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P25409

- ALAT1_RAT

UniProt

P25409 - ALAT1_RAT

Protein

Alanine aminotransferase 1

Gene

Gpt

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles By similarity.By similarity

    Catalytic activityi

    L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    GO - Molecular functioni

    1. L-alanine:2-oxoglutarate aminotransferase activity Source: RGD
    2. pyridoxal phosphate binding Source: RGD

    GO - Biological processi

    1. biosynthetic process Source: InterPro
    2. L-alanine catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_214662. Amino acid synthesis and interconversion (transamination).
    SABIO-RKP25409.
    UniPathwayiUPA00528; UER00586.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine aminotransferase 1 (EC:2.6.1.2)
    Short name:
    ALT1
    Alternative name(s):
    Glutamate pyruvate transaminase 1
    Short name:
    GPT 1
    Glutamic--alanine transaminase 1
    Glutamic--pyruvic transaminase 1
    Gene namesi
    Name:Gpt
    Synonyms:Aat1, Gpt1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi621720. Gpt.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 496495Alanine aminotransferase 1PRO_0000123936Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei314 – 3141N6-(pyridoxal phosphate)lysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP25409.
    PRIDEiP25409.

    PTM databases

    PhosphoSiteiP25409.

    Expressioni

    Tissue specificityi

    Liver, heart, skeletal muscle, etc.

    Inductioni

    By glucocorticoids.

    Gene expression databases

    GenevestigatoriP25409.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000044411.

    Structurei

    3D structure databases

    ProteinModelPortaliP25409.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0436.
    GeneTreeiENSGT00650000093331.
    HOGENOMiHOG000215020.
    HOVERGENiHBG026148.
    InParanoidiP25409.
    KOiK00814.
    OMAiHAKFTLE.
    OrthoDBiEOG76HQ18.
    PhylomeDBiP25409.
    TreeFamiTF300839.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25409-1 [UniParc]FASTAAdd to Basket

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    MASRVNDQSQ ASRNGLKGKV LTLDTMNPCV RRVEYAVRGP IVQRALELEQ    50
    ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FFRQVLALCV YPNLLSSPDF 100
    PEDAKRRAER ILQACGGHSL GAYSISSGIQ PIREDVAQYI ERRDGGIPAD 150
    PNNIFLSTGA SDAIVTMLKL LVSGEGRART GVLIPIPQYP LYSAALAELD 200
    AVQVDYYLDE ERAWALDIAE LRRALCQARD RCCPRVLCVI NPGNPTGQVQ 250
    TRECIEAVIR FAFKEGLFLM ADEVYQDNVY AEGSQFHSFK KVLMEMGPPY 300
    STQQELASFH SVSKGYMGEC GFRGGYVEVV NMDAEVQKQM GKLMSVRLCP 350
    PVPGQALMDM VVSPPTPSEP SFKQFQAERQ EVLAELAAKA KLTEQVFNEA 400
    PGIRCNPVQG AMYSFPQVQL PLKAVQRAQE LGLAPDMFFC LCLLEETGIC 450
    VVPGSGFGQQ EGTYHFRMTI LPPMEKLRLL LEKLSHFHAK FTHEYS 496
    Length:496
    Mass (Da):55,110
    Last modified:January 23, 2007 - v2
    Checksum:i7A4714E94146ABF8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10354 mRNA. Translation: BAA01185.1.
    BC097937 mRNA. Translation: AAH97937.1.
    PIRiA39900.
    RefSeqiNP_112301.1. NM_031039.1.
    UniGeneiRn.6318.

    Genome annotation databases

    EnsembliENSRNOT00000050556; ENSRNOP00000044411; ENSRNOG00000033915.
    GeneIDi81670.
    KEGGirno:81670.
    UCSCiRGD:621720. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10354 mRNA. Translation: BAA01185.1 .
    BC097937 mRNA. Translation: AAH97937.1 .
    PIRi A39900.
    RefSeqi NP_112301.1. NM_031039.1.
    UniGenei Rn.6318.

    3D structure databases

    ProteinModelPortali P25409.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000044411.

    Chemistry

    ChEMBLi CHEMBL3260.

    PTM databases

    PhosphoSitei P25409.

    Proteomic databases

    PaxDbi P25409.
    PRIDEi P25409.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000050556 ; ENSRNOP00000044411 ; ENSRNOG00000033915 .
    GeneIDi 81670.
    KEGGi rno:81670.
    UCSCi RGD:621720. rat.

    Organism-specific databases

    CTDi 2875.
    RGDi 621720. Gpt.

    Phylogenomic databases

    eggNOGi COG0436.
    GeneTreei ENSGT00650000093331.
    HOGENOMi HOG000215020.
    HOVERGENi HBG026148.
    InParanoidi P25409.
    KOi K00814.
    OMAi HAKFTLE.
    OrthoDBi EOG76HQ18.
    PhylomeDBi P25409.
    TreeFami TF300839.

    Enzyme and pathway databases

    UniPathwayi UPA00528 ; UER00586 .
    Reactomei REACT_214662. Amino acid synthesis and interconversion (transamination).
    SABIO-RK P25409.

    Miscellaneous databases

    NextBioi 615260.
    PROi P25409.

    Gene expression databases

    Genevestigatori P25409.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Tanase S.
      Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    3. "Complete amino acid sequence of rat liver cytosolic alanine aminotransferase."
      Ishiguro M., Suzuki M., Takio K., Matsuzawa T., Titani K.
      Biochemistry 30:6048-6053(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-496, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2.
      Tissue: Liver.

    Entry informationi

    Entry nameiALAT1_RAT
    AccessioniPrimary (citable) accession number: P25409
    Secondary accession number(s): Q4V7F7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3