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P25365

- SED4_YEAST

UniProt

P25365 - SED4_YEAST

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Protein
Putative guanine nucleotide-exchange factor SED4
Gene
SED4, YCR067C, YCR67C, YCR901
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Putative guanine nucleotide-exchange factor (GEF) involved in the formation or budding of transport vesicles from the ER. Positive regulator of SAR1 probably through inhibition of the GTPase activation by SEC23.3 Publications

GO - Molecular functioni

  1. Sar GTPase activator activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. positive regulation of GTPase activity Source: SGD
  2. protein transport Source: UniProtKB-KW
  3. regulation of COPII vesicle coating Source: SGD
  4. vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29370-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative guanine nucleotide-exchange factor SED4
Gene namesi
Name:SED4
Ordered Locus Names:YCR067C
ORF Names:YCR67C, YCR901
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR067c.
SGDiS000000663. SED4.

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein Reviewed prediction
Note: In the process of transport, SED4 itself may migrate to the Golgi apparatus and function in subsequent transport events.2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 346346Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei347 – 36519Helical; Signal-anchor for type II membrane protein
Add
BLAST
Topological domaini366 – 1065700Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of endoplasmic reticulum membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10651065Putative guanine nucleotide-exchange factor SED4
PRO_0000051211Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei65 – 651Phosphoserine1 Publication
Glycosylationi388 – 3881N-linked (GlcNAc...) Reviewed prediction
Glycosylationi620 – 6201N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1039 – 10391N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP25365.
PaxDbiP25365.

Expressioni

Gene expression databases

GenevestigatoriP25365.

Interactioni

Protein-protein interaction databases

BioGridi31043. 33 interactions.
DIPiDIP-1173N.
IntActiP25365. 1 interaction.
MINTiMINT-410531.
STRINGi4932.YCR067C.

Structurei

3D structure databases

ProteinModelPortaliP25365.
SMRiP25365. Positions 5-333.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati259 – 29840WD 1
Add
BLAST
Repeati302 – 34140WD 2
Add
BLAST
Repeati824 – 833101
Repeati834 – 843102
Repeati844 – 853103
Repeati854 – 863104

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni824 – 863404 X 10 AA tandem repeats
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1062 – 10654Prevents secretion from ER Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi467 – 47610Poly-Ser
Compositional biasi579 – 59012Poly-Ser
Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat SEC12 family.
Contains 2 WD repeats.

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix, WD repeat

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00690000103233.
OrthoDBiEOG7CRV0N.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 2 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25365-1 [UniParc]FASTAAdd to Basket

« Hide

MSGNSANYDV GYPIYGAKFI NEGTLLVAGG GGQFNSSFPN KITALKVNFQ     50
KKKHIRRFRE ITLDSIDDAP TSLDCNNNLI LVGCNELFND SSMENVNHHL 100
RKFVFEQEHL KFVASIDFNR TTDPSVFTKF VYINQRATVA AIASSEVPTV 150
IRIIDPRNLT ENYEIETGRE VNDLHFAPNG ILLSYITSNS LEVASVRDGN 200
FVARKTDFDK NLVLSNIRFL NDNTLLVAAS LSNSDGVSLL KLGVSSKGVK 250
ILKTASFMFD LNGITSMDVS PNKKFVALSS NDNLVAIVSV EKLKLVQLVP 300
RVHESTITKV TFSPDSRYLA STSMGNTINV LKLSGTSSSI LRNIWKFFLN 350
FVLLVVLAGA IQLGYKHNVH GFIYKHAHDI YKSKFKENTT IDQGSSSYFT 400
INDDYRGITE SADIISATDV ASDIETEFSS FDTSTMRTTT EDEQKFVWIS 450
SSADSQFTSA DIPTSASSSS SSSSSSFYEE SVTNEPIVSS PTSEITKPLA 500
SPTEPNIVEK PSLPLNSESI DLLSSSSNSI TEYPEPTPDL EEKLSSLIVE 550
QSESEITTDR ESVSKLLSTE SPSLSHMPSS SSSSLSLSSS LTTSPTTALS 600
TSTATAVTTT QTNPTNDAAN TSFLDNSKPA STREIYKTKI ITEVITKIEY 650
RNIPASDSNA EAEQYVTTSS SMLLTPTDTM VSSPVSEIDP IASELERMVE 700
TPTHSISIAS EFDSVASNLI PNEEILSTSA SQDSISSHPS TFSDSSITSG 750
FQSIEVSTVT SSVLASESIP SISDSTFSKF HSISEPVSSA IVETATSSFS 800
KTETKTSRVI AFSTEDSERS SALIDNSEYT SVLADNLEPT SVLADNSEPT 850
SVLADSSEPT SVFTDAVQSP KTSVGQSSLS ESTNIEGTSM ASMIFSSSGA 900
SIGALSDIGK GTLSVESASS TVAQPMPGVT TTAPSFVSSP HKISASSIDA 950
SGFVQKEIMI EVQSSKDSSE AFGVRHKISE NVNTPVSRML TTEMQASGTV 1000
DVTEDVSLSS EVISALNVEI TSLPNPVAPP QTIAAPLNNN SNTNIVNDDN 1050
AVAGTVNYAG LHDEL 1065
Length:1,065
Mass (Da):114,079
Last modified:May 1, 1992 - v1
Checksum:i0F1DAE0D428085A8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42273.1.
AY693176 Genomic DNA. Translation: AAT93195.1.
BK006937 Genomic DNA. Translation: DAA07539.1.
PIRiS19482.
RefSeqiNP_009993.1. NM_001178778.1.

