Reviewed, UniProtKB/Swiss-Prot P25427 (NGF_RAT)

Names and origin

Protein names

Recommended name:
    Beta-nerve growth factor
      Short name=Beta-NGF

Gene names

Name:	Ngf
Synonyms:	Ngfb

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
Subunit structure	Homodimer.
Subcellular location	Secreted.
Sequence similarities	Belongs to the NGF-beta family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	adult locomotory behavior Inferred from mutant phenotype. Source: RGD inflammatory response Inferred from expression pattern. Source: RGD memory Inferred from mutant phenotype. Source: RGD neuron projection development Inferred from direct assay. Source: RGD positive regulation of cell growth Inferred from direct assay. Source: RGD positive regulation of cell proliferation Inferred from direct assay. Source: RGD response to drug Inferred from expression pattern. Source: RGD response to electrical stimulus Inferred from expression pattern. Source: RGD response to glucocorticoid stimulus Inferred from expression pattern. Source: RGD response to lipopolysaccharide Inferred from expression pattern. Source: RGD response to mechanical stimulus Inferred from expression pattern. Source: RGD response to organic cyclic substance Inferred from expression pattern. Source: RGD response to peptide hormone stimulus Inferred from expression pattern. Source: RGD response to radiation Inferred from expression pattern. Source: RGD
Cellular component	Golgi lumen Inferred from Experiment. Source: Reactome endoplasmic reticulum lumen Inferred from Experiment. Source: Reactome extracellular space Inferred from direct assay. Source: RGD
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 121	103	Potential		PRO_0000019609
	Chain	122 – 241	120	Beta-nerve growth factor		PRO_0000019610
Amino acid modifications
	Modified residue	84	1	Phosphoserine By similarity
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
	Disulfide bond	136 ↔ 201		By similarity
	Disulfide bond	179 ↔ 229		By similarity
	Disulfide bond	189 ↔ 231		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25427-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 665F42371563213D	FASTA	241	27,010
	10 20 30 40 50 60 MSMLFYTLIT AFLIGVQAEP YTDSNVPEGD SVPEAHWTKL QHSLDTALRR ARSAPAEPIA 70 80 90 100 110 120 ARVTGQTRNI TVDPKLFKKR RLRSPRVLFS TQPPPTSSDT LDLDFQAHGT ISFNRTHRSK 130 140 150 160 170 180 RSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL GEVNINNSVF KQYFFETKCR 190 200 210 220 230 240 APNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDDKQAAW RFIRIDTACV CVLSRKAARR G

References

[1]	"Rat beta-nerve growth factor sequence and site of synthesis in the adult hippocampus." Whittemore S.R., Friedman P.L., Larhammar D.G., Persson H., Gonzalez-Carvajal M., Holets V.R. J. Neurosci. Res. 20:403-410(1988) [PubMed: 3184206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary." Hallboeoek F., Ibanez C.F., Persson H. Neuron 6:845-858(1991) [PubMed: 2025430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 178-219. Strain: Sprague-Dawley. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M36589 Genomic DNA. Translation: AAA41697.1. Different initiation.
IPI	IPI00368881.
PIR	I56570.
UniGene	Rn.22168
3D structure databases
HSSP	HSSP built from PDB template 1BET based on UniProtKB P01139.
SMR	P25427. Positions 129-239.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000016571. Rattus norvegicus. [Contig view]
NMPDR	fig|10116.3.peg.16731.
Organism-specific databases
RGD	1598328. Ngf.
Phylogenomic databases
HOVERGEN	P25427.
Gene expression databases
ArrayExpress	P25427.
GermOnline	ENSRNOG00000016571. Rattus norvegicus.
Family and domain databases
InterPro	IPR002072. Nerve_growth_factor-rel. IPR019846. Nerve_growth_factor_CS. [Graphical view]
PANTHER	PTHR11589. NGF. 1 hit.
Pfam	PF00243. NGF. 1 hit. [Graphical view]
PIRSF	PIRSF001789. NGF. 1 hit.
PRINTS	PR00268. NGF.
ProDom	PD002052. NGF. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00140. NGF. 1 hit. [Graphical view]
PROSITE	PS00248. NGF_1. 1 hit. PS50270. NGF_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NGF_RAT

Accession

Primary (citable) accession number: P25427

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)