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P25455

- PIP1_DROME

UniProt

P25455 - PIP1_DROME

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase classes I and II

Gene

Plc21C

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 3 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei333 – 3331PROSITE-ProRule annotation
    Active sitei378 – 3781PROSITE-ProRule annotation
    Binding sitei464 – 4641SubstrateBy similarity
    Binding sitei466 – 4661SubstrateBy similarity
    Binding sitei628 – 6281SubstrateBy similarity
    Binding sitei655 – 6551SubstrateBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. flight behavior Source: FlyBase
    2. intracellular signal transduction Source: InterPro
    3. lipid catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_180765. Synthesis of IP3 and IP4 in the cytosol.
    REACT_220399. PLC beta mediated events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase classes I and II (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C
    Gene namesi
    Name:Plc21C
    Synonyms:plc-21
    ORF Names:CG4574
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0004611. Plc21C.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 131813181-phosphatidylinositol 4,5-bisphosphate phosphodiesterase classes I and IIPRO_0000088511Add
    BLAST

    Proteomic databases

    PaxDbiP25455.

    Expressioni

    Tissue specificityi

    Expressed in neuronal cell bodies of the optic lobe, central brain, and thoracic ganglia in adults, and the brain of larvae.1 Publication

    Gene expression databases

    BgeeiP25455.

    Interactioni

    Protein-protein interaction databases

    BioGridi59460. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP25455.
    SMRiP25455. Positions 16-469, 589-889.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini318 – 466149PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini599 – 715117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini722 – 82099C2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi469 – 47810His-rich

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    GeneTreeiENSGT00730000110266.
    InParanoidiP25455.
    KOiK05858.
    OMAiGSDFVNM.
    OrthoDBiEOG7WDN1N.
    PhylomeDBiP25455.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR014815. PLC-beta_C.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR028407. PLC21C.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF87. PTHR10336:SF87. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF08703. PLC-beta_C. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 2 hits.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequences (6)i

    Sequence statusi: Complete.

    This entry describes 6 isoformsi produced by alternative splicing. Align

    Isoform D (identifier: P25455-3) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMSAGGTYIS TASVEVPQAL QDGEKFIRWD DDSGTGTPVT MRVDAKGFFL     50
    YWVDQNNELD ILDIATIRDV RTGQYAKRPK DNKLRQIVTL GPQDTLEEKT 100
    VTVCHGSDFV NMTFVNFCCT RRDIAQLWTD GLIKLAYSLA QLNGSAIMFL 150
    QKAHTKLCLQ VDKSGRIPVK NIIKLFAQNK EDRKRVEKAL DVTGLPSGKV 200
    DSISVSKFQF EDFYNLYKYL TQRSEVERLF DSIVGNSKRK CMSIAQLVEF 250
    LNKTQRDPRL NEILYPYANP ARAKELIQQY EPNKFNAQKG QLSLDGFLRY 300
    LMGDDNPIMA PSKLDLCDDM DQPMSHYFIN SSHNTYLTGH QLTGKSSVEI 350
    YRQCLLAGCR CVELDFWNGR TEEPVIVHGY TFVPEIFAKD VLEAIAESAF 400
    KTSEYPVILS FENHCNPRQQ AKIANYCREI FGDMLLDKPL DSHPLEPNMD 450
    LPPPAMLRRK IIIKNKKKHH HHHHHHHHKK PAQVGTPAAN NKLTTANSVD 500
    AKAAQQVGLS ASHEDGGVTR STANGDVATG TGTGSAAGTA GHAPPLQQIR 550
    QSSKDSTGSS DSDSSSEDES LPNTTPNLPS GNEPPPEKAQ KETEAGAEIS 600
    ALVNYVQPIH FSSFENAEKK NRCYEMSSFD EKQATTLLKE RPIEFVNYNK 650
    HQLSRVYPAG TRFDSSNFMP QLFWNAGCQL VALNFQTLDL AMQLNLGIFE 700
    YNARSGYLLK PEFMRRSDRR LDPFAESTVD GIIAGTVSIT VLSGQFLTDK 750
    RANTFVEVDM YGLPADTVRK KFRTKTVRDN GMNPLYDEEP FVFKKVVLPE 800
    LASIRIAAYE EGGKLIGHRV LPVIGLCPGY RHVNLRSEVG QPIALASLFL 850
    CVVVKDYVPD DLSNFAEALA NPIKYQSELE KRDIQLSVLT DEAEALGSAD 900
    EDLSKSFVFV QVGGQKKELR PVESLATSPK HRPSISAAAA MSVDVTVDRT 950
    DGGRGEDSIS IVAPSIQHQH SLDQSVSTSI RQVESSQFDV DLVLAEPLEK 1000
    ILDHKSVKEK RLEMEKKLES LRKKHDKEKI KIAGQKSSPL EGKKPKFAIT 1050
    NKLVKRLSNK SLNCLSPHSE PGVEIPACPL DLGDSSEESA AADAGEDLAG 1100
    GSSSLDGRTQ ESRLRSACRE YTSQYRELQE KYHEAIYSAA EKVLKTSQTG 1150
    QTKQLKASLD KVTGEVMHQL QEARRNEVKN LATVHRDRDE LIRMKREVAS 1200
    SVVERGVAER VRLKQTFDRR TDELQKQHDS VRNALAEHRS KARQILDKEA 1250
    ESRSCVSSNG FLVLFHGPHH HGCTGSGSSA LSGNNLTLNL DAGAAGSHSA 1300
    ISPAKSHNSI AAAAEMKT 1318

