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Protein

Glutamine synthetase, chloroplastic

Gene

GLN2

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. aging Source: EnsemblPlants/Gramene
  2. ammonia assimilation cycle Source: EnsemblPlants/Gramene
  3. cysteine biosynthetic process Source: EnsemblPlants/Gramene
  4. glutamine biosynthetic process Source: EnsemblPlants/Gramene
  5. glycolytic process Source: EnsemblPlants/Gramene
  6. Golgi organization Source: EnsemblPlants/Gramene
  7. hyperosmotic response Source: EnsemblPlants/Gramene
  8. pentose-phosphate shunt Source: EnsemblPlants/Gramene
  9. regulation of proton transport Source: EnsemblPlants/Gramene
  10. response to blue light Source: EnsemblPlants/Gramene
  11. response to cold Source: EnsemblPlants/Gramene
  12. response to far red light Source: EnsemblPlants/Gramene
  13. response to fructose Source: EnsemblPlants/Gramene
  14. response to glucose Source: EnsemblPlants/Gramene
  15. response to high light intensity Source: EnsemblPlants/Gramene
  16. response to red light Source: EnsemblPlants/Gramene
  17. response to sucrose Source: EnsemblPlants/Gramene
  18. water transport Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 6752.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase, chloroplastic (EC:6.3.1.2)
Alternative name(s):
GS2
Glutamate--ammonia ligase
Gene namesi
Name:GLN2
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea
ProteomesiUP000007305 Componenti: Unplaced

Organism-specific databases

GrameneiP25462.
MaizeGDBi61728.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: EnsemblPlants/Gramene
  2. chloroplast envelope Source: EnsemblPlants/Gramene
  3. chloroplast stroma Source: EnsemblPlants/Gramene
  4. chloroplast thylakoid membrane Source: EnsemblPlants/Gramene
  5. cytosolic ribosome Source: EnsemblPlants/Gramene
  6. mitochondrion Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5151ChloroplastSequence AnalysisAdd
BLAST
Chaini52 – 423372Glutamine synthetase, chloroplasticPRO_0000011179Add
BLAST

Proteomic databases

PRIDEiP25462.

Expressioni

Gene expression databases

ExpressionAtlasiP25462. baseline and differential.

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliP25462.
SMRiP25462. Positions 55-406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000061500.
KOiK01915.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQAVVPAMQ CRVGVKAAAG RVWSAGRTRT GRGGASPGFK VMAVSTGSTG
60 70 80 90 100
VVPRLEQLLN MDTTPYTDKV IAEYIWVGGS GIDIRSKSRT ISKPVEDPSE
110 120 130 140 150
LPKWNYDGSS TGQAPGEDSE VILYPQAIFK DPFRGGNNVL VICDTYTPQG
160 170 180 190 200
EPLPTNKRHR AAQIFSDPKV GEQVPWFGIE QEYTLLQKDV NWPLGWPVGG
210 220 230 240 250
FPGPQGPYYC AVGADKSFGR DISDAHYKAC LYAGINISGT NGEVMPGQWE
260 270 280 290 300
YQVGPSVGIE AGDHIWISRY ILERITEQAG VVLTLDPKPI QGDWNGAGCH
310 320 330 340 350
TNYSTKTMRE DGGFEEIKRA ILNLSLRHDL HISAYGEGNE RRLTGKHETA
360 370 380 390 400
SIGTFSWGVA NRGCSIRVGR DTEAKGKGYL EDRRPASNMD PYIVTGLLAE
410 420
TTILWQPSLE AEALAAKKLA LKV
Length:423
Mass (Da):46,017
Last modified:April 30, 1992 - v1
Checksum:iEC799D71E0BD6C5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65931 mRNA. Translation: CAA46724.1.
PIRiS39482.
RefSeqiNP_001105725.1. NM_001112255.1.
UniGeneiZm.160909.
Zm.3369.

Genome annotation databases

GeneIDi542746.
KEGGizma:542746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65931 mRNA. Translation: CAA46724.1.
PIRiS39482.
RefSeqiNP_001105725.1. NM_001112255.1.
UniGeneiZm.160909.
Zm.3369.

3D structure databases

ProteinModelPortaliP25462.
SMRiP25462. Positions 55-406.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL2285357.

Proteomic databases

PRIDEiP25462.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi542746.
KEGGizma:542746.

Organism-specific databases

GrameneiP25462.
MaizeGDBi61728.

Phylogenomic databases

HOGENOMiHOG000061500.
KOiK01915.

Enzyme and pathway databases

BRENDAi6.3.1.2. 6752.

Gene expression databases

ExpressionAtlasiP25462. baseline and differential.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Maize glutamine synthetase cDNAs: isolation by direct genetic selection in Escherichia coli."
    Snustad D.P., Hunsperger J.P., Chereskin B.M., Messing J.
    Genetics 120:1111-1124(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Black Mexican Sweet.
    Tissue: Leaf.
  2. Patthey J.-P.
    Unpublished observations (APR-1992)
    Cited for: POSSIBLE FRAMESHIFT ERROR IN SEQUENCE DESCRIBED IN PUBMED:2906306.
  3. "Differential expression of six glutamine synthetase genes in Zea mays."
    Li M.-G., Villemur R., Hussey P.J., Silflow C.D., Gantt J.S., Snustad D.P.
    Plant Mol. Biol. 23:401-407(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Black Mexican Sweet.
    Tissue: Seedling.

Entry informationi

Entry nameiGLNAC_MAIZE
AccessioniPrimary (citable) accession number: P25462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 1992
Last sequence update: April 30, 1992
Last modified: March 31, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.