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P25409

- ALAT1_RAT

UniProt

P25409 - ALAT1_RAT

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Protein

Alanine aminotransferase 1

Gene

Gpt

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).By similarity

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pathwayi

GO - Molecular functioni

  1. L-alanine:2-oxoglutarate aminotransferase activity Source: RGD
  2. pyridoxal phosphate binding Source: RGD

GO - Biological processi

  1. biosynthetic process Source: InterPro
  2. L-alanine catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_214662. Amino acid synthesis and interconversion (transamination).
SABIO-RKP25409.
UniPathwayiUPA00528; UER00586.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine aminotransferase 1 (EC:2.6.1.2)
Short name:
ALT1
Alternative name(s):
Glutamate pyruvate transaminase 1
Short name:
GPT 1
Glutamic--alanine transaminase 1
Glutamic--pyruvic transaminase 1
Gene namesi
Name:Gpt
Synonyms:Aat1, Gpt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi621720. Gpt.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 496495Alanine aminotransferase 1PRO_0000123936Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei314 – 3141N6-(pyridoxal phosphate)lysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP25409.
PRIDEiP25409.

PTM databases

PhosphoSiteiP25409.

Expressioni

Tissue specificityi

Liver, heart, skeletal muscle, etc.

Inductioni

By glucocorticoids.

Gene expression databases

GenevestigatoriP25409.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000044411.

Structurei

3D structure databases

ProteinModelPortaliP25409.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0436.
GeneTreeiENSGT00650000093331.
HOGENOMiHOG000215020.
HOVERGENiHBG026148.
InParanoidiP25409.
KOiK00814.
OMAiHAKFTLE.
OrthoDBiEOG76HQ18.
PhylomeDBiP25409.
TreeFamiTF300839.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25409-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASRVNDQSQ ASRNGLKGKV LTLDTMNPCV RRVEYAVRGP IVQRALELEQ
60 70 80 90 100
ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FFRQVLALCV YPNLLSSPDF
110 120 130 140 150
PEDAKRRAER ILQACGGHSL GAYSISSGIQ PIREDVAQYI ERRDGGIPAD
160 170 180 190 200
PNNIFLSTGA SDAIVTMLKL LVSGEGRART GVLIPIPQYP LYSAALAELD
210 220 230 240 250
AVQVDYYLDE ERAWALDIAE LRRALCQARD RCCPRVLCVI NPGNPTGQVQ
260 270 280 290 300
TRECIEAVIR FAFKEGLFLM ADEVYQDNVY AEGSQFHSFK KVLMEMGPPY
310 320 330 340 350
STQQELASFH SVSKGYMGEC GFRGGYVEVV NMDAEVQKQM GKLMSVRLCP
360 370 380 390 400
PVPGQALMDM VVSPPTPSEP SFKQFQAERQ EVLAELAAKA KLTEQVFNEA
410 420 430 440 450
PGIRCNPVQG AMYSFPQVQL PLKAVQRAQE LGLAPDMFFC LCLLEETGIC
460 470 480 490
VVPGSGFGQQ EGTYHFRMTI LPPMEKLRLL LEKLSHFHAK FTHEYS
Length:496
Mass (Da):55,110
Last modified:January 23, 2007 - v2
Checksum:i7A4714E94146ABF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10354 mRNA. Translation: BAA01185.1.
BC097937 mRNA. Translation: AAH97937.1.
PIRiA39900.
RefSeqiNP_112301.1. NM_031039.1.
XP_008763821.1. XM_008765599.1.
UniGeneiRn.6318.

Genome annotation databases

EnsembliENSRNOT00000050556; ENSRNOP00000044411; ENSRNOG00000033915.
GeneIDi81670.
KEGGirno:81670.
UCSCiRGD:621720. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10354 mRNA. Translation: BAA01185.1 .
BC097937 mRNA. Translation: AAH97937.1 .
PIRi A39900.
RefSeqi NP_112301.1. NM_031039.1.
XP_008763821.1. XM_008765599.1.
UniGenei Rn.6318.

3D structure databases

ProteinModelPortali P25409.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000044411.

Chemistry

ChEMBLi CHEMBL3260.

PTM databases

PhosphoSitei P25409.

Proteomic databases

PaxDbi P25409.
PRIDEi P25409.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000050556 ; ENSRNOP00000044411 ; ENSRNOG00000033915 .
GeneIDi 81670.
KEGGi rno:81670.
UCSCi RGD:621720. rat.

Organism-specific databases

CTDi 2875.
RGDi 621720. Gpt.

Phylogenomic databases

eggNOGi COG0436.
GeneTreei ENSGT00650000093331.
HOGENOMi HOG000215020.
HOVERGENi HBG026148.
InParanoidi P25409.
KOi K00814.
OMAi HAKFTLE.
OrthoDBi EOG76HQ18.
PhylomeDBi P25409.
TreeFami TF300839.

Enzyme and pathway databases

UniPathwayi UPA00528 ; UER00586 .
Reactomei REACT_214662. Amino acid synthesis and interconversion (transamination).
SABIO-RK P25409.

Miscellaneous databases

NextBioi 615260.
PROi P25409.

Gene expression databases

Genevestigatori P25409.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Tanase S.
    Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  3. "Complete amino acid sequence of rat liver cytosolic alanine aminotransferase."
    Ishiguro M., Suzuki M., Takio K., Matsuzawa T., Titani K.
    Biochemistry 30:6048-6053(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-496, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2.
    Tissue: Liver.

Entry informationi

Entry nameiALAT1_RAT
AccessioniPrimary (citable) accession number: P25409
Secondary accession number(s): Q4V7F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3