Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P25385 (BOS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein transport protein BOS1
Alternative name(s):
Bet one suppressor 1
Gene names
Name:BOS1
Ordered Locus Names:YLR078C
ORF Names:L9449.9
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length244 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

SNARE required for targeting and fusion of ER-derived transport vesicles with the Golgi complex. Ref.4

Subunit structure

Component of a SNARE complex consisting of SED5, BOS1, BET1 and SEC22 or YKT6. Interacts with YIF1 and YIP1. Ref.4 Ref.5

Subcellular location

Golgi apparatus membrane; Single-pass type IV membrane protein Probable. Endoplasmic reticulum membrane; Single-pass type IV membrane protein Probable.

Sequence similarities

Belongs to the BOS1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 244244Protein transport protein BOS1
PRO_0000207557

Regions

Topological domain1 – 222222Cytoplasmic Potential
Transmembrane223 – 24018Helical; Anchor for type IV membrane protein; Potential
Topological domain241 – 2444Vesicular Potential

Amino acid modifications

Modified residue11N-acetylmethionine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P25385 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: E2F13D444ADC0D93

FASTA24427,500
        10         20         30         40         50         60 
MNALYNHAVK QKNQLQQELA RFEKNSVTAP ISLQGSISAT LVSLEKTVKQ YAEHLNRYKE 

        70         80         90        100        110        120 
DTNAEEIDPK FANRLATLTQ DLHDFTAKFK DLKQSYNENN SRTQLFGSGA SHVMDSDNPF 

       130        140        150        160        170        180 
STSETIMNKR NVGGASANGK EGSSNGGGLP LYQGLQKEQS VFERGNAQLD YILEMGQQSF 

       190        200        210        220        230        240 
ENIVEQNKIL SKVQDRMSNG LRTLGVSEQT ITSINKRVFK DKLVFWIALI LLIIGIYYVL 


KWLR 

« Hide

References

« Hide 'large scale' references
[1]"The BOS1 gene encodes an essential 27-kD putative membrane protein that is required for vesicular transport from the ER to the Golgi complex in yeast."
Shim J., Newman A.P., Ferro-Novick S.
J. Cell Biol. 113:55-64(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Topological restriction of SNARE-dependent membrane fusion."
Parlati F., McNew J.A., Fukuda R., Miller R., Sollner T.H., Rothman J.E.
Nature 407:194-198(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH BET1; SEC22 AND SED5.
[5]"The Yip1p.Yif1p complex is required for the fusion competence of endoplasmic reticulum-derived vesicles."
Barrowman J., Wang W., Zhang Y., Ferro-Novick S.
J. Biol. Chem. 278:19878-19884(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIF1 AND YIP1.
[6]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X57792 Genomic DNA. Translation: CAA40933.1.
Z73250 Genomic DNA. Translation: CAA97636.1.
U53880 Genomic DNA. Translation: AAB67582.1.
BK006945 Genomic DNA. Translation: DAA09394.1.
PIRA39206.
RefSeqNP_013179.1. NM_001181965.1.

3D structure databases

ProteinModelPortalP25385.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31351. 146 interactions.
DIPDIP-2020N.
IntActP25385. 10 interactions.
MINTMINT-391154.
STRING4932.YLR078C.

Proteomic databases

MaxQBP25385.
PaxDbP25385.
PeptideAtlasP25385.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR078C; YLR078C; YLR078C.
GeneID850767.
KEGGsce:YLR078C.

Organism-specific databases

CYGDYLR078c.
SGDS000004068. BOS1.

Phylogenomic databases

eggNOGNOG246137.
HOGENOMHOG000217458.
KOK08496.
OMATSETIMN.
OrthoDBEOG7X0VV3.

Enzyme and pathway databases

BioCycYEAST:G3O-32229-MONOMER.

Gene expression databases

GenevestigatorP25385.

Family and domain databases

ProtoNetSearch...

Other

NextBio966927.
PROP25385.

Entry information

Entry nameBOS1_YEAST
AccessionPrimary (citable) accession number: P25385
Secondary accession number(s): D6VY78
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 14, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families