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P25455

- PIP1_DROME

UniProt

P25455 - PIP1_DROME

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase classes I and II

Gene
Plc21C, plc-21, CG4574
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei333 – 3331 By similarity
Active sitei378 – 3781 By similarity
Binding sitei464 – 4641Substrate By similarity
Binding sitei466 – 4661Substrate By similarity
Binding sitei628 – 6281Substrate By similarity
Binding sitei655 – 6551Substrate By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. flight behavior Source: FlyBase
  2. intracellular signal transduction Source: InterPro
  3. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiREACT_180765. Synthesis of IP3 and IP4 in the cytosol.
REACT_220399. PLC beta mediated events.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase classes I and II (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C
Gene namesi
Name:Plc21C
Synonyms:plc-21
ORF Names:CG4574
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0004611. Plc21C.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 131813181-phosphatidylinositol 4,5-bisphosphate phosphodiesterase classes I and IIPRO_0000088511Add
BLAST

Proteomic databases

PaxDbiP25455.

Expressioni

Tissue specificityi

Expressed in neuronal cell bodies of the optic lobe, central brain, and thoracic ganglia in adults, and the brain of larvae.1 Publication

Gene expression databases

BgeeiP25455.

Interactioni

Protein-protein interaction databases

BioGridi59460. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP25455.
SMRiP25455. Positions 16-469, 589-889.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini318 – 466149PI-PLC X-boxAdd
BLAST
Domaini599 – 715117PI-PLC Y-boxAdd
BLAST
Domaini722 – 82099C2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi469 – 47810His-rich

Sequence similaritiesi

Contains 1 C2 domain.

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00730000110266.
InParanoidiP25455.
KOiK05858.
OMAiGSDFVNM.
OrthoDBiEOG7WDN1N.
PhylomeDBiP25455.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028407. PLC21C.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF87. PTHR10336:SF87. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform D (identifier: P25455-3) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MMSAGGTYIS TASVEVPQAL QDGEKFIRWD DDSGTGTPVT MRVDAKGFFL     50
YWVDQNNELD ILDIATIRDV RTGQYAKRPK DNKLRQIVTL GPQDTLEEKT 100
VTVCHGSDFV NMTFVNFCCT RRDIAQLWTD GLIKLAYSLA QLNGSAIMFL 150
QKAHTKLCLQ VDKSGRIPVK NIIKLFAQNK EDRKRVEKAL DVTGLPSGKV 200
DSISVSKFQF EDFYNLYKYL TQRSEVERLF DSIVGNSKRK CMSIAQLVEF 250
LNKTQRDPRL NEILYPYANP ARAKELIQQY EPNKFNAQKG QLSLDGFLRY 300
LMGDDNPIMA PSKLDLCDDM DQPMSHYFIN SSHNTYLTGH QLTGKSSVEI 350
YRQCLLAGCR CVELDFWNGR TEEPVIVHGY TFVPEIFAKD VLEAIAESAF 400
KTSEYPVILS FENHCNPRQQ AKIANYCREI FGDMLLDKPL DSHPLEPNMD 450
LPPPAMLRRK IIIKNKKKHH HHHHHHHHKK PAQVGTPAAN NKLTTANSVD 500
AKAAQQVGLS ASHEDGGVTR STANGDVATG TGTGSAAGTA GHAPPLQQIR 550
QSSKDSTGSS DSDSSSEDES LPNTTPNLPS GNEPPPEKAQ KETEAGAEIS 600
ALVNYVQPIH FSSFENAEKK NRCYEMSSFD EKQATTLLKE RPIEFVNYNK 650
HQLSRVYPAG TRFDSSNFMP QLFWNAGCQL VALNFQTLDL AMQLNLGIFE 700
YNARSGYLLK PEFMRRSDRR LDPFAESTVD GIIAGTVSIT VLSGQFLTDK 750
RANTFVEVDM YGLPADTVRK KFRTKTVRDN GMNPLYDEEP FVFKKVVLPE 800
LASIRIAAYE EGGKLIGHRV LPVIGLCPGY RHVNLRSEVG QPIALASLFL 850
CVVVKDYVPD DLSNFAEALA NPIKYQSELE KRDIQLSVLT DEAEALGSAD 900
EDLSKSFVFV QVGGQKKELR PVESLATSPK HRPSISAAAA MSVDVTVDRT 950
DGGRGEDSIS IVAPSIQHQH SLDQSVSTSI RQVESSQFDV DLVLAEPLEK 1000
ILDHKSVKEK RLEMEKKLES LRKKHDKEKI KIAGQKSSPL EGKKPKFAIT 1050
NKLVKRLSNK SLNCLSPHSE PGVEIPACPL DLGDSSEESA AADAGEDLAG 1100
GSSSLDGRTQ ESRLRSACRE YTSQYRELQE KYHEAIYSAA EKVLKTSQTG 1150
QTKQLKASLD KVTGEVMHQL QEARRNEVKN LATVHRDRDE LIRMKREVAS 1200
SVVERGVAER VRLKQTFDRR TDELQKQHDS VRNALAEHRS KARQILDKEA 1250
ESRSCVSSNG FLVLFHGPHH HGCTGSGSSA LSGNNLTLNL DAGAAGSHSA 1300
ISPAKSHNSI AAAAEMKT 1318

Note: No experimental confirmation available.

Length:1,318
Mass (Da):146,391
Last modified:December 12, 2006 - v3
Checksum:i95C14A73843A325E
GO
Isoform 1 (identifier: P25455-2) [UniParc]FASTAAdd to Basket

Also known as: Class-I

The sequence of this isoform differs from the canonical sequence as follows:
     907-913: FVFVQVG → C
     1063-1069: Missing.

