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P25382

- NLE1_YEAST

UniProt

P25382 - NLE1_YEAST

Protein

Ribosome assembly protein 4

Gene

RSA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 3 (27 Jun 2003)
      Previous versions | rss
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    Functioni

    Involved in processing and efficient intra-nuclear transport or pre-60S ribosomal subunits. Forms a complex with REA1 which is essential for ATP-dependent dissociation of a group of nonribosomal factors from the pre-60S particle.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. ribosomal large subunit assembly Source: SGD

    Keywords - Biological processi

    Ribosome biogenesis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29372-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosome assembly protein 4
    Gene namesi
    Name:RSA4
    Ordered Locus Names:YCR072C
    ORF Names:YCR72C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR072c.
    SGDiS000000668. RSA4.

    Subcellular locationi

    Nucleusnucleolus 1 Publication

    GO - Cellular componenti

    1. nucleolus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 515515Ribosome assembly protein 4PRO_0000051471Add
    BLAST

    Proteomic databases

    MaxQBiP25382.
    PaxDbiP25382.
    PeptideAtlasiP25382.

    Expressioni

    Gene expression databases

    GenevestigatoriP25382.

    Interactioni

    Subunit structurei

    Associates with the pre-60S ribosomal particle. Interacts with MND1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MDN1Q120196EBI-21980,EBI-10633

    Protein-protein interaction databases

    BioGridi31047. 47 interactions.
    DIPiDIP-5365N.
    IntActiP25382. 17 interactions.
    MINTiMINT-531710.
    STRINGi4932.YCR072C.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3J65electron microscopy8.70q1-515[»]
    ProteinModelPortaliP25382.
    SMRiP25382. Positions 94-513.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati141 – 18141WD 1Add
    BLAST
    Repeati184 – 22340WD 2Add
    BLAST
    Repeati227 – 27347WD 3Add
    BLAST
    Repeati276 – 31439WD 4Add
    BLAST
    Repeati352 – 39645WD 5Add
    BLAST
    Repeati400 – 43940WD 6Add
    BLAST
    Repeati442 – 48140WD 7Add
    BLAST
    Repeati484 – 51431WD 8Add
    BLAST

    Sequence similaritiesi

    Belongs to the NLE1/RSA4 family.Curated
    Contains 8 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00750000117266.
    KOiK14855.
    OMAiLTGHSKW.
    OrthoDBiEOG73Z348.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR012972. NLE.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF08154. NLE. 1 hit.
    PF00400. WD40. 8 hits.
    [Graphical view]
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 8 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 2 hits.
    PROSITEiPS00678. WD_REPEATS_1. 5 hits.
    PS50082. WD_REPEATS_2. 7 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25382-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTLIPPPSK KQKKEAQLPR EVAIIPKDLP NVSIKFQALD TGDNVGGALR    50
    VPGAISEKQL EELLNQLNGT SDDPVPYTFS CTIQGKKASD PVKTIDITDN 100
    LYSSLIKPGY NSTEDQITLL YTPRAVFKVK PVTRSSSAIA GHGSTILCSA 150
    FAPHTSSRMV TGAGDNTARI WDCDTQTPMH TLKGHYNWVL CVSWSPDGEV 200
    IATGSMDNTI RLWDPKSGQC LGDALRGHSK WITSLSWEPI HLVKPGSKPR 250
    LASSSKDGTI KIWDTVSRVC QYTMSGHTNS VSCVKWGGQG LLYSGSHDRT 300
    VRVWDINSQG RCINILKSHA HWVNHLSLST DYALRIGAFD HTGKKPSTPE 350
    EAQKKALENY EKICKKNGNS EEMMVTASDD YTMFLWNPLK STKPIARMTG 400
    HQKLVNHVAF SPDGRYIVSA SFDNSIKLWD GRDGKFISTF RGHVASVYQV 450
    AWSSDCRLLV SCSKDTTLKV WDVRTRKLSV DLPGHKDEVY TVDWSVDGKR 500
    VCSGGKDKMV RLWTH 515
    Length:515
    Mass (Da):57,026
    Last modified:June 27, 2003 - v3
    Checksum:i04451B0C38FB5318
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAC42989.1.
    AY692666 Genomic DNA. Translation: AAT92685.1.
    BK006937 Genomic DNA. Translation: DAA07543.1.
    PIRiS19487.
    RefSeqiNP_009997.2. NM_001178782.1.

