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Protein

Alanine aminotransferase 1

Gene

Gpt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).By similarity

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pathwayi: L-alanine degradation via transaminase pathway

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine aminotransferase 1 (Gpt)
This subpathway is part of the pathway L-alanine degradation via transaminase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from L-alanine, the pathway L-alanine degradation via transaminase pathway and in Amino-acid degradation.

GO - Molecular functioni

  • L-alanine:2-oxoglutarate aminotransferase activity Source: RGD
  • pyridoxal phosphate binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-RNO-70614 Amino acid synthesis and interconversion (transamination)
SABIO-RKiP25409
UniPathwayiUPA00528; UER00586

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine aminotransferase 1 (EC:2.6.1.2)
Short name:
ALT1
Alternative name(s):
Glutamate pyruvate transaminase 1
Short name:
GPT 1
Glutamic--alanine transaminase 1
Glutamic--pyruvic transaminase 1
Gene namesi
Name:Gpt
Synonyms:Aat1, Gpt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi621720 Gpt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3260

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001239362 – 496Alanine aminotransferase 1Add BLAST495

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei22PhosphothreonineBy similarity1
Modified residuei314N6-(pyridoxal phosphate)lysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP25409
PRIDEiP25409

PTM databases

iPTMnetiP25409
PhosphoSitePlusiP25409

Expressioni

Tissue specificityi

Liver, heart, skeletal muscle, etc.

Inductioni

By glucocorticoids.

Gene expression databases

BgeeiENSRNOG00000033915
GenevisibleiP25409 RN

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000044411

Structurei

3D structure databases

ProteinModelPortaliP25409
SMRiP25409
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0258 Eukaryota
COG0436 LUCA
GeneTreeiENSGT00650000093331
HOGENOMiHOG000215020
HOVERGENiHBG026148
InParanoidiP25409
KOiK00814
OMAiQMLKLMS
OrthoDBiEOG091G0PY3
PhylomeDBiP25409
TreeFamiTF300839

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25409-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASRVNDQSQ ASRNGLKGKV LTLDTMNPCV RRVEYAVRGP IVQRALELEQ
60 70 80 90 100
ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FFRQVLALCV YPNLLSSPDF
110 120 130 140 150
PEDAKRRAER ILQACGGHSL GAYSISSGIQ PIREDVAQYI ERRDGGIPAD
160 170 180 190 200
PNNIFLSTGA SDAIVTMLKL LVSGEGRART GVLIPIPQYP LYSAALAELD
210 220 230 240 250
AVQVDYYLDE ERAWALDIAE LRRALCQARD RCCPRVLCVI NPGNPTGQVQ
260 270 280 290 300
TRECIEAVIR FAFKEGLFLM ADEVYQDNVY AEGSQFHSFK KVLMEMGPPY
310 320 330 340 350
STQQELASFH SVSKGYMGEC GFRGGYVEVV NMDAEVQKQM GKLMSVRLCP
360 370 380 390 400
PVPGQALMDM VVSPPTPSEP SFKQFQAERQ EVLAELAAKA KLTEQVFNEA
410 420 430 440 450
PGIRCNPVQG AMYSFPQVQL PLKAVQRAQE LGLAPDMFFC LCLLEETGIC
460 470 480 490
VVPGSGFGQQ EGTYHFRMTI LPPMEKLRLL LEKLSHFHAK FTHEYS
Length:496
Mass (Da):55,110
Last modified:January 23, 2007 - v2
Checksum:i7A4714E94146ABF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10354 mRNA Translation: BAA01185.1
BC097937 mRNA Translation: AAH97937.1
PIRiA39900
RefSeqiNP_112301.1, NM_031039.1
UniGeneiRn.6318

Genome annotation databases

EnsembliENSRNOT00000050556; ENSRNOP00000044411; ENSRNOG00000033915
GeneIDi81670
KEGGirno:81670
UCSCiRGD:621720 rat

Similar proteinsi

Entry informationi

Entry nameiALAT1_RAT
AccessioniPrimary (citable) accession number: P25409
Secondary accession number(s): Q4V7F7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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