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Protein

[PIN+] prion protein RNQ1

Gene

RNQ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transferable epigenetic modifier which forms a prion responsible for the non-Mendelian trait [PIN+]. The native function of the soluble protein is unknown.2 Publications

Keywords - Molecular functioni

Prion

Enzyme and pathway databases

BioCyciYEAST:G3O-29289-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
[PIN+] prion protein RNQ1
Alternative name(s):
Rich in asparagine and glutamine protein 1
Gene namesi
Name:RNQ1
Ordered Locus Names:YCL028W
ORF Names:YCL181, YCL28W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL028W.
SGDiS000000533. RNQ1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Amyloid, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000973901 – 405[PIN+] prion protein RNQ1Add BLAST405

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki5Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki84Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei143PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP25367.
PRIDEiP25367.

PTM databases

iPTMnetiP25367.

Interactioni

Protein-protein interaction databases

BioGridi30955. 96 interactors.
DIPiDIP-4323N.
IntActiP25367. 21 interactors.
MINTiMINT-516964.

Structurei

3D structure databases

ProteinModelPortaliP25367.
SMRiP25367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 402Prion domain (PrD)Add BLAST250

Domaini

The prion domain (PrD) is a Gln/Asn (Q/N)-rich domain, which is unstructured in its native, soluble form, and which forms a parallel in-register beta-sheet in its amyloid form.

Phylogenomic databases

InParanoidiP25367.
OMAiSNQNYGS.
OrthoDBiEOG092C5AYF.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR011990. TPR-like_helical_dom.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTDKLISEA ESHFSQGNHA EAVAKLTSAA QSNPNDEQMS TIESLIQKIA
60 70 80 90 100
GYVMDNRSGG SDASQDRAAG GGSSFMNTLM ADSKGSSQTQ LGKLALLATV
110 120 130 140 150
MTHSSNKGSS NRGFDVGTVM SMLSGSGGGS QSMGASGLAA LASQFFKSGN
160 170 180 190 200
NSQGQGQGQG QGQGQGQGQG QGSFTALASL ASSFMNSNNN NQQGQNQSSG
210 220 230 240 250
GSSFGALASM ASSFMHSNNN QNSNNSQQGY NQSYQNGNQN SQGYNNQQYQ
260 270 280 290 300
GGNGGYQQQQ GQSGGAFSSL ASMAQSYLGG GQTQSNQQQY NQQGQNNQQQ
310 320 330 340 350
YQQQGQNYQH QQQGQQQQQG HSSSFSALAS MASSYLGNNS NSNSSYGGQQ
360 370 380 390 400
QANEYGRPQQ NGQQQSNEYG RPQYGGNQNS NGQHESFNFS GNFSQQNNNG

NQNRY
Length:405
Mass (Da):42,580
Last modified:April 26, 2004 - v2
Checksum:i8ED9C40A7DF70F7F
GO

Sequence cautioni

The sequence AAA34615 differs from that shown. Reason: Frameshift at position 323.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti181A → T in AAA34615 (PubMed:3302672).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti166 – 169Missing in strain: SCI7.2/b, SCI11.5/a and SCI11.5/c. 4
Natural varianti171 – 172Missing in strain: SCI7.2/a, SCI7.2/d, SCI11.5/b and SCI11.5/d. 2
Natural varianti289 – 299Missing in strain: SCI7.2/b, SCI7.2/d and SCI11.5/c. Add BLAST11
Natural varianti293 – 303Missing in strain: SCI9. Add BLAST11
Natural varianti360Q → H in strain: SCI3, SCI4, SCI7.2/a, SCI7.2/b, SCI7.2/d, SCI11.5/a, SCI11.5/b, SCI11.5/c and SCI11.5/d. 1
Natural varianti372P → PQHNGQQQSNEYGRP in strain: SCI7.2/b, SCI7.2/d and SCI11.5/c. 1
Natural varianti383Q → H in strain: SCI7.2/b,SCI7.2/d and SCI11.5/c. 1
Natural varianti387F → L in strain: SCI4. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16717 Genomic DNA. Translation: AAA34615.1. Frameshift.
X59720 Genomic DNA. Translation: CAC42958.1.
AY028674 Genomic DNA. Translation: AAK26208.1.
AY028675 Genomic DNA. Translation: AAK26209.1.
AY028676 Genomic DNA. Translation: AAK26210.1.
AY028677 Genomic DNA. Translation: AAK26211.1.
AY028678 Genomic DNA. Translation: AAK26212.1.
AY028679 Genomic DNA. Translation: AAK26213.1.
AY028680 Genomic DNA. Translation: AAK26214.1.
AY028681 Genomic DNA. Translation: AAK26215.1.
AY028682 Genomic DNA. Translation: AAK26216.1.
AY028683 Genomic DNA. Translation: AAK26217.1.
AY028684 Genomic DNA. Translation: AAK26218.1.
AY028685 Genomic DNA. Translation: AAK26219.1.
BK006937 Genomic DNA. Translation: DAA07456.1.
PIRiS19355.
RefSeqiNP_009902.2. NM_001178673.1.

