P25390 (SSK22_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase SSK22
EC=2.7.11.1

Alternative name(s):
MAP kinase kinase kinase SSK22
Suppressor of sensor kinase 22

Gene names

Name:	SSK22
Ordered Locus Names:	YCR073C
ORF Names:	YCR73C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1331 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Activates the PBS2 MAP kinase kinase by phosphorylation.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with by SSK1.
Miscellaneous	Present with 56 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade involved in osmosensory signaling pathway Inferred from mutant phenotype Ref.4. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from sequence or structural similarity Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SSK1	Q07084	5	EBI-18129,EBI-18184

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1331	1331	Serine/threonine-protein kinase SSK22		PRO_0000086681
Regions
	Domain	1034 – 1310	277	Protein kinase
	Nucleotide binding	1040 – 1048	9	ATP By similarity
Sites
	Active site	1158	1	Proton acceptor By similarity
	Binding site	1063	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25390 [UniParc]. Last modified March 15, 2004. Version 2. Checksum: 2C3CE7C4FF4463B2	FASTA	1,331	152,718
	10 20 30 40 50 60 MMMDILNTQQ QKAAEGGRVL APHTISSKLV KRLSSHSSHK LSRSDLKALG GSETISDGPS 70 80 90 100 110 120 QLTFKDRYVF NESLYLKKLK KTALDDYYTR GIKLTNRYEE DDGDDEIIRL SNGDRIDEDL 130 140 150 160 170 180 HSGVKFFSTT PYCRKMRSDS DELAWNEIAT ERFKWQSMLA RVLKGDIVKG EKTRIANQVK 190 200 210 220 230 240 KPGLNKELSD EIWLELKAWL NGRTMQEMEQ SLTYLRDSSD SVFEEIMKFQ IPQGKILSLD 250 260 270 280 290 300 ALEAILQDLM NRYHSVVSYW PNLKKMYKDK PITNTAEFTA RIDVMNSWLN FKTNLTLRRQ 310 320 330 340 350 360 ELDDWINRFS PISSSDNCQE DFDGVPQWNC KMKILAEQLM KEKNIESIFQ KKIFYPLSPW 370 380 390 400 410 420 MFKLKLHFIV YRETLTKMNI KYPYERLRSL LAFPVYLIKE VILTRLSYAR KLKNPTMMMI 430 440 450 460 470 480 DQMIDDFNAF IRLSVQLKYT LTKYCSNLPF DVDFDPTFEN TVIEAIRYLF FLLNLKLIDS 490 500 510 520 530 540 SKQNFKAPDL LLKYWDHLKN TGHYINGAET VIPNEFLKLT LRLVHKLQFY LLKQQNFPPT 550 560 570 580 590 600 FANASEAEKW LSSIFENLGA MKRKLNRFSN ILVKAFQNSA VYQINHNAQL VKKLKDAHYF 610 620 630 640 650 660 LVYSGNTFES SGVYMFAAPE LLGCDNDTIL RILRNKSIGC DLVPKLDIGN NLNVYDITTK 670 680 690 700 710 720 ETDLNILVSK GEDSKGIPYY RVVANSSSDL DRHAHQSKKK NFSTDPFDQH LDEKNNEVFE 730 740 750 760 770 780 LEVALSSLGA LVVLYPGEPV VWDGPVYKLP GNNLFASNEM DLGKIGNPNT LILLNQGSNY 790 800 810 820 830 840 ALTYQIDKFN QTVGDSVSFI EKRCSLNSIE SSLQKINKAY YKLTYTVLNN YKGILGSFMK 850 860 870 880 890 900 QCPGNELLNS IFMFGRDFGR SFLKYNAFSS KRKYVIIFLM VKLGMNWLKF LVEECDPTDQ 910 920 930 940 950 960 RTFRWCVLAM DFAMQMTSGY NILALNVKQF QELKERVSVC MSLLISHFDV MGARATEAEN 970 980 990 1000 1010 1020 GMQQARLNID TEENIDEEAT LEINSRLRLE AIKTLEKTMK RNPRQMGKVL DATDQGNKYL 1030 1040 1050 1060 1070 1080 LSLASSLSNV SMRWQKRSFI GGGTFGQVYS AINLENGEIL AVKEIKIHDT TTMKKIFPLI 1090 1100 1110 1120 1130 1140 KEEMTVLEML NHPNIVQYYG VEVHRDKVNI FMEYCEGGSL ASLLDHGRIE DEMVTQVYTF 1150 1160 1170 1180 1190 1200 ELLEGLAYLH QSGVVHRDIK PENILLDFNG IIKYVDFGTA RTVVGSRTRT VRNAAVQDFG 1210 1220 1230 1240 1250 1260 VETKSLNEMM GTPMYMAPET ISGSAVKGKL GADDVWALGC VVLEMATGRR PWSNLDNEWA 1270 1280 1290 1300 1310 1320 IMYHVAAGRI PQLPNRDEMT AAGRAFLERC LVQDPTMRAT AVELLIDPWM IQIREIAFGN 1330 SEKDQVPILS S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Valles G., Volckaerts G. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor." Maeda T., Takekawa M., Saito H. Science 269:554-558(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59720 Genomic DNA. Translation: CAA42271.2. BK006937 Genomic DNA. Translation: DAA07544.1.
PIR	S19488.
RefSeq	NP_009998.2. NM_001178783.1.
3D structure databases
ProteinModelPortal	P25390.
SMR	P25390. Positions 973-1310.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5989N.
IntAct	P25390. 11 interactions.
MINT	MINT-592715.
STRING	4932.YCR073C.
Proteomic databases
PaxDb	P25390.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCR073C; YCR073C; YCR073C.
GeneID	850436.
KEGG	sce:YCR073C.
Organism-specific databases
CYGD	YCR073c.
SGD	S000000669. SSK22.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00590000083157.
HOGENOM	HOG000207441.
KO	K11230.
OMA	HILENGS.
OrthoDB	EOG4GF6P7.
Gene expression databases
Genevestigator	P25390.
GermOnline	YCR073C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR017240. MAPKKK_SSK22. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF037579. MAPKKK_SSK22. 1 hit.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966030.

Entry information

Entry name

SSK22_YEAST

Accession

Primary (citable) accession number: P25390
Secondary accession number(s): D6VR75

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	March 15, 2004
Last modified:	May 1, 2013
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program