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Protein

Meiotic recombination protein DMC1

Gene

DMC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for meiotic recombination, synaptonemal complex formation and cell cycle progression. Recruited to meiosis recombination hotspots by MEI5 and SAE3.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei223 – 2231ssDNA or dsDNABy similarity
Binding sitei226 – 2261ssDNABy similarity
Binding sitei229 – 2291ssDNA or dsDNABy similarity
Binding sitei235 – 2351ssDNA or dsDNABy similarity
Binding sitei305 – 3051ssDNABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi121 – 1288ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • DNA repair Source: InterPro
  • meiotic joint molecule formation Source: SGD
  • meiotic nuclear division Source: SGD
  • reciprocal meiotic recombination Source: SGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  • synaptonemal complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Meiosis, Sporulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30337-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic recombination protein DMC1
Gene namesi
Name:DMC1
Synonyms:ISC2
Ordered Locus Names:YER179W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER179W.
SGDiS000000981. DMC1.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi69 – 691K → E: Recessive mutant; phenotypically null. Eliminates the ability for self-association. 1 Publication
Mutagenesisi126 – 1261G → D: Dominant mutant; phenotypically null. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Meiotic recombination protein DMC1PRO_0000122915Add
BLAST

Proteomic databases

PeptideAtlasiP25453.
TopDownProteomicsiP25453.

Expressioni

Developmental stagei

Expressed 2.5 hours after induction of meiosis.

Interactioni

Subunit structurei

Double stacked ring-shaped homooctamer (By similarity). Forms a ternary complex with MEI5 and SAE3. Interacts with RDH54.By similarity3 Publications

Protein-protein interaction databases

BioGridi36932. 58 interactions.
DIPiDIP-110N.
IntActiP25453. 4 interactions.
MINTiMINT-387175.

Structurei

3D structure databases

ProteinModelPortaliP25453.
SMRiP25453. Positions 26-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RecA family. DMC1 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00760000119398.
HOGENOMiHOG000227426.
InParanoidiP25453.
KOiK10872.
OMAiRAYNSDM.
OrthoDBiEOG7TN02X.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011940. DMC1_rcmbase.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02238. recomb_DMC1. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25453-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVTGTEIDS DTAKNILSVD ELQNYGINAS DLQKLKSGGI YTVNTVLSTT
60 70 80 90 100
RRHLCKIKGL SEVKVEKIKE AAGKIIQVGF IPATVQLDIR QRVYSLSTGS
110 120 130 140 150
KQLDSILGGG IMTMSITEVF GEFRCGKTQM SHTLCVTTQL PREMGGGEGK
160 170 180 190 200
VAYIDTEGTF RPERIKQIAE GYELDPESCL ANVSYARALN SEHQMELVEQ
210 220 230 240 250
LGEELSSGDY RLIVVDSIMA NFRVDYCGRG ELSERQQKLN QHLFKLNRLA
260 270 280 290 300
EEFNVAVFLT NQVQSDPGAS ALFASADGRK PIGGHVLAHA SATRILLRKG
310 320 330
RGDERVAKLQ DSPDMPEKEC VYVIGEKGIT DSSD
Length:334
Mass (Da):36,613
Last modified:May 1, 1992 - v1
Checksum:i00B0503D7FAF5CB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87549 Genomic DNA. Translation: AAA34571.1.
D10865 Genomic DNA. Translation: BAA01637.1.
U18922 Genomic DNA. Translation: AAB64706.1.
BK006939 Genomic DNA. Translation: DAA07842.1.
PIRiA38214.
RefSeqiNP_011106.1. NM_001179069.1.

Genome annotation databases

EnsemblFungiiYER179W; YER179W; YER179W.
GeneIDi856926.
KEGGisce:YER179W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87549 Genomic DNA. Translation: AAA34571.1.
D10865 Genomic DNA. Translation: BAA01637.1.
U18922 Genomic DNA. Translation: AAB64706.1.
BK006939 Genomic DNA. Translation: DAA07842.1.
PIRiA38214.
RefSeqiNP_011106.1. NM_001179069.1.

3D structure databases

ProteinModelPortaliP25453.
SMRiP25453. Positions 26-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36932. 58 interactions.
DIPiDIP-110N.
IntActiP25453. 4 interactions.
MINTiMINT-387175.

Proteomic databases

PeptideAtlasiP25453.
TopDownProteomicsiP25453.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER179W; YER179W; YER179W.
GeneIDi856926.
KEGGisce:YER179W.

Organism-specific databases

EuPathDBiFungiDB:YER179W.
SGDiS000000981. DMC1.

Phylogenomic databases

GeneTreeiENSGT00760000119398.
HOGENOMiHOG000227426.
InParanoidiP25453.
KOiK10872.
OMAiRAYNSDM.
OrthoDBiEOG7TN02X.

Enzyme and pathway databases

BioCyciYEAST:G3O-30337-MONOMER.

Miscellaneous databases

PROiP25453.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011940. DMC1_rcmbase.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02238. recomb_DMC1. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression."
    Bishop D.K., Park D., Xu L., Kleckner N.
    Cell 69:439-456(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SK1.
  2. "Isolation and characterization of a yeast gene that is homologous with a meiosis-specific cDNA from a plant."
    Kobayashi T., Hotta Y., Tabata S.
    Mol. Gen. Genet. 237:225-232(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SK1.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway."
    Dresser M.E., Ewing D.J., Conrad M.N., Dominguez A.M., Barstead R., Jiang H., Kodadek T.
    Genetics 147:533-544(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RDH54, MUTAGENESIS OF LYS-69 AND GLY-126.
  6. "A protein complex containing Mei5 and Sae3 promotes the assembly of the meiosis-specific RecA homolog Dmc1."
    Hayase A., Takagi M., Miyazaki T., Oshiumi H., Shinohara M., Shinohara A.
    Cell 119:927-940(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MEI5 AND SAE3.
  7. "Molecular visualization of the yeast Dmc1 protein ring and Dmc1-ssDNA nucleoprotein complex."
    Chang Y.-C., Lo Y.-H., Lee M.-H., Leng C.-H., Hu S.-M., Chang C.-S., Wang T.-F.
    Biochemistry 44:6052-6058(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiDMC1_YEAST
AccessioniPrimary (citable) accession number: P25453
Secondary accession number(s): D3DM88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 8, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.