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P25455 (PIP1_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase classes I and II

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase C
Gene names
Name:Plc21C
Synonyms:plc-21
ORF Names:CG4574
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1318 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Tissue specificity

Expressed in neuronal cell bodies of the optic lobe, central brain, and thoracic ganglia in adults, and the brain of larvae. Ref.1

Sequence similarities

Contains 1 C2 domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform D (identifier: P25455-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 1 (identifier: P25455-2)

Also known as: Class-I;

The sequence of this isoform differs from the canonical sequence as follows:
     907-913: FVFVQVG → C
     1063-1069: Missing.
Isoform A (identifier: P25455-1)

Also known as: B; Class-II;

The sequence of this isoform differs from the canonical sequence as follows:
     907-913: FVFVQVG → C
Isoform C (identifier: P25455-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1063-1069: Missing.
Note: No experimental confirmation available.
Isoform H (identifier: P25455-5)

The sequence of this isoform differs from the canonical sequence as follows:
     907-912: FVFVQV → L
     1063-1069: Missing.
Note: No experimental confirmation available.
Isoform G (identifier: P25455-6)

The sequence of this isoform differs from the canonical sequence as follows:
     300-360: Missing.
     1063-1069: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 131813181-phosphatidylinositol 4,5-bisphosphate phosphodiesterase classes I and II
PRO_0000088511

Regions

Domain318 – 466149PI-PLC X-box
Domain599 – 715117PI-PLC Y-box
Domain722 – 82099C2
Compositional bias469 – 47810His-rich

Sites

Active site3331 By similarity
Active site3781 By similarity
Binding site4641Substrate By similarity
Binding site4661Substrate By similarity
Binding site6281Substrate By similarity
Binding site6551Substrate By similarity

Natural variations

Alternative sequence300 – 36061Missing in isoform G.
VSP_053949
Alternative sequence907 – 9137FVFVQVG → C in isoform 1 and isoform A.
VSP_021970
Alternative sequence907 – 9126FVFVQV → L in isoform H.
VSP_053950
Alternative sequence1063 – 10697Missing in isoform 1, isoform C, isoform G and isoform H.
VSP_004727

Experimental info

Sequence conflict5081G → A in AAA28819. Ref.1
Sequence conflict5081G → A in AAA28820. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform D [UniParc].

Last modified December 12, 2006. Version 3.
Checksum: 95C14A73843A325E

FASTA1,318146,391
        10         20         30         40         50         60 
MMSAGGTYIS TASVEVPQAL QDGEKFIRWD DDSGTGTPVT MRVDAKGFFL YWVDQNNELD 

        70         80         90        100        110        120 
ILDIATIRDV RTGQYAKRPK DNKLRQIVTL GPQDTLEEKT VTVCHGSDFV NMTFVNFCCT 

       130        140        150        160        170        180 
RRDIAQLWTD GLIKLAYSLA QLNGSAIMFL QKAHTKLCLQ VDKSGRIPVK NIIKLFAQNK 

       190        200        210        220        230        240 
EDRKRVEKAL DVTGLPSGKV DSISVSKFQF EDFYNLYKYL TQRSEVERLF DSIVGNSKRK 

       250        260        270        280        290        300 
CMSIAQLVEF LNKTQRDPRL NEILYPYANP ARAKELIQQY EPNKFNAQKG QLSLDGFLRY 

       310        320        330        340        350        360 
LMGDDNPIMA PSKLDLCDDM DQPMSHYFIN SSHNTYLTGH QLTGKSSVEI YRQCLLAGCR 

       370        380        390        400        410        420 
CVELDFWNGR TEEPVIVHGY TFVPEIFAKD VLEAIAESAF KTSEYPVILS FENHCNPRQQ 

       430        440        450        460        470        480 
AKIANYCREI FGDMLLDKPL DSHPLEPNMD LPPPAMLRRK IIIKNKKKHH HHHHHHHHKK 

       490        500        510        520        530        540 
PAQVGTPAAN NKLTTANSVD AKAAQQVGLS ASHEDGGVTR STANGDVATG TGTGSAAGTA 

       550        560        570        580        590        600 
GHAPPLQQIR QSSKDSTGSS DSDSSSEDES LPNTTPNLPS GNEPPPEKAQ KETEAGAEIS 

       610        620        630        640        650        660 
ALVNYVQPIH FSSFENAEKK NRCYEMSSFD EKQATTLLKE RPIEFVNYNK HQLSRVYPAG 

       670        680        690        700        710        720 
TRFDSSNFMP QLFWNAGCQL VALNFQTLDL AMQLNLGIFE YNARSGYLLK PEFMRRSDRR 

       730        740        750        760        770        780 
LDPFAESTVD GIIAGTVSIT VLSGQFLTDK RANTFVEVDM YGLPADTVRK KFRTKTVRDN 

       790        800        810        820        830        840 
GMNPLYDEEP FVFKKVVLPE LASIRIAAYE EGGKLIGHRV LPVIGLCPGY RHVNLRSEVG 

       850        860        870        880        890        900 
QPIALASLFL CVVVKDYVPD DLSNFAEALA NPIKYQSELE KRDIQLSVLT DEAEALGSAD 

       910        920        930        940        950        960 
EDLSKSFVFV QVGGQKKELR PVESLATSPK HRPSISAAAA MSVDVTVDRT DGGRGEDSIS 

       970        980        990       1000       1010       1020 
IVAPSIQHQH SLDQSVSTSI RQVESSQFDV DLVLAEPLEK ILDHKSVKEK RLEMEKKLES 

      1030       1040       1050       1060       1070       1080 
LRKKHDKEKI KIAGQKSSPL EGKKPKFAIT NKLVKRLSNK SLNCLSPHSE PGVEIPACPL 

      1090       1100       1110       1120       1130       1140 
DLGDSSEESA AADAGEDLAG GSSSLDGRTQ ESRLRSACRE YTSQYRELQE KYHEAIYSAA 

      1150       1160       1170       1180       1190       1200 
EKVLKTSQTG QTKQLKASLD KVTGEVMHQL QEARRNEVKN LATVHRDRDE LIRMKREVAS 

      1210       1220       1230       1240       1250       1260 
SVVERGVAER VRLKQTFDRR TDELQKQHDS VRNALAEHRS KARQILDKEA ESRSCVSSNG 

      1270       1280       1290       1300       1310 
FLVLFHGPHH HGCTGSGSSA LSGNNLTLNL DAGAAGSHSA ISPAKSHNSI AAAAEMKT 

« Hide

Isoform 1 (Class-I) [UniParc].

Checksum: CB5B3ED60B5C1ACC
Show »

FASTA1,305144,979
Isoform A (B) (Class-II) [UniParc].

Checksum: B8DD0EF5A48C2665
Show »

FASTA1,312145,718
Isoform C [UniParc].

Checksum: 906B2BF56240A89E
Show »

FASTA1,311145,653
Isoform H [UniParc].

Checksum: 5439E23B80AA5974
Show »

FASTA1,306145,046
Isoform G [UniParc].

Checksum: 039C4E8C9E785417
Show »

FASTA1,250138,774

References

« Hide 'large scale' references
[1]"A Drosophila phospholipase C gene that is expressed in the central nervous system."
Shortridge R.D., Yoon J., Lending C.R., Bloomquist B.T., Perdew M.H., Pak W.L.
J. Biol. Chem. 266:12474-12480(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND A), TISSUE SPECIFICITY.
Tissue: Head.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
Strain: Berkeley.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60452 mRNA. Translation: AAA28819.1.
M60453 mRNA. Translation: AAA28820.1.
AE014134 Genomic DNA. Translation: AAN10493.1.
AE014134 Genomic DNA. Translation: AAS64631.1.
AE014134 Genomic DNA. Translation: AAS64632.2.
AE014134 Genomic DNA. Translation: AAS64633.1.
AE014134 Genomic DNA. Translation: AGB92355.1.
AY051657 mRNA. Translation: AAK93081.1.
PIRA40879.
B40879.
RefSeqNP_001259818.1. NM_001272889.1. [P25455-6]
NP_476851.2. NM_057503.5. [P25455-1]
NP_476852.1. NM_057504.5. [P25455-1]
NP_995604.2. NM_205882.2. [P25455-5]
NP_995605.1. NM_205883.2. [P25455-3]
NP_995606.1. NM_205884.2. [P25455-4]
UniGeneDm.4674.

3D structure databases

ProteinModelPortalP25455.
SMRP25455. Positions 16-469, 589-889.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid59460. 1 interaction.

Proteomic databases

PaxDbP25455.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0078050; FBpp0089226; FBgn0004611. [P25455-3]
FBtr0330673; FBpp0303523; FBgn0004611. [P25455-6]
GeneID33204.
KEGGdme:Dmel_CG4574.

Organism-specific databases

CTD33204.
FlyBaseFBgn0004611. Plc21C.

Phylogenomic databases

eggNOGNOG149692.
GeneTreeENSGT00730000110266.
InParanoidP25455.
KOK05858.
OMAGSDFVNM.
OrthoDBEOG7WDN1N.
PhylomeDBP25455.

Gene expression databases

BgeeP25455.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028407. PLC21C.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF87. PTHR10336:SF87. 1 hit.
PfamPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPlc21C. drosophila.
GenomeRNAi33204.
NextBio782409.

Entry information

Entry namePIP1_DROME
AccessionPrimary (citable) accession number: P25455
Secondary accession number(s): A4UZX5 expand/collapse secondary AC list , M9PC05, Q0E8V1, Q7KTZ7, Q961D5, Q9VPN9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: December 12, 2006
Last modified: July 9, 2014
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase