Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P25443

- RS2_YEAST

UniProt

P25443 - RS2_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

40S ribosomal protein S2

Gene

RPS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Important in the assembly and function of the 40S ribosomal subunit. Mutations in this protein affects the control of translational fidelity. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.1 Publication

GO - Molecular functioni

  1. fibroblast growth factor binding Source: UniProtKB
  2. small ribosomal subunit rRNA binding Source: SGD
  3. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. positive regulation of translational fidelity Source: SGD
  2. rRNA export from nucleus Source: SGD
  3. rRNA processing Source: UniProtKB-KW
  4. translation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-30620-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S2
Alternative name(s):
Omnipotent suppressor protein SUP44
RP12
S4
YS5
Gene namesi
Name:RPS2
Synonyms:RPS4, SUP38, SUP44
Ordered Locus Names:YGL123W
ORF Names:G2893
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

SGDiS000003091. RPS2.

Subcellular locationi

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: SGD
  2. nucleus Source: UniProtKB-KW
  3. small-subunit processome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed3 Publications
Chaini2 – 25425340S ribosomal protein S2PRO_0000131684Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine3 Publications
Modified residuei11 – 111Asymmetric dimethylarginine; by HMT1; alternate2 Publications
Modified residuei11 – 111Omega-N-methylarginine; by HMT1; alternate2 Publications

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.3 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP25443.
PaxDbiP25443.
PeptideAtlasiP25443.

Expressioni

Gene expression databases

GenevestigatoriP25443.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.2 Publications

Protein-protein interaction databases

BioGridi33128. 60 interactions.
IntActiP25443. 11 interactions.
MINTiMINT-4808257.
STRINGi4932.YGL123W.

Structurei

Secondary structure

1
254
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi40 – 467
Helixi53 – 586
Helixi66 – 727
Beta strandi80 – 9011
Beta strandi93 – 10513
Beta strandi107 – 12014
Helixi121 – 13414
Beta strandi144 – 1463
Beta strandi153 – 1564
Beta strandi158 – 1625
Beta strandi165 – 1717
Beta strandi178 – 1803
Helixi182 – 1909
Beta strandi196 – 2027
Helixi207 – 21913
Helixi220 – 2234
Helixi227 – 2293
Helixi239 – 2424
Turni243 – 2464

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Xmodel-E75-223[»]
1S1Helectron microscopy11.70E75-223[»]
1VW9electron microscopy6.10D1-254[»]
1VWVelectron microscopy6.10D1-254[»]
1VXWelectron microscopy6.20C1-254[»]
1VXZelectron microscopy6.30C1-254[»]
3IZBelectron microscopy-E1-254[»]
3O2ZX-ray4.00B1-254[»]
3O30X-ray4.00B1-254[»]
3U5CX-ray3.00C1-254[»]
3U5GX-ray3.00C1-254[»]
4BYLelectron microscopy4.30C1-254[»]
4BYTelectron microscopy6.60C1-254[»]
4CUYelectron microscopy3.70C34-249[»]
ProteinModelPortaliP25443.
SMRiP25443. Positions 34-250.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25443.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini76 – 13964S5 DRBMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ribosomal protein S5P family.Curated
Contains 1 S5 DRBM domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0098.
GeneTreeiENSGT00390000001433.
HOGENOMiHOG000072596.
InParanoidiP25443.
KOiK02981.
OMAiTHTRVAV.
OrthoDBiEOG7C8GV0.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
3.30.230.10. 1 hit.
InterProiIPR014720. dsRNA-bd_dom.
IPR000851. Ribosomal_S5.
IPR005324. Ribosomal_S5_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005711. Ribosomal_S5_euk/arc.
IPR013810. Ribosomal_S5_N.
IPR018192. Ribosomal_S5_N_CS.
[Graphical view]
PANTHERiPTHR13718. PTHR13718. 1 hit.
PfamiPF00333. Ribosomal_S5. 1 hit.
PF03719. Ribosomal_S5_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
TIGRFAMsiTIGR01020. rpsE_arch. 1 hit.
PROSITEiPS00585. RIBOSOMAL_S5. 1 hit.
PS50881. S5_DSRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25443-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAPEAQQQK RGGFGGRNRG RPNRRGPRNT EEKGWVPVTK LGRLVKAGKI
60 70 80 90 100
TTIEEIFLHS LPVKEFQIID TLLPGLQDEV MNIKPVQKQT RAGQRTRFKA
110 120 130 140 150
VVVVGDSNGH VGLGIKTAKE VAGAIRAGII IAKLSVIPIR RGYWGTNLGQ
160 170 180 190 200
PHSLATKTTG KCGSVTVRLI PAPRGSGIVA SPAVKKLLQL AGVEDVYTQS
210 220 230 240 250
NGKTRTLENT LKAAFVAIGN TYGFLTPNLW AEQPLPVSPL DIYSDEASAQ

KKRF
Length:254
Mass (Da):27,450
Last modified:January 23, 2007 - v3
Checksum:i5EFDA4C7DE7BDFFB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59375 Genomic DNA. Translation: AAA63576.1.
X94106 Genomic DNA. Translation: CAA63835.1.
Z72645 Genomic DNA. Translation: CAA96831.1.
AY557815 Genomic DNA. Translation: AAS56141.1.
BK006941 Genomic DNA. Translation: DAA07986.1.
PIRiA36363. R3BYS2.
RefSeqiNP_011392.1. NM_001180988.1.

Genome annotation databases

EnsemblFungiiYGL123W; YGL123W; YGL123W.
GeneIDi852754.
KEGGisce:YGL123W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59375 Genomic DNA. Translation: AAA63576.1 .
X94106 Genomic DNA. Translation: CAA63835.1 .
Z72645 Genomic DNA. Translation: CAA96831.1 .
AY557815 Genomic DNA. Translation: AAS56141.1 .
BK006941 Genomic DNA. Translation: DAA07986.1 .
PIRi A36363. R3BYS2.
RefSeqi NP_011392.1. NM_001180988.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K5X model - E 75-223 [» ]
1S1H electron microscopy 11.70 E 75-223 [» ]
1VW9 electron microscopy 6.10 D 1-254 [» ]
1VWV electron microscopy 6.10 D 1-254 [» ]
1VXW electron microscopy 6.20 C 1-254 [» ]
1VXZ electron microscopy 6.30 C 1-254 [» ]
3IZB electron microscopy - E 1-254 [» ]
3O2Z X-ray 4.00 B 1-254 [» ]
3O30 X-ray 4.00 B 1-254 [» ]
3U5C X-ray 3.00 C 1-254 [» ]
3U5G X-ray 3.00 C 1-254 [» ]
4BYL electron microscopy 4.30 C 1-254 [» ]
4BYT electron microscopy 6.60 C 1-254 [» ]
4CUY electron microscopy 3.70 C 34-249 [» ]
ProteinModelPortali P25443.
SMRi P25443. Positions 34-250.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33128. 60 interactions.
IntActi P25443. 11 interactions.
MINTi MINT-4808257.
STRINGi 4932.YGL123W.

Proteomic databases

MaxQBi P25443.
PaxDbi P25443.
PeptideAtlasi P25443.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL123W ; YGL123W ; YGL123W .
GeneIDi 852754.
KEGGi sce:YGL123W.

Organism-specific databases

SGDi S000003091. RPS2.

Phylogenomic databases

eggNOGi COG0098.
GeneTreei ENSGT00390000001433.
HOGENOMi HOG000072596.
InParanoidi P25443.
KOi K02981.
OMAi THTRVAV.
OrthoDBi EOG7C8GV0.

Enzyme and pathway databases

BioCyci YEAST:G3O-30620-MONOMER.
Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Miscellaneous databases

EvolutionaryTracei P25443.
NextBioi 972192.
PROi P25443.

Gene expression databases

Genevestigatori P25443.

Family and domain databases

Gene3Di 3.30.160.20. 1 hit.
3.30.230.10. 1 hit.
InterProi IPR014720. dsRNA-bd_dom.
IPR000851. Ribosomal_S5.
IPR005324. Ribosomal_S5_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005711. Ribosomal_S5_euk/arc.
IPR013810. Ribosomal_S5_N.
IPR018192. Ribosomal_S5_N_CS.
[Graphical view ]
PANTHERi PTHR13718. PTHR13718. 1 hit.
Pfami PF00333. Ribosomal_S5. 1 hit.
PF03719. Ribosomal_S5_C. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
TIGRFAMsi TIGR01020. rpsE_arch. 1 hit.
PROSITEi PS00585. RIBOSOMAL_S5. 1 hit.
PS50881. S5_DSRBD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and functional similarity between a yeast ribosomal protein and the Escherichia coli S5 ram protein."
    All-Robyn J.A., Brown N., Otaka E., Liebman S.W.
    Mol. Cell. Biol. 10:6544-6553(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of a putative methylenetetrahydrofolate reductase by sequence analysis of a 6.8 kb DNA fragment of yeast chromosome VII."
    Tizon B., Rodriguez-Torres A.M., Rodriguez-Belmonte E., Cadahia J.L., Cerdan E.
    Yeast 12:1047-1051(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
    Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
    J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-11, ACETYLATION AT SER-2.
  7. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  8. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
  9. "The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes."
    Schaefer T., Strauss D., Petfalski E., Tollervey D., Hurt E.
    EMBO J. 22:1370-1380(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. "The small-subunit processome is a ribosome assembly intermediate."
    Bernstein K.A., Gallagher J.E.G., Mitchell B.M., Granneman S., Baserga S.J.
    Eukaryot. Cell 3:1619-1626(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME, SUBCELLULAR LOCATION.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Rmt1 catalyzes zinc-finger independent arginine methylation of ribosomal protein Rps2 in Saccharomyces cerevisiae."
    Lipson R.S., Webb K.J., Clarke S.G.
    Biochem. Biophys. Res. Commun. 391:1658-1662(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION BY HMT1.
  13. "Identification of methylated proteins in the yeast small ribosomal subunit: a role for SPOUT methyltransferases in protein arginine methylation."
    Young B.D., Weiss D.I., Zurita-Lopez C.I., Webb K.J., Clarke S.G., McBride A.E.
    Biochemistry 51:5091-5104(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT ARG-11.
  14. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
    Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
    Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 75-223.
  17. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
    Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
    EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 75-223, ELECTRON MICROSCOPY.
  18. Cited for: STRUCTURE BY ELECTRON MICROSCOPY.
  19. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.00 ANGSTROMS) OF 1-254.
  20. Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 1-254.
  21. "Molecular architecture of a eukaryotic translational initiation complex."
    Fernandez I.S., Bai X.C., Hussain T., Kelley A.C., Lorsch J.R., Ramakrishnan V., Scheres S.H.
    Science 342:1240585-1240585(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (4.30 ANGSTROMS) OF 1-254.

Entry informationi

Entry nameiRS2_YEAST
AccessioniPrimary (citable) accession number: P25443
Secondary accession number(s): D6VU25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3