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P25374

- NFS1_YEAST

UniProt

P25374 - NFS1_YEAST

Protein

Cysteine desulfurase, mitochondrial

Gene

NFS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (15 Mar 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. Involved in both tRNA-processing and mitochondrial metabolism.

    Catalytic activityi

    L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.

    Cofactori

    Pyridoxal phosphate.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei421 – 4211By similarity

    GO - Molecular functioni

    1. cysteine desulfurase activity Source: SGD
    2. protein binding Source: IntAct
    3. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. cellular iron ion homeostasis Source: SGD
    2. cysteine metabolic process Source: InterPro
    3. iron-sulfur cluster assembly Source: SGD
    4. mitochondrial tRNA thio-modification Source: SGD
    5. tRNA thio-modification Source: SGD
    6. tRNA wobble uridine modification Source: SGD

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-29283-MONOMER.
    YEAST:G3O-29283-MONOMER.
    ReactomeiREACT_189017. Mitochondrial iron-sulfur cluster biogenesis.
    REACT_189051. Molybdenum cofactor biosynthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine desulfurase, mitochondrial (EC:2.8.1.7)
    Alternative name(s):
    tRNA-splicing protein SPL1
    Gene namesi
    Name:NFS1
    Synonyms:SPL1
    Ordered Locus Names:YCL017C
    ORF Names:YCL17C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCL017c.
    SGDiS000000522. NFS1.

    Subcellular locationi

    GO - Cellular componenti

    1. L-cysteine desulfurase complex Source: SGD
    2. mitochondrion Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333MitochondrionSequence AnalysisAdd
    BLAST
    Chaini34 – 497464Cysteine desulfurase, mitochondrialPRO_0000001304Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei299 – 2991N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    MaxQBiP25374.
    PaxDbiP25374.
    PeptideAtlasiP25374.
    PRIDEiP25374.

    Expressioni

    Gene expression databases

    GenevestigatoriP25374.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ISD11Q6Q56010EBI-11991,EBI-784315
    ISU1Q030202EBI-11991,EBI-29901

    Protein-protein interaction databases

    BioGridi30967. 20 interactions.
    DIPiDIP-2948N.
    IntActiP25374. 14 interactions.
    MINTiMINT-474525.
    STRINGi4932.YCL017C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25374.
    SMRiP25374. Positions 99-491.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1104.
    GeneTreeiENSGT00530000063513.
    HOGENOMiHOG000017510.
    KOiK04487.
    OMAiVDYTVQK.
    OrthoDBiEOG7ZKSMC.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR000192. Aminotrans_V/Cys_dSase.
    IPR020578. Aminotrans_V_PyrdxlP_BS.
    IPR010240. Cys_deSase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00266. Aminotran_5. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR02006. IscS. 1 hit.
    PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25374-1 [UniParc]FASTAAdd to Basket

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    MLKSTATRSI TRLSQVYNVP AATYRACLVS RRFYSPPAAG VKLDDNFSLE    50
    THTDIQAAAK AQASARASAS GTTPDAVVAS GSTAMSHAYQ ENTGFGTRPI 100
    YLDMQATTPT DPRVLDTMLK FYTGLYGNPH SNTHSYGWET NTAVENARAH 150
    VAKMINADPK EIIFTSGATE SNNMVLKGVP RFYKKTKKHI ITTRTEHKCV 200
    LEAARAMMKE GFEVTFLNVD DQGLIDLKEL EDAIRPDTCL VSVMAVNNEI 250
    GVIQPIKEIG AICRKNKIYF HTDAAQAYGK IHIDVNEMNI DLLSISSHKI 300
    YGPKGIGAIY VRRRPRVRLE PLLSGGGQER GLRSGTLAPP LVAGFGEAAR 350
    LMKKEFDNDQ AHIKRLSDKL VKGLLSAEHT TLNGSPDHRY PGCVNVSFAY 400
    VEGESLLMAL RDIALSSGSA CTSASLEPSY VLHALGKDDA LAHSSIRFGI 450
    GRFSTEEEVD YVVKAVSDRV KFLRELSPLW EMVQEGIDLN SIKWSGH 497
    Length:497
    Mass (Da):54,467
    Last modified:March 15, 2004 - v2
    Checksum:iD709E326B3921BCC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti150 – 1501H → Y in AAA34814. (PubMed:8444805)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98808 Genomic DNA. Translation: AAA34814.1.
    X59720 Genomic DNA. Translation: CAA42344.2.
    M12909 Genomic DNA. Translation: AAA66918.1.
    BK006937 Genomic DNA. Translation: DAA07466.1.
    PIRiS19343.
    RefSeqiNP_009912.2. NM_001178664.1.

    Genome annotation databases

    EnsemblFungiiYCL017C; YCL017C; YCL017C.
    GeneIDi850343.
    KEGGisce:YCL017C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98808 Genomic DNA. Translation: AAA34814.1 .
    X59720 Genomic DNA. Translation: CAA42344.2 .
    M12909 Genomic DNA. Translation: AAA66918.1 .
    BK006937 Genomic DNA. Translation: DAA07466.1 .
    PIRi S19343.
    RefSeqi NP_009912.2. NM_001178664.1.

    3D structure databases

    ProteinModelPortali P25374.
    SMRi P25374. Positions 99-491.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30967. 20 interactions.
    DIPi DIP-2948N.
    IntActi P25374. 14 interactions.
    MINTi MINT-474525.
    STRINGi 4932.YCL017C.

    Proteomic databases

    MaxQBi P25374.
    PaxDbi P25374.
    PeptideAtlasi P25374.
    PRIDEi P25374.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCL017C ; YCL017C ; YCL017C .
    GeneIDi 850343.
    KEGGi sce:YCL017C.

    Organism-specific databases

    CYGDi YCL017c.
    SGDi S000000522. NFS1.

    Phylogenomic databases

    eggNOGi COG1104.
    GeneTreei ENSGT00530000063513.
    HOGENOMi HOG000017510.
    KOi K04487.
    OMAi VDYTVQK.
    OrthoDBi EOG7ZKSMC.

    Enzyme and pathway databases

    BioCyci MetaCyc:G3O-29283-MONOMER.
    YEAST:G3O-29283-MONOMER.
    Reactomei REACT_189017. Mitochondrial iron-sulfur cluster biogenesis.
    REACT_189051. Molybdenum cofactor biosynthesis.

    Miscellaneous databases

    NextBioi 965791.
    PROi P25374.

    Gene expression databases

    Genevestigatori P25374.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR000192. Aminotrans_V/Cys_dSase.
    IPR020578. Aminotrans_V_PyrdxlP_BS.
    IPR010240. Cys_deSase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00266. Aminotran_5. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR02006. IscS. 1 hit.
    PROSITEi PS00595. AA_TRANSFER_CLASS_5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "SPL1-1, a Saccharomyces cerevisiae mutation affecting tRNA splicing."
      Leong-Morgenthaler P.M., Kolman C., Oliver S.G., Hottinger H., Soell D.
      J. Bacteriol. 175:1433-1442(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Valles G., Volckaerts G.
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 150.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Nucleotide sequence of the 3' terminal region of the LEU2 gene from Saccharomyces cerevisiae."
      Froman B.E., Tait R.C., Rodriguez R.L.
      Gene 31:257-261(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 459-497.
    6. "The mitochondrial proteins Atm1p and Nfs1p are essential for biogenesis of cytosolic Fe/S proteins."
      Kispal G., Csere P., Prohl C., Lill R.
      EMBO J. 18:3981-3989(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. "Yeast mitochondrial protein, Nfs1p, coordinately regulates iron-sulfur cluster proteins, cellular iron uptake, and iron distribution."
      Li J., Kogan M., Knight S.A., Pain D., Dancis A.
      J. Biol. Chem. 274:33025-33034(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNFS1_YEAST
    AccessioniPrimary (citable) accession number: P25374
    Secondary accession number(s): D6VQZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: March 15, 2004
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 504 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3