Show »
Length:1,305
Mass (Da):144,979
Checksum:iCB5B3ED60B5C1ACC
GO
Isoform A (identifier: P25455-1) [UniParc]FASTAAdd to Basket

Also known as: B, Class-II

The sequence of this isoform differs from the canonical sequence as follows:
     907-913: FVFVQVG → C

Show »
Length:1,312
Mass (Da):145,718
Checksum:iB8DD0EF5A48C2665
GO
Isoform C (identifier: P25455-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1063-1069: Missing.

Note: No experimental confirmation available.

Show »
Length:1,311
Mass (Da):145,653
Checksum:i906B2BF56240A89E
GO
Isoform H (identifier: P25455-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     907-912: FVFVQV → L
     1063-1069: Missing.

Note: No experimental confirmation available.

Show »
Length:1,306
Mass (Da):145,046
Checksum:i5439E23B80AA5974
GO
Isoform G (identifier: P25455-6) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     300-360: Missing.
     1063-1069: Missing.

Note: No experimental confirmation available.

Show »
Length:1,250
Mass (Da):138,774
Checksum:i039C4E8C9E785417
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei300 – 36061Missing in isoform G. VSP_053949Add
BLAST
Alternative sequencei907 – 9137FVFVQVG → C in isoform 1 and isoform A. VSP_021970
Alternative sequencei907 – 9126FVFVQV → L in isoform H. VSP_053950
Alternative sequencei1063 – 10697Missing in isoform 1, isoform C, isoform G and isoform H. VSP_004727

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti508 – 5081G → A in AAA28819. 1 Publication
Sequence conflicti508 – 5081G → A in AAA28820. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60452 mRNA. Translation: AAA28819.1.
M60453 mRNA. Translation: AAA28820.1.
AE014134 Genomic DNA. Translation: AAN10493.1.
AE014134 Genomic DNA. Translation: AAS64631.1.
AE014134 Genomic DNA. Translation: AAS64632.2.
AE014134 Genomic DNA. Translation: AAS64633.1.
AE014134 Genomic DNA. Translation: AGB92355.1.
AY051657 mRNA. Translation: AAK93081.1.
PIRiA40879.
B40879.
RefSeqiNP_001259818.1. NM_001272889.1. [P25455-6]
NP_476851.2. NM_057503.5. [P25455-1]
NP_476852.1. NM_057504.5. [P25455-1]
NP_995604.2. NM_205882.2. [P25455-5]
NP_995605.1. NM_205883.2. [P25455-3]
NP_995606.1. NM_205884.2. [P25455-4]
UniGeneiDm.4674.

Genome annotation databases

EnsemblMetazoaiFBtr0078050; FBpp0089226; FBgn0004611. [P25455-3]
FBtr0330673; FBpp0303523; FBgn0004611. [P25455-6]
GeneIDi33204.
KEGGidme:Dmel_CG4574.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60452 mRNA. Translation: AAA28819.1 .
M60453 mRNA. Translation: AAA28820.1 .
AE014134 Genomic DNA. Translation: AAN10493.1 .
AE014134 Genomic DNA. Translation: AAS64631.1 .
AE014134 Genomic DNA. Translation: AAS64632.2 .
AE014134 Genomic DNA. Translation: AAS64633.1 .
AE014134 Genomic DNA. Translation: AGB92355.1 .
AY051657 mRNA. Translation: AAK93081.1 .
PIRi A40879.
B40879.
RefSeqi NP_001259818.1. NM_001272889.1. [P25455-6 ]
NP_476851.2. NM_057503.5. [P25455-1 ]
NP_476852.1. NM_057504.5. [P25455-1 ]
NP_995604.2. NM_205882.2. [P25455-5 ]
NP_995605.1. NM_205883.2. [P25455-3 ]
NP_995606.1. NM_205884.2. [P25455-4 ]
UniGenei Dm.4674.

3D structure databases

ProteinModelPortali P25455.
SMRi P25455. Positions 16-469, 589-889.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 59460. 1 interaction.

Proteomic databases

PaxDbi P25455.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0078050 ; FBpp0089226 ; FBgn0004611 . [P25455-3 ]
FBtr0330673 ; FBpp0303523 ; FBgn0004611 . [P25455-6 ]
GeneIDi 33204.
KEGGi dme:Dmel_CG4574.

Organism-specific databases

CTDi 33204.
FlyBasei FBgn0004611. Plc21C.

Phylogenomic databases

eggNOGi NOG149692.
GeneTreei ENSGT00730000110266.
InParanoidi P25455.
KOi K05858.
OMAi GSDFVNM.
OrthoDBi EOG7WDN1N.
PhylomeDBi P25455.

Enzyme and pathway databases

Reactomei REACT_180765. Synthesis of IP3 and IP4 in the cytosol.
REACT_220399. PLC beta mediated events.

Miscellaneous databases

ChiTaRSi Plc21C. drosophila.
GenomeRNAii 33204.
NextBioi 782409.

Gene expression databases

Bgeei P25455.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028407. PLC21C.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF87. PTHR10336:SF87. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEi PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A Drosophila phospholipase C gene that is expressed in the central nervous system."
    Shortridge R.D., Yoon J., Lending C.R., Bloomquist B.T., Perdew M.H., Pak W.L.
    J. Biol. Chem. 266:12474-12480(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND A), TISSUE SPECIFICITY.
    Tissue: Head.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiPIP1_DROME
AccessioniPrimary (citable) accession number: P25455
Secondary accession number(s): A4UZX5
, M9PC05, Q0E8V1, Q7KTZ7, Q961D5, Q9VPN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: December 12, 2006
Last modified: September 3, 2014
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi