60 70 80 90 100
QKCVTSNAIC EYLEPSKKIV VSTKYLQQLQ KDLNDKTEEN NRLKALLLER
110 120 130 140 150
PVSVRGKDNS DDDERHINNA PSSDTLEVSS APAAPIFDLM SNSNTASDND
160 170 180 190 200
NDDDNSNRIT NNRSYDHSLE KYYKKAISIF KQPANANGEN GNGANGHEDD
210 220 230 240 250
DEDDEEISTN FAQRSGRLIE SHNGFHYFVG SSSMTLFGLE IQSLVTKYIS
260 270 280 290 300
VKNFRPLPIN TKNKILNSNL NPAISSFINS NNYLFSSYNF LNPISTIVNL
310 320 330 340 350
NSINDNLSPL MFKIILKSDT DGSSGQEEVI QFQLPSYNYT KLLIDCFINY
360 370 380 390 400
NDGCFYFFNE GLVKCGINKL YLENKWLYYD NTKKALDNEN DPILQAVWFC
410 420 430 440 450
KILLILAVGE MYLGSINNEM LKNYSNQPKL PGSKFFQMGS KIFNCLFSSE
460 470 480 490 500
RLENVTKKGG IEVLLLYAFF LQVADYTLAS YFYFGQALRT CLILGLHVDS
510 520 530 540 550
QSDTLSRYEI EHHRRLWWTV YMFERMLSSK AGLPLSFTDY TISTALPADI
560 570 580 590 600
DDETIEEKNS HYVFRKAELI SNCVTIVKIN AQILSKLYQR QPETNIIITL
610 620 630 640 650
KVVIKQLLEW RNNLSDSLQV DFTQKDEDFK ISRLSTNMFT EYFQGINLAV
660 670 680 690 700
RPLLFHFASI QLKRFKTSNT FVNLQNYSAT ISSLLTCSLH ASVNTIRSLW
710 720 730 740 750
SLLQNSMLAM FSYMDREYLF TSSCTLLLFN TAFGIHEQTL YHLDHSLEIF
760 770 780 790 800
TQMRNLGNIP AGLRRAQLLT LMANLDFHGI MNDLITKYND ILKFDSMNCE
810 820 830 840 850
NDNIVEDSNE PKRETEKCKP HKDGDRIDPS IIDCDKSNTN TNMIKNESIS
860 870 880 890 900
NIVSILPEGA KPTLTDYSNG NNDVNDINVN NSEPSTFFDI ITASLENSYQ
910 920 930 940 950
TTLTEKGSQV MEKNMDQLDS VHNLNDDDLQ QLLEDLGNID HSDEKLWKEI
960 970
TDQAMWLGNT MDPTAAAGSE IDFTDYLGP
Length:979
Mass (Da):111,414
Last modified:May 1, 1992 - v1
Checksum:i59FA19EFC79EE0C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55384 Genomic DNA. Translation: CAA39055.1.
X74152 Genomic DNA. Translation: CAA52267.1.
Z28015 Genomic DNA. Translation: CAA81850.1.
BK006944 Genomic DNA. Translation: DAA09141.1.
PIRiA39792.
RefSeqiNP_012910.1. NM_001179581.1.

Genome annotation databases

EnsemblFungiiYKL015W; YKL015W; YKL015W.
GeneIDi853854.
KEGGisce:YKL015W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55384 Genomic DNA. Translation: CAA39055.1.
X74152 Genomic DNA. Translation: CAA52267.1.
Z28015 Genomic DNA. Translation: CAA81850.1.
BK006944 Genomic DNA. Translation: DAA09141.1.
PIRiA39792.
RefSeqiNP_012910.1. NM_001179581.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AJYNMR-A/B31-100[»]
1ZMEX-ray2.50C/D31-100[»]
ProteinModelPortaliP25502.
SMRiP25502. Positions 31-100.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34117. 33 interactions.
DIPiDIP-1425N.
IntActiP25502. 5 interactions.
MINTiMINT-394993.

PTM databases

iPTMnetiP25502.

Proteomic databases

MaxQBiP25502.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL015W; YKL015W; YKL015W.
GeneIDi853854.
KEGGisce:YKL015W.

Organism-specific databases

EuPathDBiFungiDB:YKL015W.
SGDiS000001498. PUT3.

Phylogenomic databases

GeneTreeiENSGT00530000068025.
HOGENOMiHOG000248496.
InParanoidiP25502.
KOiK09248.
OMAiCEYVEAD.
OrthoDBiEOG7WT48X.

Enzyme and pathway databases

BioCyciYEAST:G3O-31824-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP25502.
PROiP25502.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR007219. Transcription_factor_dom_fun.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF04082. Fungal_trans. 1 hit.
PF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00906. Fungal_trans. 1 hit.
SM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of constitutive and noninducible mutations of the PUT3 transcriptional activator."
    Marczak J.E., Brandriss M.C.
    Mol. Cell. Biol. 11:2609-2619(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene."
    Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.
    Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: STRUCTURE BY NMR OF 30-100.
  7. "Crystal structure of a PUT3-DNA complex reveals a novel mechanism for DNA recognition by a protein containing a Zn2Cys6 binuclear cluster."
    Swaminathan K., Flynn P., Reece R.J., Marmorstein R.
    Nat. Struct. Biol. 4:751-759(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 31-100.

Entry informationi

Entry nameiPUT3_YEAST
AccessioniPrimary (citable) accession number: P25502
Secondary accession number(s): D6VXS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: July 6, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.