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Protein

LAS seventeen-binding protein 5

Gene

LSB5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the organization of the actin cytoskeleton, fluid phase endocytosis and vesicle trafficking, together with YSC84.1 Publication

GO - Biological processi

  • actin cortical patch localization Source: SGD
  • actin cytoskeleton organization Source: SGD
  • actin filament organization Source: SGD
  • endocytosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29294-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
LAS seventeen-binding protein 5
Short name:
LAS17-binding protein 5
Gene namesi
Name:LSB5
Ordered Locus Names:YCL034W
ORF Names:YCL186, YCL34W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL034W.
SGDiS000000539. LSB5.

Subcellular locationi

GO - Cellular componenti

  • actin cortical patch Source: SGD
  • cell cortex Source: SGD
  • cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354LAS seventeen-binding protein 5PRO_0000084502Add
BLAST

Proteomic databases

MaxQBiP25369.

PTM databases

iPTMnetiP25369.

Interactioni

Subunit structurei

Interacts with SLA1 and LAS17.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARF3P409942EBI-10218,EBI-2824
LAS17Q124463EBI-10218,EBI-10022
SLA1P327905EBI-10218,EBI-17313

Protein-protein interaction databases

BioGridi30949. 14 interactions.
DIPiDIP-6258N.
IntActiP25369. 14 interactions.
MINTiMINT-375039.

Structurei

3D structure databases

ProteinModelPortaliP25369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 161147VHSAdd
BLAST

Sequence similaritiesi

Belongs to the LSB5 family.Curated
Contains 1 VHS domain.Curated

Phylogenomic databases

HOGENOMiHOG000057096.
InParanoidiP25369.
OMAiGERNITS.
OrthoDBiEOG74FF9N.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR008942. ENTH_VHS.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.

Sequencei

Sequence statusi: Complete.

P25369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFLSDHPHT AITETIFRIV SSRDYTLEVE LAPLIQLIKA DHNDYNYTVN
60 70 80 90 100
QEEAARALRK KIKYGNRLQQ SRTLDLLDLF ISQGVKFTVM YNDDKLLQRL
110 120 130 140 150
RGMATNSENS GSGEKYEPRI IKKCAAYAIS WLNYITQNNL ENARAYSGLY
160 170 180 190 200
QLGQTVKQRY SKSSRSRRSG GGSGGRSNFM DDSADDTLYQ SNSLTSADRL
210 220 230 240 250
YRIPQINMNK EAPRIRLIIS DALASAVSLQ NSLIGLPKGK FSTDDEEATS
260 270 280 290 300
KFIQARAIRR KVLRYLQLVT EGEFLGSLIH ANDELVAALT AYDDRSAQDD
310 320 330 340 350
SSDESDHGSY DDGIYDENEQ DNSRYIDSES SEEESLSSYQ PSTISNPFGD

HNKI
Length:354
Mass (Da):39,872
Last modified:June 27, 2003 - v3
Checksum:i35772B575D40BEB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42957.1.
BK006937 Genomic DNA. Translation: DAA07450.1.
PIRiS74282.
RefSeqiNP_009896.2. NM_001178679.1.

Genome annotation databases

EnsemblFungiiCAC42957; CAC42957; CAC42957.
YCL034W; YCL034W; YCL034W.
GeneIDi850323.
KEGGisce:YCL034W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42957.1.
BK006937 Genomic DNA. Translation: DAA07450.1.
PIRiS74282.
RefSeqiNP_009896.2. NM_001178679.1.

3D structure databases

ProteinModelPortaliP25369.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30949. 14 interactions.
DIPiDIP-6258N.
IntActiP25369. 14 interactions.
MINTiMINT-375039.

PTM databases

iPTMnetiP25369.

Proteomic databases

MaxQBiP25369.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAC42957; CAC42957; CAC42957.
YCL034W; YCL034W; YCL034W.
GeneIDi850323.
KEGGisce:YCL034W.

Organism-specific databases

EuPathDBiFungiDB:YCL034W.
SGDiS000000539. LSB5.

Phylogenomic databases

HOGENOMiHOG000057096.
InParanoidiP25369.
OMAiGERNITS.
OrthoDBiEOG74FF9N.

Enzyme and pathway databases

BioCyciYEAST:G3O-29294-MONOMER.

Miscellaneous databases

PROiP25369.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR008942. ENTH_VHS.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames."
    Rad M.R., Luetzenkirchen K., Xu G., Kleinhans U., Hollenberg C.P.
    Yeast 7:533-538(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Gromadka R.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. Valles G., Volckaerts G.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Novel proteins linking the actin cytoskeleton to the endocytic machinery in Saccharomyces cerevisiae."
    Dewar H., Warren D.T., Gardiner F.C., Gourlay C.G., Satish N., Richardson M.R., Andrews P.D., Ayscough K.R.
    Mol. Biol. Cell 13:3646-3661(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE, FUNCTION, INTERACTION WITH SLA1, SUBCELLULAR LOCATION.
  7. "The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex."
    Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B.
    Mol. Biol. Cell 10:3521-3538(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LAS17.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLSB5_YEAST
AccessioniPrimary (citable) accession number: P25369
Secondary accession number(s): D6VQY1, P87004, Q8NIM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 27, 2003
Last modified: June 8, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.