    Genome annotation databases

    EnsemblFungiiYCR072C; YCR072C; YCR072C.
    GeneIDi850435.
    KEGGisce:YCR072C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAC42989.1 .
    AY692666 Genomic DNA. Translation: AAT92685.1 .
    BK006937 Genomic DNA. Translation: DAA07543.1 .
    PIRi S19487.
    RefSeqi NP_009997.2. NM_001178782.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3J65 electron microscopy 8.70 q 1-515 [» ]
    ProteinModelPortali P25382.
    SMRi P25382. Positions 94-513.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31047. 47 interactions.
    DIPi DIP-5365N.
    IntActi P25382. 17 interactions.
    MINTi MINT-531710.
    STRINGi 4932.YCR072C.

    Proteomic databases

    MaxQBi P25382.
    PaxDbi P25382.
    PeptideAtlasi P25382.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR072C ; YCR072C ; YCR072C .
    GeneIDi 850435.
    KEGGi sce:YCR072C.

    Organism-specific databases

    CYGDi YCR072c.
    SGDi S000000668. RSA4.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00750000117266.
    KOi K14855.
    OMAi LTGHSKW.
    OrthoDBi EOG73Z348.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29372-MONOMER.

    Miscellaneous databases

    NextBioi 966027.
    PROi P25382.

    Gene expression databases

    Genevestigatori P25382.

    Family and domain databases

    Gene3Di 2.130.10.10. 2 hits.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR012972. NLE.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF08154. NLE. 1 hit.
    PF00400. WD40. 8 hits.
    [Graphical view ]
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 8 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 2 hits.
    PROSITEi PS00678. WD_REPEATS_1. 5 hits.
    PS50082. WD_REPEATS_2. 7 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Jimenez A.
      Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. Valles G., Volckaerts G.
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 444.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "The essential WD-repeat protein Rsa4p is required for rRNA processing and intra-nuclear transport of 60S ribosomal subunits."
      de la Cruz J., Sanz-Martinez E., Remacha M.
      Nucleic Acids Res. 33:5728-5739(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes."
      Fleischer T.C., Weaver C.M., McAfee K.J., Jennings J.L., Link A.J.
      Genes Dev. 20:1294-1307(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: COPURIFICATION WITH RIBOSOMAL COMPLEXES, IDENTIFICATION BY MASS SPECTROMETRY.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "60S ribosomal subunit assembly dynamics defined by semi-quantitative mass spectrometry of purified complexes."
      Lebreton A., Rousselle J.C., Lenormand P., Namane A., Jacquier A., Fromont-Racine M., Saveanu C.
      Nucleic Acids Res. 36:4988-4999(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSOCIATION WITH THE PRE-60S PARTICLE.
    11. "Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunits."
      Ulbrich C., Diepholz M., Bassler J., Kressler D., Pertschy B., Galani K., Bottcher B., Hurt E.
      Cell 138:911-922(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ASSOCIATION WITH THE PRE-60S PARTICLE, INTERACTION WITH MDN1.
    12. "The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly."
      Bassler J., Kallas M., Pertschy B., Ulbrich C., Thoms M., Hurt E.
      Mol. Cell 38:712-721(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MDN1.

    Entry informationi

    Entry nameiNLE1_YEAST
    AccessioniPrimary (citable) accession number: P25382
    Secondary accession number(s): D6VR74, Q8NKJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: June 27, 2003
    Last modified: October 1, 2014
    This is version 121 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3