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P25369 (LSB5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
LAS seventeen-binding protein 5
Short name=LAS17-binding protein 5
Gene names
Name:LSB5
Ordered Locus Names:YCL034W
ORF Names:YCL186, YCL34W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for the organization of the actin cytoskeleton, fluid phase endocytosis and vesicle trafficking, together with YSC84. Ref.6

Subunit structure

Interacts with SLA1 and LAS17. Ref.6 Ref.7

Subcellular location

Cytoplasm › cell cortex. Cytoplasm › cytoskeleton. Note: Localizes independently of F-actin. Ref.6

Sequence similarities

Belongs to the LSB5 family.

Contains 1 VHS domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ARF3P409942EBI-10218,EBI-2824
LAS17Q124464EBI-10218,EBI-10022
SLA1P327905EBI-10218,EBI-17313

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354LAS seventeen-binding protein 5
PRO_0000084502

Regions

Domain15 – 161147VHS

Amino acid modifications

Modified residue1871Phosphothreonine Ref.8
Modified residue1931Phosphoserine Ref.8
Modified residue1951Phosphothreonine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P25369 [UniParc].

Last modified June 27, 2003. Version 3.
Checksum: 35772B575D40BEB2

FASTA35439,872
        10         20         30         40         50         60 
MGFLSDHPHT AITETIFRIV SSRDYTLEVE LAPLIQLIKA DHNDYNYTVN QEEAARALRK 

        70         80         90        100        110        120 
KIKYGNRLQQ SRTLDLLDLF ISQGVKFTVM YNDDKLLQRL RGMATNSENS GSGEKYEPRI 

       130        140        150        160        170        180 
IKKCAAYAIS WLNYITQNNL ENARAYSGLY QLGQTVKQRY SKSSRSRRSG GGSGGRSNFM 

       190        200        210        220        230        240 
DDSADDTLYQ SNSLTSADRL YRIPQINMNK EAPRIRLIIS DALASAVSLQ NSLIGLPKGK 

       250        260        270        280        290        300 
FSTDDEEATS KFIQARAIRR KVLRYLQLVT EGEFLGSLIH ANDELVAALT AYDDRSAQDD 

       310        320        330        340        350 
SSDESDHGSY DDGIYDENEQ DNSRYIDSES SEEESLSSYQ PSTISNPFGD HNKI

« Hide

References

« Hide 'large scale' references
[1]"The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames."
Rad M.R., Luetzenkirchen K., Xu G., Kleinhans U., Hollenberg C.P.
Yeast 7:533-538(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Gromadka R.
Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[4]Valles G., Volckaerts G.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Novel proteins linking the actin cytoskeleton to the endocytic machinery in Saccharomyces cerevisiae."
Dewar H., Warren D.T., Gardiner F.C., Gourlay C.G., Satish N., Richardson M.R., Andrews P.D., Ayscough K.R.
Mol. Biol. Cell 13:3646-3661(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE, FUNCTION, INTERACTION WITH SLA1, SUBCELLULAR LOCATION.
[7]"The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex."
Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B.
Mol. Biol. Cell 10:3521-3538(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LAS17.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-187; SER-193 AND THR-195, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X59720 Genomic DNA. Translation: CAC42957.1.
BK006937 Genomic DNA. Translation: DAA07450.1.
PIRS74282.
RefSeqNP_009896.2. NM_001178679.1.

3D structure databases

ProteinModelPortalP25369.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-6258N.
IntActP25369. 12 interactions.
MINTMINT-375039.

Proteomic databases

PaxDbP25369.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCL034W; YCL034W; YCL034W.
GeneID850323.
KEGGsce:YCL034W.

Organism-specific databases

CYGDYCL034w.
SGDS000000539. LSB5.

Phylogenomic databases

eggNOGNOG119843.
HOGENOMHOG000057096.
OMANPFGDQN.
OrthoDBEOG4FXVHB.

Gene expression databases

GenevestigatorP25369.
GermOnlineYCL034W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D1.25.40.90. 1 hit.
InterProIPR008942. ENTH_VHS.
IPR004152. GAT.
[Graphical view]
PfamPF03127. GAT. 1 hit.
[Graphical view]
SUPFAMSSF48464. ENTH_VHS. 1 hit.
PROSITEPS50179. VHS. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio965739.

Entry information

Entry nameLSB5_YEAST
AccessionPrimary (citable) accession number: P25369
Secondary accession number(s): D6VQY1, P87004, Q8NIM9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 27, 2003
Last modified: April 3, 2013
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents