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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase classes I and II

Gene

Plc21C

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei333 – 3331PROSITE-ProRule annotation
Active sitei378 – 3781PROSITE-ProRule annotation
Binding sitei464 – 4641SubstrateBy similarity
Binding sitei466 – 4661SubstrateBy similarity
Binding sitei628 – 6281SubstrateBy similarity
Binding sitei655 – 6551SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

  • flight behavior Source: FlyBase
  • intracellular signal transduction Source: InterPro
  • lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiREACT_282109. G alpha (q) signalling events.
REACT_288405. Synthesis of IP3 and IP4 in the cytosol.
REACT_349107. PLC beta mediated events.
REACT_352779. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
REACT_353102. Acetylcholine regulates insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase classes I and II (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C
Gene namesi
Name:Plc21C
Synonyms:plc-21
ORF Names:CG4574
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0004611. Plc21C.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 131813181-phosphatidylinositol 4,5-bisphosphate phosphodiesterase classes I and IIPRO_0000088511Add
BLAST

Proteomic databases

PaxDbiP25455.

Expressioni

Tissue specificityi

Expressed in neuronal cell bodies of the optic lobe, central brain, and thoracic ganglia in adults, and the brain of larvae.1 Publication

Gene expression databases

BgeeiP25455.
GenevisibleiP25455. DM.

Interactioni

Protein-protein interaction databases

BioGridi59460. 1 interaction.
IntActiP25455. 4 interactions.
STRINGi7227.FBpp0089226.

Structurei

3D structure databases

ProteinModelPortaliP25455.
SMRiP25455. Positions 16-468, 568-892.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini318 – 466149PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini599 – 715117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini722 – 82099C2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi469 – 47810His-rich

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00760000118936.
InParanoidiP25455.
KOiK05858.
OMAiDANTTQF.
OrthoDBiEOG7WDN1N.
PhylomeDBiP25455.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028407. PLC21C.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF87. PTHR10336:SF87. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform D (identifier: P25455-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMSAGGTYIS TASVEVPQAL QDGEKFIRWD DDSGTGTPVT MRVDAKGFFL
60 70 80 90 100
YWVDQNNELD ILDIATIRDV RTGQYAKRPK DNKLRQIVTL GPQDTLEEKT
110 120 130 140 150
VTVCHGSDFV NMTFVNFCCT RRDIAQLWTD GLIKLAYSLA QLNGSAIMFL
160 170 180 190 200
QKAHTKLCLQ VDKSGRIPVK NIIKLFAQNK EDRKRVEKAL DVTGLPSGKV
210 220 230 240 250
DSISVSKFQF EDFYNLYKYL TQRSEVERLF DSIVGNSKRK CMSIAQLVEF
260 270 280 290 300
LNKTQRDPRL NEILYPYANP ARAKELIQQY EPNKFNAQKG QLSLDGFLRY
310 320 330 340 350
LMGDDNPIMA PSKLDLCDDM DQPMSHYFIN SSHNTYLTGH QLTGKSSVEI
360 370 380 390 400
YRQCLLAGCR CVELDFWNGR TEEPVIVHGY TFVPEIFAKD VLEAIAESAF
410 420 430 440 450
KTSEYPVILS FENHCNPRQQ AKIANYCREI FGDMLLDKPL DSHPLEPNMD
460 470 480 490 500
LPPPAMLRRK IIIKNKKKHH HHHHHHHHKK PAQVGTPAAN NKLTTANSVD
510 520 530 540 550
AKAAQQVGLS ASHEDGGVTR STANGDVATG TGTGSAAGTA GHAPPLQQIR
560 570 580 590 600
QSSKDSTGSS DSDSSSEDES LPNTTPNLPS GNEPPPEKAQ KETEAGAEIS
610 620 630 640 650
ALVNYVQPIH FSSFENAEKK NRCYEMSSFD EKQATTLLKE RPIEFVNYNK
660 670 680 690 700
HQLSRVYPAG TRFDSSNFMP QLFWNAGCQL VALNFQTLDL AMQLNLGIFE
710 720 730 740 750
YNARSGYLLK PEFMRRSDRR LDPFAESTVD GIIAGTVSIT VLSGQFLTDK
760 770 780 790 800
RANTFVEVDM YGLPADTVRK KFRTKTVRDN GMNPLYDEEP FVFKKVVLPE
810 820 830 840 850
LASIRIAAYE EGGKLIGHRV LPVIGLCPGY RHVNLRSEVG QPIALASLFL
860 870 880 890 900
CVVVKDYVPD DLSNFAEALA NPIKYQSELE KRDIQLSVLT DEAEALGSAD
910 920 930 940 950
EDLSKSFVFV QVGGQKKELR PVESLATSPK HRPSISAAAA MSVDVTVDRT
960 970 980 990 1000
DGGRGEDSIS IVAPSIQHQH SLDQSVSTSI RQVESSQFDV DLVLAEPLEK
1010 1020 1030 1040 1050
ILDHKSVKEK RLEMEKKLES LRKKHDKEKI KIAGQKSSPL EGKKPKFAIT
1060 1070 1080 1090 1100
NKLVKRLSNK SLNCLSPHSE PGVEIPACPL DLGDSSEESA AADAGEDLAG
1110 1120 1130 1140 1150
GSSSLDGRTQ ESRLRSACRE YTSQYRELQE KYHEAIYSAA EKVLKTSQTG
1160 1170 1180 1190 1200
QTKQLKASLD KVTGEVMHQL QEARRNEVKN LATVHRDRDE LIRMKREVAS
1210 1220 1230 1240 1250
SVVERGVAER VRLKQTFDRR TDELQKQHDS VRNALAEHRS KARQILDKEA
1260 1270 1280 1290 1300
ESRSCVSSNG FLVLFHGPHH HGCTGSGSSA LSGNNLTLNL DAGAAGSHSA
1310
ISPAKSHNSI AAAAEMKT
Note: No experimental confirmation available.
Length:1,318
Mass (Da):146,391
Last modified:December 12, 2006 - v3
Checksum:i95C14A73843A325E
GO
Isoform 1 (identifier: P25455-2) [UniParc]FASTAAdd to basket

