P25427 (NGF_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-nerve growth factor
Short name=Beta-NGF

Gene names

Name:	Ngf
Synonyms:	Ngfb

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival By similarity.
Subunit structure	Homodimer.
Subcellular location	Secreted.
Sequence similarities	Belongs to the NGF-beta family.
Sequence caution	The sequence AAA41697.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	adult locomotory behavior Inferred from mutant phenotype. Source: RGD inflammatory response Inferred from expression pattern. Source: RGD memory Inferred from mutant phenotype. Source: RGD neuron projection development Inferred from direct assay. Source: RGD positive regulation of nerve growth factor receptor signaling pathway Inferred from direct assay. Source: MGI positive regulation of neuron differentiation Inferred from direct assay. Source: RGD positive regulation of protein autophosphorylation Inferred from direct assay. Source: MGI positive regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay. Source: MGI positive regulation of stem cell proliferation Inferred from direct assay. Source: RGD response to drug Inferred from expression pattern. Source: RGD response to electrical stimulus Inferred from expression pattern. Source: RGD response to glucocorticoid stimulus Inferred from expression pattern. Source: RGD response to lipopolysaccharide Inferred from expression pattern. Source: RGD response to mechanical stimulus Inferred from expression pattern. Source: RGD response to nicotine Inferred from expression pattern. Source: RGD response to ozone Inferred from expression pattern. Source: RGD response to peptide hormone stimulus Inferred from expression pattern. Source: RGD response to radiation Inferred from expression pattern. Source: RGD sensory perception of pain Inferred from mutant phenotype. Source: RGD
Cellular component	Golgi lumen Traceable author statement. Source: Reactome endoplasmic reticulum lumen Traceable author statement. Source: Reactome extracellular space Inferred from direct assay. Source: RGD
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 121	103	Potential		PRO_0000019609
	Chain	122 – 241	120	Beta-nerve growth factor		PRO_0000019610
Amino acid modifications
	Modified residue	84	1	Phosphoserine By similarity
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
	Disulfide bond	136 ↔ 201		By similarity
	Disulfide bond	179 ↔ 229		By similarity
	Disulfide bond	189 ↔ 231		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25427 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 665F42371563213D	FASTA	241	27,010
	10 20 30 40 50 60 MSMLFYTLIT AFLIGVQAEP YTDSNVPEGD SVPEAHWTKL QHSLDTALRR ARSAPAEPIA 70 80 90 100 110 120 ARVTGQTRNI TVDPKLFKKR RLRSPRVLFS TQPPPTSSDT LDLDFQAHGT ISFNRTHRSK 130 140 150 160 170 180 RSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL GEVNINNSVF KQYFFETKCR 190 200 210 220 230 240 APNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDDKQAAW RFIRIDTACV CVLSRKAARR G

References

[1]	"Rat beta-nerve growth factor sequence and site of synthesis in the adult hippocampus." Whittemore S.R., Friedman P.L., Larhammar D.G., Persson H., Gonzalez-Carvajal M., Holets V.R. J. Neurosci. Res. 20:403-410(1988) [PubMed: 3184206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary." Hallboeoek F., Ibanez C.F., Persson H. Neuron 6:845-858(1991) [PubMed: 2025430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 178-219. Strain: Sprague-Dawley. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M36589 Genomic DNA. Translation: AAA41697.1. Different initiation.
IPI	IPI00368881.
PIR	I56570.
UniGene	Rn.22168.
3D structure databases
ProteinModelPortal	P25427.
SMR	P25427. Positions 129-239.
ModBase	Search...
Protein-protein interaction databases
STRING	P25427.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
NMPDR	fig|10116.3.peg.16731.
Organism-specific databases
RGD	1598328. Ngf.
Phylogenomic databases
eggNOG	maNOG12976.
GeneTree	ENSGT00390000007725.
HOVERGEN	HBG006494.
InParanoid	P25427.
OrthoDB	EOG4BRWMZ.
Enzyme and pathway databases
Reactome	REACT_111984. Signal Transduction.
Gene expression databases
ArrayExpress	P25427.
Genevestigator	P25427.
GermOnline	ENSRNOG00000016571. Rattus norvegicus.
Family and domain databases
InterPro	IPR020408. Nerve_growth_factor-like. IPR002072. Nerve_growth_factor-rel. IPR020425. Nerve_growth_factor_bsu. IPR020437. Nerve_growth_factor_bsu_mml. IPR019846. Nerve_growth_factor_CS. [Graphical view]
PANTHER	PTHR11589. NGF. 1 hit.
Pfam	PF00243. NGF. 1 hit. [Graphical view]
PIRSF	PIRSF001789. NGF. 1 hit.
PRINTS	PR01925. MAMLNGFBETA. PR00268. NGF. PR01913. NGFBETA.
ProDom	PD002052. Nerve_growth_factor-rel. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00140. NGF. 1 hit. [Graphical view]
PROSITE	PS00248. NGF_1. 1 hit. PS50270. NGF_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NGF_RAT

Accession

Primary (citable) accession number: P25427

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program