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Protein

General amino acid permease AGP1

Gene

AGP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Broad substrate range permease which transports asparagine and glutamine with intermediate specificity. Also transports Ala, Cys, Gly, Ile, Leu, Met, Phe, Ser, Thr, Tyr and Val. Important for the utilization of amino acids as a nitrogen source.2 Publications

Miscellaneous

Present with 21100 molecules/cell in log phase SD medium.1 Publication

Kineticsi

  1. KM=0.23 mM for leucine1 Publication
  1. Vmax=2.6 nmol/min/mg enzyme for leucine transport1 Publication

GO - Molecular functioni

  • amino acid transmembrane transporter activity Source: SGD
  • L-phenylalanine transmembrane transporter activity Source: CACAO
  • L-proline transmembrane transporter activity Source: SGD

GO - Biological processi

  • amino acid transport Source: SGD
  • transmembrane transport Source: SGD

Keywordsi

Biological processAmino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29287-MONOMER
SABIO-RKiP25376

Protein family/group databases

TCDBi2.A.3.10.7 the amino acid-polyamine-organocation (apc) family

Names & Taxonomyi

Protein namesi
Recommended name:
General amino acid permease AGP1
Alternative name(s):
Asparagine/glutamine permease
Gene namesi
Name:AGP1
Ordered Locus Names:YCL025C
ORF Names:YCL25C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL025C
SGDiS000000530 AGP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 124CytoplasmicSequence analysisAdd BLAST124
Transmembranei125 – 145HelicalSequence analysisAdd BLAST21
Topological domaini146 – 148ExtracellularSequence analysis3
Transmembranei149 – 169HelicalSequence analysisAdd BLAST21
Topological domaini170 – 197CytoplasmicSequence analysisAdd BLAST28
Transmembranei198 – 218HelicalSequence analysisAdd BLAST21
Topological domaini219 – 231ExtracellularSequence analysisAdd BLAST13
Transmembranei232 – 252HelicalSequence analysisAdd BLAST21
Topological domaini253 – 260CytoplasmicSequence analysis8
Transmembranei261 – 281HelicalSequence analysisAdd BLAST21
Topological domaini282 – 313ExtracellularSequence analysisAdd BLAST32
Transmembranei314 – 334HelicalSequence analysisAdd BLAST21
Topological domaini335 – 352CytoplasmicSequence analysisAdd BLAST18
Transmembranei353 – 373HelicalSequence analysisAdd BLAST21
Topological domaini374 – 402ExtracellularSequence analysisAdd BLAST29
Transmembranei403 – 425HelicalSequence analysisAdd BLAST23
Topological domaini426 – 452CytoplasmicSequence analysisAdd BLAST27
Transmembranei453 – 473HelicalSequence analysisAdd BLAST21
Topological domaini474 – 477ExtracellularSequence analysis4
Transmembranei478 – 498HelicalSequence analysisAdd BLAST21
Topological domaini499 – 531CytoplasmicSequence analysisAdd BLAST33
Transmembranei532 – 552HelicalSequence analysisAdd BLAST21
Topological domaini553 – 560ExtracellularSequence analysis8
Transmembranei561 – 581HelicalSequence analysisAdd BLAST21
Topological domaini582 – 633CytoplasmicSequence analysisAdd BLAST52

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1741178

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541421 – 633General amino acid permease AGP1Add BLAST633

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineBy similarity1
Cross-linki11Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Lipidationi633S-palmitoyl cysteineBy similarity1

Post-translational modificationi

Palmitoylated by PFA4.1 Publication

Keywords - PTMi

Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP25376
PaxDbiP25376
PRIDEiP25376

PTM databases

iPTMnetiP25376
SwissPalmiP25376

Expressioni

Inductioni

Induced by transcription factors DAL81 and STP1, which are activated by a signal initiated by the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor system in response to external amino acid levels.3 Publications

Interactioni

Protein-protein interaction databases

BioGridi30959, 274 interactors
DIPiDIP-4973N
IntActiP25376, 37 interactors
MINTiP25376
STRINGi4932.YCL025C

Chemistry databases

BindingDBiP25376

Structurei

3D structure databases

ProteinModelPortaliP25376
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000049744
HOGENOMiHOG000261848
InParanoidiP25376
KOiK16261
OMAiHVMNFIL
OrthoDBiEOG092C164R

Family and domain databases

InterProiView protein in InterPro
IPR004841 AA-permease/SLC12A_dom
IPR002293 AA/rel_permease1
IPR004762 Amino_acid_permease_fungi
IPR004840 Amoino_acid_permease_CS
PfamiView protein in Pfam
PF00324 AA_permease, 1 hit
PIRSFiPIRSF006060 AA_transporter, 1 hit
TIGRFAMsiTIGR00913 2A0310, 1 hit
PROSITEiView protein in PROSITE
PS00218 AMINO_ACID_PERMEASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

P25376-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSKSLYEL KDLKNSSTEI HATGQDNEIE YFETGSNDRP SSQPHLGYEQ
60 70 80 90 100
HNTSAVRRFF DSFKRADQGP QDEVEATQMN DLTSAISPSS RQAQELEKNE
110 120 130 140 150
SSDNIGANTG HKSDSLKKTI QPRHVLMIAL GTGIGTGLLV GNGTALVHAG
160 170 180 190 200
PAGLLIGYAI MGSILYCIIQ ACGEMALVYS NLTGGYNAYP SFLVDDGFGF
210 220 230 240 250
AVAWVYCLQW LCVCPLELVT ASMTIKYWTT SVNPDVFVII FYVLVITINI
260 270 280 290 300
FGARGYAEAE FFFNCCKILM MTGFFILGII IDVGGAGNDG FIGGKYWHDP
310 320 330 340 350
GAFNGKHAID RFKGVAATLV TAAFAFGGSE FIAITTAEQS NPRKAIPGAA
360 370 380 390 400
KQMIYRILFL FLATIILLGF LVPYNSDQLL GSTGGGTKAS PYVIAVASHG
410 420 430 440 450
VRVVPHFINA VILLSVLSMA NSSFYSSARL FLTLSEQGYA PKVFSYIDRA
460 470 480 490 500
GRPLIAMGVS ALFAVIAFCA ASPKEEQVFT WLLAISGLSQ LFTWTAICLS
510 520 530 540 550
HLRFRRAMKV QGRSLGELGF KSQTGVWGSA YACIMMILIL IAQFWVAIAP
560 570 580 590 600
IGEGKLDAQA FFENYLAMPI LIALYVGYKV WHKDWKLFIR ADKIDLDSHR
610 620 630
QIFDEELIKQ EDEEYRERLR NGPYWKRVVA FWC
Length:633
Mass (Da):69,671
Last modified:April 29, 2008 - v3
Checksum:i8E19A836C2F6C50F
GO

Sequence cautioni

The sequence CAA42360 differs from that shown. Reason: Frameshift at position 591.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA Translation: CAA42360.2 Frameshift.
BK006937 Genomic DNA Translation: DAA07460.1
PIRiS19352
RefSeqiNP_009905.3, NM_001178671.1

Genome annotation databases

EnsemblFungiiCAA42360; CAA42360; CAA42360
YCL025C; YCL025C; YCL025C
GeneIDi850333
KEGGisce:YCL025C

Similar proteinsi

Entry informationi

Entry nameiAGP1_YEAST
AccessioniPrimary (citable) accession number: P25376
Secondary accession number(s): D6VQZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: April 29, 2008
Last modified: May 23, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

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