Reviewed, UniProtKB/Swiss-Prot P25466 (HN_PI2HT)

Names and origin

Protein names

Recommended name:
    Hemagglutinin-neuraminidase
    EC=3.2.1.18

Gene names

Name:

HN

Organism

Human parainfluenza 2 virus (strain Toshiba) (HPIV-2)

Taxonomic identifier

11214 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Rubulavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Ontologies

Keywords
Cellular component	Cell membrane Envelope protein Membrane Virion
Domain	Signal-anchor Transmembrane
Molecular function	Hemagglutinin Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: EC host cell surface receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Hemagglutinin-neuraminidase		PRO_0000142623
Regions
	Topological domain	1 – 25	25	Cytoplasmic Potential
	Transmembrane	26 – 46	21	Potential
	Topological domain	47 – 571	525	Extracellular Potential
	Region	228 – 233	6	Important for neuraminidase activity By similarity
	Region	393 – 398	6	Sialic receptor-binding site Probable
Amino acid modifications
	Glycosylation	272	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	284	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	335	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	341	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	386	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	454	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	498	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	501	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	517	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	522	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	P25466-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: D7130A3BE6491406	FASTA	571	63,263
	10 20 30 40 50 60 MEDYSNLSLK SIPKRTCRII FRTATILGIC TLIVLCSSIL HEIIHLDVSS GLMDSDDSQQ 70 80 90 100 110 120 GIIQPIIESL KSLIALANQI LYNVAIIIPL KIDSIETVIF SALKDMHTGS MSNTNCTPGN 130 140 150 160 170 180 LLLHDAAYIN GINKFLVLKS YNGTPKYGPL LNIPSFIPSA TSPNGCTRIP SFSLIKTHWC 190 200 210 220 230 240 YTHNVMLGDC LDFTTSNQYL AMGIIQQSAA AFPIFRTMKT IYLSDGINRK SCSVTAIPGG 250 260 270 280 290 300 CVLYCYVATR SEKEDYATTD LAELRLAFYY YNDTFIERVI SLPNTTGQWA TINPAVGSGI 310 320 330 340 350 360 YHLGFILFPV YGGLISGTPS YNKQSSRYFI PKHPNITCAG NSSEQAAAAR SSYVIRYHSN 370 380 390 400 410 420 RLIQSAVLIC PLSDMHTARC NLVMFNNSQV MMGAEGRLYV IDNNLYYYQR SSSWWSASLF 430 440 450 460 470 480 YRINTDFSKG IPPIIEAQWV PSYQVPRPGV MPCNATSFCP ANCITGVYAD VWPLNDPEPT 490 500 510 520 530 540 SQNALNPNYR FAGAFLRNES NRTNPTFYTA SASALLNTTG FNNTNHKAAY TSSTCFKNTG 550 560 570 TQKIYCLIII EMGSSLLGEF QIIPFLRELI P

References

[1]

"Sequence determination of the hemagglutinin-neuraminidase (HN) gene of human parainfluenza type 2 virus and the construction of a phylogenetic tree for HN proteins of all the paramyxoviruses that are infectious to humans." Kawano M., Bando H., Yuasa T., Kondo K., Tsurudome M., Komada H., Nishio M., Ito Y. Virology 174:308-313(1990) [PubMed: 2152995] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X57559 mRNA. Translation: CAA40787.1.
PIR	HNNZP2. A33777.
RefSeq	NP_598405.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH83. Glycoside Hydrolase Family 83.
Genome annotation databases
GeneID	935188.
Family and domain databases
InterPro	IPR000665. Hemagglutn-neuramid_glycoprot. IPR016285. Hemagglutn-neuramid_paramyxo. [Graphical view]
Pfam	PF00423. HN. 1 hit. [Graphical view]
PIRSF	PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HN_PI2HT

Accession

Primary (citable) accession number: P25466

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)