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Protein

Subtilase-type proteinase RRT12

Gene

RRT12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subtilisin-related protease involved in the formation of a protective dityrosine layer required for spore wall assembly. Identified in a screen for mutants with increased levels of rDNA transcription.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei174Charge relay systemBy similarity1
Active sitei205Charge relay systemBy similarity1
Active sitei365Charge relay systemBy similarity1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: SGD

GO - Biological processi

  • ascospore wall assembly Source: SGD

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processSporulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29356-MONOMER
ReactomeiR-SCE-8866427 VLDLR internalisation and degradation
R-SCE-8964038 LDL clearance

Protein family/group databases

MEROPSiS08.A50

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilase-type proteinase RRT12 (EC:3.4.21.-)
Alternative name(s):
Outer spore wall protein 3
Regulator of rDNA transcription protein 12
Gene namesi
Name:RRT12
Synonyms:OSW3
Ordered Locus Names:YCR045C
ORF Names:YCR45C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR045C
SGDiS000000641 RRT12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi365S → A: Abolishes protease activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000002720518 – 491Subtilase-type proteinase RRT12Add BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi64N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi106N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi121N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi268N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi356N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi449N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP25381
PRIDEiP25381

Interactioni

Protein-protein interaction databases

BioGridi31027, 49 interactors
DIPiDIP-1543N
IntActiP25381, 1 interactor
MINTiP25381
STRINGi4932.YCR045C

Structurei

3D structure databases

ProteinModelPortaliP25381
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 389Peptidase S8Add BLAST234

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00490000043472
InParanoidiP25381
KOiK14644
OMAiITEDIMF
OrthoDBiEOG092C4A4B

Family and domain databases

CDDicd04077 Peptidases_S8_PCSK9_Proteinase, 1 hit
Gene3Di3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR034193 PCSK9_ProteinaseK-like
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
PfamiView protein in Pfam
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25381-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPQCILISL LVNLAYAEEY LVRFKNPTAF QQFTSNSNRS WRQFIDNKIE
60 70 80 90 100
KKFSIGSFRG VTMNLSKNLV NKLKKSPLVA DIVPNFRFEA FEGDSVNSAE
110 120 130 140 150
SSYTFNATAK YSYEDVEEEQ NITYQPDAPR HLARISRHYQ LPFDVGDKDR
160 170 180 190 200
YKSWFNYYYE HDYQGQDVNA YIMDTGIFAD HPEFEDRVIQ GIDLTKEGFG
210 220 230 240 250
DQNGHGTHVA GLVGSKTYGA AKRVNLVEVK VLGKDGSGEA SNVLSGLEFI
260 270 280 290 300
VEHCTKVSRP QGKKCVANLS LGSFRSPIIN MAVEGAIEEG IVFVAAAGNF
310 320 330 340 350
NLDAYWASPA SAENVITVGA FDDHIDTIAK FSNWGPCVNI FAPGVEIESL
360 370 380 390 400
SHLNYNDTLI LSGTSMSTPI VTGVAAILLS KGIEPEMIAQ EIEYLSTRNV
410 420 430 440 450
FHRRTLFFKP STPNQILYNG VDKLDDPYDD ETFPRLNIEA IAKELEEYNA
460 470 480 490
TLQTPMSENL QSGSKLWGWN NDVTLPLGEI RLKRRDFMKN L
Length:491
Mass (Da):55,068
Last modified:May 1, 1992 - v1
Checksum:i7C8000FF84CDDF5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA Translation: CAA42293.1
BK006937 Genomic DNA Translation: DAA07523.1
PIRiS19458
RefSeqiNP_009974.1, NM_001178759.1

Genome annotation databases

EnsemblFungiiCAA42293; CAA42293; CAA42293
YCR045C; YCR045C; YCR045C
GeneIDi850412
KEGGisce:YCR045C

Similar proteinsi

Entry informationi

Entry nameiRRT12_YEAST
AccessioniPrimary (citable) accession number: P25381
Secondary accession number(s): D6VR54
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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