P25465 (HN_PI2H) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hemagglutinin-neuraminidase
EC=3.2.1.18

Gene names

Name:

HN

Organism

Human parainfluenza 2 virus (HPIV-2)

Taxonomic identifier

11212 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Rubulavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Ontologies

Keywords
Biological process	Host-virus interaction Viral attachment to host cell Virus entry into host cell
Cellular component	Host cell membrane Host membrane Membrane Viral envelope protein Virion
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hemagglutinin Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	exo-alpha-(2->3)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->6)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->8)-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Hemagglutinin-neuraminidase		PRO_0000142622
Regions
	Topological domain	1 – 25	25	Intravirion Potential
	Transmembrane	26 – 46	21	Helical; Potential
	Topological domain	47 – 571	525	Virion surface Potential
Amino acid modifications
	Glycosylation	272	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	284	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	335	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	386	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	454	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	498	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	501	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	517	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	522	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	P25465 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 56B4DCFA4337F392	FASTA	571	63,316
	10 20 30 40 50 60 MEDYSNLSLK SIPKRTCRII FRTATILGIC TLIVLCSSIL HEIIHLDVSS GLMDSDDSQQ 70 80 90 100 110 120 GIIQPIIESL KSLIALANQI LYNVAIIIPL KIDSIETVIY SALKDMHTGS MSNTNCTPGN 130 140 150 160 170 180 LLLHDAAYIN GLNKFLVLKS YNGTPKYGPL LNIPSFIPSA TSPNGCTRIP SFSLIKTHWC 190 200 210 220 230 240 YTHNVILGDC LDFTTSNQYL AMGIIQQSAA AFPIFRTMKT IYLSDGINRK SCSVTAIPGG 250 260 270 280 290 300 CVLYCYVATR SEKEDYATTD LAELRLAFYY YNDTFIERVI SLPNTTGQWA TINPAVGSGI 310 320 330 340 350 360 YHLGFILFPV YGGLIKGTPS YNKQSSRYFI PKHPNITCAG KSSEQAAAAR SSYVIRYHSN 370 380 390 400 410 420 RLLQSAVLIC PLSDMHTARC NLVMFNNSQV MMGAEGRLYV IDNNLYYYQR SSSWWSASLF 430 440 450 460 470 480 YRINTDFSKG IPPIIEAQWV PSYQVPRPGV MPCNATSFCP ANCITGVYAD VWPLNDPEPT 490 500 510 520 530 540 SQNALNPNYR FAGAFLRNES NRTNPTFYTA SASALLNTTG FNNTNHKAAY TSSTCFKNTG 550 560 570 TQKIYCLIII EMGSSLLGEF QIIPFLRELI P

References

[1]	"Sequence analysis of the HN gene of parainfluenza virus type 2." Precious B., Southern J.A., Randall R.E. J. Gen. Virol. 71:1163-1168(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00865 mRNA. Translation: BAA00739.1.
PIR	HNNZT2. A34767.
3D structure databases
ProteinModelPortal	P25465.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.120.10.10. 1 hit.
InterPro	IPR000665. Hemagglutn-neuramid. IPR016285. Hemagglutn-neuramid_paramyxo. IPR011040. Sialidases. [Graphical view]
Pfam	PF00423. HN. 1 hit. [Graphical view]
PIRSF	PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAM	SSF50939. Sialidase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HN_PI2H

Accession

Primary (citable) accession number: P25465

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program