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P25365

- SED4_YEAST

UniProt

P25365 - SED4_YEAST

Protein

Putative guanine nucleotide-exchange factor SED4

Gene

SED4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Putative guanine nucleotide-exchange factor (GEF) involved in the formation or budding of transport vesicles from the ER. Positive regulator of SAR1 probably through inhibition of the GTPase activation by SEC23.3 Publications

    GO - Molecular functioni

    1. Sar GTPase activator activity Source: SGD

    GO - Biological processi

    1. positive regulation of GTPase activity Source: SGD
    2. protein transport Source: UniProtKB-KW
    3. regulation of COPII vesicle coating Source: SGD
    4. vesicle-mediated transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    GTPase activation

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29370-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative guanine nucleotide-exchange factor SED4
    Gene namesi
    Name:SED4
    Ordered Locus Names:YCR067C
    ORF Names:YCR67C, YCR901
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR067c.
    SGDiS000000663. SED4.

    Subcellular locationi

    Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane Curated; Single-pass type II membrane protein Curated
    Note: In the process of transport, SED4 itself may migrate to the Golgi apparatus and function in subsequent transport events.

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of endoplasmic reticulum membrane Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10651065Putative guanine nucleotide-exchange factor SED4PRO_0000051211Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei65 – 651Phosphoserine1 Publication
    Glycosylationi388 – 3881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi620 – 6201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1039 – 10391N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP25365.
    PaxDbiP25365.

    Expressioni

    Gene expression databases

    GenevestigatoriP25365.

    Interactioni

    Protein-protein interaction databases

    BioGridi31043. 33 interactions.
    DIPiDIP-1173N.
    IntActiP25365. 1 interaction.
    MINTiMINT-410531.
    STRINGi4932.YCR067C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25365.
    SMRiP25365. Positions 5-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 346346CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini366 – 1065700LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei347 – 36519Helical; Signal-anchor for type II membrane proteinAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati259 – 29840WD 1Add
    BLAST
    Repeati302 – 34140WD 2Add
    BLAST
    Repeati824 – 833101
    Repeati834 – 843102
    Repeati844 – 853103
    Repeati854 – 863104

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni824 – 863404 X 10 AA tandem repeatsAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1062 – 10654Prevents secretion from ERPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi467 – 47610Poly-Ser
    Compositional biasi579 – 59012Poly-SerAdd
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat SEC12 family.Curated
    Contains 2 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal-anchor, Transmembrane, Transmembrane helix, WD repeat

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00690000103233.
    OrthoDBiEOG7CRV0N.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 1 hit.
    [Graphical view]
    SMARTiSM00320. WD40. 2 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25365-1 [UniParc]FASTAAdd to Basket

