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P25443

- RS2_YEAST

UniProt

P25443 - RS2_YEAST

Protein

40S ribosomal protein S2

Gene

RPS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Important in the assembly and function of the 40S ribosomal subunit. Mutations in this protein affects the control of translational fidelity. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.1 Publication

    GO - Molecular functioni

    1. fibroblast growth factor binding Source: UniProtKB
    2. small ribosomal subunit rRNA binding Source: SGD
    3. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. positive regulation of translational fidelity Source: SGD
    2. rRNA export from nucleus Source: SGD
    3. rRNA processing Source: UniProtKB-KW
    4. translation Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30620-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    40S ribosomal protein S2
    Alternative name(s):
    Omnipotent suppressor protein SUP44
    RP12
    S4
    YS5
    Gene namesi
    Name:RPS2
    Synonyms:RPS4, SUP38, SUP44
    Ordered Locus Names:YGL123W
    ORF Names:G2893
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    SGDiS000003091. RPS2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosolic small ribosomal subunit Source: SGD
    2. nucleolus Source: UniProtKB-SubCell
    3. small-subunit processome Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed3 Publications
    Chaini2 – 25425340S ribosomal protein S2PRO_0000131684Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine3 Publications

    Post-translational modificationi

    N-terminally acetylated by acetyltransferase NatA.3 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP25443.
    PaxDbiP25443.
    PeptideAtlasiP25443.

    Expressioni

    Gene expression databases

    GenevestigatoriP25443.

    Interactioni

    Subunit structurei

    Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.2 Publications

    Protein-protein interaction databases

    BioGridi33128. 59 interactions.
    IntActiP25443. 11 interactions.
    MINTiMINT-4808257.
    STRINGi4932.YGL123W.

    Structurei

    Secondary structure

    1
    254
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi40 – 467
    Helixi53 – 586
    Helixi66 – 727
    Beta strandi80 – 9011
    Beta strandi93 – 10513
    Beta strandi107 – 12014
    Helixi121 – 13414
    Beta strandi144 – 1463
    Beta strandi153 – 1564
    Beta strandi158 – 1625
    Beta strandi165 – 1717
    Beta strandi178 – 1803
    Helixi182 – 1909
    Beta strandi196 – 2027
    Helixi207 – 21913
    Helixi220 – 2234
    Helixi227 – 2293
    Helixi239 – 2424
    Turni243 – 2464

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Xmodel-E75-223[»]
    1S1Helectron microscopy11.70E75-223[»]
    1VW9electron microscopy6.10D1-254[»]
    1VWVelectron microscopy6.10D1-254[»]
    3IZBelectron microscopy-E1-254[»]
    3O2ZX-ray4.00B1-254[»]
    3O30X-ray4.00B1-254[»]
    3U5CX-ray3.00C1-254[»]
    3U5GX-ray3.00C1-254[»]
    4BYLelectron microscopy4.30C1-254[»]
    4BYTelectron microscopy6.60C1-254[»]
    4CUYelectron microscopy3.70C34-249[»]
    ProteinModelPortaliP25443.
    SMRiP25443. Positions 34-250.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP25443.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini76 – 13964S5 DRBMPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ribosomal protein S5P family.Curated
    Contains 1 S5 DRBM domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0098.
    GeneTreeiENSGT00390000001433.
    HOGENOMiHOG000072596.
    KOiK02981.
    OMAiTHTRVAV.
    OrthoDBiEOG7C8GV0.

    Family and domain databases

    Gene3Di3.30.160.20. 1 hit.
    3.30.230.10. 1 hit.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR000851. Ribosomal_S5.
    IPR005324. Ribosomal_S5_C.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR005711. Ribosomal_S5_euk/arc.
    IPR013810. Ribosomal_S5_N.
    IPR018192. Ribosomal_S5_N_CS.
    [Graphical view]
    PANTHERiPTHR13718. PTHR13718. 1 hit.
    PfamiPF00333. Ribosomal_S5. 1 hit.
    PF03719. Ribosomal_S5_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 1 hit.
    TIGRFAMsiTIGR01020. rpsE_arch. 1 hit.
    PROSITEiPS00585. RIBOSOMAL_S5. 1 hit.
    PS50881. S5_DSRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25443-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAPEAQQQK RGGFGGRNRG RPNRRGPRNT EEKGWVPVTK LGRLVKAGKI    50
    TTIEEIFLHS LPVKEFQIID TLLPGLQDEV MNIKPVQKQT RAGQRTRFKA 100
    VVVVGDSNGH VGLGIKTAKE VAGAIRAGII IAKLSVIPIR RGYWGTNLGQ 150
    PHSLATKTTG KCGSVTVRLI PAPRGSGIVA SPAVKKLLQL AGVEDVYTQS 200
    NGKTRTLENT LKAAFVAIGN TYGFLTPNLW AEQPLPVSPL DIYSDEASAQ 250
    KKRF 254
    Length:254
    Mass (Da):27,450
    Last modified:January 23, 2007 - v3
    Checksum:i5EFDA4C7DE7BDFFB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59375 Genomic DNA. Translation: AAA63576.1.
    X94106 Genomic DNA. Translation: CAA63835.1.
    Z72645 Genomic DNA. Translation: CAA96831.1.
    AY557815 Genomic DNA. Translation: AAS56141.1.
    BK006941 Genomic DNA. Translation: DAA07986.1.
    PIRiA36363. R3BYS2.
    RefSeqiNP_011392.1. NM_001180988.1.

    Genome annotation databases

    EnsemblFungiiYGL123W; YGL123W; YGL123W.
    GeneIDi852754.
    KEGGisce:YGL123W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59375 Genomic DNA. Translation: AAA63576.1 .
    X94106 Genomic DNA. Translation: CAA63835.1 .
    Z72645 Genomic DNA. Translation: CAA96831.1 .
    AY557815 Genomic DNA. Translation: AAS56141.1 .
    BK006941 Genomic DNA. Translation: DAA07986.1 .
    PIRi A36363. R3BYS2.
    RefSeqi NP_011392.1. NM_001180988.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5X model - E 75-223 [» ]
    1S1H electron microscopy 11.70 E 75-223 [» ]
    1VW9 electron microscopy 6.10 D 1-254 [» ]
    1VWV electron microscopy 6.10 D 1-254 [» ]
    3IZB electron microscopy - E 1-254 [» ]
    3O2Z X-ray 4.00 B 1-254 [» ]
    3O30 X-ray 4.00 B 1-254 [» ]
    3U5C X-ray 3.00 C 1-254 [» ]
    3U5G X-ray 3.00 C 1-254 [» ]
    4BYL electron microscopy 4.30 C 1-254 [» ]
    4BYT electron microscopy 6.60 C 1-254 [» ]
    4CUY electron microscopy 3.70 C 34-249 [» ]
    ProteinModelPortali P25443.
    SMRi P25443. Positions 34-250.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33128. 59 interactions.
    IntActi P25443. 11 interactions.
    MINTi MINT-4808257.
    STRINGi 4932.YGL123W.

    Proteomic databases

    MaxQBi P25443.
    PaxDbi P25443.
    PeptideAtlasi P25443.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL123W ; YGL123W ; YGL123W .
    GeneIDi 852754.
    KEGGi sce:YGL123W.

    Organism-specific databases

    SGDi S000003091. RPS2.

    Phylogenomic databases

    eggNOGi COG0098.
    GeneTreei ENSGT00390000001433.
    HOGENOMi HOG000072596.
    KOi K02981.
    OMAi THTRVAV.
    OrthoDBi EOG7C8GV0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30620-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    EvolutionaryTracei P25443.
    NextBioi 972192.
    PROi P25443.

    Gene expression databases

    Genevestigatori P25443.

    Family and domain databases

    Gene3Di 3.30.160.20. 1 hit.
    3.30.230.10. 1 hit.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR000851. Ribosomal_S5.
    IPR005324. Ribosomal_S5_C.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR005711. Ribosomal_S5_euk/arc.
    IPR013810. Ribosomal_S5_N.
    IPR018192. Ribosomal_S5_N_CS.
    [Graphical view ]
    PANTHERi PTHR13718. PTHR13718. 1 hit.
    Pfami PF00333. Ribosomal_S5. 1 hit.
    PF03719. Ribosomal_S5_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54211. SSF54211. 1 hit.
    TIGRFAMsi TIGR01020. rpsE_arch. 1 hit.
    PROSITEi PS00585. RIBOSOMAL_S5. 1 hit.
    PS50881. S5_DSRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and functional similarity between a yeast ribosomal protein and the Escherichia coli S5 ram protein."
      All-Robyn J.A., Brown N., Otaka E., Liebman S.W.
      Mol. Cell. Biol. 10:6544-6553(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification of a putative methylenetetrahydrofolate reductase by sequence analysis of a 6.8 kb DNA fragment of yeast chromosome VII."
      Tizon B., Rodriguez-Torres A.M., Rodriguez-Belmonte E., Cadahia J.L., Cerdan E.
      Yeast 12:1047-1051(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
      Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
      J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-11, ACETYLATION AT SER-2.
    7. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    8. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
    9. "The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes."
      Schaefer T., Strauss D., Petfalski E., Tollervey D., Hurt E.
      EMBO J. 22:1370-1380(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    10. "The small-subunit processome is a ribosome assembly intermediate."
      Bernstein K.A., Gallagher J.E.G., Mitchell B.M., Granneman S., Baserga S.J.
      Eukaryot. Cell 3:1619-1626(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME, SUBCELLULAR LOCATION.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 75-223.
    15. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 75-223, ELECTRON MICROSCOPY.

    Entry informationi

    Entry nameiRS2_YEAST
    AccessioniPrimary (citable) accession number: P25443
    Secondary accession number(s): D6VU25
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 134 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3