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P25367 (RNQ1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
[PIN+] prion protein RNQ1
Alternative name(s):
Rich in asparagine and glutamine protein 1
Gene names
Name:RNQ1
Ordered Locus Names:YCL028W
ORF Names:YCL181, YCL28W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length405 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transferable epigenetic modifier which forms a prion responsible for the non-Mendelian trait [PIN+]. The native function of the soluble protein is unknown. Ref.8 Ref.9

Subcellular location

Cytoplasm. Nucleus Ref.10.

Domain

The prion domain (PrD) is a Gln/Asn (Q/N)-rich domain, which is unstructured in its native, soluble form, and which forms a parallel in-register beta-sheet in its amyloid form.

Miscellaneous

[PIN+], also known as [RNQ+], is the prion form of RNQ1 (Ref.8). [PIN+] is the result of a conformational change of the cellular RNQ1 protein that becomes self-propagating and infectious. This conformational change generates a form of RNQ1 that assembles into amyloid fibrils (Ref.12). [PIN+] promotes de novo [PSI+] formation upon SUP35 overproduction (cross-seeding) (Ref.9). [PIN+] can be cured by GdnHCl and by deletion of the molecular chaperone HSP104, which is required for [PIN+] propagation (Ref.8).

Present with 1140 molecules/cell in log phase SD medium.

Sequence caution

The sequence AAA34615.1 differs from that shown. Reason: Frameshift at position 323.

Ontologies

Keywords
   Cellular componentAmyloid
Cytoplasm
Nucleus
   Molecular functionPrion
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytosol

Inferred from direct assay Ref.9. Source: SGD

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 405405[PIN+] prion protein RNQ1
PRO_0000097390

Regions

Region153 – 402250Prion domain (PrD)

Amino acid modifications

Modified residue1431Phosphoserine Ref.13

Natural variations

Natural variant166 – 1694Missing in strain: SCI7.2/b, SCI11.5/a and SCI11.5/c.
Natural variant171 – 1722Missing in strain: SCI7.2/a, SCI7.2/d, SCI11.5/b and SCI11.5/d.
Natural variant289 – 29911Missing in strain: SCI7.2/b, SCI7.2/d and SCI11.5/c.
Natural variant293 – 30311Missing in strain: SCI9.
Natural variant3601Q → H in strain: SCI3, SCI4, SCI7.2/a, SCI7.2/b, SCI7.2/d, SCI11.5/a, SCI11.5/b, SCI11.5/c and SCI11.5/d.
Natural variant3721P → PQHNGQQQSNEYGRP in strain: SCI7.2/b, SCI7.2/d and SCI11.5/c.
Natural variant3831Q → H in strain: SCI7.2/b,SCI7.2/d and SCI11.5/c.
Natural variant3871F → L in strain: SCI4.

Experimental info

Sequence conflict1811A → T in AAA34615. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P25367 [UniParc].

Last modified April 26, 2004. Version 2.
Checksum: 8ED9C40A7DF70F7F

FASTA40542,580
        10         20         30         40         50         60 
MDTDKLISEA ESHFSQGNHA EAVAKLTSAA QSNPNDEQMS TIESLIQKIA GYVMDNRSGG 

        70         80         90        100        110        120 
SDASQDRAAG GGSSFMNTLM ADSKGSSQTQ LGKLALLATV MTHSSNKGSS NRGFDVGTVM 

       130        140        150        160        170        180 
SMLSGSGGGS QSMGASGLAA LASQFFKSGN NSQGQGQGQG QGQGQGQGQG QGSFTALASL 

       190        200        210        220        230        240 
ASSFMNSNNN NQQGQNQSSG GSSFGALASM ASSFMHSNNN QNSNNSQQGY NQSYQNGNQN 

       250        260        270        280        290        300 
SQGYNNQQYQ GGNGGYQQQQ GQSGGAFSSL ASMAQSYLGG GQTQSNQQQY NQQGQNNQQQ 

       310        320        330        340        350        360 
YQQQGQNYQH QQQGQQQQQG HSSSFSALAS MASSYLGNNS NSNSSYGGQQ QANEYGRPQQ 

       370        380        390        400 
NGQQQSNEYG RPQYGGNQNS NGQHESFNFS GNFSQQNNNG NQNRY 

« Hide

References

« Hide 'large scale' references
[1]"Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein."
Trueheart J., Boeke J.D., Fink G.R.
Mol. Cell. Biol. 7:2316-2328(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames."
Rad M.R., Luetzenkirchen K., Xu G., Kleinhans U., Hollenberg C.P.
Yeast 7:533-538(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Valles G., Volckaerts G.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 360.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Prion protein gene polymorphisms in Saccharomyces cerevisiae."
Resende C.G., Outeiro T.F., Sands L., Lindquist S., Tuite M.F.
Mol. Microbiol. 49:1005-1017(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 138-405.
Strain: SCI11.5/a, SCI11.5/b, SCI11.5/c, SCI11.5/d, SCI16, SCI3, SCI4, SCI7.2/a, SCI7.2/b, SCI7.2/d, SCI9 and SCI9*.
[7]"Genetic and environmental factors affecting the de novo appearance of the [PSI+] prion in Saccharomyces cerevisiae."
Derkatch I.L., Bradley M.E., Zhou P., Chernoff Y.O., Liebman S.W.
Genetics 147:507-519(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PRION FORMATION.
[8]"Rnq1: an epigenetic modifier of protein function in yeast."
Sondheimer N., Lindquist S.L.
Mol. Cell 5:163-172(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION AS A PRION, AGGREGATION.
[9]"Prions affect the appearance of other prions: the story of [PIN(+)]."
Derkatch I.L., Bradley M.E., Hong J.Y., Liebman S.W.
Cell 106:171-182(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, AGGREGATION.
[10]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]"'Prion-proof' for [PIN+]: infection with in vitro-made amyloid aggregates of Rnq1p-(132-405) induces [PIN+]."
Patel B.K., Liebman S.W.
J. Mol. Biol. 365:773-782(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PRION PROPAGATION.
[13]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Amyloid of Rnq1p, the basis of the [PIN+] prion, has a parallel in-register beta-sheet structure."
Wickner R.B., Dyda F., Tycko R.
Proc. Natl. Acad. Sci. U.S.A. 105:2403-2408(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 153-405.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16717 Genomic DNA. Translation: AAA34615.1. Frameshift.
X59720 Genomic DNA. Translation: CAC42958.1.
AY028674 Genomic DNA. Translation: AAK26208.1.
AY028675 Genomic DNA. Translation: AAK26209.1.
AY028676 Genomic DNA. Translation: AAK26210.1.
AY028677 Genomic DNA. Translation: AAK26211.1.
AY028678 Genomic DNA. Translation: AAK26212.1.
AY028679 Genomic DNA. Translation: AAK26213.1.
AY028680 Genomic DNA. Translation: AAK26214.1.
AY028681 Genomic DNA. Translation: AAK26215.1.
AY028682 Genomic DNA. Translation: AAK26216.1.
AY028683 Genomic DNA. Translation: AAK26217.1.
AY028684 Genomic DNA. Translation: AAK26218.1.
AY028685 Genomic DNA. Translation: AAK26219.1.
BK006937 Genomic DNA. Translation: DAA07456.1.
PIRS19355.
RefSeqNP_009902.2. NM_001178673.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid30955. 88 interactions.
DIPDIP-4323N.
IntActP25367. 19 interactions.
MINTMINT-516964.

Proteomic databases

MaxQBP25367.
PeptideAtlasP25367.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCL028W; YCL028W; YCL028W.
GeneID850329.
KEGGsce:YCL028W.

Organism-specific databases

CYGDYCL028w.
SGDS000000533. RNQ1.

Phylogenomic databases

OMASNQNYGS.
OrthoDBEOG7X3R3V.

Enzyme and pathway databases

BioCycYEAST:G3O-29289-MONOMER.

Gene expression databases

GenevestigatorP25367.

Family and domain databases

ProtoNetSearch...

Other

NextBio965757.

Entry information

Entry nameRNQ1_YEAST
AccessionPrimary (citable) accession number: P25367
Secondary accession number(s): D6VQY7 expand/collapse secondary AC list , Q8NKJ9, Q8TF94, Q8TFA2, Q8TFC8, Q8TFP4, Q8TFP5, Q8TFP6, Q8TFP7, Q8TFP8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: April 26, 2004
Last modified: May 14, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD