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P25462

- GLNAC_MAIZE

UniProt

P25462 - GLNAC_MAIZE

Protein

Glutamine synthetase, chloroplastic

Gene

GLN2

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. response to cadmium ion Source: EnsemblPlants/Gramene
    3. response to salt stress Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase, chloroplastic (EC:6.3.1.2)
    Alternative name(s):
    GS2
    Glutamate--ammonia ligase
    Gene namesi
    Name:GLN2
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiP25462.
    MaizeGDBi61728.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: EnsemblPlants/Gramene
    2. chloroplast envelope Source: EnsemblPlants/Gramene
    3. chloroplast stroma Source: EnsemblPlants/Gramene
    4. chloroplast thylakoid membrane Source: EnsemblPlants/Gramene
    5. cytosolic ribosome Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5151ChloroplastSequence AnalysisAdd
    BLAST
    Chaini52 – 423372Glutamine synthetase, chloroplasticPRO_0000011179Add
    BLAST

    Proteomic databases

    PRIDEiP25462.

    Interactioni

    Subunit structurei

    Homooctamer.

    Structurei

    3D structure databases

    ProteinModelPortaliP25462.
    SMRiP25462. Positions 55-406.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000061500.
    KOiK01915.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25462-1 [UniParc]FASTAAdd to Basket

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    MAQAVVPAMQ CRVGVKAAAG RVWSAGRTRT GRGGASPGFK VMAVSTGSTG    50
    VVPRLEQLLN MDTTPYTDKV IAEYIWVGGS GIDIRSKSRT ISKPVEDPSE 100
    LPKWNYDGSS TGQAPGEDSE VILYPQAIFK DPFRGGNNVL VICDTYTPQG 150
    EPLPTNKRHR AAQIFSDPKV GEQVPWFGIE QEYTLLQKDV NWPLGWPVGG 200
    FPGPQGPYYC AVGADKSFGR DISDAHYKAC LYAGINISGT NGEVMPGQWE 250
    YQVGPSVGIE AGDHIWISRY ILERITEQAG VVLTLDPKPI QGDWNGAGCH 300
    TNYSTKTMRE DGGFEEIKRA ILNLSLRHDL HISAYGEGNE RRLTGKHETA 350
    SIGTFSWGVA NRGCSIRVGR DTEAKGKGYL EDRRPASNMD PYIVTGLLAE 400
    TTILWQPSLE AEALAAKKLA LKV 423
    Length:423
    Mass (Da):46,017
    Last modified:May 1, 1992 - v1
    Checksum:iEC799D71E0BD6C5F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65931 mRNA. Translation: CAA46724.1.
    PIRiS39482.
    RefSeqiNP_001105725.1. NM_001112255.1.
    UniGeneiZm.160909.

    Genome annotation databases

    GeneIDi542746.
    KEGGizma:542746.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65931 mRNA. Translation: CAA46724.1 .
    PIRi S39482.
    RefSeqi NP_001105725.1. NM_001112255.1.
    UniGenei Zm.160909.

    3D structure databases

    ProteinModelPortali P25462.
    SMRi P25462. Positions 55-406.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P25462.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 542746.
    KEGGi zma:542746.

    Organism-specific databases

    Gramenei P25462.
    MaizeGDBi 61728.

    Phylogenomic databases

    HOGENOMi HOG000061500.
    KOi K01915.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Maize glutamine synthetase cDNAs: isolation by direct genetic selection in Escherichia coli."
      Snustad D.P., Hunsperger J.P., Chereskin B.M., Messing J.
      Genetics 120:1111-1124(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Black Mexican Sweet.
      Tissue: Leaf.
    2. Patthey J.-P.
      Unpublished observations (MAY-1992)
      Cited for: POSSIBLE FRAMESHIFT ERROR IN SEQUENCE DESCRIBED IN PUBMED:2906306.
    3. "Differential expression of six glutamine synthetase genes in Zea mays."
      Li M.-G., Villemur R., Hussey P.J., Silflow C.D., Gantt J.S., Snustad D.P.
      Plant Mol. Biol. 23:401-407(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Black Mexican Sweet.
      Tissue: Seedling.

    Entry informationi

    Entry nameiGLNAC_MAIZE
    AccessioniPrimary (citable) accession number: P25462
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3