Protein transport protein BOS1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P25385 (BOS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein transport protein BOS1

Alternative name(s):
Bet one suppressor 1

Gene names

Name:	BOS1
Ordered Locus Names:	YLR078C
ORF Names:	L9449.9

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	SNARE required for targeting and fusion of ER-derived transport vesicles with the Golgi complex. Ref.4
Subunit structure	Component of a SNARE complex consisting of SED5, BOS1, BET1 and SEC22 or YKT6. Interacts with YIF1 and YIP1. Ref.4 Ref.5
Subcellular location	Golgi apparatus membrane; Single-pass type IV membrane protein Probable. Endoplasmic reticulum membrane; Single-pass type IV membrane protein Probable.
Sequence similarities	Belongs to the BOS1 family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype PubMed 8500167. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle fusion with Golgi apparatus Inferred from mutant phenotype PubMed 8500167. Source: SGD
Cellular_component	ER to Golgi transport vesicle membrane Inferred from direct assay PubMed 8500167. Source: SGD Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell SNARE complex Inferred from direct assay Ref.4. Source: SGD endoplasmic reticulum membrane Inferred from direct assay PubMed 1396561. Source: SGD integral to membrane Inferred from direct assay PubMed 1396561. Source: SGD
Molecular_function	SNAP receptor activity Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 244

244

Protein transport protein BOS1

PRO_0000207557

Regions

Topological domain

1 – 222

222

Cytoplasmic Potential

Transmembrane

223 – 240

Helical; Anchor for type IV membrane protein; Potential

Topological domain

241 – 244

Vesicular Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P25385 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: E2F13D444ADC0D93
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FASTA

244

27,500

        10         20         30         40         50         60 
MNALYNHAVK QKNQLQQELA RFEKNSVTAP ISLQGSISAT LVSLEKTVKQ YAEHLNRYKE 

        70         80         90        100        110        120 
DTNAEEIDPK FANRLATLTQ DLHDFTAKFK DLKQSYNENN SRTQLFGSGA SHVMDSDNPF 

       130        140        150        160        170        180 
STSETIMNKR NVGGASANGK EGSSNGGGLP LYQGLQKEQS VFERGNAQLD YILEMGQQSF 

       190        200        210        220        230        240 
ENIVEQNKIL SKVQDRMSNG LRTLGVSEQT ITSINKRVFK DKLVFWIALI LLIIGIYYVL 


KWLR

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The BOS1 gene encodes an essential 27-kD putative membrane protein that is required for vesicular transport from the ER to the Golgi complex in yeast." Shim J., Newman A.P., Ferro-Novick S. J. Cell Biol. 113:55-64(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Topological restriction of SNARE-dependent membrane fusion." Parlati F., McNew J.A., Fukuda R., Miller R., Sollner T.H., Rothman J.E. Nature 407:194-198(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH BET1; SEC22 AND SED5.
[5]	"The Yip1p.Yif1p complex is required for the fusion competence of endoplasmic reticulum-derived vesicles." Barrowman J., Wang W., Zhang Y., Ferro-Novick S. J. Biol. Chem. 278:19878-19884(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH YIF1 AND YIP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57792 Genomic DNA. Translation: CAA40933.1. Z73250 Genomic DNA. Translation: CAA97636.1. U53880 Genomic DNA. Translation: AAB67582.1. BK006945 Genomic DNA. Translation: DAA09394.1.
PIR	A39206.
RefSeq	NP_013179.1. NM_001181965.1.
3D structure databases
ProteinModelPortal	P25385.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2020N.
IntAct	P25385. 10 interactions.
MINT	MINT-391154.
STRING	4932.YLR078C.
Proteomic databases
PaxDb	P25385.
PeptideAtlas	P25385.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR078C; YLR078C; YLR078C.
GeneID	850767.
KEGG	sce:YLR078C.
Organism-specific databases
CYGD	YLR078c.
SGD	S000004068. BOS1.
Phylogenomic databases
eggNOG	NOG246137.
HOGENOM	HOG000217458.
KO	K08496.
OMA	AKQDKWL.
OrthoDB	EOG4W3WXH.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32229-MONOMER.
Gene expression databases
Genevestigator	P25385.
GermOnline	YLR078C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	966927.

Entry information

Entry name

BOS1_YEAST

Accession

Primary (citable) accession number: P25385
Secondary accession number(s): D6VY78

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 29, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents