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Protein

Beta-nerve growth factor

Gene

Ngf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI.By similarity

GO - Molecular functioni

GO - Biological processi

  • activation of MAPKK activity Source: Reactome
  • activation of phospholipase C activity Source: Reactome
  • adult locomotory behavior Source: RGD
  • aging Source: RGD
  • apoptotic signaling pathway Source: Reactome
  • inflammatory response Source: RGD
  • memory Source: RGD
  • negative regulation of apoptotic process Source: Reactome
  • negative regulation of cell cycle Source: Reactome
  • negative regulation of endopeptidase activity Source: GOC
  • negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
  • negative regulation of neuron apoptotic process Source: UniProtKB
  • negative regulation of type B pancreatic cell apoptotic process Source: RGD
  • nerve growth factor processing Source: Reactome
  • neuron projection development Source: RGD
  • neurotrophin TRK receptor signaling pathway Source: Reactome
  • positive regulation of apoptotic process Source: Reactome
  • positive regulation of cell growth Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of neuron differentiation Source: RGD
  • positive regulation of neurotrophin TRK receptor signaling pathway Source: MGI
  • positive regulation of protein autophosphorylation Source: MGI
  • positive regulation of protein binding Source: UniProtKB
  • positive regulation of protein phosphorylation Source: UniProtKB
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • positive regulation of stem cell proliferation Source: RGD
  • Ras protein signal transduction Source: Reactome
  • response to drug Source: RGD
  • response to electrical stimulus Source: RGD
  • response to glucocorticoid Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to mechanical stimulus Source: RGD
  • response to nicotine Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organic substance Source: RGD
  • response to ozone Source: RGD
  • response to peptide hormone Source: RGD
  • response to radiation Source: RGD
  • sensory perception of pain Source: RGD
  • small GTPase mediated signal transduction Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor

Enzyme and pathway databases

ReactomeiR-RNO-167021. PLC-gamma1 signalling.
R-RNO-167044. Signalling to RAS.
R-RNO-167060. NGF processing.
R-RNO-170968. Frs2-mediated activation.
R-RNO-170984. ARMS-mediated activation.
R-RNO-177504. Retrograde neurotrophin signalling.
R-RNO-187042. TRKA activation by NGF.
R-RNO-187706. Signalling to p38 via RIT and RIN.
R-RNO-193648. NRAGE signals death through JNK.
R-RNO-193670. p75NTR negatively regulates cell cycle via SC1.
R-RNO-193681. Ceramide signalling.
R-RNO-198203. PI3K/AKT activation.
R-RNO-198745. Signalling to STAT3.
R-RNO-205017. NFG and proNGF binds to p75NTR.
R-RNO-205025. NADE modulates death signalling.
R-RNO-205043. NRIF signals cell death from the nucleus.
R-RNO-209543. p75NTR recruits signalling complexes.
R-RNO-209560. NF-kB is activated and signals survival.
R-RNO-209563. Axonal growth stimulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-nerve growth factor
Short name:
Beta-NGF
Gene namesi
Name:Ngf
Synonyms:Ngfb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1598328. Ngf.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum lumen Source: Reactome
  • extracellular region Source: Reactome
  • extracellular space Source: RGD
  • Golgi lumen Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi221 – 2211R → W: The mutant protein is unable to activate the NTRK1 receptor. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Propeptidei19 – 121103Sequence analysisPRO_0000019609Add
BLAST
Chaini122 – 241120Beta-nerve growth factorPRO_0000019610Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691N-linked (GlcNAc...)Sequence analysis
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence analysis
Disulfide bondi136 ↔ 201By similarity
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence analysis
Disulfide bondi179 ↔ 229By similarity
Disulfide bondi189 ↔ 231By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP25427.

Expressioni

Gene expression databases

ExpressionAtlasiP25427. baseline and differential.
GenevisibleiP25427. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with ADAM10 in a divalent cation-dependent manner.By similarity

Protein-protein interaction databases

MINTiMINT-223347.
STRINGi10116.ENSRNOP00000022200.

Structurei

3D structure databases

ProteinModelPortaliP25427.
SMRiP25427. Positions 129-239.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIPQ. Eukaryota.
ENOG4111F87. LUCA.
GeneTreeiENSGT00390000007725.
HOGENOMiHOG000231516.
HOVERGENiHBG006494.
InParanoidiP25427.
KOiK02582.
OMAiNNVFKQY.
OrthoDBiEOG7RBZ8Z.
PhylomeDBiP25427.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR020408. Nerve_growth_factor-like.
IPR002072. Nerve_growth_factor-rel.
IPR020425. Nerve_growth_factor_bsu.
IPR020437. Nerve_growth_factor_bsu_mml.
IPR019846. Nerve_growth_factor_CS.
[Graphical view]
PANTHERiPTHR11589. PTHR11589. 1 hit.
PfamiPF00243. NGF. 1 hit.
[Graphical view]
PIRSFiPIRSF001789. NGF. 1 hit.
PRINTSiPR01925. MAMLNGFBETA.
PR00268. NGF.
PR01913. NGFBETA.
ProDomiPD002052. Nerve_growth_factor-rel. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00140. NGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00248. NGF_1. 1 hit.
PS50270. NGF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25427-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMLFYTLIT AFLIGVQAEP YTDSNVPEGD SVPEAHWTKL QHSLDTALRR
60 70 80 90 100
ARSAPAEPIA ARVTGQTRNI TVDPKLFKKR RLRSPRVLFS TQPPPTSSDT
110 120 130 140 150
LDLDFQAHGT ISFNRTHRSK RSSTHPVFHM GEFSVCDSVS VWVGDKTTAT
160 170 180 190 200
DIKGKEVTVL GEVNINNSVF KQYFFETKCR APNPVESGCR GIDSKHWNSY
210 220 230 240
CTTTHTFVKA LTTDDKQAAW RFIRIDTACV CVLSRKAARR G
Length:241
Mass (Da):27,010
Last modified:February 1, 1996 - v2
Checksum:i665F42371563213D
GO

