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P25390 (SSK22_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase SSK22

EC=2.7.11.1
Alternative name(s):
MAP kinase kinase kinase SSK22
Suppressor of sensor kinase 22
Gene names
Name:SSK22
Ordered Locus Names:YCR073C
ORF Names:YCR73C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1331 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Activates the PBS2 MAP kinase kinase by phosphorylation.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with by SSK1.

Miscellaneous

Present with 56 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SSK1Q070844EBI-18129,EBI-18184

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13311331Serine/threonine-protein kinase SSK22
PRO_0000086681

Regions

Domain1034 – 1310277Protein kinase
Nucleotide binding1040 – 10489ATP By similarity

Sites

Active site11581Proton acceptor By similarity
Binding site10631ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P25390 [UniParc].

Last modified March 15, 2004. Version 2.
Checksum: 2C3CE7C4FF4463B2

FASTA1,331152,718
        10         20         30         40         50         60 
MMMDILNTQQ QKAAEGGRVL APHTISSKLV KRLSSHSSHK LSRSDLKALG GSETISDGPS 

        70         80         90        100        110        120 
QLTFKDRYVF NESLYLKKLK KTALDDYYTR GIKLTNRYEE DDGDDEIIRL SNGDRIDEDL 

       130        140        150        160        170        180 
HSGVKFFSTT PYCRKMRSDS DELAWNEIAT ERFKWQSMLA RVLKGDIVKG EKTRIANQVK 

       190        200        210        220        230        240 
KPGLNKELSD EIWLELKAWL NGRTMQEMEQ SLTYLRDSSD SVFEEIMKFQ IPQGKILSLD 

       250        260        270        280        290        300 
ALEAILQDLM NRYHSVVSYW PNLKKMYKDK PITNTAEFTA RIDVMNSWLN FKTNLTLRRQ 

       310        320        330        340        350        360 
ELDDWINRFS PISSSDNCQE DFDGVPQWNC KMKILAEQLM KEKNIESIFQ KKIFYPLSPW 

       370        380        390        400        410        420 
MFKLKLHFIV YRETLTKMNI KYPYERLRSL LAFPVYLIKE VILTRLSYAR KLKNPTMMMI 

       430        440        450        460        470        480 
DQMIDDFNAF IRLSVQLKYT LTKYCSNLPF DVDFDPTFEN TVIEAIRYLF FLLNLKLIDS 

       490        500        510        520        530        540 
SKQNFKAPDL LLKYWDHLKN TGHYINGAET VIPNEFLKLT LRLVHKLQFY LLKQQNFPPT 

       550        560        570        580        590        600 
FANASEAEKW LSSIFENLGA MKRKLNRFSN ILVKAFQNSA VYQINHNAQL VKKLKDAHYF 

       610        620        630        640        650        660 
LVYSGNTFES SGVYMFAAPE LLGCDNDTIL RILRNKSIGC DLVPKLDIGN NLNVYDITTK 

       670        680        690        700        710        720 
ETDLNILVSK GEDSKGIPYY RVVANSSSDL DRHAHQSKKK NFSTDPFDQH LDEKNNEVFE 

       730        740        750        760        770        780 
LEVALSSLGA LVVLYPGEPV VWDGPVYKLP GNNLFASNEM DLGKIGNPNT LILLNQGSNY 

       790        800        810        820        830        840 
ALTYQIDKFN QTVGDSVSFI EKRCSLNSIE SSLQKINKAY YKLTYTVLNN YKGILGSFMK 

       850        860        870        880        890        900 
QCPGNELLNS IFMFGRDFGR SFLKYNAFSS KRKYVIIFLM VKLGMNWLKF LVEECDPTDQ 

       910        920        930        940        950        960 
RTFRWCVLAM DFAMQMTSGY NILALNVKQF QELKERVSVC MSLLISHFDV MGARATEAEN 

       970        980        990       1000       1010       1020 
GMQQARLNID TEENIDEEAT LEINSRLRLE AIKTLEKTMK RNPRQMGKVL DATDQGNKYL 

      1030       1040       1050       1060       1070       1080 
LSLASSLSNV SMRWQKRSFI GGGTFGQVYS AINLENGEIL AVKEIKIHDT TTMKKIFPLI 

      1090       1100       1110       1120       1130       1140 
KEEMTVLEML NHPNIVQYYG VEVHRDKVNI FMEYCEGGSL ASLLDHGRIE DEMVTQVYTF 

      1150       1160       1170       1180       1190       1200 
ELLEGLAYLH QSGVVHRDIK PENILLDFNG IIKYVDFGTA RTVVGSRTRT VRNAAVQDFG 

      1210       1220       1230       1240       1250       1260 
VETKSLNEMM GTPMYMAPET ISGSAVKGKL GADDVWALGC VVLEMATGRR PWSNLDNEWA 

      1270       1280       1290       1300       1310       1320 
IMYHVAAGRI PQLPNRDEMT AAGRAFLERC LVQDPTMRAT AVELLIDPWM IQIREIAFGN 

      1330 
SEKDQVPILS S 

« Hide

References

« Hide 'large scale' references
[1]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Valles G., Volckaerts G.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor."
Maeda T., Takekawa M., Saito H.
Science 269:554-558(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59720 Genomic DNA. Translation: CAA42271.2.
BK006937 Genomic DNA. Translation: DAA07544.1.
PIRS19488.
RefSeqNP_009998.2. NM_001178783.1.

3D structure databases

ProteinModelPortalP25390.
SMRP25390. Positions 973-1310.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31048. 55 interactions.
DIPDIP-5989N.
IntActP25390. 10 interactions.
MINTMINT-592715.
STRING4932.YCR073C.

Proteomic databases

PaxDbP25390.
PRIDEP25390.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCR073C; YCR073C; YCR073C.
GeneID850436.
KEGGsce:YCR073C.

Organism-specific databases

CYGDYCR073c.
SGDS000000669. SSK22.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00590000083157.
HOGENOMHOG000207441.
KOK11230.
OMAHANINGA.
OrthoDBEOG7ZPNTB.

Enzyme and pathway databases

BioCycYEAST:G3O-29373-MONOMER.

Gene expression databases

GenevestigatorP25390.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR017240. MAPKKK_SSK22.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFPIRSF037579. MAPKKK_SSK22. 1 hit.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966030.

Entry information

Entry nameSSK22_YEAST
AccessionPrimary (citable) accession number: P25390
Secondary accession number(s): D6VR75
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: March 15, 2004
Last modified: June 11, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families