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Protein

S-(hydroxymethyl)glutathione dehydrogenase

Gene

frmA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has high formaldehyde dehydrogenase activity in the presence of glutathione and catalyzes the oxidation of normal alcohols in a reaction that is not GSH-dependent. In addition, hemithiolacetals other than those formed from GSH, including omega-thiol fatty acids, also are substrates.1 Publication

Catalytic activityi

S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.
An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Zinc 1; catalyticBy similarity1
Metal bindingi62Zinc 1; catalyticBy similarity1
Metal bindingi92Zinc 2By similarity1
Metal bindingi95Zinc 2By similarity1
Metal bindingi98Zinc 2By similarity1
Metal bindingi106Zinc 2By similarity1
Metal bindingi169Zinc 1; catalyticBy similarity1

GO - Molecular functioni

  • alcohol dehydrogenase activity, zinc-dependent Source: EcoliWiki
  • S-(hydroxymethyl)glutathione dehydrogenase activity Source: EcoliWiki
  • zinc ion binding Source: EcoliWiki

GO - Biological processi

  • ethanol oxidation Source: InterPro
  • formaldehyde catabolic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:ADHC-MONOMER.
ECOL316407:JW0347-MONOMER.
MetaCyc:ADHC-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.284)
Alternative name(s):
Alcohol dehydrogenase class-3 (EC:1.1.1.1)
Alcohol dehydrogenase class-III
Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-)
Short name:
FALDH
Short name:
FDH
Short name:
GSH-FDH
Gene namesi
Name:frmA
Synonyms:adhC
Ordered Locus Names:b0356, JW0347
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG50010. frmA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001607751 – 369S-(hydroxymethyl)glutathione dehydrogenaseAdd BLAST369

Proteomic databases

EPDiP25437.
PaxDbiP25437.
PRIDEiP25437.

Expressioni

Inductioni

Induced by formaldehyde and repressed by FrmR.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-556478,EBI-556478

Protein-protein interaction databases

BioGridi4261803. 12 interactors.
DIPiDIP-2901N.
IntActiP25437. 7 interactors.
MINTiMINT-1286770.
STRINGi511145.b0356.

Structurei

3D structure databases

ProteinModelPortaliP25437.
SMRiP25437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108I9Z. Bacteria.
COG1062. LUCA.
HOGENOMiHOG000294674.
InParanoidiP25437.
KOiK00121.
OMAiMITHVLK.
PhylomeDBiP25437.

Family and domain databases

CDDicd08300. alcohol_DH_class_III. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR014183. ADH_3.
IPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 2 hits.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02818. adh_III_F_hyde. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25437-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSRAAVAFA PGKPLEIVEI DVAPPKKGEV LIKVTHTGVC HTDAFTLSGD
60 70 80 90 100
DPEGVFPVVL GHEGAGVVVE VGEGVTSVKP GDHVIPLYTA ECGECEFCRS
110 120 130 140 150
GKTNLCVAVR ETQGKGLMPD GTTRFSYNGQ PLYHYMGCST FSEYTVVAEV
160 170 180 190 200
SLAKINPEAN HEHVCLLGCG VTTGIGAVHN TAKVQPGDSV AVFGLGAIGL
210 220 230 240 250
AVVQGARQAK AGRIIAIDTN PKKFDLARRF GATDCINPND YDKPIKDVLL
260 270 280 290 300
DINKWGIDHT FECIGNVNVM RAALESAHRG WGQSVIIGVA VAGQEISTRP
310 320 330 340 350
FQLVTGRVWK GSAFGGVKGR SQLPGMVEDA MKGDIDLEPF VTHTMSLDEI
360
NDAFDLMHEG KSIRTVIRY
Length:369
Mass (Da):39,359
Last modified:November 1, 1997 - v3
Checksum:i35B59078F8173521
GO

Sequence cautioni

The sequence AAB18081 differs from that shown. Reason: Frameshift at position 26.Curated
The sequence BAA12834 differs from that shown. Reason: Frameshift at position 26.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41H → E AA sequence (PubMed:1731906).Curated1
Sequence conflicti46T → G AA sequence (PubMed:1731906).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85613 Genomic DNA. Translation: BAA12834.1. Frameshift.
D38504 Genomic DNA. Translation: BAA22412.1.
U73857 Genomic DNA. Translation: AAB18081.1. Frameshift.
U00096 Genomic DNA. Translation: AAC73459.1.
AP009048 Genomic DNA. Translation: BAE76138.1.
PIRiD64763.
RefSeqiNP_414890.1. NC_000913.3.
WP_000842106.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73459; AAC73459; b0356.
BAE76138; BAE76138; BAE76138.
GeneIDi944988.
KEGGiecj:JW0347.
eco:b0356.
PATRICi32115851. VBIEscCol129921_0367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85613 Genomic DNA. Translation: BAA12834.1. Frameshift.
D38504 Genomic DNA. Translation: BAA22412.1.
U73857 Genomic DNA. Translation: AAB18081.1. Frameshift.
U00096 Genomic DNA. Translation: AAC73459.1.
AP009048 Genomic DNA. Translation: BAE76138.1.
PIRiD64763.
RefSeqiNP_414890.1. NC_000913.3.
WP_000842106.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP25437.
SMRiP25437.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261803. 12 interactors.
DIPiDIP-2901N.
IntActiP25437. 7 interactors.
MINTiMINT-1286770.
STRINGi511145.b0356.

Proteomic databases

EPDiP25437.
PaxDbiP25437.
PRIDEiP25437.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73459; AAC73459; b0356.
BAE76138; BAE76138; BAE76138.
GeneIDi944988.
KEGGiecj:JW0347.
eco:b0356.
PATRICi32115851. VBIEscCol129921_0367.

Organism-specific databases

EchoBASEiEB4303.
EcoGeneiEG50010. frmA.

Phylogenomic databases

eggNOGiENOG4108I9Z. Bacteria.
COG1062. LUCA.
HOGENOMiHOG000294674.
InParanoidiP25437.
KOiK00121.
OMAiMITHVLK.
PhylomeDBiP25437.

Enzyme and pathway databases

BioCyciEcoCyc:ADHC-MONOMER.
ECOL316407:JW0347-MONOMER.
MetaCyc:ADHC-MONOMER.

Miscellaneous databases

PROiP25437.

Family and domain databases

CDDicd08300. alcohol_DH_class_III. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR014183. ADH_3.
IPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 2 hits.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02818. adh_III_F_hyde. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFRMA_ECOLI
AccessioniPrimary (citable) accession number: P25437
Secondary accession number(s): P75696, Q2MC68, Q47533
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 148 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.