Sequence cautioni

The sequence AAA41697.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36589 Genomic DNA. Translation: AAA41697.1. Different initiation.
PIRiI56570.
RefSeqiNP_001263984.1. NM_001277055.1.
XP_006233114.1. XM_006233052.2.
XP_006233115.1. XM_006233053.2.
UniGeneiRn.22168.

Genome annotation databases

EnsembliENSRNOT00000022200; ENSRNOP00000022200; ENSRNOG00000016571.
GeneIDi310738.
KEGGirno:310738.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36589 Genomic DNA. Translation: AAA41697.1. Different initiation.
PIRiI56570.
RefSeqiNP_001263984.1. NM_001277055.1.
XP_006233114.1. XM_006233052.2.
XP_006233115.1. XM_006233053.2.
UniGeneiRn.22168.

3D structure databases

ProteinModelPortaliP25427.
SMRiP25427. Positions 129-239.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-223347.
STRINGi10116.ENSRNOP00000022200.

Proteomic databases

PaxDbiP25427.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022200; ENSRNOP00000022200; ENSRNOG00000016571.
GeneIDi310738.
KEGGirno:310738.

Organism-specific databases

CTDi4803.
RGDi1598328. Ngf.

Phylogenomic databases

eggNOGiENOG410IIPQ. Eukaryota.
ENOG4111F87. LUCA.
GeneTreeiENSGT00390000007725.
HOGENOMiHOG000231516.
HOVERGENiHBG006494.
InParanoidiP25427.
KOiK02582.
OMAiNNVFKQY.
OrthoDBiEOG7RBZ8Z.
PhylomeDBiP25427.

Enzyme and pathway databases

ReactomeiR-RNO-167021. PLC-gamma1 signalling.
R-RNO-167044. Signalling to RAS.
R-RNO-167060. NGF processing.
R-RNO-170968. Frs2-mediated activation.
R-RNO-170984. ARMS-mediated activation.
R-RNO-177504. Retrograde neurotrophin signalling.
R-RNO-187042. TRKA activation by NGF.
R-RNO-187706. Signalling to p38 via RIT and RIN.
R-RNO-193648. NRAGE signals death through JNK.
R-RNO-193670. p75NTR negatively regulates cell cycle via SC1.
R-RNO-193681. Ceramide signalling.
R-RNO-198203. PI3K/AKT activation.
R-RNO-198745. Signalling to STAT3.
R-RNO-205017. NFG and proNGF binds to p75NTR.
R-RNO-205025. NADE modulates death signalling.
R-RNO-205043. NRIF signals cell death from the nucleus.
R-RNO-209543. p75NTR recruits signalling complexes.
R-RNO-209560. NF-kB is activated and signals survival.
R-RNO-209563. Axonal growth stimulation.

Miscellaneous databases

PROiP25427.

Gene expression databases

ExpressionAtlasiP25427. baseline and differential.
GenevisibleiP25427. RN.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR020408. Nerve_growth_factor-like.
IPR002072. Nerve_growth_factor-rel.
IPR020425. Nerve_growth_factor_bsu.
IPR020437. Nerve_growth_factor_bsu_mml.
IPR019846. Nerve_growth_factor_CS.
[Graphical view]
PANTHERiPTHR11589. PTHR11589. 1 hit.
PfamiPF00243. NGF. 1 hit.
[Graphical view]
PIRSFiPIRSF001789. NGF. 1 hit.
PRINTSiPR01925. MAMLNGFBETA.
PR00268. NGF.
PR01913. NGFBETA.
ProDomiPD002052. Nerve_growth_factor-rel. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00140. NGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00248. NGF_1. 1 hit.
PS50270. NGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rat beta-nerve growth factor sequence and site of synthesis in the adult hippocampus."
    Whittemore S.R., Friedman P.L., Larhammar D.G., Persson H., Gonzalez-Carvajal M., Holets V.R.
    J. Neurosci. Res. 20:403-410(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary."
    Hallboeoek F., Ibanez C.F., Persson H.
    Neuron 6:845-858(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 178-219.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. "A novel NGF mutation clarifies the molecular mechanism and extends the phenotypic spectrum of the HSAN5 neuropathy."
    Carvalho O.P., Thornton G.K., Hertecant J., Houlden H., Nicholas A.K., Cox J.J., Rielly M., Al-Gazali L., Woods C.G.
    J. Med. Genet. 48:131-135(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ARG-221.

Entry informationi

Entry nameiNGF_RAT
AccessioniPrimary (citable) accession number: P25427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.