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Protein

Saccharolysin

Gene

PRD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could be involved in late stage of protein degradation.

Miscellaneous

Present with 8910 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Cleavage of Pro-|-Phe and Ala-|-Ala bonds.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi501Zinc; catalyticPROSITE-ProRule annotation1
Active sitei502PROSITE-ProRule annotation1
Metal bindingi505Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi508Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: SGD

GO - Biological processi

  • peptide metabolic process Source: GO_Central
  • proteolysis Source: SGD

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YCL057W-MONOMER.
ReactomeiR-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiM03.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Saccharolysin (EC:3.4.24.37)
Alternative name(s):
Oligopeptidase YSCD
Protease D
Proteinase yscD
Gene namesi
Name:PRD1
Ordered Locus Names:YCL057W
ORF Names:YCL57W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL057W.
SGDiS000000562. PRD1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000781561 – 712SaccharolysinAdd BLAST712

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25375.
PRIDEiP25375.

PTM databases

iPTMnetiP25375.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CCNB1IP1Q9NPC33EBI-13932,EBI-745269From Homo sapiens.

Protein-protein interaction databases

BioGridi30929. 16 interactors.
DIPiDIP-4938N.
IntActiP25375. 8 interactors.
MINTiMINT-542010.
STRINGi4932.YCL057W.

Structurei

3D structure databases

ProteinModelPortaliP25375.
SMRiP25375.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074738.
HOGENOMiHOG000245985.
InParanoidiP25375.
KOiK01405.
OMAiINGVAWD.
OrthoDBiEOG092C11XF.

Family and domain databases

Gene3Di1.20.1050.40. 1 hit.
InterProiView protein in InterPro
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
PfamiView protein in Pfam
PF01432. Peptidase_M3. 1 hit.
PROSITEiView protein in PROSITE
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P25375-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLLCKNWF ASPVISPLLY TRSLYSMANT TSFPIAPQAP PNWSFTPSDI
60 70 80 90 100
SGKTNEIINN SNNFYDSMSK VESPSVSNFV EPFMKFENEL GPIINQLTFL
110 120 130 140 150
QHVSSDKEIR DASVNSSMKL DELNIDLSLR HDIFLQFARV WQDVQSKADS
160 170 180 190 200
VERETFKYVE KSYKDYIHSG LELDEGNRLK IKEIKKKISV NSINFSKNLG
210 220 230 240 250
EQKEYITFTK EQLEGVPDSI LTQFETIKSD KDSNETLYKV TFKYPDIFPV
260 270 280 290 300
MKLASSAQTR KQAFLADQNK VPENEAILLD TLKLRDELAS LLGYDTYANY
310 320 330 340 350
NLYDKMAEDS TTVMNFLNDL KDKLIPLGRK ELQVLQDMKA EDVKKLNQGA
360 370 380 390 400
DPNYYIWDHR YYDNKYLLEN FNVDLEKISE YFPLEATITG MLEIYETLFN
410 420 430 440 450
LKFIETKDSQ NKSVWHDDVK QIAVWNMDDP KSPNFVGWIY FDLHPRDGKY
460 470 480 490 500
GHAANFGLSS SFMIDDTTRS YPVTALVCNF SKSTKDKPSL LKHNEIVTFF
510 520 530 540 550
HELGHGIHDL VGQNKESRFN GPGSVPWDFV EAPSQMLEFW TWNKNELINL
560 570 580 590 600
SSHYKTGEKI PESLINSLIK TKHVNGALFT LRQLHFGLFD MKVHTCKDLQ
610 620 630 640 650
NLSICDTWNQ LRQDISLISN GGTLSKGYDS FGHIMSDSYS AGYYGYLWAE
660 670 680 690 700
VFATDMYHTK FAKDPLNAKN GIQYRDIVLA RGGLYDINDN LKEFLGREPS
710
KDAFLKELGL QN
Length:712
Mass (Da):81,934
Last modified:May 1, 1992 - v1
Checksum:i340910B7FDAFBE37
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti384L → P in AAU09678 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76504 Genomic DNA. Translation: CAA54039.1.
X59720 Genomic DNA. Translation: CAA42388.1.
AY723761 Genomic DNA. Translation: AAU09678.1.
BK006937 Genomic DNA. Translation: DAA07429.1.
PIRiS19387.
RefSeqiNP_009874.1. NM_001178701.1.

Genome annotation databases

EnsemblFungiiCAA42388; CAA42388; CAA42388.
YCL057W; YCL057W; YCL057W.
GeneIDi850301.
KEGGisce:YCL057W.

Similar proteinsi

Entry informationi

Entry nameiPRTD_YEAST
AccessioniPrimary (citable) accession number: P25375
Secondary accession number(s): D6VQW0, E9P942
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 27, 2017
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names