    Note: No experimental confirmation available.

    Length:1,318
    Mass (Da):146,391
    Last modified:December 12, 2006 - v3
    Checksum:i95C14A73843A325E
    GO
    Isoform 1 (identifier: P25455-2) [UniParc]FASTAAdd to Basket

    Also known as: Class-I

    The sequence of this isoform differs from the canonical sequence as follows:
         907-913: FVFVQVG → C
         1063-1069: Missing.

    Show »
    Length:1,305
    Mass (Da):144,979
    Checksum:iCB5B3ED60B5C1ACC
    GO
    Isoform A (identifier: P25455-1) [UniParc]FASTAAdd to Basket

    Also known as: B, Class-II

    The sequence of this isoform differs from the canonical sequence as follows:
         907-913: FVFVQVG → C

    Show »
    Length:1,312
    Mass (Da):145,718
    Checksum:iB8DD0EF5A48C2665
    GO
    Isoform C (identifier: P25455-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1063-1069: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,311
    Mass (Da):145,653
    Checksum:i906B2BF56240A89E
    GO
    Isoform H (identifier: P25455-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         907-912: FVFVQV → L
         1063-1069: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,306
    Mass (Da):145,046
    Checksum:i5439E23B80AA5974
    GO
    Isoform G (identifier: P25455-6) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         300-360: Missing.
         1063-1069: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,250
    Mass (Da):138,774
    Checksum:i039C4E8C9E785417
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti508 – 5081G → A in AAA28819. (PubMed:2061323)Curated
    Sequence conflicti508 – 5081G → A in AAA28820. (PubMed:2061323)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei300 – 36061Missing in isoform G. CuratedVSP_053949Add
    BLAST
    Alternative sequencei907 – 9137FVFVQVG → C in isoform 1 and isoform A. 1 PublicationVSP_021970
    Alternative sequencei907 – 9126FVFVQV → L in isoform H. CuratedVSP_053950
    Alternative sequencei1063 – 10697Missing in isoform 1, isoform C, isoform G and isoform H. 2 PublicationsVSP_004727

