Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

POU domain, class 2, transcription factor 1

Gene

Pou2f1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi382 – 441HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: MGI
  • sequence-specific DNA binding Source: MGI
  • transcription factor activity, sequence-specific DNA binding Source: InterPro

GO - Biological processi

  • lens induction in camera-type eye Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • olfactory placode formation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-6807505. RNA polymerase II transcribes snRNA genes.
R-MMU-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 2, transcription factor 1
Alternative name(s):
NF-A1
Octamer-binding protein 1
Short name:
Oct-1
Octamer-binding transcription factor 1
Short name:
OTF-1
Gene namesi
Name:Pou2f1
Synonyms:Oct-1, Otf-1, Otf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:101898. Pou2f1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007081 – 770POU domain, class 2, transcription factor 1Add BLAST770

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei271PhosphothreonineBy similarity1
Modified residuei277PhosphothreonineBy similarity1
Modified residuei284PhosphoserineBy similarity1
Modified residuei388PhosphoserineBy similarity1
Modified residuei451PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by PRKDC.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25425.
PaxDbiP25425.
PeptideAtlasiP25425.
PRIDEiP25425.

PTM databases

iPTMnetiP25425.
PhosphoSitePlusiP25425.

Expressioni

Tissue specificityi

Ubiquitously expressed. However, isoforms 4 and 5 are only expressed in lymphocytes.4 Publications

Gene expression databases

BgeeiENSMUSG00000026565.
CleanExiMM_POU2F1.
ExpressionAtlasiP25425. baseline and differential.
GenevisibleiP25425. MM.

Interactioni

Subunit structurei

Interacts with NR3C1, AR, PGR and HCFC1.By similarity

Protein-protein interaction databases

BioGridi202301. 16 interactors.
STRINGi10090.ENSMUSP00000124738.

Structurei

3D structure databases

ProteinModelPortaliP25425.
SMRiP25425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini281 – 355POU-specificPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
GeneTreeiENSGT00760000118935.
HOVERGENiHBG057016.
InParanoidiP25425.
KOiK09364.
PhylomeDBiP25425.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
IPR000972. TF_octamer.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (12)i

Sequence statusi: Complete.

This entry describes 12 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P25425-1) [UniParc]FASTAAdd to basket
Also known as: OCT-1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNNPSETNKS SMESEDASTG TQTNGLDFQK QPVPVGGAIS TAQAQAFLGH
60 70 80 90 100
LHQVQLAGTS LQAAAQSLNV QSKSSEESGD SQQSSQPSSQ PPSVQSAIPQ
110 120 130 140 150
TQLMLAGGQI TGLTLTPAQQ QLLLQQAQAQ AQLLAAAVQQ HSASQQHSAA
160 170 180 190 200
GATISASAAT PMTQIPLSQP IQIAQDLQQL QQLQQQNLNL QQFVLVHPTT
210 220 230 240 250
NLQPAQFIIS QTPQGQQGLL QAQNLLTQLP QQSQANLLQP QPSITLTSQP
260 270 280 290 300
TTPTRTIAAA SVQTLPQSQS TPKRIDTPSL EEPSDLEELE QFAKTFKQRR
310 320 330 340 350
IKLGFTQGDV GLAMGKLYGN DFSQTTISRF EALNLSFKNM CKLKPLLEKW
360 370 380 390 400
LNDAENLSSD STASSPSALN SPGLGAEGLN RRRKKRTSIE TNIRVALEKS
410 420 430 440 450
FMENQKPTSE DITLIAEQLN MEKEVIRVWF CNRRQKEKRI NPPSSGGTSS
460 470 480 490 500
SPIKAIFPSP ASLVATTPSL VTSSTATTLT VNPVLPLTSA AVTNLSLTDQ
510 520 530 540 550
DLRRGCSWEV LRSLPDRVTT TAGTTDSTSN NNTATVISTA PPASSAVTSP
560 570 580 590 600
SLSPSPSASA STSEASSASE TNTTQTTSTP LPSPLGASQV MVTTPGLQTA
610 620 630 640 650
AAALQGAAQL PANASLAAMA AAAGLSPGLM APSQFAAGGA LLSLSPGTLG
660 670 680 690 700
SALSPALMSN STLATIQALA SSGSLPITSL DATGNLVFAN AGGAPNIVTA
710 720 730 740 750
PLFLNPQNLS LLTSNPVSLV SAAAASTGNS APTASLHASS TSTESIQSSL
760 770
FTVASASGPA STTTAASKAQ
Length:770
Mass (Da):79,547
Last modified:April 23, 2003 - v3
Checksum:iB45CDBE632853FE9
GO
Isoform 2 (identifier: P25425-2) [UniParc]FASTAAdd to basket
Also known as: OCT-1B

The sequence of this isoform differs from the canonical sequence as follows:
     499-522: Missing.

Show »
Length:746
Mass (Da):76,790
Checksum:iA19E2D4CB6C147A2
GO
Isoform 3 (identifier: P25425-3) [UniParc]FASTAAdd to basket
Also known as: OCT-1C

The sequence of this isoform differs from the canonical sequence as follows:
     499-522: Missing.
     717-725: VSLVSAAAA → DCFMDWRTF
     726-770: Missing.

Show »
Length:701
Mass (Da):72,983
Checksum:i85CEC91D2241D0D9
GO
Isoform 4 (identifier: P25425-4) [UniParc]FASTAAdd to basket
Also known as: OCT-1R

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MLDCSDCVLDSRM
     54-71: Missing.
     499-522: Missing.
     639-770: Missing.

Show »
Length:608
Mass (Da):63,780
Checksum:iED52028C67A99D5C
GO
Isoform 5 (identifier: P25425-5) [UniParc]FASTAAdd to basket
Also known as: OCT-1L

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MLDCSDCVLDSRM
     499-522: Missing.

Show »
Length:758
Mass (Da):78,128
Checksum:iEF08D83A224B2418
GO
Isoform 6 (identifier: P25425-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     668-691: Missing.

Show »
Length:746
Mass (Da):77,274
Checksum:iAEBEE16A471595D4
GO
Isoform 7 (identifier: P25425-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     639-641: GAL → SCF
     642-770: Missing.

Show »
Length:641
Mass (Da):67,317
Checksum:iBD83AEBFA5C51643
GO
Isoform 8 (identifier: P25425-8) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     668-691: Missing.
     717-725: VSLVSAAAA → DCFMDWRTF
     726-770: Missing.

Show »
Length:701
Mass (Da):73,467
Checksum:iF1092FC5322C5061
GO
Isoform 9 (identifier: P25425-9) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     639-647: GALLSLSPG → IASWIGELS
     648-770: Missing.

Show »
Length:647
Mass (Da):67,937
Checksum:iD59952DB1E8D61F0
GO
Isoform 10 (identifier: P25425-10) [UniParc]FASTAAdd to basket
Also known as: OCT-1Z

The sequence of this isoform differs from the canonical sequence as follows:
     499-522: DQDLRRGCSWEVLRSLPDRVTTTA → GKQQPAYRLVSTVPVRFLWRTARS
     523-770: Missing.

Show »
Length:522
Mass (Da):56,193
Checksum:i6ABAAA323C1F873B
GO
Isoform 11 (identifier: P25425-11) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MLDCSDCVLDSRM
     639-770: Missing.

Show »
Length:650
Mass (Da):68,318
Checksum:i649DDF3D7105D736
GO
Isoform 12 (identifier: P25425-12) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MADGGAASQDESSAAAAAAADSR

Note: No experimental confirmation available.
Show »
Length:792
Mass (Da):81,479
Checksum:i96B1978640108D06
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43Q → L in CAA39679 (PubMed:1561098).Curated1
Sequence conflicti70V → I in CAC34943 (PubMed:11683265).Curated1
Sequence conflicti129A → P (PubMed:7711063).Curated1
Sequence conflicti168S → C in CAD35743 (Ref. 6) Curated1
Sequence conflicti188L → H in CAC34943 (PubMed:11683265).Curated1
Sequence conflicti234Q → R in CAC34943 (PubMed:11683265).Curated1
Sequence conflicti333L → W (PubMed:8376772).Curated1
Sequence conflicti360D → G in CAC34943 (PubMed:11683265).Curated1
Sequence conflicti368A → D (PubMed:1970171).Curated1
Sequence conflicti441N → D in CAC34943 (PubMed:11683265).Curated1
Sequence conflicti474S → G in CAC34943 (PubMed:11683265).Curated1
Sequence conflicti596G → D in CAC34943 (PubMed:11683265).Curated1
Sequence conflicti608A → G in CAA48422 (PubMed:8441632).Curated1
Sequence conflicti608A → G in CAA48423 (PubMed:8441632).Curated1
Sequence conflicti608A → G in CAA48424 (PubMed:8441632).Curated1
Sequence conflicti609Q → R in CAC34943 (PubMed:11683265).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0134031M → MADGGAASQDESSAAAAAAA DSR in isoform 12. 1 Publication1
Alternative sequenceiVSP_0072711M → MLDCSDCVLDSRM in isoform 4, isoform 5 and isoform 11. 3 Publications1
Alternative sequenceiVSP_00727254 – 71Missing in isoform 4. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_013404499 – 522DQDLR…VTTTA → GKQQPAYRLVSTVPVRFLWR TARS in isoform 10. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_002321499 – 522Missing in isoform 2, isoform 3, isoform 4 and isoform 5. 5 PublicationsAdd BLAST24
Alternative sequenceiVSP_013405523 – 770Missing in isoform 10. 1 PublicationAdd BLAST248
Alternative sequenceiVSP_007273639 – 770Missing in isoform 4 and isoform 11. 2 PublicationsAdd BLAST132
Alternative sequenceiVSP_007274639 – 647GALLSLSPG → IASWIGELS in isoform 9. 1 Publication9
Alternative sequenceiVSP_007276639 – 641GAL → SCF in isoform 7. 1 Publication3
Alternative sequenceiVSP_007277642 – 770Missing in isoform 7. 1 PublicationAdd BLAST129
Alternative sequenceiVSP_007275648 – 770Missing in isoform 9. 1 PublicationAdd BLAST123
Alternative sequenceiVSP_007278668 – 691Missing in isoform 6 and isoform 8. 2 PublicationsAdd BLAST24
Alternative sequenceiVSP_002322717 – 725VSLVSAAAA → DCFMDWRTF in isoform 3 and isoform 8. 2 Publications9
Alternative sequenceiVSP_002323726 – 770Missing in isoform 3 and isoform 8. 2 PublicationsAdd BLAST45

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68362 mRNA. Translation: CAA48422.1.
X68363 mRNA. Translation: CAA48423.1.
X68364 mRNA. Translation: CAA48424.1.
X56230 mRNA. Translation: CAA39679.1.
AJ296212 mRNA. Translation: CAC34943.1.
AF508939 mRNA. Translation: AAM34281.1.
AY177625 mRNA. Translation: AAO45298.1.
AJ489474 mRNA. Translation: CAD35743.1.
AK028237 mRNA. Translation: BAC25832.1.
S65461 mRNA. Translation: AAB28234.1.
X70324 mRNA. Translation: CAA49791.1.
X70325 mRNA. Translation: CAA49792.1.
X51958 mRNA. Translation: CAA36217.1.
AF095458 mRNA. Translation: AAD25325.1.
AF095459 mRNA. Translation: AAD25326.1.
AF095460 mRNA. Translation: AAD25327.1.
CCDSiCCDS15444.1. [P25425-5]
PIRiI56187.
S30293.
RefSeqiNP_035267.2. NM_011137.3.
NP_945151.2. NM_198933.3. [P25425-5]
XP_006496767.1. XM_006496704.2. [P25425-1]
XP_011237069.1. XM_011238767.2. [P25425-1]
XP_011237071.1. XM_011238769.2. [P25425-1]
XP_017174849.1. XM_017319360.1. [P25425-2]
UniGeneiMm.245261.

