DNA repair protein RAD51 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P25454 (RAD51_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA repair protein RAD51

Gene names

Name:	RAD51
Ordered Locus Names:	YER095W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required both for recombination and for the repair of DNA damage caused by X-rays. Its function may be modulated by interaction with other repair proteins. RAD52 interacts directly with RAD51, via its C-terminus. Forms a nucleoprotein filament with DNA as an early intermediate in recombination. Ref.9
Subunit structure	Part of a repair/recombination complex that includes RAD51, RAD52 and RAD54. Interacts with HED1, RDH54 and SAW1. Ref.6 Ref.7 Ref.9 Ref.10
Subcellular location	Nucleus. Chromosome. Note: Localizes as foci on meiotic chromosomes. Ref.9
Developmental stage	RAD51 is cell cycle regulated, peaking around the G1-to-S transition.
Induction	By X-rays.
Miscellaneous	Present with 6960 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the RecA family. RAD51 subfamily. Contains 1 HhH domain.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Cellular component	Chromosome Nucleus
Ligand	ATP-binding Nucleotide-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	double-strand break repair via homologous recombination Traceable author statement. Source: BHF-UCL heteroduplex formation Inferred from direct assay. Source: SGD meiotic joint molecule formation Inferred from mutant phenotype Ref.3. Source: SGD strand invasion Inferred from direct assay. Source: SGD telomere maintenance via recombination Inferred from mutant phenotype. Source: SGD
Cellular component	condensed nuclear chromosome Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-dependent ATPase activity Inferred from direct assay. Source: SGD damaged DNA binding Inferred from electronic annotation. Source: InterPro identical protein binding Inferred from physical interaction. Source: IntAct recombinase activity Inferred from direct assay. Source: SGD single-stranded DNA binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-14709,EBI-14709

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 400

400

DNA repair protein RAD51

PRO_0000122925

Regions

Nucleotide binding

185 – 192

ATP Potential

Secondary structure

400

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P25454 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 66561B60122A39A2
Show »

FASTA

400

42,963

        10         20         30         40         50         60 
MSQVQEQHIS ESQLQYGNGS LMSTVPADLS QSVVDGNGNG SSEDIEATNG SGDGGGLQEQ 

        70         80         90        100        110        120 
AEAQGEMEDE AYDEAALGSF VPIEKLQVNG ITMADVKKLR ESGLHTAEAV AYAPRKDLLE 

       130        140        150        160        170        180 
IKGISEAKAD KLLNEAARLV PMGFVTAADF HMRRSELICL TTGSKNLDTL LGGGVETGSI 

       190        200        210        220        230        240 
TELFGEFRTG KSQLCHTLAV TCQIPLDIGG GEGKCLYIDT EGTFRPVRLV SIAQRFGLDP 

       250        260        270        280        290        300 
DDALNNVAYA RAYNADHQLR LLDAAAQMMS ESRFSLIVVD SVMALYRTDF SGRGELSARQ 

       310        320        330        340        350        360 
MHLAKFMRAL QRLADQFGVA VVVTNQVVAQ VDGGMAFNPD PKKPIGGNIM AHSSTTRLGF 

       370        380        390        400 
KKGKGCQRLC KVVDSPCLPE AECVFAIYED GVGDPREEDE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and transcriptional regulation of the yeast recombinational repair gene RAD51." Basile G.M., Aker M., Mortimer R.K. Mol. Cell. Biol. 12:3235-3246(1992) [PubMed: 1620128] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Semidominant suppressors of Srs2 helicase mutations of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to procaryotic RecA proteins." Aboussekhra A., Chanet R., Adjiri A., Fabre F. Mol. Cell. Biol. 12:3224-3234(1992) [PubMed: 1620127] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein." Shinohara A., Ogawa H., Ogawa T. Cell 69:457-470(1992) [PubMed: 1581961] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins." Petukhova G., Stratton S., Sung P. Nature 393:91-94(1998) [PubMed: 9590697] [Abstract] Cited for: INTERACTION WITH RAD54.
[7]	"Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1." Petukhova G., Sung P., Klein H. Genes Dev. 14:2206-2215(2000) [PubMed: 10970884] [Abstract] Cited for: INTERACTION WITH RDH54.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Budding yeast Hed1 down-regulates the mitotic recombination machinery when meiotic recombination is impaired." Tsubouchi H., Roeder G.S. Genes Dev. 20:1766-1775(2006) [PubMed: 16818607] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RAD51.
[10]	"Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates." Li F., Dong J., Pan X., Oum J.-H., Boeke J.D., Lee S.E. Mol. Cell 30:325-335(2008) [PubMed: 18471978] [Abstract] Cited for: INTERACTION WITH SAW1.
[11]	"Crystal structure of a Rad51 filament." Conway A.B., Lynch T.W., Zhang Y., Fortin G.S., Fung C.W., Symington L.S., Rice P.A. Nat. Struct. Mol. Biol. 11:791-796(2004) [PubMed: 15235592] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.25 ANGSTROMS) OF 81-400.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X64270 Genomic DNA. Translation: CAA45563.1.
M88470 Genomic DNA. Translation: AAA34948.1.
D10023 Genomic DNA. Translation: BAA00913.1.
U18839 Genomic DNA. Translation: AAB64650.1.
BK006939 Genomic DNA. Translation: DAA07757.1.

PIR

A44348.

RefSeq

NP_011021.1. NM_001178986.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SZP	X-ray	3.25	A/B/C/D/E/F	81-400	[»]
3LDA	X-ray	2.50	A	1-400	[»]

ProteinModelPortal

P25454.

SMR

P25454. Positions 81-394.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-195N.

IntAct

P25454. 45 interactions.

MINT

MINT-386096.

STRING

P25454.

Proteomic databases

PeptideAtlas

P25454.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YER095W; YER095W; YER095W.

GeneID

856831.

KEGG

sce:YER095W.

NMPDR

fig|4932.3.peg.2096.

Organism-specific databases

CYGD

YER095w.

SGD

S000000897. RAD51.

Phylogenomic databases

eggNOG

fuNOG04550.

GeneTree

EFGT00050000002762.

HOGENOM

HBG413235.

OMA

SAMMAES.

OrthoDB

EOG41CB4Z.

Gene expression databases

ArrayExpress

P25454.

Genevestigator

P25454.

GermOnline

YER095W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR003593. ATPase_AAA+_core.
IPR011941. DNA_recomb/repair_Rad51.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR020588. DNA_recomb_RecA/RadB_ATP-bd.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]

K04482.

PANTHER

PTHR22942:SF12. PTHR22942:SF12. 1 hit.

Pfam

PF08423. Rad51. 1 hit.
[Graphical view]

PIRSF

PIRSF005856. Rad51. 1 hit.

SMART

SM00382. AAA. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]

SUPFAM

SSF47794. Rad51_N. 1 hit.

TIGRFAMs

TIGR02239. Recomb_RAD51. 1 hit.

PROSITE

PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

983130.

Entry information

Entry name

RAD51_YEAST

Accession

Primary (citable) accession number: P25454
Secondary accession number(s): D3DM03

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	December 14, 2011
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents