40S ribosomal protein S2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P25443 (RS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 122. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
40S ribosomal protein S2

Alternative name(s):
Omnipotent suppressor protein SUP44
RP12
S4
YS5

Gene names

Name:	RPS2
Synonyms:	RPS4, SUP38, SUP44
Ordered Locus Names:	YGL123W
ORF Names:	G2893

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Important in the assembly and function of the 40S ribosomal subunit. Mutations in this protein affects the control of translational fidelity. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Ref.10
Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.7 Ref.10
Subcellular location	Cytoplasm. Nucleus › nucleolus Ref.9 Ref.10.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA.
Sequence similarities	Belongs to the ribosomal protein S5P family. Contains 1 S5 DRBM domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm Nucleus
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of translational fidelity Inferred from mutant phenotype PubMed 17545469 PubMed 3522920. Source: SGD rRNA export from nucleus Inferred from mutant phenotype PubMed 16246728. Source: SGD rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW translation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosolic small ribosomal subunit Inferred from direct assay PubMed 2517480 PubMed 3533916 PubMed 385049. Source: SGD nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell small-subunit processome Inferred from direct assay Ref.10. Source: SGD
Molecular_function	SSU rRNA binding Inferred from direct assay PubMed 2517480. Source: SGD structural constituent of ribosome Inferred from direct assay PubMed 2517480. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.6 Ref.8

Chain

2 – 254

253

40S ribosomal protein S2

PRO_0000131684

Regions

Domain

76 – 139

S5 DRBM

Amino acid modifications

Modified residue

N-acetylserine Ref.6 Ref.8

Modified residue

181

Phosphoserine Ref.11

Modified residue

244

Phosphoserine Ref.11

Secondary structure

254

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P25443 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 5EFDA4C7DE7BDFFB
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FASTA

254

27,450

        10         20         30         40         50         60 
MSAPEAQQQK RGGFGGRNRG RPNRRGPRNT EEKGWVPVTK LGRLVKAGKI TTIEEIFLHS 

        70         80         90        100        110        120 
LPVKEFQIID TLLPGLQDEV MNIKPVQKQT RAGQRTRFKA VVVVGDSNGH VGLGIKTAKE 

       130        140        150        160        170        180 
VAGAIRAGII IAKLSVIPIR RGYWGTNLGQ PHSLATKTTG KCGSVTVRLI PAPRGSGIVA 

       190        200        210        220        230        240 
SPAVKKLLQL AGVEDVYTQS NGKTRTLENT LKAAFVAIGN TYGFLTPNLW AEQPLPVSPL 

       250 
DIYSDEASAQ KKRF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and functional similarity between a yeast ribosomal protein and the Escherichia coli S5 ram protein." All-Robyn J.A., Brown N., Otaka E., Liebman S.W. Mol. Cell. Biol. 10:6544-6553(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification of a putative methylenetetrahydrofolate reductase by sequence analysis of a 6.8 kb DNA fragment of yeast chromosome VII." Tizon B., Rodriguez-Torres A.M., Rodriguez-Belmonte E., Cadahia J.L., Cerdan E. Yeast 12:1047-1051(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11, ACETYLATION AT SER-2.
[7]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[8]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[9]	"The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes." Schaefer T., Strauss D., Petfalski E., Tollervey D., Hurt E. EMBO J. 22:1370-1380(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[10]	"The small-subunit processome is a ribosome assembly intermediate." Bernstein K.A., Gallagher J.E.G., Mitchell B.M., Granneman S., Baserga S.J. Eukaryot. Cell 3:1619-1626(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME, SUBCELLULAR LOCATION.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181 AND SER-244, MASS SPECTROMETRY.
[12]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 75-223.
[13]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 75-223, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M59375 Genomic DNA. Translation: AAA63576.1.
X94106 Genomic DNA. Translation: CAA63835.1.
Z72645 Genomic DNA. Translation: CAA96831.1.
AY557815 Genomic DNA. Translation: AAS56141.1.
BK006941 Genomic DNA. Translation: DAA07986.1.

PIR

R3BYS2. A36363.

RefSeq

NP_011392.1. NM_001180988.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5X	model	-	E	75-223	[»]
1S1H	electron microscopy	11.70	E	75-223	[»]
3IZB	electron microscopy	-	E	1-254	[»]
3O2Z	X-ray	4.00	B	1-254	[»]
3O30	X-ray	4.00	B	1-254	[»]
3U5C	X-ray	3.00	C	1-254	[»]
3U5G	X-ray	3.00	C	1-254	[»]

ProteinModelPortal

P25443.

SMR

P25443. Positions 34-250.

ModBase

Search...

Protein-protein interaction databases

IntAct

P25443. 8 interactions.

STRING

4932.YGL123W.

Proteomic databases

PaxDb

P25443.

PeptideAtlas

P25443.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL123W; YGL123W; YGL123W.

GeneID

852754.

KEGG

sce:YGL123W.

Organism-specific databases

SGD

S000003091. RPS2.

Phylogenomic databases

eggNOG

COG0098.

GeneTree

ENSGT00390000001433.

HOGENOM

HOG000072596.

K02981.

OMA

FSMAPFQ.

OrthoDB

EOG4T1MWN.

Gene expression databases

Genevestigator

P25443.

GermOnline

YGL123W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.30.160.20. 1 hit.
3.30.230.10. 1 hit.

InterPro

IPR014720. dsRNA-bd-like_dom.
IPR000851. Ribosomal_S5.
IPR005324. Ribosomal_S5_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005711. Ribosomal_S5_euk/arc.
IPR013810. Ribosomal_S5_N.
IPR018192. Ribosomal_S5_N_CS.
[Graphical view]

PANTHER

PTHR13718. PTHR13718. 1 hit.

Pfam

PF00333. Ribosomal_S5. 1 hit.
PF03719. Ribosomal_S5_C. 1 hit.
[Graphical view]

SUPFAM

SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.

TIGRFAMs

TIGR01020. rpsE_arch. 1 hit.

PROSITE

PS00585. RIBOSOMAL_S5. 1 hit.
PS50881. S5_DSRBD. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P25443.

NextBio

972192.

Entry information

Entry name

RS2_YEAST

Accession

Primary (citable) accession number: P25443
Secondary accession number(s): D6VU25

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 122 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents