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Protein

Transposon Ty2-C Gag polyprotein

Gene

TY2A-C

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Capsid protein (CA) is the structural component of the virus-like particle (VLP), forming the shell that encapsulates the retrotransposons dimeric RNA genome. The particles are assembled from trimer-clustered units and there are holes in the capsid shells that allow for the diffusion of macromolecules. CA has also nucleocapsid-like chaperone activity, promoting primer tRNA(i)-Met annealing to the multipartite primer-binding site (PBS), dimerization of Ty2 RNA and initiation of reverse transcription (By similarity).By similarity

GO - Molecular functioni

  • RNA binding Source: SGD

GO - Biological processi

  • transposition, RNA-mediated Source: SGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Transposon Ty2-C Gag polyprotein
Alternative name(s):
Transposon Ty2 protein A
Short name:
TY2A
Short name:
TYA
Ty1-17 protein A
Cleaved into the following 2 chains:
Capsid protein
Short name:
CA
Gene namesi
Name:TY2A-C
Synonyms:YCLWTy2-1 GAG
Ordered Locus Names:YCL020W
ORF Names:YCL20W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL020W.
SGDiS000000525. YCL020W.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • retrotransposon nucleocapsid Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002034951 – 438Transposon Ty2-C Gag polyproteinAdd BLAST438
ChainiPRO_00002792821 – 397Capsid proteinBy similarityAdd BLAST397
PeptideiPRO_0000279283398 – 438Gag-p4By similarityAdd BLAST41

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei397 – 398Cleavage; by Ty2 proteaseBy similarity2

Proteomic databases

PRIDEiP25383.

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

BioGridi30963. 6 interactors.
DIPiDIP-7334N.
IntActiP25383. 4 interactors.
MINTiMINT-406398.

Structurei

3D structure databases

ProteinModelPortaliP25383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni295 – 397RNA-bindingBy similarityAdd BLAST103

Domaini

The C-terminal RNA-binding region of CA is sufficient for all its nucleocapsid-like chaperone activities.By similarity

Phylogenomic databases

GeneTreeiENSGT00860000134578.
HOGENOMiHOG000000740.
InParanoidiP25383.
OrthoDBiEOG092C2F0T.

Family and domain databases

InterProiIPR015820. Retrotransposon_Ty1A_N.
[Graphical view]
PfamiPF01021. TYA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Note: The Gag-Pol polyprotein is generated by a +1 ribosomal frameshift.
Isoform Transposon Ty2-C Gag polyprotein (identifier: P25383-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESQQLHQNP RSLHGSAYAS VTSKEVPSNQ DPLAVSASNL PEFDRDSTKV
60 70 80 90 100
NSQQETTPGT SAVPENHHHV SPQPASVPPP QNGQYQQHGM MTPNKAMASN
110 120 130 140 150
WAHYQQPSMM TCSHYQTSPA YYQPDPHYPL PQYIPPLSTS SPDPIDSQNQ
160 170 180 190 200
HSEVPQAETK VRNNVLPPHT LTSEENFSTW VKFYIRFLKN SNLGDIIPND
210 220 230 240 250
QGEIKRQMTY EEHAYIYNTF QAFAPFHLLP TWVKQILEIN YADILTVLCK
260 270 280 290 300
SVSKMQTNNQ ELKDWIALAN LEYDGSTSAD TFEITVSTII QRLKENNINV
310 320 330 340 350
SDRLACQLIL KGLSGDFKYL RNQYRTKTNM KLSQLFAEIQ LIYDENKIMN
360 370 380 390 400
LNKPSQYKQH SEYKNVSRTS PNTTNTKVTT RNYQRTNSSK PRAAKAHNIA
410 420 430
TSSKFSRVNN DHINESTVSS QYLSDDNELS LRPATERI
Note: Produced by conventional translation.
Length:438
Mass (Da):49,841
Last modified:September 26, 2001 - v2
Checksum:iD92E6A7BDA32A5B4
GO
Isoform Transposon Ty2-C Gag-Pol polyprotein (identifier: P25384-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P25384.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by +1 ribosomal frameshifting between codon Leu-431 and Gly-432 of the YCL020W ORF.
Length:1,770
Mass (Da):202,096
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti174E → D (PubMed:3010239).Curated1
Sequence conflicti174E → D in CAA27457 (PubMed:2997719).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03840 Genomic DNA. Translation: CAA27457.1.
X59720 Genomic DNA. Translation: CAA42364.2.
X02546 Genomic DNA. Translation: CAA26398.1.
X00394 Genomic DNA. Translation: CAA25113.1.
BK006937 Genomic DNA. Translation: DAA07464.1.
PIRiS19347.
RefSeqiNP_009910.2. NM_001178667.1. [P25383-1]

Genome annotation databases

EnsemblFungiiCAA42364; CAA42364; CAA42364.
YCL020W; YCL020W; YCL020W. [P25383-1]
GeneIDi850339.
KEGGisce:YCL020W.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03840 Genomic DNA. Translation: CAA27457.1.
X59720 Genomic DNA. Translation: CAA42364.2.
X02546 Genomic DNA. Translation: CAA26398.1.
X00394 Genomic DNA. Translation: CAA25113.1.
BK006937 Genomic DNA. Translation: DAA07464.1.
PIRiS19347.
RefSeqiNP_009910.2. NM_001178667.1. [P25383-1]

3D structure databases

ProteinModelPortaliP25383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30963. 6 interactors.
DIPiDIP-7334N.
IntActiP25383. 4 interactors.
MINTiMINT-406398.

Proteomic databases

PRIDEiP25383.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42364; CAA42364; CAA42364.
YCL020W; YCL020W; YCL020W. [P25383-1]
GeneIDi850339.
KEGGisce:YCL020W.

Organism-specific databases

EuPathDBiFungiDB:YCL020W.
SGDiS000000525. YCL020W.

Phylogenomic databases

GeneTreeiENSGT00860000134578.
HOGENOMiHOG000000740.
InParanoidiP25383.
OrthoDBiEOG092C2F0T.

Family and domain databases

InterProiIPR015820. Retrotransposon_Ty1A_N.
[Graphical view]
PfamiPF01021. TYA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYC21A_YEAST
AccessioniPrimary (citable) accession number: P25383
Secondary accession number(s): D6VQZ5, Q07125
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: September 26, 2001
Last modified: November 30, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Retrotransposons are mobile genetic entities that are able to replicate via an RNA intermediate and a reverse transcription step. In contrast to retroviruses, retrotransposons are non-infectious, lack an envelope and remain intracellular. Ty2 retrotransposons belong to the copia elements (pseudoviridae).

Keywords - Technical termi

Complete proteome, Reference proteome, Transposable element

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.