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P25462 (GLNAC_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase, chloroplastic

EC=6.3.1.2
Alternative name(s):
GS2
Glutamate--ammonia ligase
Gene names
Name:GLN2
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer.

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

response to cadmium ion

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to salt stress

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentapoplast

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast envelope

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast stroma

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast thylakoid membrane

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

cytosolic ribosome

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5151Chloroplast Potential
Chain52 – 423372Glutamine synthetase, chloroplastic
PRO_0000011179

Sequences

Sequence LengthMass (Da)Tools
P25462 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: EC799D71E0BD6C5F

FASTA42346,017
        10         20         30         40         50         60 
MAQAVVPAMQ CRVGVKAAAG RVWSAGRTRT GRGGASPGFK VMAVSTGSTG VVPRLEQLLN 

        70         80         90        100        110        120 
MDTTPYTDKV IAEYIWVGGS GIDIRSKSRT ISKPVEDPSE LPKWNYDGSS TGQAPGEDSE 

       130        140        150        160        170        180 
VILYPQAIFK DPFRGGNNVL VICDTYTPQG EPLPTNKRHR AAQIFSDPKV GEQVPWFGIE 

       190        200        210        220        230        240 
QEYTLLQKDV NWPLGWPVGG FPGPQGPYYC AVGADKSFGR DISDAHYKAC LYAGINISGT 

       250        260        270        280        290        300 
NGEVMPGQWE YQVGPSVGIE AGDHIWISRY ILERITEQAG VVLTLDPKPI QGDWNGAGCH 

       310        320        330        340        350        360 
TNYSTKTMRE DGGFEEIKRA ILNLSLRHDL HISAYGEGNE RRLTGKHETA SIGTFSWGVA 

       370        380        390        400        410        420 
NRGCSIRVGR DTEAKGKGYL EDRRPASNMD PYIVTGLLAE TTILWQPSLE AEALAAKKLA 


LKV 

« Hide

References

[1]"Maize glutamine synthetase cDNAs: isolation by direct genetic selection in Escherichia coli."
Snustad D.P., Hunsperger J.P., Chereskin B.M., Messing J.
Genetics 120:1111-1124(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Black Mexican Sweet.
Tissue: Leaf.
[2]Patthey J.-P.
Unpublished observations (MAY-1992)
Cited for: POSSIBLE FRAMESHIFT ERROR IN SEQUENCE DESCRIBED IN PUBMED:2906306.
[3]"Differential expression of six glutamine synthetase genes in Zea mays."
Li M.-G., Villemur R., Hussey P.J., Silflow C.D., Gantt J.S., Snustad D.P.
Plant Mol. Biol. 23:401-407(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Black Mexican Sweet.
Tissue: Seedling.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X65931 mRNA. Translation: CAA46724.1.
PIRS39482.
RefSeqNP_001105725.1. NM_001112255.1.
UniGeneZm.160909.

3D structure databases

ProteinModelPortalP25462.
SMRP25462. Positions 55-406.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP25462.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID542746.
KEGGzma:542746.

Organism-specific databases

GrameneP25462.
MaizeGDB61728.

Phylogenomic databases

HOGENOMHOG000061500.
KOK01915.

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNAC_MAIZE
AccessionPrimary (citable) accession number: P25462
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: March 19, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families