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Protein

Ribosome assembly protein 4

Gene

RSA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in ribosome biogenesis. Required for processing and efficient intra-nuclear transport of pre-60S ribosomal subunits. Interacts with the AAA-ATPase Midasin (MDN1/REA1), which is essential for the ATP-dependent dissociation of a group of nonribosomal factors from the pre-60S particle.3 Publications

GO - Biological processi

  • ribosomal large subunit assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-29372-MONOMER.
ReactomeiR-SCE-111458. Formation of apoptosome.
R-SCE-3214858. RMTs methylate histone arginines.
R-SCE-429958. mRNA decay by 3' to 5' exoribonuclease.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome assembly protein 41 Publication
Alternative name(s):
Notchless protein homolog 1By similarity
Ribosome biogenesis factor RSA41 Publication
Gene namesi
Name:RSA41 Publication
Ordered Locus Names:YCR072CImported
ORF Names:YCR72C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR072C.
SGDiS000000668. RSA4.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114E → A: Impairs interaction with MDN1. 1 Publication1
Mutagenesisi114E → D: Impairs interaction with MDN1. Blocks progression of the nascent pre-60S subunit and subsequent export to the cytoplasm. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000514711 – 515Ribosome assembly protein 4Add BLAST515

Proteomic databases

MaxQBiP25382.
PRIDEiP25382.

PTM databases

iPTMnetiP25382.

Interactioni

Subunit structurei

Associates with the pre-60S ribosomal particle. Interacts (via N-terminus) with MDN1/REA1 (via VWFA/MIDAS domain).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MDN1Q120196EBI-21980,EBI-10633
NSA2P400785EBI-21980,EBI-22681

Protein-protein interaction databases

BioGridi31047. 48 interactors.
DIPiDIP-5365N.
IntActiP25382. 19 interactors.
MINTiMINT-531710.

Structurei

Secondary structure

1515
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 41Combined sources8
Beta strandi48 – 50Combined sources3
Helixi57 – 68Combined sources12
Beta strandi77 – 80Combined sources4
Helixi103 – 105Combined sources3
Beta strandi117 – 126Combined sources10
Beta strandi146 – 151Combined sources6
Beta strandi156 – 163Combined sources8
Beta strandi168 – 172Combined sources5
Turni173 – 176Combined sources4
Beta strandi177 – 182Combined sources6
Beta strandi189 – 194Combined sources6
Beta strandi201 – 205Combined sources5
Beta strandi210 – 213Combined sources4
Turni215 – 217Combined sources3
Beta strandi232 – 237Combined sources6
Helixi240 – 242Combined sources3
Beta strandi251 – 255Combined sources5
Beta strandi260 – 264Combined sources5
Turni265 – 268Combined sources4
Beta strandi269 – 273Combined sources5
Beta strandi281 – 286Combined sources6
Beta strandi290 – 296Combined sources7
Beta strandi301 – 305Combined sources5
Helixi306 – 308Combined sources3
Beta strandi312 – 316Combined sources5
Beta strandi323 – 330Combined sources8
Helixi331 – 336Combined sources6
Helixi349 – 364Combined sources16
Beta strandi365 – 370Combined sources6
Beta strandi374 – 378Combined sources5
Beta strandi383 – 386Combined sources4
Turni388 – 390Combined sources3
Beta strandi395 – 398Combined sources4
Beta strandi405 – 410Combined sources6
Beta strandi414 – 421Combined sources8
Beta strandi426 – 430Combined sources5
Turni431 – 433Combined sources3
Beta strandi436 – 440Combined sources5
Beta strandi447 – 452Combined sources6
Beta strandi456 – 463Combined sources8
Beta strandi466 – 472Combined sources7
Turni473 – 476Combined sources4
Beta strandi477 – 483Combined sources7
Beta strandi489 – 494Combined sources6
Beta strandi500 – 505Combined sources6
Beta strandi510 – 514Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCTelectron microscopy3.08x1-515[»]
4V7Felectron microscopy8.70q1-515[»]
4WJUX-ray2.80A/B1-515[»]
4WJVX-ray3.20A/B/C/D137-515[»]
5FL8electron microscopy9.50q1-515[»]
ProteinModelPortaliP25382.
SMRiP25382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati141 – 181WD 1Sequence analysisAdd BLAST41
Repeati184 – 223WD 2Sequence analysisAdd BLAST40
Repeati227 – 273WD 3Sequence analysisAdd BLAST47
Repeati276 – 314WD 4Sequence analysisAdd BLAST39
Repeati352 – 396WD 5Sequence analysisAdd BLAST45
Repeati400 – 439WD 6Sequence analysisAdd BLAST40
Repeati442 – 481WD 7Sequence analysisAdd BLAST40
Repeati484 – 515WD 8Sequence analysisAdd BLAST32

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 128Interaction with MDN11 PublicationAdd BLAST109

Sequence similaritiesi

Belongs to the NLE1/RSA4 family.Curated
Contains 8 WD repeats.Sequence analysis

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00860000133793.
InParanoidiP25382.
KOiK14855.
OMAiVTSLAWE.
OrthoDBiEOG092C10HM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR012972. NLE.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08154. NLE. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 5 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25382-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLIPPPSK KQKKEAQLPR EVAIIPKDLP NVSIKFQALD TGDNVGGALR
60 70 80 90 100
VPGAISEKQL EELLNQLNGT SDDPVPYTFS CTIQGKKASD PVKTIDITDN
110 120 130 140 150
LYSSLIKPGY NSTEDQITLL YTPRAVFKVK PVTRSSSAIA GHGSTILCSA
160 170 180 190 200
FAPHTSSRMV TGAGDNTARI WDCDTQTPMH TLKGHYNWVL CVSWSPDGEV
210 220 230 240 250
IATGSMDNTI RLWDPKSGQC LGDALRGHSK WITSLSWEPI HLVKPGSKPR
260 270 280 290 300
LASSSKDGTI KIWDTVSRVC QYTMSGHTNS VSCVKWGGQG LLYSGSHDRT
310 320 330 340 350
VRVWDINSQG RCINILKSHA HWVNHLSLST DYALRIGAFD HTGKKPSTPE
360 370 380 390 400
EAQKKALENY EKICKKNGNS EEMMVTASDD YTMFLWNPLK STKPIARMTG
410 420 430 440 450
HQKLVNHVAF SPDGRYIVSA SFDNSIKLWD GRDGKFISTF RGHVASVYQV
460 470 480 490 500
AWSSDCRLLV SCSKDTTLKV WDVRTRKLSV DLPGHKDEVY TVDWSVDGKR
510
VCSGGKDKMV RLWTH
Length:515
Mass (Da):57,026
Last modified:June 27, 2003 - v3
Checksum:i04451B0C38FB5318
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42989.1.
AY692666 Genomic DNA. Translation: AAT92685.1.
BK006937 Genomic DNA. Translation: DAA07543.1.
PIRiS19487.
RefSeqiNP_009997.2. NM_001178782.1.

Genome annotation databases

EnsemblFungiiCAC42989; CAC42989; CAC42989.
YCR072C; YCR072C; YCR072C.
GeneIDi850435.
KEGGisce:YCR072C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42989.1.
AY692666 Genomic DNA. Translation: AAT92685.1.
BK006937 Genomic DNA. Translation: DAA07543.1.
PIRiS19487.
RefSeqiNP_009997.2. NM_001178782.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCTelectron microscopy3.08x1-515[»]
4V7Felectron microscopy8.70q1-515[»]
4WJUX-ray2.80A/B1-515[»]
4WJVX-ray3.20A/B/C/D137-515[»]
5FL8electron microscopy9.50q1-515[»]
ProteinModelPortaliP25382.
SMRiP25382.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31047. 48 interactors.
DIPiDIP-5365N.
IntActiP25382. 19 interactors.
MINTiMINT-531710.

PTM databases

iPTMnetiP25382.

Proteomic databases

MaxQBiP25382.
PRIDEiP25382.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAC42989; CAC42989; CAC42989.
YCR072C; YCR072C; YCR072C.
GeneIDi850435.
KEGGisce:YCR072C.

Organism-specific databases

EuPathDBiFungiDB:YCR072C.
SGDiS000000668. RSA4.

Phylogenomic databases

GeneTreeiENSGT00860000133793.
InParanoidiP25382.
KOiK14855.
OMAiVTSLAWE.
OrthoDBiEOG092C10HM.

Enzyme and pathway databases

BioCyciYEAST:G3O-29372-MONOMER.
ReactomeiR-SCE-111458. Formation of apoptosome.
R-SCE-3214858. RMTs methylate histone arginines.
R-SCE-429958. mRNA decay by 3' to 5' exoribonuclease.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-72165. mRNA Splicing - Minor Pathway.

Miscellaneous databases

PROiP25382.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR012972. NLE.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08154. NLE. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 5 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNLE1_YEAST
AccessioniPrimary (citable) accession number: P25382
Secondary accession number(s): D6VR74, Q8NKJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 27, 2003
Last modified: November 30, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.