Reviewed, UniProtKB/Swiss-Prot P25405 (ADH1A_UROHA)

Names and origin

Protein names	Recommended name:     Alcohol dehydrogenase 1A     EC=1.1.1.1 Alternative name(s):     Alcohol dehydrogenase I-A       Short name=ADH IA
Organism	Uromastyx hardwickii (Indian spiny-tailed lizard)
Taxonomic identifier	40250 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Iguania › Acrodonta › Agamidae › Uromastycinae › Uromastyx

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	An alcohol + NAD(+) = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Multimeric (with different ratios of monomers).
Subcellular location	Cytoplasm.
Miscellaneous	In U.hardwickii there are two isozymes of alcohol dehydrogenase I.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Class-I subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 375	375	Alcohol dehydrogenase 1A
Sites
	Metal binding	46	1	Zinc 1; catalytic By similarity
	Metal binding	67	1	Zinc 1; catalytic By similarity
	Metal binding	97	1	Zinc 2 By similarity
	Metal binding	100	1	Zinc 2 By similarity
	Metal binding	103	1	Zinc 2 By similarity
	Metal binding	111	1	Zinc 2 By similarity
	Metal binding	174	1	Zinc 1; catalytic By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylglycine

Sequences

	Sequence		Length	Mass (Da)
	P25405-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: F315BB777996D906	FASTA	375	39,663
	10 20 30 40 50 60 GTAGKVIKCK AAIAWEIKKP LSIEQIEVAP PKAHEVRIKI LATGICRSDD HVISGAFKMP 70 80 90 100 110 120 LPMVLGHEAA GVVESVGEGV TCVKPGDKVI PLFVPQCGKC SSCRSTRGNL CTSNDLSAAT 130 140 150 160 170 180 GLMPDGTSRF TCKGKSLHHF ISTSSFTEYT VVHENSVVKI DAAAPLEKVC LIGCGFSTGY 190 200 210 220 230 240 GAAVETAKVE PGSTCAVFGL GGVGLSAVMG CKAAGASRII GVDINKDKFP KAKEMGATEC 250 260 270 280 290 300 VNPLDYKKPI NEVLFDLTGG EGVDYSFEVI GRTDTMTAAL ASCHMDYGTS IIVGLPPSAS 310 320 330 340 350 360 EITFSPGLIF TGRTWKGSVF GGWKSKDSVP RLVSDFMQKK FSLDPLITHT MPFDKINEGF 370 ELLRAGKSIR SVLLF

References

[1]	"Linking of isozyme and class variability patterns in the emergence of novel alcohol dehydrogenase functions. Characterization of isozymes in Uromastix hardwickii." Hjelmqvist L., Shafqat J., Siddiqi A.R., Joernvall H. Eur. J. Biochem. 236:563-570(1996) [PubMed: 8612630] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Reptilian alcohol dehydrogenase. Heterogeneity relevant to class multiplicity of the mammalian enzyme." Hjelmqvist L., Ericsson M., Shafqat J., Carlquist M., Siddiqi A.R., Hoeoeg J.-O., Joernvall H. FEBS Lett. 298:297-300(1992) [PubMed: 1544464] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-18. Tissue: Liver.

Cross-references

Sequence databases
PIR	S62638.
3D structure databases
HSSP	HSSP built from PDB template 1EE2 based on UniProtKB P00328.
SMR	P25405. Positions 2-375.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P25405.
Family and domain databases
InterPro	IPR013154. AlcDHase_GroES-like. IPR002085. AlcDHase_SF_Zn. IPR013149. AlcDHase_Zn-bd. IPR002328. AlcDHase_Zn_CS. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProDom	P25405. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ADH1A_UROHA

Accession

Primary (citable) accession number: P25405

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	February 1, 1996
Last modified:	September 2, 2008
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)