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P25472

- GUND_CLOCE

UniProt

P25472 - GUND_CLOCE

Protein

Endoglucanase D

Gene

celCCD

Organism
Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei159 – 1591Proton donorBy similarity
    Active sitei264 – 2641NucleophileBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciCCEL394503:GJET-865-MONOMER.
    UniPathwayiUPA00696.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase D (EC:3.2.1.4)
    Alternative name(s):
    Cellulase D
    EGCCD
    Endo-1,4-beta-glucanase D
    Gene namesi
    Name:celCCD
    Ordered Locus Names:Ccel_0840
    OrganismiClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
    Taxonomic identifieri394503 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001349: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 584560Endoglucanase DPRO_0000007846Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi394503.Ccel_0840.

    Structurei

    3D structure databases

    ProteinModelPortaliP25472.
    SMRiP25472. Positions 354-523.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini354 – 584231CBM11Add
    BLAST
    Repeati530 – 552231Add
    BLAST
    Repeati562 – 584232Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni25 – 328304CatalyticBy similarityAdd
    BLAST
    Regioni530 – 584552 X 24 AA approximate repeatsAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi329 – 35325Pro/Thr-rich (linker)Add
    BLAST

    Domaini

    A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG2730.
    KOiK01179.
    OMAiDATASCK.
    OrthoDBiEOG66F040.

    Family and domain databases

    Gene3Di1.10.1330.10. 1 hit.
    2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR005087. CBM_fam11.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR018242. Dockerin_1.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR008979. Galactose-bd-like.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF03425. CBM_11. 1 hit.
    PF00150. Cellulase. 1 hit.
    PF00404. Dockerin_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25472-1 [UniParc]FASTAAdd to Basket

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    MKKILALIIS CSIIMSFLPM SVYGAINSQD MVKKMGIGMN LGNTFDAPTE    50
    GSWSKAAQEY YFDDFKQAGF KHVRIPIRWD QHTLANSPYT VDSNFLNRIE 100
    TVIDWSLSRG FVTVINSHHD TWLMDNYSQN IGRFEKIWEQ IAQRFKGKSE 150
    NLVFEILNEP HGNITDSQIN DMNKRILNII RKTNPTRNVI IGAGYWNSYN 200
    SLSQLEIPND PNLIATFHYY DPYSFTHQWQ GTWGTKNDMD AIAMVFNHVK 250
    KWSDKNNIPV YLGEYGVMGH SDRTSAVKWF DFVSDQAISH GFSCGAWDNG 300
    VFGSVDNDMA FYNRDTRQFD KEILNAILTT GTTYDWTPPT ETNPDPPRTP 350
    ATPAYGEQLI EDFEGAMQWA AYSGVDATAS CKISSGKSNN GLEITYAGSS 400
    NGYWGVVDNE HRNQDWEKWQ KISFDIKSSN TNEVRLLIAE QSKIEGEDGE 450
    HWTYVIKPST SWTTIEIPFS SFTKRMDYQP PAQDGSETFD LYKVGSLHFM 500
    YSNSNSGTLN IDNIKLIGLP EEQIGGKIGD VNEDGNIDAI DFALLKKYLL 550
    DSSISINKVN ADINLDGDIN AIDFAKLKMM LLGD 584
    Length:584
    Mass (Da):66,062
    Last modified:May 1, 1992 - v1
    Checksum:i0FC41257E81322C3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90341 Genomic DNA. Translation: BAA14354.1.
    CP001348 Genomic DNA. Translation: ACL75216.1.
    RefSeqiWP_015924376.1. NC_011898.1.
    YP_002505196.1. NC_011898.1.

    Genome annotation databases

    EnsemblBacteriaiACL75216; ACL75216; Ccel_0840.
    GeneIDi7309687.
    KEGGicce:Ccel_0840.
    PATRICi19432493. VBICloCel57783_0870.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90341 Genomic DNA. Translation: BAA14354.1 .
    CP001348 Genomic DNA. Translation: ACL75216.1 .
    RefSeqi WP_015924376.1. NC_011898.1.
    YP_002505196.1. NC_011898.1.

    3D structure databases

    ProteinModelPortali P25472.
    SMRi P25472. Positions 354-523.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 394503.Ccel_0840.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL75216 ; ACL75216 ; Ccel_0840 .
    GeneIDi 7309687.
    KEGGi cce:Ccel_0840.
    PATRICi 19432493. VBICloCel57783_0870.

    Phylogenomic databases

    eggNOGi COG2730.
    KOi K01179.
    OMAi DATASCK.
    OrthoDBi EOG66F040.

    Enzyme and pathway databases

    UniPathwayi UPA00696 .
    BioCyci CCEL394503:GJET-865-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1330.10. 1 hit.
    2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR005087. CBM_fam11.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR018242. Dockerin_1.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR008979. Galactose-bd-like.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF03425. CBM_11. 1 hit.
    PF00150. Cellulase. 1 hit.
    PF00404. Dockerin_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence analysis of the endoglucanase-encoding gene, celCCD, of Clostridium cellulolyticum."
      Shima S., Igarashi Y., Kodama T.
      Gene 104:33-38(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35319 / DSM 5812 / JCM 6584 / H10.

    Entry informationi

    Entry nameiGUND_CLOCE
    AccessioniPrimary (citable) accession number: P25472
    Secondary accession number(s): B8I8I3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3