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Protein

Serine/threonine-protein kinase SSK22

Gene

SSK22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Activates the PBS2 MAP kinase kinase by phosphorylation.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1063 – 10631ATPPROSITE-ProRule annotation
Active sitei1158 – 11581Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1040 – 10489ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase kinase kinase activity Source: SGD

GO - Biological processi

  1. activation of MAPKK activity Source: GOC
  2. MAPK cascade involved in osmosensory signaling pathway Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29373-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase SSK22 (EC:2.7.11.1)
Alternative name(s):
MAP kinase kinase kinase SSK22
Suppressor of sensor kinase 22
Gene namesi
Name:SSK22
Ordered Locus Names:YCR073C
ORF Names:YCR73C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome III

Organism-specific databases

CYGDiYCR073c.
SGDiS000000669. SSK22.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13311331Serine/threonine-protein kinase SSK22PRO_0000086681Add
BLAST

Proteomic databases

PaxDbiP25390.
PRIDEiP25390.

Expressioni

Gene expression databases

GenevestigatoriP25390.

Interactioni

Subunit structurei

Interacts with by SSK1.

Binary interactionsi

WithEntry#Exp.IntActNotes
SSK1Q070844EBI-18129,EBI-18184

Protein-protein interaction databases

BioGridi31048. 56 interactions.
DIPiDIP-5989N.
IntActiP25390. 10 interactions.
MINTiMINT-592715.
STRINGi4932.YCR073C.

Structurei

3D structure databases

ProteinModelPortaliP25390.
SMRiP25390. Positions 973-1310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1034 – 1310277Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00590000083157.
HOGENOMiHOG000207441.
InParanoidiP25390.
KOiK11230.
OMAiHANINGA.
OrthoDBiEOG7ZPNTB.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR017240. MAPKKK_SSK22.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037579. MAPKKK_SSK22. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25390-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMMDILNTQQ QKAAEGGRVL APHTISSKLV KRLSSHSSHK LSRSDLKALG
60 70 80 90 100
GSETISDGPS QLTFKDRYVF NESLYLKKLK KTALDDYYTR GIKLTNRYEE
110 120 130 140 150
DDGDDEIIRL SNGDRIDEDL HSGVKFFSTT PYCRKMRSDS DELAWNEIAT
160 170 180 190 200
ERFKWQSMLA RVLKGDIVKG EKTRIANQVK KPGLNKELSD EIWLELKAWL
210 220 230 240 250
NGRTMQEMEQ SLTYLRDSSD SVFEEIMKFQ IPQGKILSLD ALEAILQDLM
260 270 280 290 300
NRYHSVVSYW PNLKKMYKDK PITNTAEFTA RIDVMNSWLN FKTNLTLRRQ
310 320 330 340 350
ELDDWINRFS PISSSDNCQE DFDGVPQWNC KMKILAEQLM KEKNIESIFQ
360 370 380 390 400
KKIFYPLSPW MFKLKLHFIV YRETLTKMNI KYPYERLRSL LAFPVYLIKE
410 420 430 440 450
VILTRLSYAR KLKNPTMMMI DQMIDDFNAF IRLSVQLKYT LTKYCSNLPF
460 470 480 490 500
DVDFDPTFEN TVIEAIRYLF FLLNLKLIDS SKQNFKAPDL LLKYWDHLKN
510 520 530 540 550
TGHYINGAET VIPNEFLKLT LRLVHKLQFY LLKQQNFPPT FANASEAEKW
560 570 580 590 600
LSSIFENLGA MKRKLNRFSN ILVKAFQNSA VYQINHNAQL VKKLKDAHYF
610 620 630 640 650
LVYSGNTFES SGVYMFAAPE LLGCDNDTIL RILRNKSIGC DLVPKLDIGN
660 670 680 690 700
NLNVYDITTK ETDLNILVSK GEDSKGIPYY RVVANSSSDL DRHAHQSKKK
710 720 730 740 750
NFSTDPFDQH LDEKNNEVFE LEVALSSLGA LVVLYPGEPV VWDGPVYKLP
760 770 780 790 800
GNNLFASNEM DLGKIGNPNT LILLNQGSNY ALTYQIDKFN QTVGDSVSFI
810 820 830 840 850
EKRCSLNSIE SSLQKINKAY YKLTYTVLNN YKGILGSFMK QCPGNELLNS
860 870 880 890 900
IFMFGRDFGR SFLKYNAFSS KRKYVIIFLM VKLGMNWLKF LVEECDPTDQ
910 920 930 940 950
RTFRWCVLAM DFAMQMTSGY NILALNVKQF QELKERVSVC MSLLISHFDV
960 970 980 990 1000
MGARATEAEN GMQQARLNID TEENIDEEAT LEINSRLRLE AIKTLEKTMK
1010 1020 1030 1040 1050
RNPRQMGKVL DATDQGNKYL LSLASSLSNV SMRWQKRSFI GGGTFGQVYS
1060 1070 1080 1090 1100
AINLENGEIL AVKEIKIHDT TTMKKIFPLI KEEMTVLEML NHPNIVQYYG
1110 1120 1130 1140 1150
VEVHRDKVNI FMEYCEGGSL ASLLDHGRIE DEMVTQVYTF ELLEGLAYLH
1160 1170 1180 1190 1200
QSGVVHRDIK PENILLDFNG IIKYVDFGTA RTVVGSRTRT VRNAAVQDFG
1210 1220 1230 1240 1250
VETKSLNEMM GTPMYMAPET ISGSAVKGKL GADDVWALGC VVLEMATGRR
1260 1270 1280 1290 1300
PWSNLDNEWA IMYHVAAGRI PQLPNRDEMT AAGRAFLERC LVQDPTMRAT
1310 1320 1330
AVELLIDPWM IQIREIAFGN SEKDQVPILS S
Length:1,331
Mass (Da):152,718
Last modified:March 15, 2004 - v2
Checksum:i2C3CE7C4FF4463B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42271.2.
BK006937 Genomic DNA. Translation: DAA07544.1.
PIRiS19488.
RefSeqiNP_009998.2. NM_001178783.1.

