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P25382 (NLE1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome assembly protein 4
Gene names
Name:RSA4
Ordered Locus Names:YCR072C
ORF Names:YCR72C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length515 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in processing and efficient intra-nuclear transport or pre-60S ribosomal subunits. Forms a complex with REA1 which is essential for ATP-dependent dissociation of a group of nonribosomal factors from the pre-60S particle. Ref.6 Ref.10 Ref.11

Subunit structure

Associates with the pre-60S ribosomal particle. Interacts with MND1. Ref.10 Ref.11

Subcellular location

Nucleus › nucleolus Ref.6.

Sequence similarities

Belongs to the NLE1/RSA4 family.

Contains 8 WD repeats.

Ontologies

Keywords
   Biological processRibosome biogenesis
   Cellular componentNucleus
   DomainRepeat
WD repeat
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processribosomal large subunit assembly

Inferred from mutant phenotype Ref.6. Source: SGD

   Cellular_componentnucleolus

Inferred from direct assay Ref.6. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MDN1Q120196EBI-21980,EBI-10633

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 515515Ribosome assembly protein 4
PRO_0000051471

Regions

Repeat141 – 18141WD 1
Repeat184 – 22340WD 2
Repeat227 – 27347WD 3
Repeat276 – 31439WD 4
Repeat352 – 39645WD 5
Repeat400 – 43940WD 6
Repeat442 – 48140WD 7
Repeat484 – 51431WD 8

Amino acid modifications

Modified residue1371Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P25382 [UniParc].

Last modified June 27, 2003. Version 3.
Checksum: 04451B0C38FB5318

FASTA51557,026
        10         20         30         40         50         60 
MSTLIPPPSK KQKKEAQLPR EVAIIPKDLP NVSIKFQALD TGDNVGGALR VPGAISEKQL 

        70         80         90        100        110        120 
EELLNQLNGT SDDPVPYTFS CTIQGKKASD PVKTIDITDN LYSSLIKPGY NSTEDQITLL 

       130        140        150        160        170        180 
YTPRAVFKVK PVTRSSSAIA GHGSTILCSA FAPHTSSRMV TGAGDNTARI WDCDTQTPMH 

       190        200        210        220        230        240 
TLKGHYNWVL CVSWSPDGEV IATGSMDNTI RLWDPKSGQC LGDALRGHSK WITSLSWEPI 

       250        260        270        280        290        300 
HLVKPGSKPR LASSSKDGTI KIWDTVSRVC QYTMSGHTNS VSCVKWGGQG LLYSGSHDRT 

       310        320        330        340        350        360 
VRVWDINSQG RCINILKSHA HWVNHLSLST DYALRIGAFD HTGKKPSTPE EAQKKALENY 

       370        380        390        400        410        420 
EKICKKNGNS EEMMVTASDD YTMFLWNPLK STKPIARMTG HQKLVNHVAF SPDGRYIVSA 

       430        440        450        460        470        480 
SFDNSIKLWD GRDGKFISTF RGHVASVYQV AWSSDCRLLV SCSKDTTLKV WDVRTRKLSV 

       490        500        510 
DLPGHKDEVY TVDWSVDGKR VCSGGKDKMV RLWTH

« Hide

References

« Hide 'large scale' references
[1]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Jimenez A.
Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]Valles G., Volckaerts G.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 444.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The essential WD-repeat protein Rsa4p is required for rRNA processing and intra-nuclear transport of 60S ribosomal subunits."
de la Cruz J., Sanz-Martinez E., Remacha M.
Nucleic Acids Res. 33:5728-5739(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes."
Fleischer T.C., Weaver C.M., McAfee K.J., Jennings J.L., Link A.J.
Genes Dev. 20:1294-1307(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: COPURIFICATION WITH RIBOSOMAL COMPLEXES, MASS SPECTROMETRY.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, MASS SPECTROMETRY.
[9]"60S ribosomal subunit assembly dynamics defined by semi-quantitative mass spectrometry of purified complexes."
Lebreton A., Rousselle J.C., Lenormand P., Namane A., Jacquier A., Fromont-Racine M., Saveanu C.
Nucleic Acids Res. 36:4988-4999(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSOCIATION WITH THE PRE-60S PARTICLE.
[10]"Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunits."
Ulbrich C., Diepholz M., Bassler J., Kressler D., Pertschy B., Galani K., Bottcher B., Hurt E.
Cell 138:911-922(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ASSOCIATION WITH THE PRE-60S PARTICLE, INTERACTION WITH MDN1.
[11]"The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly."
Bassler J., Kallas M., Pertschy B., Ulbrich C., Thoms M., Hurt E.
Mol. Cell 38:712-721(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MDN1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X59720 Genomic DNA. Translation: CAC42989.1.
AY692666 Genomic DNA. Translation: AAT92685.1.
BK006937 Genomic DNA. Translation: DAA07543.1.
PIRS19487.
RefSeqNP_009997.2. NM_001178782.1.

3D structure databases

ProteinModelPortalP25382.
SMRP25382. Positions 66-514.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-5365N.
IntActP25382. 17 interactions.
MINTMINT-531710.
STRING4932.YCR072C.

Proteomic databases

PaxDbP25382.
PeptideAtlasP25382.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCR072C; YCR072C; YCR072C.
GeneID850435.
KEGGsce:YCR072C.

Organism-specific databases

CYGDYCR072c.
SGDS000000668. RSA4.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00690000102167.
KOK14855.
OMAHFTCKGH.
OrthoDBEOG4PP1R9.

Enzyme and pathway databases

BioCycYEAST:G3O-29372-MONOMER.

Gene expression databases

GenevestigatorP25382.
GermOnlineYCR072C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR012972. NLE.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF08154. NLE. 1 hit.
PF00400. WD40. 8 hits.
[Graphical view]
PRINTSPR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMSSF50978. WD40_like. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 5 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966027.

Entry information

Entry nameNLE1_YEAST
AccessionPrimary (citable) accession number: P25382
Secondary accession number(s): D6VR74, Q8NKJ4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 27, 2003
Last modified: May 29, 2013
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents