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P25383

- YC21A_YEAST

UniProt

P25383 - YC21A_YEAST

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Protein

Transposon Ty2-C Gag polyprotein

Gene

TY2A-C

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Capsid protein (CA) is the structural component of the virus-like particle (VLP), forming the shell that encapsulates the retrotransposons dimeric RNA genome. The particles are assembled from trimer-clustered units and there are holes in the capsid shells that allow for the diffusion of macromolecules. CA has also nucleocapsid-like chaperone activity, promoting primer tRNA(i)-Met annealing to the multipartite primer-binding site (PBS), dimerization of Ty2 RNA and initiation of reverse transcription (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei397 – 3982Cleavage; by Ty2 proteaseBy similarity

GO - Molecular functioni

  1. RNA binding Source: SGD

GO - Biological processi

  1. transposition, RNA-mediated Source: SGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Transposon Ty2-C Gag polyprotein
Alternative name(s):
Transposon Ty2 protein A
Short name:
TY2A
Short name:
TYA
Ty1-17 protein A
Cleaved into the following 2 chains:
Capsid protein
Short name:
CA
Gene namesi
Name:TY2A-C
Synonyms:YCLWTy2-1 GAG
Ordered Locus Names:YCL020W
ORF Names:YCL20W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCL020w.
SGDiS000000525. YCL020W.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. retrotransposon nucleocapsid Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438Transposon Ty2-C Gag polyproteinPRO_0000203495Add
BLAST
Chaini1 – 397397Capsid proteinBy similarityPRO_0000279282Add
BLAST
Peptidei398 – 43841Gag-p4By similarityPRO_0000279283Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP25383.

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

BioGridi30963. 7 interactions.
DIPiDIP-7334N.
IntActiP25383. 4 interactions.
MINTiMINT-406398.
STRINGi4932.YCL020W.

Structurei

3D structure databases

ProteinModelPortaliP25383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni295 – 397103RNA-bindingBy similarityAdd
BLAST

Domaini

The C-terminal RNA-binding region of CA is sufficient for all its nucleocapsid-like chaperone activities.By similarity

Phylogenomic databases

GeneTreeiENSGT00730000111602.
HOGENOMiHOG000000740.
InParanoidiP25383.
OrthoDBiEOG7TJ3S3.

Family and domain databases

InterProiIPR015820. Retrotransposon_Ty1A_N.
[Graphical view]
PfamiPF01021. TYA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Note: The Gag-Pol polyprotein is generated by a +1 ribosomal frameshift.

Isoform Transposon Ty2-C Gag polyprotein (identifier: P25383-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESQQLHQNP RSLHGSAYAS VTSKEVPSNQ DPLAVSASNL PEFDRDSTKV
60 70 80 90 100
NSQQETTPGT SAVPENHHHV SPQPASVPPP QNGQYQQHGM MTPNKAMASN
110 120 130 140 150
WAHYQQPSMM TCSHYQTSPA YYQPDPHYPL PQYIPPLSTS SPDPIDSQNQ
160 170 180 190 200
HSEVPQAETK VRNNVLPPHT LTSEENFSTW VKFYIRFLKN SNLGDIIPND
210 220 230 240 250
QGEIKRQMTY EEHAYIYNTF QAFAPFHLLP TWVKQILEIN YADILTVLCK
260 270 280 290 300
SVSKMQTNNQ ELKDWIALAN LEYDGSTSAD TFEITVSTII QRLKENNINV
310 320 330 340 350
SDRLACQLIL KGLSGDFKYL RNQYRTKTNM KLSQLFAEIQ LIYDENKIMN
360 370 380 390 400
LNKPSQYKQH SEYKNVSRTS PNTTNTKVTT RNYQRTNSSK PRAAKAHNIA
410 420 430
TSSKFSRVNN DHINESTVSS QYLSDDNELS LRPATERI

Note: Produced by conventional translation.

