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Protein

Mitochondrial protein import protein MAS5

Gene

YDJ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in mitochondrial protein import. Is also required for efficient translocation of pre-pro-alpha-factor. Involved in heme regulation of HAP1, as a component of the high-molecular-weight (HMC) complex.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116Substrate; via amide nitrogen1
Metal bindingi143Zinc 11
Metal bindingi146Zinc 11
Metal bindingi159Zinc 21
Metal bindingi162Zinc 21
Metal bindingi185Zinc 21
Metal bindingi188Zinc 21
Metal bindingi201Zinc 11
Metal bindingi204Zinc 11
Sitei335Involved in dimerization1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri130 – 213CR-typeAdd BLAST84

GO - Molecular functioni

  • ATPase activator activity Source: SGD
  • ATP binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • unfolded protein binding Source: SGD

GO - Biological processi

  • 'de novo' protein folding Source: SGD
  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • protein refolding Source: SGD
  • protein targeting to ER Source: SGD
  • protein targeting to mitochondrion Source: SGD
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
  • response to heat Source: InterPro
  • tRNA import into nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Stress response, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33094-MONOMER.
ReactomeiR-SCE-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial protein import protein MAS5
Alternative name(s):
Yeast dnaJ protein 1
Gene namesi
Name:YDJ1
Synonyms:MAS5
Ordered Locus Names:YNL064C
ORF Names:N2418, YNL2418C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL064C.
SGDiS000005008. YDJ1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • perinuclear region of cytoplasm Source: SGD
  • TRC complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi335F → D: Prevents dimerization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000710931 – 406Mitochondrial protein import protein MAS5Add BLAST406
PropeptideiPRO_0000396684407 – 409Removed in mature formCurated3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki198Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei406Cysteine methyl esterCurated1
Lipidationi406S-farnesyl cysteine1 Publication1

Keywords - PTMi

Isopeptide bond, Lipoprotein, Methylation, Prenylation, Ubl conjugation

Proteomic databases

MaxQBiP25491.
PRIDEiP25491.

PTM databases

iPTMnetiP25491.

Expressioni

Inductioni

YDJ1 is a heat shock gene whose expression increases moderately at elevated temperatures.

Interactioni

Subunit structurei

Homodimer. Interacts with HAP1. Component of the HMC including HAP1, SRO9 and YDJ1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MDY2Q122852EBI-10420,EBI-34904
SGT2Q121182EBI-10420,EBI-31784
SUP35Q7LKB13EBI-10420,EBI-8411471From a different organism.

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi35759. 495 interactors.
DIPiDIP-2251N.
IntActiP25491. 16 interactors.
MINTiMINT-603538.

Structurei

Secondary structure

1409
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi116 – 122Combined sources7
Helixi126 – 129Combined sources4
Beta strandi131 – 142Combined sources12
Turni144 – 148Combined sources5
Beta strandi150 – 152Combined sources3
Turni153 – 155Combined sources3
Beta strandi162 – 166Combined sources5
Beta strandi168 – 182Combined sources15
Beta strandi189 – 193Combined sources5
Beta strandi202 – 204Combined sources3
Beta strandi209 – 220Combined sources12
Beta strandi229 – 232Combined sources4
Beta strandi247 – 253Combined sources7
Beta strandi260 – 262Combined sources3
Beta strandi265 – 273Combined sources9
Helixi274 – 279Combined sources6
Beta strandi281 – 286Combined sources6
Beta strandi288 – 290Combined sources3
Beta strandi292 – 297Combined sources6
Turni299 – 302Combined sources4
Beta strandi308 – 311Combined sources4
Beta strandi319 – 321Combined sources3
Beta strandi327 – 334Combined sources8
Helixi343 – 352Combined sources10
Beta strandi367 – 371Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NLTX-ray2.70A103-350[»]
1XAOX-ray2.07A/B258-378[»]
ProteinModelPortaliP25491.
SMRiP25491.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25491.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 72JAdd BLAST69
Repeati143 – 150CXXCXGXG motif8
Repeati159 – 166CXXCXGXG motif8
Repeati185 – 192CXXCXGXG motif8
Repeati201 – 208CXXCXGXG motif8

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 172Necessary for HAP1 repression in the absence of hemeAdd BLAST172
Regioni135 – 137Substrate binding3
Regioni215 – 216Substrate binding2
Regioni247 – 249Substrate binding3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi73 – 103Gly-richAdd BLAST31

Sequence similaritiesi

Contains 1 CR-type zinc finger.Curated
Contains 1 J domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri130 – 213CR-typeAdd BLAST84

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00860000133716.
HOGENOMiHOG000226718.
InParanoidiP25491.
KOiK09503.
OMAiIKHEISA.
OrthoDBiEOG092C1UGR.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25491-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKETKFYDI LGVPVTATDV EIKKAYRKCA LKYHPDKNPS EEAAEKFKEA
60 70 80 90 100
SAAYEILSDP EKRDIYDQFG EDGLSGAGGA GGFPGGGFGF GDDIFSQFFG
110 120 130 140 150
AGGAQRPRGP QRGKDIKHEI SASLEELYKG RTAKLALNKQ ILCKECEGRG
160 170 180 190 200
GKKGAVKKCT SCNGQGIKFV TRQMGPMIQR FQTECDVCHG TGDIIDPKDR
210 220 230 240 250
CKSCNGKKVE NERKILEVHV EPGMKDGQRI VFKGEADQAP DVIPGDVVFI
260 270 280 290 300
VSERPHKSFK RDGDDLVYEA EIDLLTAIAG GEFALEHVSG DWLKVGIVPG
310 320 330 340 350
EVIAPGMRKV IEGKGMPIPK YGGYGNLIIK FTIKFPENHF TSEENLKKLE
360 370 380 390 400
EILPPRIVPA IPKKATVDEC VLADFDPAKY NRTRASRGGA NYDSDEEEQG

GEGVQCASQ
Length:409
Mass (Da):44,671
Last modified:May 1, 1992 - v1
Checksum:iE4539F3618DD9CF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56560 Genomic DNA. Translation: CAA39910.1.
S74758 Genomic DNA. Translation: AAB20771.1.
U12141 Genomic DNA. Translation: AAA99647.1.
Z71340 Genomic DNA. Translation: CAA95937.1.
BK006947 Genomic DNA. Translation: DAA10482.1.
PIRiS26703.
RefSeqiNP_014335.1. NM_001182902.1.

Genome annotation databases

EnsemblFungiiYNL064C; YNL064C; YNL064C.
GeneIDi855661.
KEGGisce:YNL064C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56560 Genomic DNA. Translation: CAA39910.1.
S74758 Genomic DNA. Translation: AAB20771.1.
U12141 Genomic DNA. Translation: AAA99647.1.
Z71340 Genomic DNA. Translation: CAA95937.1.
BK006947 Genomic DNA. Translation: DAA10482.1.
PIRiS26703.
RefSeqiNP_014335.1. NM_001182902.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NLTX-ray2.70A103-350[»]
1XAOX-ray2.07A/B258-378[»]
ProteinModelPortaliP25491.
SMRiP25491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35759. 495 interactors.
DIPiDIP-2251N.
IntActiP25491. 16 interactors.
MINTiMINT-603538.

PTM databases

iPTMnetiP25491.

Proteomic databases

MaxQBiP25491.
PRIDEiP25491.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL064C; YNL064C; YNL064C.
GeneIDi855661.
KEGGisce:YNL064C.

Organism-specific databases

EuPathDBiFungiDB:YNL064C.
SGDiS000005008. YDJ1.

Phylogenomic databases

GeneTreeiENSGT00860000133716.
HOGENOMiHOG000226718.
InParanoidiP25491.
KOiK09503.
OMAiIKHEISA.
OrthoDBiEOG092C1UGR.

Enzyme and pathway databases

BioCyciYEAST:G3O-33094-MONOMER.
ReactomeiR-SCE-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiP25491.
PROiP25491.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAS5_YEAST
AccessioniPrimary (citable) accession number: P25491
Secondary accession number(s): D6W1B6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 30, 2016
This is version 171 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 119000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.