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60452 mRNA. Translation: AAA28819.1.
    M60453 mRNA. Translation: AAA28820.1.
    AE014134 Genomic DNA. Translation: AAN10493.1.
    AE014134 Genomic DNA. Translation: AAS64631.1.
    AE014134 Genomic DNA. Translation: AAS64632.2.
    AE014134 Genomic DNA. Translation: AAS64633.1.
    AE014134 Genomic DNA. Translation: AGB92355.1.
    AY051657 mRNA. Translation: AAK93081.1.
    PIRiA40879.
    B40879.
    RefSeqiNP_001259818.1. NM_001272889.1. [P25455-6]
    NP_476851.2. NM_057503.5. [P25455-1]
    NP_476852.1. NM_057504.5. [P25455-1]
    NP_995604.2. NM_205882.2. [P25455-5]
    NP_995605.1. NM_205883.2. [P25455-3]
    NP_995606.1. NM_205884.2. [P25455-4]
    UniGeneiDm.4674.

    Genome annotation databases

    EnsemblMetazoaiFBtr0078050; FBpp0089226; FBgn0004611. [P25455-3]
    FBtr0330673; FBpp0303523; FBgn0004611. [P25455-6]
    GeneIDi33204.
    KEGGidme:Dmel_CG4574.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60452 mRNA. Translation: AAA28819.1 .
    M60453 mRNA. Translation: AAA28820.1 .
    AE014134 Genomic DNA. Translation: AAN10493.1 .
    AE014134 Genomic DNA. Translation: AAS64631.1 .
    AE014134 Genomic DNA. Translation: AAS64632.2 .
    AE014134 Genomic DNA. Translation: AAS64633.1 .
    AE014134 Genomic DNA. Translation: AGB92355.1 .
    AY051657 mRNA. Translation: AAK93081.1 .
    PIRi A40879.
    B40879.
    RefSeqi NP_001259818.1. NM_001272889.1. [P25455-6 ]
    NP_476851.2. NM_057503.5. [P25455-1 ]
    NP_476852.1. NM_057504.5. [P25455-1 ]
    NP_995604.2. NM_205882.2. [P25455-5 ]
    NP_995605.1. NM_205883.2. [P25455-3 ]
    NP_995606.1. NM_205884.2. [P25455-4 ]
    UniGenei Dm.4674.

    3D structure databases

    ProteinModelPortali P25455.
    SMRi P25455. Positions 16-469, 589-889.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 59460. 1 interaction.

    Proteomic databases

    PaxDbi P25455.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0078050 ; FBpp0089226 ; FBgn0004611 . [P25455-3 ]
    FBtr0330673 ; FBpp0303523 ; FBgn0004611 . [P25455-6 ]
    GeneIDi 33204.
    KEGGi dme:Dmel_CG4574.

    Organism-specific databases

    CTDi 33204.
    FlyBasei FBgn0004611. Plc21C.

    Phylogenomic databases

    eggNOGi NOG149692.
    GeneTreei ENSGT00730000110266.
    InParanoidi P25455.
    KOi K05858.
    OMAi GSDFVNM.
    OrthoDBi EOG7WDN1N.
    PhylomeDBi P25455.

    Enzyme and pathway databases

    Reactomei REACT_180765. Synthesis of IP3 and IP4 in the cytosol.
    REACT_220399. PLC beta mediated events.

    Miscellaneous databases

    ChiTaRSi Plc21C. drosophila.
    GenomeRNAii 33204.
    NextBioi 782409.

    Gene expression databases

    Bgeei P25455.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR014815. PLC-beta_C.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR028407. PLC21C.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF87. PTHR10336:SF87. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF08703. PLC-beta_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 2 hits.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A Drosophila phospholipase C gene that is expressed in the central nervous system."
      Shortridge R.D., Yoon J., Lending C.R., Bloomquist B.T., Perdew M.H., Pak W.L.
      J. Biol. Chem. 266:12474-12480(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND A), TISSUE SPECIFICITY.
      Tissue: Head.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
      Strain: Berkeley.
      Tissue: Embryo.

    Entry informationi

    Entry nameiPIP1_DROME
    AccessioniPrimary (citable) accession number: P25455
    Secondary accession number(s): A4UZX5
    , M9PC05, Q0E8V1, Q7KTZ7, Q961D5, Q9VPN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 140 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3