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Protein

DNA repair protein RAD51

Gene

RAD51

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required both for recombination and for the repair of DNA damage caused by X-rays. Its function may be modulated by interaction with other repair proteins. RAD52 interacts directly with RAD51, via its C-terminus. Forms a nucleoprotein filament with DNA as an early intermediate in recombination.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi185 – 1928ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA-dependent ATPase activity Source: SGD
  • double-stranded DNA binding Source: InterPro
  • identical protein binding Source: IntAct
  • recombinase activity Source: SGD
  • single-stranded DNA binding Source: SGD

GO - Biological processi

  • DNA recombination Source: SGD
  • double-strand break repair via homologous recombination Source: BHF-UCL
  • heteroduplex formation Source: SGD
  • homologous recombination-dependent replication fork processing Source: InterPro
  • meiotic joint molecule formation Source: SGD
  • reciprocal meiotic recombination Source: SGD
  • strand invasion Source: SGD
  • telomere maintenance via recombination Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30262-MONOMER.
ReactomeiR-SCE-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD51
Gene namesi
Name:RAD51
Ordered Locus Names:YER095W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER095W.
SGDiS000000897. RAD51.

Subcellular locationi

  • Nucleus 1 Publication
  • Chromosome 1 Publication

  • Note: Localizes as foci on meiotic chromosomes.

GO - Cellular componenti

  • condensed nuclear chromosome Source: SGD
  • nuclear chromosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400DNA repair protein RAD51PRO_0000122925Add
BLAST

Proteomic databases

MaxQBiP25454.
PeptideAtlasiP25454.

PTM databases

iPTMnetiP25454.

Expressioni

Developmental stagei

RAD51 is cell cycle regulated, peaking around the G1-to-S transition.

Inductioni

By X-rays.

Interactioni

Subunit structurei

Part of a repair/recombination complex that includes RAD51, RAD52 and RAD54. Interacts with HED1, RDH54 and SAW1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-14709,EBI-14709

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi36841. 398 interactions.
DIPiDIP-195N.
IntActiP25454. 43 interactions.
MINTiMINT-386096.

Structurei

Secondary structure

1
400
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi83 – 864Combined sources
Helixi93 – 1019Combined sources
Helixi107 – 1126Combined sources
Helixi115 – 1195Combined sources
Helixi126 – 13914Combined sources
Helixi147 – 1559Combined sources
Helixi165 – 1706Combined sources
Turni171 – 1733Combined sources
Beta strandi174 – 1763Combined sources
Beta strandi179 – 1868Combined sources
Helixi191 – 20111Combined sources
Helixi206 – 2083Combined sources
Beta strandi212 – 22211Combined sources
Helixi226 – 23510Combined sources
Helixi240 – 2456Combined sources
Beta strandi247 – 2515Combined sources
Helixi255 – 27117Combined sources
Beta strandi274 – 2807Combined sources
Helixi282 – 2854Combined sources
Helixi296 – 31722Combined sources
Beta strandi320 – 3267Combined sources
Helixi349 – 3535Combined sources
Beta strandi355 – 3628Combined sources
Beta strandi367 – 3748Combined sources
Beta strandi376 – 3783Combined sources
Beta strandi382 – 3887Combined sources
Beta strandi391 – 3933Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SZPX-ray3.25A/B/C/D/E/F81-400[»]
3LDAX-ray2.50A1-400[»]
ProteinModelPortaliP25454.
SMRiP25454. Positions 81-394.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25454.

Family & Domainsi

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated
Contains 1 HhH domain.Curated

Phylogenomic databases

GeneTreeiENSGT00770000120539.
HOGENOMiHOG000227426.
InParanoidiP25454.
KOiK04482.
OMAiLGRFLRM.
OrthoDBiEOG7TN02X.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011941. DNA_recomb/repair_Rad51.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02239. recomb_RAD51. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25454-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQVQEQHIS ESQLQYGNGS LMSTVPADLS QSVVDGNGNG SSEDIEATNG
60 70 80 90 100
SGDGGGLQEQ AEAQGEMEDE AYDEAALGSF VPIEKLQVNG ITMADVKKLR
110 120 130 140 150
ESGLHTAEAV AYAPRKDLLE IKGISEAKAD KLLNEAARLV PMGFVTAADF
160 170 180 190 200
HMRRSELICL TTGSKNLDTL LGGGVETGSI TELFGEFRTG KSQLCHTLAV
210 220 230 240 250
TCQIPLDIGG GEGKCLYIDT EGTFRPVRLV SIAQRFGLDP DDALNNVAYA
260 270 280 290 300
RAYNADHQLR LLDAAAQMMS ESRFSLIVVD SVMALYRTDF SGRGELSARQ
310 320 330 340 350
MHLAKFMRAL QRLADQFGVA VVVTNQVVAQ VDGGMAFNPD PKKPIGGNIM
360 370 380 390 400
AHSSTTRLGF KKGKGCQRLC KVVDSPCLPE AECVFAIYED GVGDPREEDE
Length:400
Mass (Da):42,963
Last modified:May 1, 1992 - v1
Checksum:i66561B60122A39A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64270 Genomic DNA. Translation: CAA45563.1.
M88470 Genomic DNA. Translation: AAA34948.1.
D10023 Genomic DNA. Translation: BAA00913.1.
U18839 Genomic DNA. Translation: AAB64650.1.
BK006939 Genomic DNA. Translation: DAA07757.1.
PIRiA44348.
RefSeqiNP_011021.3. NM_001178986.3.

Genome annotation databases

EnsemblFungiiYER095W; YER095W; YER095W.
GeneIDi856831.
KEGGisce:YER095W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64270 Genomic DNA. Translation: CAA45563.1.
M88470 Genomic DNA. Translation: AAA34948.1.
D10023 Genomic DNA. Translation: BAA00913.1.
U18839 Genomic DNA. Translation: AAB64650.1.
BK006939 Genomic DNA. Translation: DAA07757.1.
PIRiA44348.
RefSeqiNP_011021.3. NM_001178986.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SZPX-ray3.25A/B/C/D/E/F81-400[»]
3LDAX-ray2.50A1-400[»]
ProteinModelPortaliP25454.
SMRiP25454. Positions 81-394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36841. 398 interactions.
DIPiDIP-195N.
IntActiP25454. 43 interactions.
MINTiMINT-386096.

PTM databases

iPTMnetiP25454.

Proteomic databases

MaxQBiP25454.
PeptideAtlasiP25454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER095W; YER095W; YER095W.
GeneIDi856831.
KEGGisce:YER095W.

Organism-specific databases

EuPathDBiFungiDB:YER095W.
SGDiS000000897. RAD51.

Phylogenomic databases

GeneTreeiENSGT00770000120539.
HOGENOMiHOG000227426.
InParanoidiP25454.
KOiK04482.
OMAiLGRFLRM.
OrthoDBiEOG7TN02X.

Enzyme and pathway databases

BioCyciYEAST:G3O-30262-MONOMER.
ReactomeiR-SCE-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.

Miscellaneous databases

EvolutionaryTraceiP25454.
PROiP25454.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011941. DNA_recomb/repair_Rad51.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02239. recomb_RAD51. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and transcriptional regulation of the yeast recombinational repair gene RAD51."
    Basile G.M., Aker M., Mortimer R.K.
    Mol. Cell. Biol. 12:3235-3246(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Semidominant suppressors of Srs2 helicase mutations of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to procaryotic RecA proteins."
    Aboussekhra A., Chanet R., Adjiri A., Fabre F.
    Mol. Cell. Biol. 12:3224-3234(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein."
    Shinohara A., Ogawa H., Ogawa T.
    Cell 69:457-470(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins."
    Petukhova G., Stratton S., Sung P.
    Nature 393:91-94(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAD54.
  7. "Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1."
    Petukhova G., Sung P., Klein H.
    Genes Dev. 14:2206-2215(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RDH54.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Budding yeast Hed1 down-regulates the mitotic recombination machinery when meiotic recombination is impaired."
    Tsubouchi H., Roeder G.S.
    Genes Dev. 20:1766-1775(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RAD51.
  10. "Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates."
    Li F., Dong J., Pan X., Oum J.-H., Boeke J.D., Lee S.E.
    Mol. Cell 30:325-335(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SAW1.
  11. Cited for: X-RAY CRYSTALLOGRAPHY (3.25 ANGSTROMS) OF 81-400.

Entry informationi

Entry nameiRAD51_YEAST
AccessioniPrimary (citable) accession number: P25454
Secondary accession number(s): D3DM03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 8, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6960 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.