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P25491

- MAS5_YEAST

UniProt

P25491 - MAS5_YEAST

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Protein
Mitochondrial protein import protein MAS5
Gene
YDJ1, MAS5, YNL064C, N2418, YNL2418C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Probably involved in mitochondrial protein import. Is also required for efficient translocation of pre-pro-alpha-factor. Involved in heme regulation of HAP1, as a component of the high-molecular-weight (HMC) complex.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161Substrate; via amide nitrogen
Metal bindingi143 – 1431Zinc 1
Metal bindingi146 – 1461Zinc 1
Metal bindingi159 – 1591Zinc 2
Metal bindingi162 – 1621Zinc 2
Metal bindingi185 – 1851Zinc 2
Metal bindingi188 – 1881Zinc 2
Metal bindingi201 – 2011Zinc 1
Metal bindingi204 – 2041Zinc 1
Sitei335 – 3351Involved in dimerization

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri130 – 21384CR-typeUniRule annotation
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. ATPase activator activity Source: SGD
  3. metal ion binding Source: UniProtKB-KW
  4. protein binding Source: IntAct
  5. unfolded protein binding Source: SGD
Complete GO annotation...

GO - Biological processi

  1. 'de novo' protein folding Source: SGD
  2. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  3. positive regulation of ATPase activity Source: GOC
  4. protein refolding Source: SGD
  5. protein targeting to ER Source: SGD
  6. protein targeting to mitochondrion Source: SGD
  7. response to heat Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Stress response, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33094-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial protein import protein MAS5
Alternative name(s):
Yeast dnaJ protein 1
Gene namesi
Name:YDJ1
Synonyms:MAS5
Ordered Locus Names:YNL064C
ORF Names:N2418, YNL2418C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL064c.
SGDiS000005008. YDJ1.

Subcellular locationi

Cytoplasm. Cytoplasmperinuclear region
Note: Concentrated in a perinuclear ring as well as in the cytoplasm.2 Publications

GO - Cellular componenti

  1. TRC complex Source: SGD
  2. cytosol Source: SGD
  3. perinuclear region of cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi335 – 3351F → D: Prevents dimerization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 406406Mitochondrial protein import protein MAS5UniRule annotation
PRO_0000071093Add
BLAST
Propeptidei407 – 4093Removed in mature form Inferred
PRO_0000396684

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei406 – 4061Cysteine methyl ester Inferred
Lipidationi406 – 4061S-farnesyl cysteine1 Publication

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiP25491.
PaxDbiP25491.
PeptideAtlasiP25491.

Expressioni

Inductioni

YDJ1 is a heat shock gene whose expression increases moderately at elevated temperatures.UniRule annotation

Gene expression databases

GenevestigatoriP25491.

Interactioni

Subunit structurei

Homodimer. Interacts with HAP1. Component of the HMC including HAP1, SRO9 and YDJ1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MDY2Q122852EBI-10420,EBI-34904
SGT2Q121182EBI-10420,EBI-31784
SUP35Q7LKB13EBI-10420,EBI-8411471From a different organism.

Protein-protein interaction databases

BioGridi35759. 484 interactions.
DIPiDIP-2251N.
IntActiP25491. 14 interactions.
MINTiMINT-603538.
STRINGi4932.YNL064C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi116 – 1227
Helixi126 – 1294
Beta strandi131 – 14212
Turni144 – 1485
Beta strandi150 – 1523
Turni153 – 1553
Beta strandi162 – 1665
Beta strandi168 – 18215
Beta strandi189 – 1935
Beta strandi202 – 2043
Beta strandi209 – 22012
Beta strandi229 – 2324
Beta strandi247 – 2537
Beta strandi260 – 2623
Beta strandi265 – 2739
Helixi274 – 2796
Beta strandi281 – 2866
Beta strandi288 – 2903
Beta strandi292 – 2976
Turni299 – 3024
Beta strandi308 – 3114
Beta strandi319 – 3213
Beta strandi327 – 3348
Helixi343 – 35210
Beta strandi367 – 3715

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NLTX-ray2.70A103-350[»]
1XAOX-ray2.07A/B258-378[»]
ProteinModelPortaliP25491.
SMRiP25491. Positions 1-71, 110-378.

Miscellaneous databases

EvolutionaryTraceiP25491.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 7269J
Add
BLAST
Repeati143 – 1508CXXCXGXG motifUniRule annotation
Repeati159 – 1668CXXCXGXG motifUniRule annotation
Repeati185 – 1928CXXCXGXG motifUniRule annotation
Repeati201 – 2088CXXCXGXG motifUniRule annotation

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 172172Necessary for HAP1 repression in the absence of hemeUniRule annotation
Add
BLAST
Regioni135 – 1373Substrate bindingUniRule annotation
Regioni215 – 2162Substrate bindingUniRule annotation
Regioni247 – 2493Substrate bindingUniRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi73 – 10331Gly-richUniRule annotation
Add
BLAST

Sequence similaritiesi

Contains 1 J domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0484.
GeneTreeiENSGT00490000043321.
HOGENOMiHOG000226718.
KOiK09503.
OMAiHAGNSAS.
OrthoDBiEOG77M90H.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25491-1 [UniParc]FASTAAdd to Basket

« Hide

MVKETKFYDI LGVPVTATDV EIKKAYRKCA LKYHPDKNPS EEAAEKFKEA    50
SAAYEILSDP EKRDIYDQFG EDGLSGAGGA GGFPGGGFGF GDDIFSQFFG 100
AGGAQRPRGP QRGKDIKHEI SASLEELYKG RTAKLALNKQ ILCKECEGRG 150
GKKGAVKKCT SCNGQGIKFV TRQMGPMIQR FQTECDVCHG TGDIIDPKDR 200
CKSCNGKKVE NERKILEVHV EPGMKDGQRI VFKGEADQAP DVIPGDVVFI 250
VSERPHKSFK RDGDDLVYEA EIDLLTAIAG GEFALEHVSG DWLKVGIVPG 300
EVIAPGMRKV IEGKGMPIPK YGGYGNLIIK FTIKFPENHF TSEENLKKLE 350
EILPPRIVPA IPKKATVDEC VLADFDPAKY NRTRASRGGA NYDSDEEEQG 400
GEGVQCASQ 409
Length:409
Mass (Da):44,671
Last modified:May 1, 1992 - v1
Checksum:iE4539F3618DD9CF2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56560 Genomic DNA. Translation: CAA39910.1.
S74758 Genomic DNA. Translation: AAB20771.1.
U12141 Genomic DNA. Translation: AAA99647.1.
Z71340 Genomic DNA. Translation: CAA95937.1.
BK006947 Genomic DNA. Translation: DAA10482.1.
PIRiS26703.
RefSeqiNP_014335.1. NM_001182902.1.

Genome annotation databases

EnsemblFungiiYNL064C; YNL064C; YNL064C.
GeneIDi855661.
KEGGisce:YNL064C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56560 Genomic DNA. Translation: CAA39910.1 .
S74758 Genomic DNA. Translation: AAB20771.1 .
U12141 Genomic DNA. Translation: AAA99647.1 .
Z71340 Genomic DNA. Translation: CAA95937.1 .
BK006947 Genomic DNA. Translation: DAA10482.1 .
PIRi S26703.
RefSeqi NP_014335.1. NM_001182902.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1NLT X-ray 2.70 A 103-350 [» ]
1XAO X-ray 2.07 A/B 258-378 [» ]
ProteinModelPortali P25491.
SMRi P25491. Positions 1-71, 110-378.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35759. 484 interactions.
DIPi DIP-2251N.
IntActi P25491. 14 interactions.
MINTi MINT-603538.
STRINGi 4932.YNL064C.

Proteomic databases

MaxQBi P25491.
PaxDbi P25491.
PeptideAtlasi P25491.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL064C ; YNL064C ; YNL064C .
GeneIDi 855661.
KEGGi sce:YNL064C.

Organism-specific databases

CYGDi YNL064c.
SGDi S000005008. YDJ1.

Phylogenomic databases

eggNOGi COG0484.
GeneTreei ENSGT00490000043321.
HOGENOMi HOG000226718.
KOi K09503.
OMAi HAGNSAS.
OrthoDBi EOG77M90H.

Enzyme and pathway databases

BioCyci YEAST:G3O-33094-MONOMER.

Miscellaneous databases

EvolutionaryTracei P25491.
NextBioi 979926.
PROi P25491.

Gene expression databases

Genevestigatori P25491.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPi MF_01152. DnaJ.
InterProi IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view ]
Pfami PF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of YDJ1: a yeast homologue of the bacterial dnaJ protein."
    Caplan A.J., Douglas M.G.
    J. Cell Biol. 114:609-621(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
  2. "MAS5, a yeast homolog of DnaJ involved in mitochondrial protein import."
    Atencio D.P., Yaffe M.P.
    Mol. Cell. Biol. 12:283-291(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
    Bergez P., Doignon F., Crouzet M.
    Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Farnesylation of YDJ1p is required for function at elevated growth temperatures in Saccharomyces cerevisiae."
    Caplan A.J., Tsai J., Casey P.J., Douglas M.G.
    J. Biol. Chem. 267:18890-18895(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: ISOPRENYLATION AT CYS-406.
  7. "The Hsp70-Ydj1 molecular chaperone represses the activity of the heme activator protein Hap1 in the absence of heme."
    Hon T., Lee H.C., Hach A., Johnson J.L., Craig E.A., Erdjument-Bromage H., Tempst P., Zhang L.
    Mol. Cell. Biol. 21:7923-7932(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "The crystal structure of the yeast Hsp40 Ydj1 complexed with its peptide substrate."
    Li J., Qian X., Sha B.
    Structure 11:1475-1483(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 103-350 IN COMPLEX WITH SUBSTRATE ANALOGS, SUBUNIT, MUTAGENESIS OF PHE-335.
  13. "The crystal structure of the C-terminal fragment of yeast Hsp40 Ydj1 reveals novel dimerization motif for Hsp40."
    Wu Y., Li J., Jin Z., Fu Z., Sha B.
    J. Mol. Biol. 346:1005-1011(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.07 ANGSTROMS) OF 258-378.

Entry informationi

Entry nameiMAS5_YEAST
AccessioniPrimary (citable) accession number: P25491
Secondary accession number(s): D6W1B6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 11, 2014
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 119000 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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