Genome annotation databases

EnsemblFungiiYCR073C; YCR073C; YCR073C.
GeneIDi850436.
KEGGisce:YCR073C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42271.2.
BK006937 Genomic DNA. Translation: DAA07544.1.
PIRiS19488.
RefSeqiNP_009998.2. NM_001178783.1.

3D structure databases

ProteinModelPortaliP25390.
SMRiP25390. Positions 973-1310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31048. 56 interactions.
DIPiDIP-5989N.
IntActiP25390. 10 interactions.
MINTiMINT-592715.
STRINGi4932.YCR073C.

Proteomic databases

PaxDbiP25390.
PRIDEiP25390.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYCR073C; YCR073C; YCR073C.
GeneIDi850436.
KEGGisce:YCR073C.

Organism-specific databases

CYGDiYCR073c.
SGDiS000000669. SSK22.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00590000083157.
HOGENOMiHOG000207441.
InParanoidiP25390.
KOiK11230.
OMAiHANINGA.
OrthoDBiEOG7ZPNTB.

Enzyme and pathway databases

BioCyciYEAST:G3O-29373-MONOMER.

Miscellaneous databases

NextBioi966030.

Gene expression databases

GenevestigatoriP25390.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR017240. MAPKKK_SSK22.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037579. MAPKKK_SSK22. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Valles G., Volckaerts G.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor."
    Maeda T., Takekawa M., Saito H.
    Science 269:554-558(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSSK22_YEAST
AccessioniPrimary (citable) accession number: P25390
Secondary accession number(s): D6VR75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: March 15, 2004
Last modified: January 7, 2015
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 56 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.