Genome annotation databases

EnsembliENSMUST00000069609; ENSMUSP00000064000; ENSMUSG00000026565. [P25425-5]
ENSMUST00000111429; ENSMUSP00000107057; ENSMUSG00000026565. [P25425-2]
ENSMUST00000184643; ENSMUSP00000138962; ENSMUSG00000026565. [P25425-3]
GeneIDi18986.
KEGGimmu:18986.
UCSCiuc007djv.1. mouse. [P25425-6]
uc007djx.2. mouse. [P25425-5]
uc007dkb.2. mouse. [P25425-4]
uc007dkc.2. mouse. [P25425-11]
uc007dke.1. mouse. [P25425-10]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68362 mRNA. Translation: CAA48422.1.
X68363 mRNA. Translation: CAA48423.1.
X68364 mRNA. Translation: CAA48424.1.
X56230 mRNA. Translation: CAA39679.1.
AJ296212 mRNA. Translation: CAC34943.1.
AF508939 mRNA. Translation: AAM34281.1.
AY177625 mRNA. Translation: AAO45298.1.
AJ489474 mRNA. Translation: CAD35743.1.
AK028237 mRNA. Translation: BAC25832.1.
S65461 mRNA. Translation: AAB28234.1.
X70324 mRNA. Translation: CAA49791.1.
X70325 mRNA. Translation: CAA49792.1.
X51958 mRNA. Translation: CAA36217.1.
AF095458 mRNA. Translation: AAD25325.1.
AF095459 mRNA. Translation: AAD25326.1.
AF095460 mRNA. Translation: AAD25327.1.
CCDSiCCDS15444.1. [P25425-5]
PIRiI56187.
S30293.
RefSeqiNP_035267.2. NM_011137.3.
NP_945151.2. NM_198933.3. [P25425-5]
XP_006496767.1. XM_006496704.2. [P25425-1]
XP_011237069.1. XM_011238767.2. [P25425-1]
XP_011237071.1. XM_011238769.2. [P25425-1]
XP_017174849.1. XM_017319360.1. [P25425-2]
UniGeneiMm.245261.

3D structure databases

ProteinModelPortaliP25425.
SMRiP25425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202301. 16 interactors.
STRINGi10090.ENSMUSP00000124738.

PTM databases

iPTMnetiP25425.
PhosphoSitePlusiP25425.

Proteomic databases

MaxQBiP25425.
PaxDbiP25425.
PeptideAtlasiP25425.
PRIDEiP25425.

Protocols and materials databases

DNASUi18986.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069609; ENSMUSP00000064000; ENSMUSG00000026565. [P25425-5]
ENSMUST00000111429; ENSMUSP00000107057; ENSMUSG00000026565. [P25425-2]
ENSMUST00000184643; ENSMUSP00000138962; ENSMUSG00000026565. [P25425-3]
GeneIDi18986.
KEGGimmu:18986.
UCSCiuc007djv.1. mouse. [P25425-6]
uc007djx.2. mouse. [P25425-5]
uc007dkb.2. mouse. [P25425-4]
uc007dkc.2. mouse. [P25425-11]
uc007dke.1. mouse. [P25425-10]

Organism-specific databases

CTDi5451.
MGIiMGI:101898. Pou2f1.

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
GeneTreeiENSGT00760000118935.
HOVERGENiHBG057016.
InParanoidiP25425.
KOiK09364.
PhylomeDBiP25425.
TreeFamiTF316413.

Enzyme and pathway databases

ReactomeiR-MMU-6807505. RNA polymerase II transcribes snRNA genes.
R-MMU-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

ChiTaRSiPou2f1. mouse.
PROiP25425.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026565.
CleanExiMM_POU2F1.
ExpressionAtlasiP25425. baseline and differential.
GenevisibleiP25425. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
IPR000972. TF_octamer.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPO2F1_MOUSE
AccessioniPrimary (citable) accession number: P25425
Secondary accession number(s): Q61994
, Q63891, Q6Y681, Q7TSD0, Q8BT04, Q8K570, Q99JH0, Q9WTZ4, Q9WTZ5, Q9WTZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: April 23, 2003
Last modified: November 2, 2016
This is version 168 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.