Genome annotation databases

EnsemblFungiiCAC42958; CAC42958; CAC42958.
YCL028W; YCL028W; YCL028W.
GeneIDi850329.
KEGGisce:YCL028W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16717 Genomic DNA. Translation: AAA34615.1. Frameshift.
X59720 Genomic DNA. Translation: CAC42958.1.
AY028674 Genomic DNA. Translation: AAK26208.1.
AY028675 Genomic DNA. Translation: AAK26209.1.
AY028676 Genomic DNA. Translation: AAK26210.1.
AY028677 Genomic DNA. Translation: AAK26211.1.
AY028678 Genomic DNA. Translation: AAK26212.1.
AY028679 Genomic DNA. Translation: AAK26213.1.
AY028680 Genomic DNA. Translation: AAK26214.1.
AY028681 Genomic DNA. Translation: AAK26215.1.
AY028682 Genomic DNA. Translation: AAK26216.1.
AY028683 Genomic DNA. Translation: AAK26217.1.
AY028684 Genomic DNA. Translation: AAK26218.1.
AY028685 Genomic DNA. Translation: AAK26219.1.
BK006937 Genomic DNA. Translation: DAA07456.1.
PIRiS19355.
RefSeqiNP_009902.2. NM_001178673.1.

3D structure databases

ProteinModelPortaliP25367.
SMRiP25367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30955. 96 interactors.
DIPiDIP-4323N.
IntActiP25367. 21 interactors.
MINTiMINT-516964.

PTM databases

iPTMnetiP25367.

Proteomic databases

MaxQBiP25367.
PRIDEiP25367.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAC42958; CAC42958; CAC42958.
YCL028W; YCL028W; YCL028W.
GeneIDi850329.
KEGGisce:YCL028W.

Organism-specific databases

EuPathDBiFungiDB:YCL028W.
SGDiS000000533. RNQ1.

Phylogenomic databases

InParanoidiP25367.
OMAiSNQNYGS.
OrthoDBiEOG092C5AYF.

Enzyme and pathway databases

BioCyciYEAST:G3O-29289-MONOMER.

Miscellaneous databases

PROiP25367.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR011990. TPR-like_helical_dom.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNQ1_YEAST
AccessioniPrimary (citable) accession number: P25367
Secondary accession number(s): D6VQY7
, Q8NKJ9, Q8TF94, Q8TFA2, Q8TFC8, Q8TFP4, Q8TFP5, Q8TFP6, Q8TFP7, Q8TFP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: April 26, 2004
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

[PIN+], also known as [RNQ+], is the prion form of RNQ1 (PubMed:10678178). [PIN+] is the result of a conformational change of the cellular RNQ1 protein that becomes self-propagating and infectious. This conformational change generates a form of RNQ1 that assembles into amyloid fibrils (PubMed:17097676). [PIN+] promotes de novo [PSI+] formation upon SUP35 overproduction (cross-seeding) (PubMed:11511345). [PIN+] can be cured by GdnHCl and by deletion of the molecular chaperone HSP104, which is required for [PIN+] propagation (PubMed:10678178).3 Publications
Present with 1140 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.