Length:438
Mass (Da):49,841
Last modified:September 26, 2001 - v2
Checksum:iD92E6A7BDA32A5B4
GO
Isoform Transposon Ty2-C Gag-Pol polyprotein (identifier: P25384-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P25384.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by +1 ribosomal frameshifting between codon Leu-431 and Gly-432 of the YCL020W ORF.

Length:1,770
Mass (Da):202,096
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti174 – 1741E → D(PubMed:3010239)Curated
Sequence conflicti174 – 1741E → D in CAA27457. (PubMed:2997719)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03840 Genomic DNA. Translation: CAA27457.1.
X59720 Genomic DNA. Translation: CAA42364.2.
X02546 Genomic DNA. Translation: CAA26398.1.
X00394 Genomic DNA. Translation: CAA25113.1.
BK006937 Genomic DNA. Translation: DAA07464.1.
PIRiS19347.
RefSeqiNP_009910.2. NM_001178667.1. [P25383-1]

Genome annotation databases

EnsemblFungiiYCL020W; YCL020W; YCL020W. [P25383-1]
GeneIDi850339.
KEGGisce:YCL020W.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03840 Genomic DNA. Translation: CAA27457.1 .
X59720 Genomic DNA. Translation: CAA42364.2 .
X02546 Genomic DNA. Translation: CAA26398.1 .
X00394 Genomic DNA. Translation: CAA25113.1 .
BK006937 Genomic DNA. Translation: DAA07464.1 .
PIRi S19347.
RefSeqi NP_009910.2. NM_001178667.1. [P25383-1 ]

3D structure databases

ProteinModelPortali P25383.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 30963. 7 interactions.
DIPi DIP-7334N.
IntActi P25383. 4 interactions.
MINTi MINT-406398.
STRINGi 4932.YCL020W.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCL020W ; YCL020W ; YCL020W . [P25383-1 ]
GeneIDi 850339.
KEGGi sce:YCL020W.

Organism-specific databases

CYGDi YCL020w.
SGDi S000000525. YCL020W.

Phylogenomic databases

GeneTreei ENSGT00730000111602.
HOGENOMi HOG000000740.
InParanoidi P25383.
OrthoDBi EOG7TJ3S3.

Miscellaneous databases

NextBioi 965780.

Gene expression databases

Genevestigatori P25383.

Family and domain databases

InterProi IPR015820. Retrotransposon_Ty1A_N.
[Graphical view ]
Pfami PF01021. TYA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A 'hot-spot' for Ty transposition on the left arm of yeast chromosome III."
    Warmington J.R., Anwar R., Newlon C.S., Waring R.B., Davies R.W., Indge K.J., Oliver S.G.
    Nucleic Acids Res. 14:3475-3485(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence characterization of Ty 1-17, a class II transposon from yeast."
    Warmington J.R., Waring R.B., Newlon C.S., Indge K.J., Oliver S.G.
    Nucleic Acids Res. 13:6679-6693(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Variants within the yeast Ty sequence family encode a class of structurally conserved proteins."
    Fulton A.M., Mellor J., Dobson M.J., Chester J., Warmington J.R., Indge K.J., Oliver S.G., de la Paz P., Wilson W., Kingsman A.J., Kingsman S.M.
    Nucleic Acids Res. 13:4097-4112(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MD 40-4C.
  4. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Gromadka R.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
  8. "Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence."
    Kim J.M., Vanguri S., Boeke J.D., Gabriel A., Voytas D.F.
    Genome Res. 8:464-478(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.
  9. "Happy together: the life and times of Ty retrotransposons and their hosts."
    Lesage P., Todeschini A.L.
    Cytogenet. Genome Res. 110:70-90(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiYC21A_YEAST
AccessioniPrimary (citable) accession number: P25383
Secondary accession number(s): D6VQZ5, Q07125
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: September 26, 2001
Last modified: October 29, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Retrotransposons are mobile genetic entities that are able to replicate via an RNA intermediate and a reverse transcription step. In contrast to retroviruses, retrotransposons are non-infectious, lack an envelope and remain intracellular. Ty2 retrotransposons belong to the copia elements (pseudoviridae).

Keywords - Technical termi

Complete proteome, Reference proteome, Transposable element

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3