Also known as: Class-I

The sequence of this isoform differs from the canonical sequence as follows:
     907-913: FVFVQVG → C
     1063-1069: Missing.

Show »
Length:1,305
Mass (Da):144,979
Checksum:iCB5B3ED60B5C1ACC
GO
Isoform A (identifier: P25455-1) [UniParc]FASTAAdd to basket

Also known as: B, Class-II

The sequence of this isoform differs from the canonical sequence as follows:
     907-913: FVFVQVG → C

Show »
Length:1,312
Mass (Da):145,718
Checksum:iB8DD0EF5A48C2665
GO
Isoform C (identifier: P25455-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1063-1069: Missing.

Note: No experimental confirmation available.
Show »
Length:1,311
Mass (Da):145,653
Checksum:i906B2BF56240A89E
GO
Isoform H (identifier: P25455-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     907-912: FVFVQV → L
     1063-1069: Missing.

Note: No experimental confirmation available.
Show »
Length:1,306
Mass (Da):145,046
Checksum:i5439E23B80AA5974
GO
Isoform G (identifier: P25455-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     300-360: Missing.
     1063-1069: Missing.

Note: No experimental confirmation available.
Show »
Length:1,250
Mass (Da):138,774
Checksum:i039C4E8C9E785417
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti508 – 5081G → A in AAA28819 (PubMed:2061323).Curated
Sequence conflicti508 – 5081G → A in AAA28820 (PubMed:2061323).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei300 – 36061Missing in isoform G. CuratedVSP_053949Add
BLAST
Alternative sequencei907 – 9137FVFVQVG → C in isoform 1 and isoform A. 1 PublicationVSP_021970
Alternative sequencei907 – 9126FVFVQV → L in isoform H. CuratedVSP_053950
Alternative sequencei1063 – 10697Missing in isoform 1, isoform C, isoform G and isoform H. 2 PublicationsVSP_004727