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    MSGNSANYDV GYPIYGAKFI NEGTLLVAGG GGQFNSSFPN KITALKVNFQ     50
    KKKHIRRFRE ITLDSIDDAP TSLDCNNNLI LVGCNELFND SSMENVNHHL 100
    RKFVFEQEHL KFVASIDFNR TTDPSVFTKF VYINQRATVA AIASSEVPTV 150
    IRIIDPRNLT ENYEIETGRE VNDLHFAPNG ILLSYITSNS LEVASVRDGN 200
    FVARKTDFDK NLVLSNIRFL NDNTLLVAAS LSNSDGVSLL KLGVSSKGVK 250
    ILKTASFMFD LNGITSMDVS PNKKFVALSS NDNLVAIVSV EKLKLVQLVP 300
    RVHESTITKV TFSPDSRYLA STSMGNTINV LKLSGTSSSI LRNIWKFFLN 350
    FVLLVVLAGA IQLGYKHNVH GFIYKHAHDI YKSKFKENTT IDQGSSSYFT 400
    INDDYRGITE SADIISATDV ASDIETEFSS FDTSTMRTTT EDEQKFVWIS 450
    SSADSQFTSA DIPTSASSSS SSSSSSFYEE SVTNEPIVSS PTSEITKPLA 500
    SPTEPNIVEK PSLPLNSESI DLLSSSSNSI TEYPEPTPDL EEKLSSLIVE 550
    QSESEITTDR ESVSKLLSTE SPSLSHMPSS SSSSLSLSSS LTTSPTTALS 600
    TSTATAVTTT QTNPTNDAAN TSFLDNSKPA STREIYKTKI ITEVITKIEY 650
    RNIPASDSNA EAEQYVTTSS SMLLTPTDTM VSSPVSEIDP IASELERMVE 700
    TPTHSISIAS EFDSVASNLI PNEEILSTSA SQDSISSHPS TFSDSSITSG 750
    FQSIEVSTVT SSVLASESIP SISDSTFSKF HSISEPVSSA IVETATSSFS 800
    KTETKTSRVI AFSTEDSERS SALIDNSEYT SVLADNLEPT SVLADNSEPT 850
    SVLADSSEPT SVFTDAVQSP KTSVGQSSLS ESTNIEGTSM ASMIFSSSGA 900
    SIGALSDIGK GTLSVESASS TVAQPMPGVT TTAPSFVSSP HKISASSIDA 950
    SGFVQKEIMI EVQSSKDSSE AFGVRHKISE NVNTPVSRML TTEMQASGTV 1000
    DVTEDVSLSS EVISALNVEI TSLPNPVAPP QTIAAPLNNN SNTNIVNDDN 1050
    AVAGTVNYAG LHDEL 1065
    Length:1,065
    Mass (Da):114,079
    Last modified:May 1, 1992 - v1
    Checksum:i0F1DAE0D428085A8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42273.1.
    AY693176 Genomic DNA. Translation: AAT93195.1.
    BK006937 Genomic DNA. Translation: DAA07539.1.
    PIRiS19482.
    RefSeqiNP_009993.1. NM_001178778.1.

    Genome annotation databases

    EnsemblFungiiYCR067C; YCR067C; YCR067C.
    GeneIDi850431.
    KEGGisce:YCR067C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42273.1 .
    AY693176 Genomic DNA. Translation: AAT93195.1 .
    BK006937 Genomic DNA. Translation: DAA07539.1 .
    PIRi S19482.
    RefSeqi NP_009993.1. NM_001178778.1.

    3D structure databases

    ProteinModelPortali P25365.
    SMRi P25365. Positions 5-333.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31043. 33 interactions.
    DIPi DIP-1173N.
    IntActi P25365. 1 interaction.
    MINTi MINT-410531.
    STRINGi 4932.YCR067C.

    Proteomic databases

    MaxQBi P25365.
    PaxDbi P25365.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR067C ; YCR067C ; YCR067C .
    GeneIDi 850431.
    KEGGi sce:YCR067C.

    Organism-specific databases

    CYGDi YCR067c.
    SGDi S000000663. SED4.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00690000103233.
    OrthoDBi EOG7CRV0N.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29370-MONOMER.

    Miscellaneous databases

    NextBioi 966015.

    Gene expression databases

    Genevestigatori P25365.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 1 hit.
    [Graphical view ]
    SMARTi SM00320. WD40. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genes that allow yeast cells to grow in the absence of the HDEL receptor."
      Hardwick K.G., Boothroyd J.C., Rudner A.D., Pelham H.R.B.
      EMBO J. 11:4187-4195(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    2. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Sequence of the sup61-RAD18 region on chromosome III of Saccharomyces cerevisiae."
      Benit P., Chanet R., Fabre F., Faye G., Fukuhara H., Sor F.
      Yeast 8:147-153(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 446-1065.
    6. "SED4 encodes a yeast endoplasmic reticulum protein that binds Sec16p and participates in vesicle formation."
      Gimeno R.E., Espenshade P.J., Kaiser C.A.
      J. Cell Biol. 131:325-338(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH SEC16.
    7. "Identification of SEC12, SED4, truncated SEC16, and EKS1/HRD3 as multicopy suppressors of ts mutants of Sar1 GTPase."
      Saito Y., Yamanushi T., Oka T., Nakano A.
      J. Biochem. 125:130-137(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Sed4p functions as a positive regulator of Sar1p probably through inhibition of the GTPase activation by Sec23p."
      Saito-Nakano Y., Nakano A.
      Genes Cells 5:1039-1048(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSED4_YEAST
    AccessioniPrimary (citable) accession number: P25365
    Secondary accession number(s): D6VR70
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3