Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent helicase brm

Gene

brm

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator. Acts as a coactivator, assisting one or more dedicated transcriptional activators of ANTC and BXC homeotic gene clusters. Can counteract the repressive effect of Polycomb protein. ATPase subunit of the Brahma complex, a multiprotein complex which is the equivalent of the yeast SWI/SNF complex and acts by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. This complex can both serve as a transcriptional coactivator or corepressor, depending on the context.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi798 – 805ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: FlyBase
  • ATP binding Source: UniProtKB-KW
  • DNA-dependent ATPase activity Source: FlyBase
  • helicase activity Source: UniProtKB-KW

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: FlyBase
  • axonogenesis Source: FlyBase
  • dendrite guidance Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • hemocyte proliferation Source: FlyBase
  • hippo signaling Source: FlyBase
  • histone H3-K27 acetylation Source: FlyBase
  • imaginal disc-derived leg morphogenesis Source: FlyBase
  • imaginal disc-derived wing margin morphogenesis Source: FlyBase
  • imaginal disc-derived wing vein morphogenesis Source: FlyBase
  • imaginal disc-derived wing vein specification Source: FlyBase
  • intestinal stem cell homeostasis Source: FlyBase
  • muscle organ development Source: FlyBase
  • negative regulation of G1/S transition of mitotic cell cycle Source: FlyBase
  • negative regulation of neuroblast proliferation Source: FlyBase
  • neurogenesis Source: FlyBase
  • neuron development Source: FlyBase
  • oogenesis Source: FlyBase
  • phagocytosis Source: FlyBase
  • positive regulation of epidermal growth factor receptor signaling pathway Source: FlyBase
  • positive regulation of stem cell proliferation Source: FlyBase
  • positive regulation of transcription, DNA-templated Source: FlyBase
  • regulation of innate immune response Source: FlyBase
  • regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
  • ventral cord development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Developmental protein, Helicase, Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-1266695. Interleukin-7 signaling.
R-DME-3247509. Chromatin modifying enzymes.
SignaLinkiP25439.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase brm (EC:3.6.4.12)
Alternative name(s):
Homeotic gene regulator
Protein brahma
Gene namesi
Name:brm
ORF Names:CG5942
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0000212. brm.

Subcellular locationi

GO - Cellular componenti

  • brahma complex Source: FlyBase
  • nucleus Source: FlyBase
  • PBAF complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743101 – 1638ATP-dependent helicase brmAdd BLAST1638

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei695Phosphoserine1 Publication1
Modified residuei698Phosphoserine1 Publication1
Modified residuei1407Phosphoserine1 Publication1
Modified residuei1411Phosphoserine1 Publication1
Modified residuei1591Phosphoserine1 Publication1
Modified residuei1594Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP25439.
PRIDEiP25439.

PTM databases

iPTMnetiP25439.

Expressioni

Developmental stagei

Highest expression in unfertilized eggs and early embryos.

Gene expression databases

BgeeiFBgn0000212.
ExpressionAtlasiP25439. baseline.
GenevisibleiP25439. DM.

Interactioni

Subunit structurei

Component of the Brahma complex, which is composed of brm, osa, mor, Snr1/Bap45, dalao/Bap111, Bap55, Bap60 and Act42A/Bap47. Interacts with pnr and Chi via its EHD domain. Interacts with asf1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
morQ7KPY33EBI-868480,EBI-1182199
osaQ8IN944EBI-868480,EBI-115993
Snr1Q240904EBI-868480,EBI-151570

Protein-protein interaction databases

BioGridi65055. 59 interactors.
DIPiDIP-36728N.
IntActiP25439. 15 interactors.
MINTiMINT-6541230.
STRINGi7227.FBpp0303193.

Structurei

3D structure databases

ProteinModelPortaliP25439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini173 – 208QLQPROSITE-ProRule annotationAdd BLAST36
Domaini501 – 573HSAPROSITE-ProRule annotationAdd BLAST73
Domaini785 – 950Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST166
Domaini1102 – 1263Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162
Domaini1443 – 1513BromoPROSITE-ProRule annotationAdd BLAST71

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi900 – 903DEGH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi201 – 390Gln/Pro-richAdd BLAST190
Compositional biasi1385 – 1392Asp/Glu-rich (acidic)8
Compositional biasi1394 – 1404Arg/Lys-rich (basic)Add BLAST11
Compositional biasi1405 – 1410Asp/Glu-rich (acidic)6
Compositional biasi1415 – 1432Arg/Lys-rich (basic)Add BLAST18
Compositional biasi1631 – 1638Poly-Asp8

Sequence similaritiesi

Contains 1 bromo domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 HSA domain.PROSITE-ProRule annotation
Contains 1 QLQ domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain

Phylogenomic databases

eggNOGiKOG0386. Eukaryota.
COG0553. LUCA.
GeneTreeiENSGT00550000074659.
InParanoidiP25439.
KOiK11647.
OrthoDBiEOG091G01R9.
PhylomeDBiP25439.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR006576. BRK_domain.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR014978. Gln-Leu-Gln_QLQ.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014012. HSA_dom.
IPR027417. P-loop_NTPase.
IPR029295. SnAC.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF07533. BRK. 1 hit.
PF00439. Bromodomain. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07529. HSA. 1 hit.
PF08880. QLQ. 1 hit.
PF14619. SnAC. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00592. BRK. 1 hit.
SM00297. BROMO. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00573. HSA. 1 hit.
SM00951. QLQ. 1 hit.
SM01314. SnAC. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51204. HSA. 1 hit.
PS51666. QLQ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P25439-1) [UniParc]FASTAAdd to basket
Also known as: B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPSPANSP MPPPQAPSPM APPSQSPAPS PHSPYPHQQP GPLQGPPPPG
60 70 80 90 100
HPGAYGHPMQ HGPPGQGPPG HHMPPHHQGM IFSKGPHMGM QMPPTGPNMS
110 120 130 140 150
PYQTHGMPPN APTQPCIVSP GGPPGGPPPP ERSSQENLHA LQRAIDSMEE
160 170 180 190 200
KGLQEDPRYS QLLAMRATSK HQHLNGNQVN LLRTQITAYR LLARNKPISM
210 220 230 240 250
QMQQALQAAQ QQPPPGPPIG PPGAPGGPPP GSQHAGQPPV PPQQQQQPPP
260 270 280 290 300
SAGTPPQCST PPASNPYGPP VPGQKMQVAP PPPHMQQGQP LPPQPPQVGG
310 320 330 340 350
PPPIQQQQPP QQQQQQSQPP PPEPHQHQLP NGGKPLSMGP SGGQPLIPSS
360 370 380 390 400
PMQPQVRGTL PGMPPGSQVP QPGGGPQRQV PPAGMPMPKP NRITTVAKPV
410 420 430 440 450
GLDPITLLQE RENRIAARIS LRMQELQRLP ATMSEDLRLQ AAIELRALRV
460 470 480 490 500
LNFQRQLRME FVQCTRRDTT LETALNIKLY KRTKRQGLRE ARATEKLEKQ
510 520 530 540 550
QKLEAERKRR QKHLEFLAAV LQHGKDLREF HRNNKAQLAR MNKAVMNHHA
560 570 580 590 600
NAEREQKKEQ ERIEKERMRR LMAEDEEGYR KLIDQKKDKR LAFLLSQTDE
610 620 630 640 650
YISNLTQMVK QHKDDQMKKK EEEGKRLIQF KKELLMSGEY IGIDEGSIVA
660 670 680 690 700
DMRVHVVEQC TGKKLTGDDA PMLKHLHRWL NMHPGWDWID DEEDSCGSND
710 720 730 740 750
DHKPKVEEQP TATEDATDKA QATGNDEDAK DLITKAKVED DEYRTEEQTY
760 770 780 790 800
YSIAHTIHEK VVEQASIMVN GTLKEYQIKG LEWLVSLYNN NLNGILADEM
810 820 830 840 850
GLGKTIQTIS LVTYLMDRKK VMGPYLIIVP LSTLPNWVLE FEKWAPAVGV
860 870 880 890 900
VSYKGSPQGR RLLQNQMRAT KFNVLLTTYE YVIKDKAVLA KIQWKYMIID
910 920 930 940 950
EGHRMKNHHC KLTQVLNTHY IAPYRLLLTG TPLQNKLPEL WALLNFLLPS
960 970 980 990 1000
IFKSCSTFEQ WFNAPFATTG EKVELNEEET ILIIRRLHKV LRPFLLRRLK
1010 1020 1030 1040 1050
KEVEHQLPDK VEYIIKCDMS ALQRVLYKHM QSKGVLLTDG SEKGKHGKGG
1060 1070 1080 1090 1100
AKALMNTIVQ LRKLCNHPFM FQHIEEKYCD HTGGHGVVSG PDLYRVSGKF
1110 1120 1130 1140 1150
ELLDRILPKL KATNHRVLLF CQMTQCMTII EDYLGWRQFG YLRLDGTTKA
1160 1170 1180 1190 1200
EDRGELLRKF NAKGSDVFVF LLSTRAGGLG LNLQTADTVV IFDSDWNPHQ
1210 1220 1230 1240 1250
DLQAQDRAHR IGQRNEVRVL RLMTVNSVEE RILAAARYKL NMDEKVIQAG
1260 1270 1280 1290 1300
MFDQKSTGSE RQQFLQTILH QDDNEEEEEN EVPDDEMINM MIARSEEEIE
1310 1320 1330 1340 1350
IFKRMDAERK KEDEEIHPGR ERLIDESELP DWLTKDDDEV ERFHYQYDED
1360 1370 1380 1390 1400
TILGRGSRQR KEVDYTDSLT EKEWLKAIDD GAEFDEEEEE DDSKRKRRKR
1410 1420 1430 1440 1450
KNRKEESDDD SLILKRRRRQ NLDKRSKKQM HKIMSAVIKH NQDGRTLSEP
1460 1470 1480 1490 1500
FMKLPSRQRL PDYYEIIKRP VDIKKILQRI EDCKYADLNE LEKDFMQLCQ
1510 1520 1530 1540 1550
NAQIYNEEAS LIYLDSIALQ KVFVGARQRI TAAADAAAVA AGDNTGEAHG
1560 1570 1580 1590 1600
NGGSDNSDND DDDGGDDGSD DEEIATTSAA AVKMKLKLNK SLASAPATPT
1610 1620 1630
QSSSNVSSGA ATTSKKQTRR KRSQKKYTIS DDDDDDMD
Length:1,638
Mass (Da):185,089
Last modified:October 18, 2001 - v2
Checksum:iA4494B29F4F2E42A
GO
Isoform C (identifier: P25439-2) [UniParc]FASTAAdd to basket
Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
     81-85: IFSKG → S

Note: No experimental confirmation available.
Show »
Length:1,634
Mass (Da):184,644
Checksum:i07564D953CE5E88A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti687D → Y in AAA19661 (PubMed:1346755).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00055581 – 85IFSKG → S in isoform C. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85049 mRNA. Translation: AAA19661.1.
AE014296 Genomic DNA. Translation: AAF49557.1.
AE014296 Genomic DNA. Translation: AAF49558.3.
AE014296 Genomic DNA. Translation: AAN11773.1.
AE014296 Genomic DNA. Translation: AAN11774.1.
AY095048 mRNA. Translation: AAM11376.1.
BT009972 mRNA. Translation: AAQ22441.1.
PIRiA42091.
RefSeqiNP_536745.4. NM_080497.5. [P25439-2]
NP_536746.1. NM_080498.3. [P25439-1]
NP_730088.1. NM_168640.2. [P25439-2]
NP_730089.1. NM_168641.1. [P25439-1]
UniGeneiDm.5356.

Genome annotation databases

EnsemblMetazoaiFBtr0075525; FBpp0075280; FBgn0000212. [P25439-1]
FBtr0075526; FBpp0075281; FBgn0000212. [P25439-1]
GeneIDi39744.
KEGGidme:Dmel_CG5942.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85049 mRNA. Translation: AAA19661.1.
AE014296 Genomic DNA. Translation: AAF49557.1.
AE014296 Genomic DNA. Translation: AAF49558.3.
AE014296 Genomic DNA. Translation: AAN11773.1.
AE014296 Genomic DNA. Translation: AAN11774.1.
AY095048 mRNA. Translation: AAM11376.1.
BT009972 mRNA. Translation: AAQ22441.1.
PIRiA42091.
RefSeqiNP_536745.4. NM_080497.5. [P25439-2]
NP_536746.1. NM_080498.3. [P25439-1]
NP_730088.1. NM_168640.2. [P25439-2]
NP_730089.1. NM_168641.1. [P25439-1]
UniGeneiDm.5356.

3D structure databases

ProteinModelPortaliP25439.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi65055. 59 interactors.
DIPiDIP-36728N.
IntActiP25439. 15 interactors.
MINTiMINT-6541230.
STRINGi7227.FBpp0303193.

PTM databases

iPTMnetiP25439.

Proteomic databases

PaxDbiP25439.
PRIDEiP25439.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075525; FBpp0075280; FBgn0000212. [P25439-1]
FBtr0075526; FBpp0075281; FBgn0000212. [P25439-1]
GeneIDi39744.
KEGGidme:Dmel_CG5942.

Organism-specific databases

CTDi39744.
FlyBaseiFBgn0000212. brm.

Phylogenomic databases

eggNOGiKOG0386. Eukaryota.
COG0553. LUCA.
GeneTreeiENSGT00550000074659.
InParanoidiP25439.
KOiK11647.
OrthoDBiEOG091G01R9.
PhylomeDBiP25439.

Enzyme and pathway databases

ReactomeiR-DME-1266695. Interleukin-7 signaling.
R-DME-3247509. Chromatin modifying enzymes.
SignaLinkiP25439.

Miscellaneous databases

GenomeRNAii39744.
PROiP25439.

Gene expression databases

BgeeiFBgn0000212.
ExpressionAtlasiP25439. baseline.
GenevisibleiP25439. DM.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR006576. BRK_domain.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR014978. Gln-Leu-Gln_QLQ.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014012. HSA_dom.
IPR027417. P-loop_NTPase.
IPR029295. SnAC.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF07533. BRK. 1 hit.
PF00439. Bromodomain. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07529. HSA. 1 hit.
PF08880. QLQ. 1 hit.
PF14619. SnAC. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00592. BRK. 1 hit.
SM00297. BROMO. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00573. HSA. 1 hit.
SM00951. QLQ. 1 hit.
SM01314. SnAC. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51204. HSA. 1 hit.
PS51666. QLQ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRM_DROME
AccessioniPrimary (citable) accession number: P25439
Secondary accession number(s): A4V1Z7
, A4V1Z8, Q9VUW5, Q9VUW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 18, 2001
Last modified: November 30, 2016
This is version 177 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

'Brahma' means 'fate' in India.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.