Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA repair protein RAD51

Gene

RAD51

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required both for recombination and for the repair of DNA damage caused by X-rays. Its function may be modulated by interaction with other repair proteins. RAD52 interacts directly with RAD51, via its C-terminus. Forms a nucleoprotein filament with DNA as an early intermediate in recombination.1 Publication

Miscellaneous

Present with 6960 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi185 – 192ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA-dependent ATPase activity Source: SGD
  • double-stranded DNA binding Source: GO_Central
  • four-way junction DNA binding Source: GO_Central
  • identical protein binding Source: IntAct
  • recombinase activity Source: SGD
  • single-stranded DNA binding Source: SGD

GO - Biological processi

  • chromosome organization involved in meiotic cell cycle Source: GO_Central
  • DNA recombinase assembly Source: GO_Central
  • DNA recombination Source: SGD
  • double-strand break repair via homologous recombination Source: BHF-UCL
  • heteroduplex formation Source: SGD
  • meiotic joint molecule formation Source: SGD
  • mitotic recombination-dependent replication fork processing Source: InterPro
  • reciprocal meiotic recombination Source: SGD
  • response to ionizing radiation Source: GO_Central
  • strand invasion Source: SGD
  • telomere maintenance via recombination Source: SGD

Keywordsi

Biological processDNA damage, DNA recombination, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30262-MONOMER.
ReactomeiR-SCE-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD51
Gene namesi
Name:RAD51
Ordered Locus Names:YER095W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER095W.
SGDiS000000897. RAD51.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001229251 – 400DNA repair protein RAD51Add BLAST400

Proteomic databases

MaxQBiP25454.
PRIDEiP25454.

PTM databases

iPTMnetiP25454.

Expressioni

Developmental stagei

RAD51 is cell cycle regulated, peaking around the G1-to-S transition.

Inductioni

By X-rays.

Interactioni

Subunit structurei

Part of a repair/recombination complex that includes RAD51, RAD52 and RAD54. Interacts with HED1, RDH54 and SAW1.4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi36841. 482 interactors.
DIPiDIP-195N.
IntActiP25454. 45 interactors.
MINTiMINT-386096.
STRINGi4932.YER095W.

Structurei

Secondary structure

1400
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi83 – 86Combined sources4
Helixi93 – 101Combined sources9
Helixi107 – 112Combined sources6
Helixi115 – 119Combined sources5
Helixi126 – 139Combined sources14
Helixi147 – 155Combined sources9
Helixi165 – 170Combined sources6
Turni171 – 173Combined sources3
Beta strandi174 – 176Combined sources3
Beta strandi179 – 186Combined sources8
Helixi191 – 201Combined sources11
Helixi206 – 208Combined sources3
Beta strandi212 – 222Combined sources11
Helixi226 – 235Combined sources10
Helixi240 – 245Combined sources6
Beta strandi247 – 251Combined sources5
Helixi255 – 271Combined sources17
Beta strandi274 – 280Combined sources7
Helixi282 – 285Combined sources4
Helixi296 – 317Combined sources22
Beta strandi320 – 326Combined sources7
Helixi349 – 353Combined sources5
Beta strandi355 – 362Combined sources8
Beta strandi367 – 374Combined sources8
Beta strandi376 – 378Combined sources3
Beta strandi382 – 388Combined sources7
Beta strandi391 – 393Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SZPX-ray3.25A/B/C/D/E/F81-400[»]
3LDAX-ray2.50A1-400[»]
ProteinModelPortaliP25454.
SMRiP25454.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25454.

Family & Domainsi

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00890000139508.
HOGENOMiHOG000227426.
InParanoidiP25454.
KOiK04482.
OMAiVGHTATF.
OrthoDBiEOG092C3DRU.

Family and domain databases

CDDicd01123. Rad51_DMC1_radA. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR011941. DNA_recomb/repair_Rad51.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR027417. P-loop_NTPase.
IPR033925. Rad51_DMC1_RadA.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
PfamiView protein in Pfam
PF08423. Rad51. 1 hit.
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02239. recomb_RAD51. 1 hit.
PROSITEiView protein in PROSITE
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P25454-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQVQEQHIS ESQLQYGNGS LMSTVPADLS QSVVDGNGNG SSEDIEATNG
60 70 80 90 100
SGDGGGLQEQ AEAQGEMEDE AYDEAALGSF VPIEKLQVNG ITMADVKKLR
110 120 130 140 150
ESGLHTAEAV AYAPRKDLLE IKGISEAKAD KLLNEAARLV PMGFVTAADF
160 170 180 190 200
HMRRSELICL TTGSKNLDTL LGGGVETGSI TELFGEFRTG KSQLCHTLAV
210 220 230 240 250
TCQIPLDIGG GEGKCLYIDT EGTFRPVRLV SIAQRFGLDP DDALNNVAYA
260 270 280 290 300
RAYNADHQLR LLDAAAQMMS ESRFSLIVVD SVMALYRTDF SGRGELSARQ
310 320 330 340 350
MHLAKFMRAL QRLADQFGVA VVVTNQVVAQ VDGGMAFNPD PKKPIGGNIM
360 370 380 390 400
AHSSTTRLGF KKGKGCQRLC KVVDSPCLPE AECVFAIYED GVGDPREEDE
Length:400
Mass (Da):42,963
Last modified:May 1, 1992 - v1
Checksum:i66561B60122A39A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64270 Genomic DNA. Translation: CAA45563.1.
M88470 Genomic DNA. Translation: AAA34948.1.
D10023 Genomic DNA. Translation: BAA00913.1.
U18839 Genomic DNA. Translation: AAB64650.1.
BK006939 Genomic DNA. Translation: DAA07757.1.
PIRiA44348.
RefSeqiNP_011021.3. NM_001178986.3.

Genome annotation databases

EnsemblFungiiYER095W; YER095W; YER095W.
GeneIDi856831.
KEGGisce:YER095W.

Similar proteinsi

Entry informationi

Entry nameiRAD51_YEAST
AccessioniPrimary (citable) accession number: P25454
Secondary accession number(s): D3DM03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 22, 2017
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names