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P25491

- MAS5_YEAST

UniProt

P25491 - MAS5_YEAST

Protein

Mitochondrial protein import protein MAS5

Gene

YDJ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 148 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Probably involved in mitochondrial protein import. Is also required for efficient translocation of pre-pro-alpha-factor. Involved in heme regulation of HAP1, as a component of the high-molecular-weight (HMC) complex.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei116 – 1161Substrate; via amide nitrogen
    Metal bindingi143 – 1431Zinc 1
    Metal bindingi146 – 1461Zinc 1
    Metal bindingi159 – 1591Zinc 2
    Metal bindingi162 – 1621Zinc 2
    Metal bindingi185 – 1851Zinc 2
    Metal bindingi188 – 1881Zinc 2
    Metal bindingi201 – 2011Zinc 1
    Metal bindingi204 – 2041Zinc 1
    Sitei335 – 3351Involved in dimerization

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri130 – 21384CR-typeAdd
    BLAST

    GO - Molecular functioni

    1. ATPase activator activity Source: SGD
    2. ATP binding Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct
    5. unfolded protein binding Source: SGD

    GO - Biological processi

    1. 'de novo' protein folding Source: SGD
    2. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
    3. positive regulation of ATPase activity Source: GOC
    4. protein refolding Source: SGD
    5. protein targeting to ER Source: SGD
    6. protein targeting to mitochondrion Source: SGD
    7. response to heat Source: InterPro

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Protein transport, Stress response, Transport

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33094-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial protein import protein MAS5
    Alternative name(s):
    Yeast dnaJ protein 1
    Gene namesi
    Name:YDJ1
    Synonyms:MAS5
    Ordered Locus Names:YNL064C
    ORF Names:N2418, YNL2418C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL064c.
    SGDiS000005008. YDJ1.

    Subcellular locationi

    Cytoplasm. Cytoplasmperinuclear region
    Note: Concentrated in a perinuclear ring as well as in the cytoplasm.

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. perinuclear region of cytoplasm Source: SGD
    3. TRC complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi335 – 3351F → D: Prevents dimerization. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 406406Mitochondrial protein import protein MAS5PRO_0000071093Add
    BLAST
    Propeptidei407 – 4093Removed in mature formCuratedPRO_0000396684

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei406 – 4061Cysteine methyl esterCurated
    Lipidationi406 – 4061S-farnesyl cysteine1 Publication

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    MaxQBiP25491.
    PaxDbiP25491.
    PeptideAtlasiP25491.

    Expressioni

    Inductioni

    YDJ1 is a heat shock gene whose expression increases moderately at elevated temperatures.

    Gene expression databases

    GenevestigatoriP25491.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with HAP1. Component of the HMC including HAP1, SRO9 and YDJ1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MDY2Q122852EBI-10420,EBI-34904
    SGT2Q121182EBI-10420,EBI-31784
    SUP35Q7LKB13EBI-10420,EBI-8411471From a different organism.

    Protein-protein interaction databases

    BioGridi35759. 484 interactions.
    DIPiDIP-2251N.
    IntActiP25491. 14 interactions.
    MINTiMINT-603538.
    STRINGi4932.YNL064C.

    Structurei

    Secondary structure

    1
    409
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi116 – 1227
    Helixi126 – 1294
    Beta strandi131 – 14212
    Turni144 – 1485
    Beta strandi150 – 1523
    Turni153 – 1553
    Beta strandi162 – 1665
    Beta strandi168 – 18215
    Beta strandi189 – 1935
    Beta strandi202 – 2043
    Beta strandi209 – 22012
    Beta strandi229 – 2324
    Beta strandi247 – 2537
    Beta strandi260 – 2623
    Beta strandi265 – 2739
    Helixi274 – 2796
    Beta strandi281 – 2866
    Beta strandi288 – 2903
    Beta strandi292 – 2976
    Turni299 – 3024
    Beta strandi308 – 3114
    Beta strandi319 – 3213
    Beta strandi327 – 3348
    Helixi343 – 35210
    Beta strandi367 – 3715

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NLTX-ray2.70A103-350[»]
    1XAOX-ray2.07A/B258-378[»]
    ProteinModelPortaliP25491.
    SMRiP25491. Positions 1-71, 110-378.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP25491.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 7269JAdd
    BLAST
    Repeati143 – 1508CXXCXGXG motif
    Repeati159 – 1668CXXCXGXG motif
    Repeati185 – 1928CXXCXGXG motif
    Repeati201 – 2088CXXCXGXG motif

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 172172Necessary for HAP1 repression in the absence of hemeAdd
    BLAST
    Regioni135 – 1373Substrate binding
    Regioni215 – 2162Substrate binding
    Regioni247 – 2493Substrate binding

