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Protein

Glutaredoxin-1

Gene

GRX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the glutathione system which performs several activities such as glutathione-dependent oxidoreductase, glutathione peroxidase and glutathione S-transferase (GST) activity. The disulfide bond functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. In addition, it is also involved in reducing cytosolic protein- and non-protein-disulfides in a coupled system with glutathione reductase. Required for resistance to reactive oxygen species (ROS) by directly reducing hydroperoxides and for the detoxification of ROS-mediated damage. GRX1 is less active as an oxidoreductase than GRX2.5 Publications

Kineticsi

  1. KM=0.88 mM for H2O22 Publications
  2. KM=0.52 mM for cumene hydroperoxide2 Publications
  3. KM=0.37 mM for tert-butyl hydroperoxide2 Publications
  4. KM=4.2 mM for 1-chloro-2,4-dinitrobenzene2 Publications
  5. KM=1.3 mM for 1,2-dichloro-4-nitrobenzene2 Publications
  6. KM=6.2 mM for reduced glutathione1 Publication
  1. Vmax=110 µmol/min/mg enzyme for H2O22 Publications
  2. Vmax=32.5 µmol/min/mg enzyme for cumene hydroperoxide2 Publications
  3. Vmax=7.5 µmol/min/mg enzyme for tert-butyl hydroperoxide2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631Glutathione2 Publications
Binding sitei75 – 751Glutathione; via amide nitrogen and carbonyl oxygen2 Publications

GO - Molecular functioni

  • disulfide oxidoreductase activity Source: SGD
  • electron carrier activity Source: InterPro
  • glutathione peroxidase activity Source: SGD
  • glutathione transferase activity Source: SGD
  • protein disulfide oxidoreductase activity Source: InterPro

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • cellular response to oxidative stress Source: SGD
  • protein glutathionylation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Enzyme and pathway databases

ReactomeiR-SCE-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
SABIO-RKP25373.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaredoxin-1
Alternative name(s):
Glutathione-dependent oxidoreductase 1
Gene namesi
Name:GRX1
Ordered Locus Names:YCL035C
ORF Names:YCL35C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL035C.
SGDiS000000540. GRX1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi30 – 301C → S: Leads to increased oxidoreductase activity. 1 Publication
Mutagenesisi89 – 891D → S: Leads to increased oxidoreductase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 110110Glutaredoxin-1PRO_0000141613Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki11 – 11Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Disulfide bondi27 ↔ 30Redox-active; alternate1 Publication
Modified residuei27 – 271S-glutathionyl cysteine; alternate2 Publications

Keywords - PTMi

Disulfide bond, Glutathionylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP25373.

Expressioni

Inductioni

In response to heat shock and osmotic stress.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi30948. 37 interactions.
DIPiDIP-5646N.
IntActiP25373. 2 interactions.
MINTiMINT-556392.

Structurei

Secondary structure

1
110
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1613Combined sources
Beta strandi17 – 237Combined sources
Helixi28 – 3811Combined sources
Turni39 – 413Combined sources
Helixi45 – 473Combined sources
Beta strandi48 – 525Combined sources
Helixi53 – 553Combined sources
Helixi59 – 7012Combined sources
Beta strandi77 – 804Combined sources
Beta strandi83 – 875Combined sources
Helixi88 – 969Combined sources
Helixi99 – 10810Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JACX-ray2.02A1-110[»]
2JADX-ray2.70A1-110[»]
3C1RX-ray2.00A1-110[»]
3C1SX-ray2.50A1-110[»]
ProteinModelPortaliP25373.
SMRiP25373. Positions 1-92.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25373.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 110104GlutaredoxinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 296Glutathione binding2 Publications
Regioni88 – 892Glutathione binding2 Publications

Sequence similaritiesi

Belongs to the glutaredoxin family.Curated
Contains 1 glutaredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

GeneTreeiENSGT00390000003677.
HOGENOMiHOG000095204.
InParanoidiP25373.
KOiK03676.
OMAiITKDAYI.
OrthoDBiEOG092C3ZJC.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR011767. GLR_AS.
IPR002109. Glutaredoxin.
IPR011899. Glutaredoxin_euk/vir.
IPR014025. Glutaredoxin_subgr.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
PRINTSiPR00160. GLUTAREDOXIN.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR02180. GRX_euk. 1 hit.
PROSITEiPS00195. GLUTAREDOXIN_1. 1 hit.
PS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25373-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSQETIKHV KDLIAENEIF VASKTYCPYC HAALNTLFEK LKVPRSKVLV
60 70 80 90 100
LQLNDMKEGA DIQAALYEIN GQRTVPNIYI NGKHIGGNDD LQELRETGEL
110
EELLEPILAN
Length:110
Mass (Da):12,380
Last modified:May 1, 1992 - v1
Checksum:i8E69BC80BF40F2DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42381.1.
BK006937 Genomic DNA. Translation: DAA07449.1.
PIRiS19363.
RefSeqiNP_009895.1. NM_001178680.1.

Genome annotation databases

EnsemblFungiiCAA42381; CAA42381; CAA42381.
YCL035C; YCL035C; YCL035C.
GeneIDi850322.
KEGGisce:YCL035C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42381.1.
BK006937 Genomic DNA. Translation: DAA07449.1.
PIRiS19363.
RefSeqiNP_009895.1. NM_001178680.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JACX-ray2.02A1-110[»]
2JADX-ray2.70A1-110[»]
3C1RX-ray2.00A1-110[»]
3C1SX-ray2.50A1-110[»]
ProteinModelPortaliP25373.
SMRiP25373. Positions 1-92.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30948. 37 interactions.
DIPiDIP-5646N.
IntActiP25373. 2 interactions.
MINTiMINT-556392.

Proteomic databases

MaxQBiP25373.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42381; CAA42381; CAA42381.
YCL035C; YCL035C; YCL035C.
GeneIDi850322.
KEGGisce:YCL035C.

Organism-specific databases

EuPathDBiFungiDB:YCL035C.
SGDiS000000540. GRX1.

Phylogenomic databases

GeneTreeiENSGT00390000003677.
HOGENOMiHOG000095204.
InParanoidiP25373.
KOiK03676.
OMAiITKDAYI.
OrthoDBiEOG092C3ZJC.

Enzyme and pathway databases

ReactomeiR-SCE-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
SABIO-RKP25373.

Miscellaneous databases

EvolutionaryTraceiP25373.
PROiP25373.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR011767. GLR_AS.
IPR002109. Glutaredoxin.
IPR011899. Glutaredoxin_euk/vir.
IPR014025. Glutaredoxin_subgr.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
PRINTSiPR00160. GLUTAREDOXIN.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR02180. GRX_euk. 1 hit.
PROSITEiPS00195. GLUTAREDOXIN_1. 1 hit.
PS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLRX1_YEAST
AccessioniPrimary (citable) accession number: P25373
Secondary accession number(s): D6VQY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 7, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3030 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.