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60452 mRNA. Translation: AAA28819.1.
M60453 mRNA. Translation: AAA28820.1.
AE014134 Genomic DNA. Translation: AAN10493.1.
AE014134 Genomic DNA. Translation: AAS64631.1.
AE014134 Genomic DNA. Translation: AAS64632.2.
AE014134 Genomic DNA. Translation: AAS64633.1.
AE014134 Genomic DNA. Translation: AGB92355.1.
AY051657 mRNA. Translation: AAK93081.1.
PIRiA40879.
B40879.
RefSeqiNP_001259818.1. NM_001272889.1. [P25455-6]
NP_476851.2. NM_057503.5. [P25455-1]
NP_476852.1. NM_057504.5. [P25455-1]
NP_995604.2. NM_205882.2. [P25455-5]
NP_995605.1. NM_205883.2. [P25455-3]
NP_995606.1. NM_205884.3. [P25455-4]
UniGeneiDm.4674.

Genome annotation databases

EnsemblMetazoaiFBtr0078050; FBpp0089226; FBgn0004611. [P25455-3]
GeneIDi33204.
KEGGidme:Dmel_CG4574.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60452 mRNA. Translation: AAA28819.1.
M60453 mRNA. Translation: AAA28820.1.
AE014134 Genomic DNA. Translation: AAN10493.1.
AE014134 Genomic DNA. Translation: AAS64631.1.
AE014134 Genomic DNA. Translation: AAS64632.2.
AE014134 Genomic DNA. Translation: AAS64633.1.
AE014134 Genomic DNA. Translation: AGB92355.1.
AY051657 mRNA. Translation: AAK93081.1.
PIRiA40879.
B40879.
RefSeqiNP_001259818.1. NM_001272889.1. [P25455-6]
NP_476851.2. NM_057503.5. [P25455-1]
NP_476852.1. NM_057504.5. [P25455-1]
NP_995604.2. NM_205882.2. [P25455-5]
NP_995605.1. NM_205883.2. [P25455-3]
NP_995606.1. NM_205884.3. [P25455-4]
UniGeneiDm.4674.

3D structure databases

ProteinModelPortaliP25455.
SMRiP25455. Positions 16-468, 568-892.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59460. 1 interaction.
IntActiP25455. 4 interactions.
STRINGi7227.FBpp0089226.

Proteomic databases

PaxDbiP25455.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0078050; FBpp0089226; FBgn0004611. [P25455-3]
GeneIDi33204.
KEGGidme:Dmel_CG4574.

Organism-specific databases

CTDi33204.
FlyBaseiFBgn0004611. Plc21C.

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00760000118936.
InParanoidiP25455.
KOiK05858.
OMAiDANTTQF.
OrthoDBiEOG7WDN1N.
PhylomeDBiP25455.

Enzyme and pathway databases

ReactomeiREACT_282109. G alpha (q) signalling events.
REACT_288405. Synthesis of IP3 and IP4 in the cytosol.
REACT_349107. PLC beta mediated events.
REACT_352779. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
REACT_353102. Acetylcholine regulates insulin secretion.

Miscellaneous databases

ChiTaRSiPlc21C. fly.
GenomeRNAii33204.
NextBioi782409.
PROiP25455.

Gene expression databases

BgeeiP25455.
GenevisibleiP25455. DM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028407. PLC21C.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF87. PTHR10336:SF87. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A Drosophila phospholipase C gene that is expressed in the central nervous system."
    Shortridge R.D., Yoon J., Lending C.R., Bloomquist B.T., Perdew M.H., Pak W.L.
    J. Biol. Chem. 266:12474-12480(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND A), TISSUE SPECIFICITY.
    Tissue: Head.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiPIP1_DROME
AccessioniPrimary (citable) accession number: P25455
Secondary accession number(s): A4UZX5
, M9PC05, Q0E8V1, Q7KTZ7, Q961D5, Q9VPN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: December 12, 2006
Last modified: June 24, 2015
This is version 148 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.