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi73 – 10331Gly-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CR-type zinc finger.Curated
    Contains 1 J domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri130 – 21384CR-typeAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0484.
    GeneTreeiENSGT00490000043321.
    HOGENOMiHOG000226718.
    KOiK09503.
    OMAiHAGNSAS.
    OrthoDBiEOG77M90H.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPiMF_01152. DnaJ.
    InterProiIPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    [Graphical view]
    PfamiPF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view]
    PRINTSiPR00625. JDOMAIN.
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    PROSITEiPS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25491-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKETKFYDI LGVPVTATDV EIKKAYRKCA LKYHPDKNPS EEAAEKFKEA    50
    SAAYEILSDP EKRDIYDQFG EDGLSGAGGA GGFPGGGFGF GDDIFSQFFG 100
    AGGAQRPRGP QRGKDIKHEI SASLEELYKG RTAKLALNKQ ILCKECEGRG 150
    GKKGAVKKCT SCNGQGIKFV TRQMGPMIQR FQTECDVCHG TGDIIDPKDR 200
    CKSCNGKKVE NERKILEVHV EPGMKDGQRI VFKGEADQAP DVIPGDVVFI 250
    VSERPHKSFK RDGDDLVYEA EIDLLTAIAG GEFALEHVSG DWLKVGIVPG 300
    EVIAPGMRKV IEGKGMPIPK YGGYGNLIIK FTIKFPENHF TSEENLKKLE 350
    EILPPRIVPA IPKKATVDEC VLADFDPAKY NRTRASRGGA NYDSDEEEQG 400
    GEGVQCASQ 409
    Length:409
    Mass (Da):44,671
    Last modified:May 1, 1992 - v1
    Checksum:iE4539F3618DD9CF2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56560 Genomic DNA. Translation: CAA39910.1.
    S74758 Genomic DNA. Translation: AAB20771.1.
    U12141 Genomic DNA. Translation: AAA99647.1.
    Z71340 Genomic DNA. Translation: CAA95937.1.
    BK006947 Genomic DNA. Translation: DAA10482.1.
    PIRiS26703.
    RefSeqiNP_014335.1. NM_001182902.1.

    Genome annotation databases

    EnsemblFungiiYNL064C; YNL064C; YNL064C.
    GeneIDi855661.
    KEGGisce:YNL064C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56560 Genomic DNA. Translation: CAA39910.1 .
    S74758 Genomic DNA. Translation: AAB20771.1 .
    U12141 Genomic DNA. Translation: AAA99647.1 .
    Z71340 Genomic DNA. Translation: CAA95937.1 .
    BK006947 Genomic DNA. Translation: DAA10482.1 .
    PIRi S26703.
    RefSeqi NP_014335.1. NM_001182902.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1NLT X-ray 2.70 A 103-350 [» ]
    1XAO X-ray 2.07 A/B 258-378 [» ]
    ProteinModelPortali P25491.
    SMRi P25491. Positions 1-71, 110-378.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35759. 484 interactions.
    DIPi DIP-2251N.
    IntActi P25491. 14 interactions.
    MINTi MINT-603538.
    STRINGi 4932.YNL064C.

    Proteomic databases

    MaxQBi P25491.
    PaxDbi P25491.
    PeptideAtlasi P25491.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL064C ; YNL064C ; YNL064C .
    GeneIDi 855661.
    KEGGi sce:YNL064C.

    Organism-specific databases

    CYGDi YNL064c.
    SGDi S000005008. YDJ1.

    Phylogenomic databases

    eggNOGi COG0484.
    GeneTreei ENSGT00490000043321.
    HOGENOMi HOG000226718.
    KOi K09503.
    OMAi HAGNSAS.
    OrthoDBi EOG77M90H.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33094-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P25491.
    NextBioi 979926.
    PROi P25491.

    Gene expression databases

    Genevestigatori P25491.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPi MF_01152. DnaJ.
    InterProi IPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    [Graphical view ]
    Pfami PF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view ]
    PRINTSi PR00625. JDOMAIN.
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    PROSITEi PS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of YDJ1: a yeast homologue of the bacterial dnaJ protein."
      Caplan A.J., Douglas M.G.
      J. Cell Biol. 114:609-621(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
    2. "MAS5, a yeast homolog of DnaJ involved in mitochondrial protein import."
      Atencio D.P., Yaffe M.P.
      Mol. Cell. Biol. 12:283-291(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
      Bergez P., Doignon F., Crouzet M.
      Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Farnesylation of YDJ1p is required for function at elevated growth temperatures in Saccharomyces cerevisiae."
      Caplan A.J., Tsai J., Casey P.J., Douglas M.G.
      J. Biol. Chem. 267:18890-18895(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: ISOPRENYLATION AT CYS-406.
    7. "The Hsp70-Ydj1 molecular chaperone represses the activity of the heme activator protein Hap1 in the absence of heme."
      Hon T., Lee H.C., Hach A., Johnson J.L., Craig E.A., Erdjument-Bromage H., Tempst P., Zhang L.
      Mol. Cell. Biol. 21:7923-7932(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "The crystal structure of the yeast Hsp40 Ydj1 complexed with its peptide substrate."
      Li J., Qian X., Sha B.
      Structure 11:1475-1483(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 103-350 IN COMPLEX WITH SUBSTRATE ANALOGS, SUBUNIT, MUTAGENESIS OF PHE-335.
    13. "The crystal structure of the C-terminal fragment of yeast Hsp40 Ydj1 reveals novel dimerization motif for Hsp40."
      Wu Y., Li J., Jin Z., Fu Z., Sha B.
      J. Mol. Biol. 346:1005-1011(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.07 ANGSTROMS) OF 258-378.

    Entry informationi

    Entry nameiMAS5_YEAST
    AccessioniPrimary (citable) accession number: P25491
    Secondary accession number(s): D6W1B6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 148 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 119000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3