Genome annotation databases

EnsemblFungiiYCR067C; YCR067C; YCR067C.
GeneIDi850431.
KEGGisce:YCR067C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42273.1 .
AY693176 Genomic DNA. Translation: AAT93195.1 .
BK006937 Genomic DNA. Translation: DAA07539.1 .
PIRi S19482.
RefSeqi NP_009993.1. NM_001178778.1.

3D structure databases

ProteinModelPortali P25365.
SMRi P25365. Positions 5-333.
ModBasei Search...

Protein-protein interaction databases

BioGridi 31043. 33 interactions.
DIPi DIP-1173N.
IntActi P25365. 1 interaction.
MINTi MINT-410531.
STRINGi 4932.YCR067C.

Proteomic databases

MaxQBi P25365.
PaxDbi P25365.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR067C ; YCR067C ; YCR067C .
GeneIDi 850431.
KEGGi sce:YCR067C.

Organism-specific databases

CYGDi YCR067c.
SGDi S000000663. SED4.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00690000103233.
OrthoDBi EOG7CRV0N.

Enzyme and pathway databases

BioCyci YEAST:G3O-29370-MONOMER.

Miscellaneous databases

NextBioi 966015.

Gene expression databases

Genevestigatori P25365.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 1 hit.
[Graphical view ]
SMARTi SM00320. WD40. 2 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genes that allow yeast cells to grow in the absence of the HDEL receptor."
    Hardwick K.G., Boothroyd J.C., Rudner A.D., Pelham H.R.B.
    EMBO J. 11:4187-4195(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Sequence of the sup61-RAD18 region on chromosome III of Saccharomyces cerevisiae."
    Benit P., Chanet R., Fabre F., Faye G., Fukuhara H., Sor F.
    Yeast 8:147-153(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 446-1065.
  6. "SED4 encodes a yeast endoplasmic reticulum protein that binds Sec16p and participates in vesicle formation."
    Gimeno R.E., Espenshade P.J., Kaiser C.A.
    J. Cell Biol. 131:325-338(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH SEC16.
  7. "Identification of SEC12, SED4, truncated SEC16, and EKS1/HRD3 as multicopy suppressors of ts mutants of Sar1 GTPase."
    Saito Y., Yamanushi T., Oka T., Nakano A.
    J. Biochem. 125:130-137(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Sed4p functions as a positive regulator of Sar1p probably through inhibition of the GTPase activation by Sec23p."
    Saito-Nakano Y., Nakano A.
    Genes Cells 5:1039-1048(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSED4_YEAST
AccessioniPrimary (citable) accession number: P25365
Secondary accession number(s): D6VR70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi