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Protein

Meiotic recombination protein DMC1

Gene

DMC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for meiotic recombination, synaptonemal complex formation and cell cycle progression. Recruited to meiosis recombination hotspots by MEI5 and SAE3.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei223ssDNA or dsDNABy similarity1
Binding sitei226ssDNABy similarity1
Binding sitei229ssDNA or dsDNABy similarity1
Binding sitei235ssDNA or dsDNABy similarity1
Binding sitei305ssDNABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi121 – 128ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATP binding Source: UniProtKB
  • DNA-dependent ATPase activity Source: GO_Central
  • double-stranded DNA binding Source: SGD
  • four-way junction DNA binding Source: GO_Central
  • recombinase activity Source: SGD
  • single-stranded DNA binding Source: SGD

GO - Biological processi

  • DNA recombinase assembly Source: GO_Central
  • meiotic cell cycle Source: SGD
  • meiotic joint molecule formation Source: SGD
  • mitotic recombination Source: GO_Central
  • reciprocal meiotic recombination Source: SGD
  • response to ionizing radiation Source: GO_Central
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  • strand invasion Source: GO_Central
  • synaptonemal complex assembly Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, Meiosis, Sporulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30337-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic recombination protein DMC1
Gene namesi
Name:DMC1
Synonyms:ISC2
Ordered Locus Names:YER179W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER179W
SGDiS000000981 DMC1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi69K → E: Recessive mutant; phenotypically null. Eliminates the ability for self-association. 1 Publication1
Mutagenesisi126G → D: Dominant mutant; phenotypically null. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001229151 – 334Meiotic recombination protein DMC1Add BLAST334

Proteomic databases

PaxDbiP25453
PRIDEiP25453
TopDownProteomicsiP25453

Expressioni

Developmental stagei

Expressed 2.5 hours after induction of meiosis.

Interactioni

Subunit structurei

Double stacked ring-shaped homooctamer (By similarity). Forms a ternary complex with MEI5 and SAE3. Interacts with RDH54.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MEI5P324894EBI-5955,EBI-10685

Protein-protein interaction databases

BioGridi36932, 87 interactors
DIPiDIP-110N
IntActiP25453, 45 interactors
MINTiP25453
STRINGi4932.YER179W

Structurei

3D structure databases

ProteinModelPortaliP25453
SMRiP25453
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RecA family. DMC1 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00760000119398
HOGENOMiHOG000227426
InParanoidiP25453
KOiK10872
OMAiCTVKGIQ
OrthoDBiEOG092C3DRU

Family and domain databases

CDDicd01123 Rad51_DMC1_radA, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011940 DMC1_rcmbase
IPR013632 DNA_recomb/repair_Rad51_C
IPR016467 DNA_recomb/repair_RecA-like
IPR010995 DNA_repair_Rad51/TF_NusA_a-hlx
IPR027417 P-loop_NTPase
IPR033925 Rad51_DMC1_RadA
IPR020588 RecA_ATP-bd
IPR020587 RecA_monomer-monomer_interface
PfamiView protein in Pfam
PF08423 Rad51, 1 hit
PIRSFiPIRSF005856 Rad51, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF47794 SSF47794, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02238 recomb_DMC1, 1 hit
PROSITEiView protein in PROSITE
PS50162 RECA_2, 1 hit
PS50163 RECA_3, 1 hit

Sequencei

Sequence statusi: Complete.

P25453-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVTGTEIDS DTAKNILSVD ELQNYGINAS DLQKLKSGGI YTVNTVLSTT
60 70 80 90 100
RRHLCKIKGL SEVKVEKIKE AAGKIIQVGF IPATVQLDIR QRVYSLSTGS
110 120 130 140 150
KQLDSILGGG IMTMSITEVF GEFRCGKTQM SHTLCVTTQL PREMGGGEGK
160 170 180 190 200
VAYIDTEGTF RPERIKQIAE GYELDPESCL ANVSYARALN SEHQMELVEQ
210 220 230 240 250
LGEELSSGDY RLIVVDSIMA NFRVDYCGRG ELSERQQKLN QHLFKLNRLA
260 270 280 290 300
EEFNVAVFLT NQVQSDPGAS ALFASADGRK PIGGHVLAHA SATRILLRKG
310 320 330
RGDERVAKLQ DSPDMPEKEC VYVIGEKGIT DSSD
Length:334
Mass (Da):36,613
Last modified:May 1, 1992 - v1
Checksum:i00B0503D7FAF5CB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87549 Genomic DNA Translation: AAA34571.1
D10865 Genomic DNA Translation: BAA01637.1
U18922 Genomic DNA Translation: AAB64706.1
BK006939 Genomic DNA Translation: DAA07842.1
PIRiA38214
RefSeqiNP_011106.1, NM_001179069.1

Genome annotation databases

EnsemblFungiiYER179W; YER179W; YER179W
GeneIDi856926
KEGGisce:YER179W

Similar proteinsi

Entry informationi

Entry nameiDMC1_YEAST
AccessioniPrimary (citable) accession number: P25453
Secondary accession number(s): D3DM88
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health